We narrowed to 8,402 results for: 221
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Plasmid#80808Purposenuclear localization signal fused to N-terminus of GFP (nlsGFP) without stop codon, followed by P2ADepositorInsertminimal cytomegalovirus immediate early enhancer/promoter
Available SinceNov. 1, 2016AvailabilityAcademic Institutions and Nonprofits only -
pME-H2B-mCerulean3
Plasmid#75156PurposeNuclear mCerulean2 (blue) fluorophore for use with 5’ promoters.DepositorInsertH2B-mCerulean3
UseMiddle entry vector for multisite gateway three f…Promotern/aAvailable SinceOct. 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pENTR-PpU6P-sgRNA-L1L2
Plasmid#113735PurposeGateway entry vector containing attL1 and attL2 sites, U6 promoter, BsaI sites for protospacer ligation, and sgRNA.DepositorInsertPpU6P::sgRNA
UseCRISPR; Gateway entry vectorPromoterP. patens U6 promoterAvailable SinceSept. 24, 2018AvailabilityAcademic Institutions and Nonprofits only -
LLhG-pHG165a E62K
Plasmid#90051PurposeConstitutive expression of a chimeric LacI:GalR transcription repressorDepositorInsertLLhG/E62K
ExpressionBacterialMutationE62KPromoterIqAvailable SinceJune 23, 2017AvailabilityAcademic Institutions and Nonprofits only -
pENTR-PpU6P-sgRNA-L5L2
Plasmid#113738PurposeGateway entry vector containing attL5 and attL2 sites, U6 promoter, BsaI sites for protospacer ligation, and sgRNA.DepositorInsertPpU6P::sgRNA
UseCRISPR; Gateway entry vectorPromoterP. patens U6 promoterAvailable SinceSept. 24, 2018AvailabilityAcademic Institutions and Nonprofits only -
IF101: pMVP (L1-L4) EF1a promoter
Plasmid#121687PurposepMVP L1-L4 entry plasmid, contains EF1a promoter for 3-component MultiSite Gateway Pro assemblyDepositorInsertEF1a promoter
UseSynthetic Biology; Pmvp gateway entry plasmidAvailable SinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pENTR-PpU6P-sgRNA-R4R3
Plasmid#113739PurposeGateway entry vector containing attR4 and attR3 sites, U6 promoter, BsaI sites for protospacer ligation, and sgRNA.DepositorInsertPpU6P::sgRNA
UseCRISPR; Gateway entry vectorPromoterP. patens U6 promoterAvailable SinceSept. 24, 2018AvailabilityAcademic Institutions and Nonprofits only -
pENTR-PpU6P-sgRNA-L5L4
Plasmid#113741PurposeGateway entry vector containing attL5 and attL4 sites, U6 promoter, BsaI sites for protospacer ligation, and sgRNA.DepositorInsertPpU6P::sgRNA
UseCRISPR; Gateway entry vectorPromoterP. patens U6 promoterAvailable SinceSept. 24, 2018AvailabilityAcademic Institutions and Nonprofits only -
pENTR-PpU6P-sgRNA-L1R5
Plasmid#113737PurposeGateway entry vector containing attL1 and attR5 sites, U6 promoter, BsaI sites for protospacer ligation, and sgRNA.DepositorInsertPpU6P::sgRNA
UseCRISPR; Gateway entry vectorPromoterP. patens U6 promoterAvailable SinceSept. 24, 2018AvailabilityAcademic Institutions and Nonprofits only -
pENTR4_PRKRA
Plasmid#106110PurposeEntry vector for PRKRADepositorInsertPRKRA (PRKRA Human)
UseGateway entry vectorAvailable SinceFeb. 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
HB401: pMVP (L1-L4) CMV promoter
Plasmid#121686PurposepMVP L1-L4 entry plasmid, contains CMV promoter for 3-component MultiSite Gateway Pro assemblyDepositorInsertCMV promoter
UseSynthetic Biology; Pmvp gateway entry plasmidAvailable SinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
p5E-6xgata2aECEbasp
Plasmid#132968Purposegata2a endothelial enhancer (x6) and basal promoter in p5E Gateway vectorDepositorInsertgata2a endothelial enhancer (x6) with a b-actin basal promoter
UseGateway vectorAvailable SinceDec. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCFJ2359 - intron GG2 - 250bp
Plasmid#159878PurposeGolden Gate compatible intron with PATCs for reduced germline silencingDepositorInsertintron GG2 - 250bp
ExpressionWormMutationNot applicableAvailable SinceDec. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCFJ1149 - intron 3 smu-2 PATCs
Plasmid#161516PurposePATC-rich intron number three from smu-2 to stimulate germline expression in C. elegansDepositorInsertIntron 3 from smu-2
ExpressionWormAvailable SinceDec. 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
PKAcs-GGS4X-GFP
Plasmid#108583PurposeExpresses PKAcs-GFP fusion in mammalian cells with flexible linkerDepositorInsertprotein kinase A, catalytic subunit
TagsGFPExpressionMammalianMutationH87Q and W196RAvailable SinceFeb. 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-beta-hRLuc-V5-6xbsMS2-MALAT_X
Plasmid#147924PurposeMammalian Expression of 6xbsMS2-MalatDepositorInsert6xbsMS2-Malat (Malat1 Mouse)
ExpressionMammalianAvailable SinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
2X_pX458_pSpCas9(BB)-2A-GFP_SOX17_bKO
Plasmid#172225PurposeCas9-2A-GFP expression vector bearing two sgRNAs targeting the centromeric human SOX17 CTCF-boundary.DepositorInsertsgRNA
UseCRISPRTags3XFLAG and GFPExpressionMammalianPromoterCbhAvailable SinceSept. 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCFJ2370 - intron GG3 - 250bp
Plasmid#159879PurposeGolden Gate compatible intron with PATCs for reduced germline silencingDepositorInsertintron GG3 - 250bp
ExpressionWormMutationNot applicableAvailable SinceDec. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
VV209: sdChR(C138S E154A)-TS-GCaMP6f-ER in fck
Plasmid#58524PurposeExpresses sdChR(C138S E154A) fused to GCaMP6f in mammalian cellsDepositorInsertsdChR(C138S, E154A)-TS-GCaMP6f-ER
UseLentiviralTagsGCaMP6fExpressionMammalianMutationchanged Cysteine 138 to Serine; changed Glutamate…Promotera-CamKIIAvailable SinceNov. 5, 2014AvailabilityAcademic Institutions and Nonprofits only -
pENTR-Casp2
Plasmid#50023PurposepENTR vector for mouse Caspase-2DepositorInsertCasp2 (Casp2 Mouse)
UseEntry vectorAvailable SinceDec. 10, 2014AvailabilityAcademic Institutions and Nonprofits only -
pRN3P_T3_BE3_IVT
Plasmid#177015PurposePlasmid to be used as DNA template for in-vitro RNA transcription of the BE3 base editor (C to T) by T3 RNA polymeraseDepositorInsertBE3
UseCRISPR; Vector for in-vitro transcriptionPromoterT3 promoterAvailable SinceDec. 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
pENTR-mOvol1
Plasmid#120359PurposeGateway entry vector containing mouse Ovol1.DepositorAvailable SinceApril 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
pRS403red-luxTA2-4F
Plasmid#166030PurposeExpresses luxTA(2-4F) mutantDepositorInsertluxR
UseSynthetic BiologyTagsNuclear localization signal and three copies of V…ExpressionYeastMutationS116Y, W201R, codon-optimized for expression in y…PromoterpPGK1 mutantAvailable SinceMarch 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
pRS405red-phlTA2-1E
Plasmid#166029PurposeExpresses phlTA(2-1E) mutantDepositorInsertphlF
UseSynthetic BiologyTagsNuclear localization signal and three copies of V…ExpressionYeastMutationP5S, S6P, K86T, F109L, Q117R, E143K, codon-optimi…PromoterpPGK1 mutantAvailable SinceMarch 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGK415-luxTA2-4F
Plasmid#165971PurposeExpresses luxTA(2-4F) mutantDepositorInsertluxR
UseSynthetic BiologyTagsNuclear localization signal and three copies of V…ExpressionYeastMutationS116Y, W201R, codon-optimized for expression in y…PromoterpPGK1 mutantAvailable SinceMarch 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
pME-mKOFP2-CAAX
Plasmid#75155PurposeMembrane targeted KOFP2 (orange) fluorophore for use with 5’ promoters.DepositorInsertmKOFP2-CAAX
UseMiddle entry vector for multisite gateway three f…Promotern/aAvailable SinceOct. 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pFOSGE_COF4A02
Plasmid#71021PurposeGateway entry cloneDepositorInsertUbe3a (Ube3a Fly)
UseGateway entry vectorAvailable SinceDec. 1, 2015AvailabilityAcademic Institutions and Nonprofits only -
p5E-6xgata2aECE
Plasmid#132969Purposegata2a endothelial enhancer (x6) in p5E Gateway vectorDepositorInsertgata2a endothelial enhancer (x6)
UseGateway vectorAvailable SinceDec. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
IO001: pMVP (R4-R3) eGFP
Plasmid#121730PurposepMVP R4-R3 entry plasmid, contains eGFP for 3- or 4-component MultiSite Gateway Pro assemblyDepositorInserteGFP
UseSynthetic Biology; Pmvp gateway entry plasmidAvailable SinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
IG301: pMVP (L1-R5) CMV promoter
Plasmid#121692PurposepMVP L1-R5 entry plasmid, contains CMV promoter for 4-component MultiSite Gateway Pro assemblyDepositorInsertCMV promoter
UseSynthetic Biology; Pmvp gateway entry plasmidAvailable SinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
2X_pX458_pSpCas9(BB)-2A-GFP_NANOG_bKO
Plasmid#172224PurposeCas9-2A-GFP expression vector bearing two sgRNAs targeting the telomeric human NANOG CTCF-boundary.DepositorInsertsgRNA
UseCRISPRTags3XFLAG and GFPExpressionMammalianPromoterCbhAvailable SinceSept. 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
pME-QUAS5x:GFPNLS-SV40pA
Plasmid#155115PurposeMiddle entry vector containing QUAS5x element upstream of GFP-NLS.DepositorInsertQUAS5x:GFPNLS
UseGateway middle entry vectorPromoterQUAS5x-E1bAvailable SinceSept. 14, 2020AvailabilityAcademic Institutions and Nonprofits only -
pENTR-R4R3-3xmEGFP-C
Plasmid#113751PurposeGateway multi-site entry clone for second position 3x mEGFP (C-terminal tag). Introduced into the genome via homology-directed repair after an LR reaction with homology sequences.DepositorInsertmEGFP-mEGFP-mEGFP
UseCRISPR; Gateway entry vectorTags3xmEGFPAvailable SinceSept. 24, 2018AvailabilityAcademic Institutions and Nonprofits only -
IO201: pMVP (R4-R3) mCherry
Plasmid#121732PurposepMVP R4-R3 entry plasmid, contains mCherry for 3- or 4-component MultiSite Gateway Pro assemblyDepositorInsertmCherry
UseSynthetic Biology; Pmvp gateway entry plasmidAvailable SinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCFJ66
Plasmid#24981DepositorInsertCbr-unc-119 Punc-122::GFP
UseMultisite gatewayAvailable SinceAug. 4, 2010AvailabilityAcademic Institutions and Nonprofits only -
JZ901: pMVP (L5-L4) EF1a promoter
Plasmid#121727PurposepMVP L5-L4 entry plasmid, contains EF1a promoter for 4-component MultiSite Gateway Pro assemblyDepositorInsertEF1a promoter
UseSynthetic Biology; Pmvp gateway entry plasmidAvailable SinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
sspa-GCaMP6s
Plasmid#67559Purposeshort photoactivatable fluorescent calcium reporterDepositorInsertspa-GCaMP6s
ExpressionMammalianMutationK65T, I83V, G87S, Y92D, V115H, K118V, S187R, Y196…Available SinceJuly 28, 2015AvailabilityAcademic Institutions and Nonprofits only -
IG001: pMVP (L1-L4) TBG promoter
Plasmid#121689PurposepMVP L1-L4 entry plasmid, contains TBG promoter for 3-component MultiSite Gateway Pro assemblyDepositorInsertTBG promoter (Liver specific)
UseSynthetic Biology; Pmvp gateway entry plasmidAvailable SinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
MSCV-ERK5-AEF
Plasmid#31816DepositorInsertErk5 (Mapk7 Mouse)
UseRetroviralExpressionMammalianMutationT219A and Y221FPromoterPCMV-LTRAvailable SinceSept. 20, 2011AvailabilityAcademic Institutions and Nonprofits only -
pGK415-phlTA2-1E
Plasmid#165970PurposeExpresses phlTA(2-1E) mutantDepositorInsertphlF
UseSynthetic BiologyTagsNuclear localization signal and three copies of V…ExpressionYeastMutationP5S, S6P, K86T, F109L, Q117R, E143K, codon-optimi…PromoterpPGK1 mutantAvailable SinceMarch 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
pENTR-R4R3-3xmRuby-N
Plasmid#113754PurposeGateway multi-site entry clone for second position 3x mRuby (N-terminal tag). Introduced into the genome via homology-directed repair after an LR reaction with homology sequences.DepositorInsertmRuby2-mRuby2-mRuby2
UseCRISPR; Gateway entry vectorTags3xmRuby2Available SinceSept. 24, 2018AvailabilityAcademic Institutions and Nonprofits only -
KJ101: pMVP (R4-R3) osTir1
Plasmid#121742PurposepMVP R4-R3 entry plasmid, contains osTir1 for 3- or 4-component MultiSite Gateway Pro assemblyDepositorInsertosTir1
UseSynthetic Biology; Pmvp gateway entry plasmidTags9x myc epitope tagAvailable SinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
IG401: pMVP (L1-R5) RIP
Plasmid#121694PurposepMVP L1-R5 entry plasmid, contains rat Ins1 promoter (RIP) for 4-component MultiSite Gateway Pro assemblyDepositorInsertRIP
UseSynthetic Biology; Pmvp gateway entry plasmidAvailable SinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
IG603: pMVP (L1-L4) TRE promoter
Plasmid#121691PurposepMVP L1-L4 entry plasmid, contains TRE promoter for 3-component MultiSite Gateway Pro assemblyDepositorInsertTRE promoter
UseSynthetic Biology; Pmvp gateway entry plasmidAvailable SinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
IG101: pMVP (L5-L4) TRE promoter
Plasmid#121729PurposepMVP L5-L4 entry plasmid, contains TRE promoter for 4-component MultiSite Gateway Pro assemblyDepositorInsertTRE promoter
UseSynthetic Biology; Pmvp gateway entry plasmidAvailable SinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
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pET28-his-3C_Clu3B6p
Plasmid#100093Purposeexpresses easily crystallizable engineered 14-3-3 sigma variant fused to the HSPB6 phosphorylatable peptideDepositorInsert14-3-3 sigma human (SFN Human)
TagsHSPB6 peptide and His6ExpressionBacterialMutation75-77 residues changed to AAA to facilitate cryst…PromoterT7Available SinceSept. 22, 2017AvailabilityAcademic Institutions and Nonprofits only -
pUPD2-ODPBMTS (12)
Plasmid#101695PurposeODPBMTS in domestication vectorDepositorInsertCCAT-[MTS from S. cerevisiae Pyruvate dehydrogenase E1 component subunit beta (YBR221C)]-AATG
ExpressionYeastAvailable SinceJuly 24, 2018AvailabilityAcademic Institutions and Nonprofits only -
VV228: sdChR(C138S E154A)-TS in fck
Plasmid#58523PurposeExpresses sdChR(C138S E154A) in mammalian cellsDepositorInsertsdChR(C138S, E154A)-TS
UseLentiviralExpressionMammalianMutationchanged Cysteine 138 to Serine; changed Glutamate…Promotera-CamKIIAvailable SinceNov. 5, 2014AvailabilityAcademic Institutions and Nonprofits only -
pENTR-R4R3-mEGFP-N
Plasmid#113746PurposeGateway multi-site entry clone for second position mEGFP (N-terminal tag). Introduced into the genome via homology-directed repair after an LR reaction with homology sequences.DepositorInsertmEGFP
UseCRISPR; Gateway entry vectorTagsmEGFPAvailable SinceSept. 24, 2018AvailabilityAcademic Institutions and Nonprofits only