We narrowed to 16,615 results for: puromycin

1. CRISPRi-CENPB-g1

   Plasmid

   #120209

   Purpose

   CENPB CRISPRi guide RNA 1

   Depositor

   David Bartel

   Article

   Wu et al Cell. 2017 May 18;169(5):905-917.e11. do

   Insert

   CENPB CRISPRi guide RNA
   

   Use

   CRISPR and Lentiviral

   Available Since

   March 3, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. p663-UBC-miniTurbo-V5-BRSK1_IDG-K

   Plasmid

   #135269

   Purpose

   Gateway destination clone of BRSK1 (human) tagged with N-terminal miniTurbo-V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomics

   Depositor

   Ben Major

   Insert

   miniTurbo-V5-BRSK1 (BRSK1 Human)
   

   Use

   Lentiviral; Gateway destination

   Tags

   V5-miniTurbo

   Expression

   Mammalian

   Promoter

   Ubiquitin

   Available Since

   Jan. 15, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. p667-UBC-BRSK1-V5-miniTurbo_IDG-K

   Plasmid

   #135258

   Purpose

   Gateway destination clone of BRSK1 (human) tagged with C-terminal V5-miniTurbo for generating protein-proximity networks using proximity-dependent biotinylation proteomics

   Depositor

   Ben Major

   Insert

   BRSK1-V5-miniTurbo (BRSK1 Human)
   

   Use

   Lentiviral; Gateway destination

   Tags

   V5-miniTurbo

   Expression

   Mammalian

   Promoter

   Ubiquitin

   Available Since

   Jan. 15, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. p667-UBC-BRSK1-V5_IDG-K

   Plasmid

   #135259

   Purpose

   Gateway destination clone of BRSK1 (human) tagged with C-terminal V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomics

   Depositor

   Ben Major

   Insert

   BRSK1-V5 (BRSK1 Human)
   

   Use

   Lentiviral; Gateway destination

   Tags

   V5

   Expression

   Mammalian

   Promoter

   Ubiquitin

   Available Since

   Jan. 15, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. Fuw PBRM1 mBD4

   Plasmid

   #121108

   Purpose

   conditional expression

   Depositor

   Emily Dykhuizen

   Article

   Porter et al J Biol Chem. 2017 Jan 4. pii: jbc.M116.7

   Insert

   PBRM1 (N601A) (PBRM1 Human)
   

   Use

   Lentiviral

   Mutation

   PBRM1 (N601A)

   Available Since

   March 12, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. Fuw PBRM1 mBD5

   Plasmid

   #121109

   Purpose

   conditional expression

   Depositor

   Emily Dykhuizen

   Article

   Porter et al J Biol Chem. 2017 Jan 4. pii: jbc.M116.7

   Insert

   PBRM1 (N739A) (PBRM1 Human)
   

   Use

   Lentiviral

   Mutation

   PBRM1 (N739A)

   Available Since

   Feb. 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. Fuw PBRM1 mBD1

   Plasmid

   #121105

   Purpose

   conditional expression

   Depositor

   Emily Dykhuizen

   Article

   Porter et al J Biol Chem. 2017 Jan 4. pii: jbc.M116.7

   Insert

   PBRM1 (Y127A) (PBRM1 Human)
   

   Use

   Lentiviral

   Mutation

   PBRM1 (Y127A)

   Available Since

   Feb. 8, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. Fuw PBRM1 mBD3

   Plasmid

   #121107

   Purpose

   conditional expression

   Depositor

   Emily Dykhuizen

   Article

   Porter et al J Biol Chem. 2017 Jan 4. pii: jbc.M116.7

   Insert

   PBRM1 (N463A) (PBRM1 Human)
   

   Use

   Lentiviral

   Mutation

   PBRM1 (N463A)

   Available Since

   Jan. 23, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. Fuw PBRM1 mBD2

   Plasmid

   #121106

   Purpose

   conditional expression

   Depositor

   Emily Dykhuizen

   Article

   Porter et al J Biol Chem. 2017 Jan 4. pii: jbc.M116.7

   Insert

   PBRM1 (N263A) (PBRM1 Human)
   

   Use

   Lentiviral

   Mutation

   PBRM1 (N263A)

   Available Since

   Jan. 23, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pBABE.puro/TO/Flag/HuR.S221D

    Plasmid

    #110347

    Purpose

    Doxycycline inducible mammalian retroviral expression of HuR (S221D) with FLAG tag

    Depositor

    Sandra Martha Gomes Dias

    Insert

    ELAV like RNA binding protein 1 (ELAVL1 Human)
    

    Use

    Retroviral

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    S221D, P237S

    Promoter

    gag

    Available Since

    Aug. 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pBABE.puro/TO/Flag/HuR.S221D

    Plasmid

    #110355

    Purpose

    Doxycycline inducible mammalian retroviral expression of HuR (S221D) with FLAG and mKO2 tags

    Depositor

    Sandra Martha Gomes Dias

    Insert

    ELAV like RNA binding protein 1 (ELAVL1 Human)
    

    Use

    Retroviral

    Tags

    FLAG and mKO2

    Expression

    Mammalian

    Mutation

    S221D, P237S

    Promoter

    gag

    Available Since

    June 22, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. CJ26 (miR-1003 3'ssAG->GA)

    Plasmid

    #17765

    Depositor

    David Bartel

    Article

    Ruby et al Nature. 2007 Jul 5. 448(7149):83-6.

    Insert

    dme-miR-1003 minigene 3'ssAG->GA
    

    Use

    Drosophila metallothionein gene promoter drives e…

    Expression

    Insect

    Mutation

    splice site mutation

    Available Since

    April 15, 2008

    Availability

    Academic Institutions and Nonprofits only

13. CJ50 (cg11094 utr mut)

    Plasmid

    #17778

    Depositor

    David Bartel

    Article

    Ruby et al Nature. 2007 Jul 5. 448(7149):83-6.

    Insert

    cg11094 utr fragment mut
    

    Use

    Luciferase; Drosophila metallothionein gene promo…

    Tags

    renilla luciferase

    Expression

    Insect

    Mutation

    mutant version

    Available Since

    April 15, 2008

    Availability

    Academic Institutions and Nonprofits only

14. CJ58 (cg6551 utr mut)

    Plasmid

    #17784

    Depositor

    David Bartel

    Article

    Ruby et al Nature. 2007 Jul 5. 448(7149):83-6.

    Insert

    cg6551 utr fragment mut
    

    Use

    Luciferase; Drosophila metallothionein gene promo…

    Tags

    renilla luciferase

    Expression

    Insect

    Mutation

    mutant version

    Available Since

    April 15, 2008

    Availability

    Academic Institutions and Nonprofits only

15. pEH_AAV_TERT_prom_-124C>T_C7C_1.8kb

    Plasmid

    #183080

    Purpose

    rAAV transfer plasmid with ITRs flanking: (1) TERT -124C>T promoter and exon 1 C7C (C21>T21), 1.8kb homologous recombination donor template with ~900 bp homology arms, and (2) PGK-Puro.

    Depositor

    Aviv Regev

    Article

    Hodis et al Science. 2022 Apr 29;376(6592):eabi8175.

    Insert

    TERT -124C>T promoter and exon 1 C7C (C21>T21), 1.8kb homologous recombination donor template with ~900 bp homology arms (TERT Human)
    

    Use

    AAV; Homologous recombination donor template

    Mutation

    -124C>T promoter, Exon 1 C7C (C21>T21) sile…

    Promoter

    None for primary insert (Though partial TERT prom…

    Available Since

    June 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pEH_AAV_TERT_prom_-146C>T_C7C_1.8kb

    Plasmid

    #183081

    Purpose

    rAAV transfer plasmid with ITRs flanking: (1) TERT -146C>T promoter and exon 1 C7C (C21>T21), 1.8kb homologous recombination donor template with ~900 bp homology arms, and (2) PGK-Puro.

    Depositor

    Aviv Regev

    Article

    Hodis et al Science. 2022 Apr 29;376(6592):eabi8175.

    Insert

    TERT -146C>T promoter and exon 1 C7C (C21>T21), 1.8kb homologous recombination donor template with ~900 bp homology arms (TERT Human)
    

    Use

    AAV; Homologous recombination donor template

    Mutation

    -146C>T promoter, Exon 1 C7C (C21>T21) sile…

    Promoter

    None for primary insert (Though partial TERT prom…

    Available Since

    June 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pEH_AAV_TERT_prom_C7C_1.8kb

    Plasmid

    #183079

    Purpose

    rAAV transfer plasmid with ITRs flanking: (1) TERT wildtype promoter and exon 1 C7C (C21>T21), 1.8kb homologous recombination donor template with ~900 bp homology arms, and (2) PGK-Puro.

    Depositor

    Aviv Regev

    Article

    Hodis et al Science. 2022 Apr 29;376(6592):eabi8175.

    Insert

    TERT wildtype promoter and exon 1 C7C (C21>T21), 1.8kb homologous recombination donor template with ~900 bp homology arms (TERT Human)
    

    Use

    AAV; Homologous recombination donor template

    Mutation

    Wildtype promoter, Exon 1 C7C (C21>T21) silent…

    Promoter

    None for primary insert (Though partial TERT prom…

    Available Since

    June 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pLenti CMV Puro DEST (w118-1) CRIP2

    Plasmid

    #107509

    Purpose

    Expressing CRIP2, puromycin resistant

    Depositor

    Alejandro Gutierrez

    Article

    Aries et al J Exp Med. 2018 Nov 7. pii: jem.20180570

    Insert

    CRIP2 (codon optimized) (CRIP2 Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Mutation

    Codon optimized sequence

    Promoter

    CMV

    Available Since

    Nov. 7, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. retro-gfpIkkb-puro vector

    Plasmid

    #58251

    Purpose

    Retroviral expression vector encoding bicistronic EGFP-IKKbeta and Puromycin cassette

    Depositor

    Georgios Stathopoulos

    Article

    Giannou et al J Clin Invest. 2015 Jun;125(6):2317-34.

    Insert

    Ikkβ (Ikbkb Mouse)
    

    Use

    Retroviral

    Expression

    Mammalian

    Available Since

    March 30, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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20. pEH_AAV_BRAF_ex15_V600E_S607S_1.8kb

    Plasmid

    #183078

    Purpose

    rAAV transfer plasmid with ITRs flanking: (1) BRAF V600E (T>A), S607S (TCC>AGT), 1.8kb homologous recombination donor template centered on BRAF exon 15 with ~900 bp homology arms, and (2) PGK-Puro.

    Depositor

    Aviv Regev

    Article

    Hodis et al Science. 2022 Apr 29;376(6592):eabi8175.

    Insert

    BRAF Exon 15, 1.8kb homologous recombination donor, ~900 bp homology arms (BRAF Human)
    

    Use

    AAV; Homologous recombination donor template

    Mutation

    V600E (T>A), S607S (TCC>AGT)

    Promoter

    None for primary insert. PGK for puromycin resist…

    Available Since

    June 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge