Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

Filter By

We narrowed to 17,879 results for: puromycin

Clear keywords
Showing: 2581 - 2600 of 17879 results

  1. CROPseq-multi-v2-SpCas9-P2A-Puro-BsmBI_entry (pRW1512)

    Plasmid
    #225756
    Purpose
    Entry vector to clone sgRNA(s) into the CROPseq-multi-v2 construct with expression of SpCas9 and Puromycin resistance; v2 is compatible with T7 in vitro transcription detection
    Depositor
    Paul Blainey
    Publication
    Walton et al bioRxiv [Preprint]. 2024 Mar 17:2024.03.
    Type
    Empty backbone
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Available since
    Sept. 20, 2024
    Availability
    Academic Institutions and Nonprofits only
    225756-plasmid-map-sequence-id-446064

    Enlarge

  3. pEXPqcxip-hFTSJ3-FLAG

    Plasmid
    #165463
    Purpose
    Human FTSJ3 retroviral mammalian expression vector. FLAG/Gateway modified pQCXIP vector with C-terminal FLAG epitope and bicistronic puromycin expression.
    Depositor
    Martin Dorf
    Publication
    Li et al Proc Natl Acad Sci U S A. 2014 May 13;11
    Insert
    FtsJ RNA 2'-O-methyltransferase 3 (FTSJ3 Human)
    Use
    Retroviral; Gateway
    Tags
    FLAG
    Expression
    Mammalian
    Promoter
    CMV/MSV, 5'LTR
    Available since
    March 17, 2021
    Availability
    Academic Institutions and Nonprofits only
    165463-plasmid-map-sequence-id-327804

    Enlarge

  4. B52_puro_empty_gRNA

    Plasmid
    #197557
    Purpose
    sites for cloning two gRNA with puromycin cassette
    Depositor
    Darius Bägli
    Type
    Empty backbone
    Use
    Empty grna backbone with puromycin resistance gene
    Expression
    Mammalian
    Promoter
    U6 for gRNA, CMV for puromycin resistance.
    Available since
    March 27, 2023
    Availability
    Academic Institutions and Nonprofits only
    197557-plasmid-map-sequence-id-388707

    Enlarge

  6. pSBtet-Pur-dCas9-KRAB-MeCP2_hU6-SapI

    Plasmid
    #196084
    Purpose
    (Empty Backbone) Inducible CRISPRi vector conferring puromycin resistance, ready for insertion of gRNA targeting gene of interest using SapI restriction sites.
    Depositor
    Joanna Bereta
    Publication
    Czarnek et al Mol Biotechnol. 2023 Jan 28. doi: 10.100
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Inserts
    dCas9-KRAB-MeCP2 repressor
    hU6 promoter and gRNA scaffold
    Use
    CRISPR
    Expression
    Mammalian
    Mutation
    Catalytically dead mutant of the Cas9 endonucleas…
    Available since
    March 30, 2023
    Availability
    Academic Institutions and Nonprofits only
    196084-plasmid-map-sequence-id-388993

    Enlarge

  8. pPyCAG-EGFP-TM-IRES-Pac

    Plasmid
    #183603
    Purpose
    Mammalian expression vector for membrane-tethered EGFP from the CAG promoter. Confers puromycin resistance.
    Depositor
    Sally Lowell
    Publication
    Malaguti et al Development. 2022 May 26. pii: 275525. d
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    EGFP-TM
    Tags
    Human PDGFRB transmembrane domain and Mouse IgGK …
    Expression
    Mammalian
    Promoter
    CAG
    Available since
    June 3, 2022
    Availability
    Academic Institutions and Nonprofits only
    183603-plasmid-map-sequence-id-361589

    Enlarge

  9. pPyPGK-EGFP-TM-IRES-Pac

    Plasmid
    #183604
    Purpose
    Mammalian expression vector for membrane-tethered EGFP from the mouse Pgk1 promoter. Confers puromycin resistance.
    Depositor
    Sally Lowell
    Publication
    Malaguti et al Development. 2022 May 26. pii: 275525. d
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    EGFP-TM
    Tags
    Human PDGFRB transmembrane domain and Mouse IgGK …
    Expression
    Mammalian
    Promoter
    Mouse Pgk1
    Available since
    June 3, 2022
    Availability
    Academic Institutions and Nonprofits only
    183604-plasmid-map-sequence-id-361590

    Enlarge

  13. PB-T3G-ERP2

    Plasmid
    #175188
    Purpose
    All-in-one piggyBac transposon vector for dox-inducible expression of Golden Gate cloned elements (hEF1a-driven rtTA and puromycin resistance)
    Depositor
    George Church
    Publication
    Kramme et al Cell Rep Methods. 2021 Sep 27;1(6):10008
    Type
    Empty backbone
    Use
    Synthetic Biology
    Expression
    Mammalian
    Promoter
    TRE3G
    Available since
    Oct. 3, 2021
    Availability
    Academic Institutions and Nonprofits only
    175188-plasmid-map-sequence-id-344441

    Enlarge

  14. pLpC-Reg-L-IFP-F[2]+Venus (mammalian)

    Plasmid
    #52903
    Purpose
    Binary retroviral pl. (puromycin). Reg:Linker (GGGGS)X2+IFP-F[2] (PacI/EcoRI). In the second MCS, the Venus fluorescent protein is cloned in between PmeI/SalI.Confers ampicillin resistance to DH5α.
    Depositor
    Stephen Michnick
    Publication
    Tchekanda et al Nat Methods. 2014 Jun;11(6):641-4. doi:
    Insert
    Reg-L-IFP-F[2]+Venus
    Use
    Retroviral
    Tags
    L-IFP-F[2]
    Expression
    Mammalian
    Promoter
    Unknown
    Available since
    June 5, 2014
    Availability
    Academic Institutions and Nonprofits only

  15. pLpC-Cat-L-IFP-F[1]+Plum (mammalian)

    Plasmid
    #52905
    Purpose
    Binary retroviral pl. (puromycin). Cat(PacI/ClaI):Linker (GGGGS)X2+IFP-F[2]. In the second MCS, the Plum fluorescent protein is cloned in between PmeI/SalI.Confers ampicillin resistance to DH5α.
    Depositor
    Stephen Michnick
    Publication
    Tchekanda et al Nat Methods. 2014 Jun;11(6):641-4. doi:
    Insert
    Cat-L-IFP-F[1]+Plum
    Use
    Retroviral
    Tags
    L-IFP-F[1]
    Expression
    Mammalian
    Promoter
    Unknown
    Available since
    June 5, 2014
    Availability
    Academic Institutions and Nonprofits only

  19. pMaCTag-P15

    Plasmid
    #120026
    Purpose
    Template for C-terminal PCR tagging of mammalian genes (Puromycin selection) with miRFP670
    Depositor
    Michael Knop
    Publication
    Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    miRFP670
    Use
    Pcr template
    Tags
    miRFP670
    Promoter
    None
    Available since
    Jan. 8, 2019
    Availability
    Academic Institutions and Nonprofits only
    120026-plasmid-map-sequence-id-232633

    Enlarge

Showing: 2581 - 2600 of 17879 results