167,433 results

1. pAAV-Syn-DIO-PinkyCaMP

   Plasmid

   #232857

   Purpose

   An AAV vector expressing the mScarlet based calcium indicator PinkyCaMP under Syn promoter in a Cre-dependent manner (DIO, double floxed inverted open reading frame)

   Depositor

   Olivia Masseck

   Article

   Fink et al bioRxiv [Preprint]. 2025 Jan 11:2024.12.

   Insert

   PinkyCaMP
   

   Use

   AAV

   Expression

   Mammalian

   Promoter

   hSyn

   Available Since

   Feb. 27, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. pNIC28-Bsa4

   Plasmid

   #26103

   Purpose

   SGC Empty backbone for bacterial expression

   Depositor

   Opher Gileadi

   Article

   Savitsky et al J Struct Biol. 2010 Oct;172(1):3-13. doi

   Type

   Empty backbone

   Tags

   6xHis and TEV cleavage site

   Expression

   Bacterial

   Promoter

   T7-lacO (lactose/IPTG inducible)

   Available Since

   Oct. 18, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLEX_307

   Plasmid

   #41392

   Purpose

   Constitutive lentiviral expression, SV40-puro; EF1a-gateway-V5 tag

   Depositor

   David Root

   Has Service

   Cloning Grade DNA

   Type

   Empty backbone

   Use

   Lentiviral; Gateway destination vector

   Tags

   V5

   Expression

   Mammalian

   Promoter

   EF1a

   Available Since

   Nov. 30, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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4. pHAGE-mt-mKeima-P2A-FRB-Fis1

   Plasmid

   #135295

   Purpose

   stably express both mKeima and FRB-Fis1 for CID induced mitophagy in mammalian cells

   Depositor

   Richard Youle

   Article

   Vargas et al Mol Cell. 2019 Apr 18;74(2):347-362.e6.

   Insert

   mt-mKeima-P2A-FRB-Fis1 (FIS1 Human)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Mutation

   mt-mKeima is fused with FRB-Fis1 via P2A seqeunce

   Available Since

   Jan. 30, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAAV-hSyn1-FLEX-CybSEP2-WPRE

   Plasmid

   #207661

   Purpose

   Monitoring neuropeptide release

   Depositor

   Sung Han

   Article

   Kim et al bioRxiv 2023.01.19.524797

   Insert

   Cytochrome b-561 (Cyb561 Mouse)
   

   Use

   AAV

   Mutation

   Histidine 86 and 159 to alanines, inserted 2 X SE…

   Promoter

   hSynapsin

   Available Since

   Dec. 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAAV.Syn.Flex.NES-jRGECO1a.WPRE.SV40 (AAV1)

   Viral Prep

   #100853-AAV1

   Purpose

   Ready-to-use AAV1 particles produced from pAAV.Syn.Flex.NES-jRGECO1a.WPRE.SV40 (#100853). In addition to the viral particles, you will also receive purified pAAV.Syn.Flex.NES-jRGECO1a.WPRE.SV40 plasmid DNA. Synapsin-driven, Cre-dependent GECO1a calcium sensor. These AAV preparations are suitable purity for injection into animals.

   Depositor

   Douglas Kim, GENIE Project

   Article

   Dana et al eLife. 2016 Mar 24;5. pii: e12727. doi:

   Promoter

   Syn

   Available Since

   Feb. 28, 2018

   Availability

   Academic Institutions and Nonprofits only

7. pAAV-CAG-GFP (AAV PHP.eB)

   Viral Prep

   #37825-PHPeB

   Purpose

   Ready-to-use AAV PHP.eB particles produced from pAAV-CAG-GFP (#37825). In addition to the viral particles, you will also receive purified pAAV-CAG-GFP plasmid DNA. CAG-driven GFP expression. These AAV were produced with the PHPeB serotype, which permits efficient transduction of the central nervous system. These AAV preparations are suitable purity for injection into animals.

   Depositor

   Edward Boyden

   Promoter

   CAG

   Tags

   GFP

   Available Since

   May 4, 2018

   Availability

   Academic Institutions and Nonprofits only

8. eSrtA(4S-9) pET29b

   Plasmid

   #75146

   Purpose

   reprogrammed sortase A variant that recognizes alternative substrate LPXSG with high activity and specificity

   Depositor

   David Liu

   Article

   Dorr et al Proc Natl Acad Sci U S A. 2014 Sep 16;11

   Insert

   reprogrammed sortase A variant
   

   Tags

   6x His

   Expression

   Bacterial

   Mutation

   N98D S102C A104V A118T F122A K134R F144L I182V E1…

   Promoter

   T7

   Available Since

   Aug. 10, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. DLX2-hygro

   Plasmid

   #97330

   Purpose

   Generating lentiviruses to express Dlx2 and hygromycin resistance

   Depositor

   Marius Wernig

   Article

   Yang et al Nat Methods. 2017 May 15. doi: 10.1038/n

   Insert

   DLX2 (Dlx2 Mouse)
   

   Use

   Lentiviral

   Promoter

   TetO

   Available Since

   Aug. 10, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. pREDawn-AmpR-MCS

    Plasmid

    #188978

    Purpose

    For red light-induced bacterial expression of gene of interest (AmpR)

    Depositor

    Heikki Takala

    Article

    Multamaki et al ACS Synth Biol. 2022 Aug 23. doi: 10.102

    Type

    Empty backbone

    Expression

    Bacterial

    Available Since

    Nov. 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCMV-T7-ABE8e-nSpRY-P2A-EGFP (KAC1069)

    Plasmid

    #185912

    Purpose

    pCMV and pT7 plasmid encoding human codon optimized ABE8e A-to-G base editor with nicakse SpRY(D10A/A61R/L1111R/D1135L/S1136W/G1218K/E1219Q/N1317R/A1322R/R1333P/R1335Q/T1337R) and P2A-EGFP

    Depositor

    Benjamin Kleinstiver

    Article

    Alves et al Nat Biomed Eng. 2023 Dec 6. doi: 10.1038

    Insert

    human codon optimized ABE8e-nSpRY-P2A-EGFP
    

    Use

    In vitro transcription; t7 promoter

    Tags

    BPNLS and BPNLS-3xFLAG-P2A-EGFP

    Expression

    Mammalian

    Mutation

    nSpRY=D10A/A61R/L1111R/D1135L/S1136W/G1218K/E1219…

    Promoter

    CMV and T7

    Available Since

    July 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pBB540

    Plasmid

    #27393

    Depositor

    Bernd Bukau

    Article

    de Marco et al BMC Biotechnol. 2007 Jun 12;7:32. doi: 1

    Insert

    grpE, clpB
    

    Expression

    Bacterial

    Available Since

    April 26, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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13. 3xHA-TurboID_pAS31

    Plasmid

    #118220

    Purpose

    expresses 3xHA-tagged TurboID in C. elegans intestine

    Depositor

    Jessica Feldman

    Article

    Branon et al Nat Biotechnol. 2018 Aug 20. pii: nbt.42

    Insert

    TurboID (BirA mutant)
    

    Tags

    3x HA tag

    Expression

    Worm

    Mutation

    Q65P, I87V, R118S, E140K, Q141R, S150G, L151P, V1…

    Promoter

    ges-1p

    Available Since

    Jan. 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pAAV.Syn.Flex.GCaMP6s.WPRE.SV40 (AAV9)

    Viral Prep

    #100845-AAV9

    Purpose

    Ready-to-use AAV9 particles produced from pAAV.Syn.Flex.GCaMP6s.WPRE.SV40 (#100845). In addition to the viral particles, you will also receive purified pAAV.Syn.Flex.GCaMP6s.WPRE.SV40 plasmid DNA. Syn-driven, Cre-dependent GCaMP6s calcium sensor. These AAV preparations are suitable purity for injection into animals.

    Depositor

    Douglas Kim, GENIE Project

    Article

    Chen et al Nature. 2013 Jul 18;499(7458):295-300. d

    Promoter

    Syn

    Available Since

    May 17, 2018

    Availability

    Academic Institutions and Nonprofits only

15. TetO-Ascl1-puro

    Plasmid

    #97329

    Purpose

    Generating lentiviruses to express Ascl1 and puromycin resistance

    Depositor

    Marius Wernig

    Article

    Yang et al Nat Methods. 2017 May 15. doi: 10.1038/n

    Insert

    Ascl1 (Ascl1 Mouse)
    

    Use

    Lentiviral

    Promoter

    TET-ON

    Available Since

    July 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. GFP-PKCA WT

    Plasmid

    #195202

    Purpose

    Mammalian expression vector for GFP tagged PKCA.

    Depositor

    Peter Parker, David Bryant

    Article

    Cameron et al Nat Struct Mol Biol. 2009 Jun;16(6):624-

    Insert

    PRKCA (PRKCA Human)
    

    Expression

    Mammalian

    Available Since

    March 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pAAV-FLEX-SaCas9-U6-sgRNA

    Plasmid

    #124844

    Purpose

    Vector for Cre-dependent expression of SaCas9

    Depositor

    Larry Zweifel

    Article

    Hunker et al Cell Rep. 2020 Mar 24;30(12):4303-4316.e

    Insert

    SaCas9, gRNA scaffold
    

    Use

    AAV, CRISPR, and Mouse Targeting

    Tags

    NLS and NLS-3xHA

    Available Since

    May 6, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. pBB542

    Plasmid

    #27395

    Depositor

    Bernd Bukau

    Article

    de Marco et al BMC Biotechnol. 2007 Jun 12;7:32. doi: 1

    Insert

    dnaK, dnaJ, groESL
    

    Expression

    Bacterial

    Available Since

    Sept. 7, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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19. Dual sgRNA CRISPRa Library

    Pooled Library

    #1000000193

    Depositor

    Jonathan Weissman

    Available Since

    Sept. 30, 2022

    Availability

    Academic Institutions and Nonprofits only

20. pEVOL-pAzF[TAG]

    Plasmid

    #164579

    Purpose

    Machinery plasmid containing two copies of the Mj pCNFRS and cognate tRNA for incorporation of pAzF, pCNF, or pENF at TAG codons

    Depositor

    Ryan Mehl

    Article

    Bednar et al ACS Chem Biol. 2021 Nov 19;16(11):2612-2

    Inserts

    Mj pCNFRS[TAG] (aaRS1)
    Mj pCNFRS[TAG] (aaRS2)
    Mj tRNA[TAG]
    

    Expression

    Bacterial

    Mutation

    Y32L L65V F108W Q109M D158G I159P

    Promoter

    araBAD, glnS, and proK

    Available Since

    May 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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