We narrowed to 32,166 results for: uros

1. pRetro-XT-PAK2-CFP(puro)-T402A

   Plasmid

   #81179

   Purpose

   Retroviral vector for expression of kinase inactive PAK2 with a CFP fusion

   Depositor

   Angeliki Malliri

   Article

   Whalley et al Nat Commun. 2015 Jun 16;6:7437. doi: 10.

   Insert

   p21 (RAC1) activated kinase 2 (PAK2 Human)
   

   Use

   Retroviral

   Tags

   CFP

   Mutation

   T402A

   Promoter

   P-tight TRE

   Available Since

   Aug. 9, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pMSCV-FLAG-2xSTREP-CCND2-Puro

   Plasmid

   #172626

   Purpose

   Retroviral vector for the constitutive expression of FLAG-2xSTREP-tagged cyclin D2 and a puromycin resistance marker in mammalian cells

   Depositor

   Michele Pagano

   Article

   Simoneschi et al Nature. 2021 Apr;592(7856):789-793. doi:

   Insert

   CCND2 (CCND2 Human)
   

   Use

   Retroviral

   Tags

   FLAG-2xSTREP

   Expression

   Mammalian

   Available Since

   Oct. 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. Direct-seq_lentiGuide-Puro-Loop2-8A8G

   Plasmid

   #157983

   Purpose

   Expresses programmed gRNA scaffold from U6 promoter. Programmed gRNA scaffold for streamlined scRNA-seq in CRISPR screen (Direct-seq). 3rd generation lentiviral backbone.

   Depositor

   Lijia Ma

   Article

   Song et al Genome Biol. 2020 Jun 8;21(1):136. doi:

   Insert

   S. pyogenes sgRNA cassette (Loop2-8A8G)
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   hU6

   Available Since

   May 10, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pBabe-TetCMV-puro-mVenus-PA-Rac1

   Plasmid

   #22008

   Purpose

   Mammalian expression of photoactivatable Rac1

   Depositor

   Klaus Hahn

   Article

   Wu et al Nature. 2009 Sep 3. 461(7260):104-8.

   Insert

   PA-Rac1 (RAC1 Avena sativa (oat), Human)
   

   Use

   Retroviral

   Tags

   mVenus

   Expression

   Mammalian

   Mutation

   LOV(Leu404-Leu546), Rac1 starts at I4 and contain…

   Available Since

   Sept. 29, 2009

   Availability

   Academic Institutions and Nonprofits only

5. GG-EBNA-EEA-guide1-PGK-Puro

   Plasmid

   #102904

   Purpose

   EBNA episome plasmid for U6 promoter-driven expression of a gRNA targeting EEA-motif. Includes PGK-puro selection cassette.

   Depositor

   Timo Otonkoski

   Article

   Weltner et al Nat Commun. 2018 Jul 6;9(1):2643. doi: 1

   Insert

   EEA-guide1-PGK-Puro
   

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   July 10, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pBabe puro H-Ras G12V E37G

   Plasmid

   #12275

   Depositor

   Bob Weinberg

   Article

   Rangarajan et al Cancer Cell. 2004 Aug . 6(2):171-83.

   Insert

   H-Ras G12V E37G (HRAS Human)
   

   Use

   Retroviral

   Expression

   Mammalian

   Mutation

   Contains G12V activating mutation. The E37G mutat…

   Available Since

   July 20, 2006

   Availability

   Academic Institutions and Nonprofits only

7. Lv EF1a HP1cs-Frb2x PGK Puro

   Plasmid

   #102809

   Purpose

   Lentiviral plasmid from FIRE-Cas9 system to express HP1cs-Frb2x fusion protein

   Depositor

   Jerry Crabtree

   Article

   Braun et al Nat Commun. 2017 Sep 15;8(1):560. doi: 1

   Insert

   HP1cs-Frb2x
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   EF1a

   Available Since

   Feb. 9, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLenti-CMV-PtenK13E/K289E-Puro

   Plasmid

   #135681

   Purpose

   Constitutive expression (cMV promoter) of K13E/K289E mutant Pten cDNA, Puromycin selection

   Depositor

   Florian Karreth

   Insert

   Pten (Pten Mouse)
   

   Use

   Lentiviral

   Mutation

   K13E/K289E

   Available Since

   March 15, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pLEX-FLAG-NRAS-IRES-Puro

   Plasmid

   #120574

   Purpose

   Expresses Flag-N-Ras wildtype fusion protein in mammalian cells & for virus production

   Depositor

   Paul Khavari

   Article

   Kovalski et al Mol Cell. 2019 Jan 4. pii: S1097-2765(18

   Insert

   NRAS (NRAS Human)
   

   Use

   Lentiviral

   Tags

   FLAG

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Jan. 29, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pLKO.1-puro U6 sgRNA Oct4A -158

    Plasmid

    #50922

    Purpose

    U6 driven sgRNA targeting OCT4 isoform A -158 bp from TSS

    Depositor

    Rene Maehr, Scot Wolfe

    Article

    Kearns et al Development. 2014 Jan;141(1):219-23. doi

    Insert

    Oct4A -158 sgRNA (POU5F1 Human)
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Jan. 22, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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11. pLentiPGK Puro DEST JNKKTR AE Clover

    Plasmid

    #90240

    Purpose

    Lentiviral vector to express JNK KTR AE (mutant) mClover under PGK promoter (With Puromycin Resistance)

    Depositor

    Markus Covert

    Article

    Kudo et al Nat Protoc. 2018 Jan;13(1):155-169. doi:

    Insert

    JNK Kinase Translocation Reporter (AE mutant)
    

    Use

    Lentiviral

    Tags

    mClover

    Expression

    Mammalian

    Promoter

    PGK

    Available Since

    March 30, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pPuroR-MCS-LexA-VP16-MiniWhite

    Plasmid

    #165898

    Purpose

    Puromycin resistant LexA driver vector with Mini-white CDS eye marker. Contains an enhancer grammar GB20 entry point for custom enhancers. Standard cut-and-paste cloning can also be used. Vector uses Purple-White bacteria colony screening for identification of correct cloning

    Depositor

    Koen Venken

    Article

    Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi

    Insert

    Selectable Empty Enhancer Driver
    

    Use

    Synthetic Biology

    Available Since

    Sept. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pRetro-XT-PAK2-CFP(puro)-L107F

    Plasmid

    #81180

    Purpose

    Retroviral vector for expression of kinase active PAK2 with a CFP fusion

    Depositor

    Angeliki Malliri

    Article

    Whalley et al Nat Commun. 2015 Jun 16;6:7437. doi: 10.

    Insert

    p21 (RAC1) activated kinase 2 (PAK2 Human)
    

    Use

    Retroviral

    Tags

    CFP

    Mutation

    L107F

    Promoter

    P-tight TRE

    Available Since

    Aug. 9, 2018

    Availability

    Academic Institutions and Nonprofits only

14. pBabe-puro-miR-10b sponge

    Plasmid

    #25043

    Depositor

    Bob Weinberg

    Article

    Ma et al Nat Biotechnol. 2010 Apr . 28(4):341-7.

    Insert

    miR-10b sponge
    

    Use

    Retroviral

    Expression

    Mammalian

    Available Since

    June 11, 2010

    Availability

    Academic Institutions and Nonprofits only

15. Cenp-B-targeted Aurora B FRET sensor

    Plasmid

    #45232

    Depositor

    Michael Lampson

    Article

    Liu et al Science. 2009 Mar 6;323(5919):1350-3. do

    Insert

    CENP-B targeted Aurora B FRET sensor
    

    Expression

    Mammalian

    Promoter

    t7

    Available Since

    May 21, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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16. pIVTR(T7A)-Cas9n-RT-T2A-PuroR

    Plasmid

    #172296

    Purpose

    In vitro transcription of Cas9n-RT-T2A-PuroR synthetic construct for PRIME editing

    Depositor

    Patrik Verstreken

    Insert

    Cas9n-RT-T2A-PuroR
    

    Use

    CRISPR

    Mutation

    H840A (Cas9)

    Promoter

    T7 promoter A-initiating

    Available Since

    Sept. 3, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. LentiU6-hMYOD1 gRNA_multi1-3-MS2-Puro

    Plasmid

    #192683

    Purpose

    Lentiviral expression of multi gNAs targeting hMYOD1 promoter to activate human MYOD1 transcription

    Depositor

    Jeffrey Miner

    Article

    Omachi et al PLoS One. 2022 Jun 28;17(6):e0270008. do

    Insert

    Human MYOD1 activating gRNAs #1,2,3 (MYOD1 Human)
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Jan. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. attB-mCh-Dnmt3b1-Poly(A)-PuroR

    Plasmid

    #65556

    Purpose

    Plasmid for Bxb1-mediated recombination of the mCh-Dnmt3b1 cDNA into a MIN-tagged locus using Puromycin selection

    Depositor

    Sebastian Bultmann

    Article

    Mulholland et al Nucleic Acids Res. 2015 May 24. pii: gkv

    Insert

    Dnmt3b (Dnmt3b Mouse)
    

    Use

    Mouse Targeting; Bxb1

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    isoform 1

    Available Since

    Aug. 17, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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19. 970 pBabe puroL PTEN R130G

    Plasmid

    #10783

    Depositor

    William Sellers

    Insert

    PTEN (PTEN Human)
    

    Use

    Retroviral

    Expression

    Mammalian

    Mutation

    R130G

    Available Since

    Sept. 27, 2005

    Availability

    Academic Institutions and Nonprofits only

20. pSUPER retro puro macroH2A1 shRNA

    Plasmid

    #30517

    Depositor

    John Gurdon

    Article

    Pasque et al EMBO J. 2011 May 6;30(12):2373-87. doi:

    Insert

    mH2A1 shRNA
    

    Use

    RNAi and Retroviral

    Expression

    Mammalian

    Available Since

    Jan. 27, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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