We narrowed to 44,597 results for: cas

1. pET21b-Casp1-His

   Plasmid

   #11809

   Depositor

   Guy Salvesen

   Insert

   Caspase-1 (CASP1 Human)
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   May 12, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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2. Tubp-Gal80 in pCaSpeR4

   Plasmid

   #17748

   Depositor

   Liqun Luo

   Article

   Lee et al Neuron. 1999 Mar . 22(3):451-61.

   Insert

   GAL80 (GAL80 Budding Yeast)
   

   Use

   Tags

   SV40 polyA and Tubulin 1alpha promoter

   Expression

   Insect

   Mutation

   Promoter

   Available since

   May 14, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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3. RCAS-Flag-BRAFV600E

   Plasmid

   #126615

   Purpose

   Retroviral expression of Flag tagged human oncogenic BRAFV600E

   Depositor

   Yi Li

   Article

   Bu et al Cancer Res. 2019 Jan 1;79(1):61-71. doi:

   Insert

   BRAF (BRAF Human)
   

   Use

   Retroviral

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   V600E

   Promoter

   Available since

   July 23, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pdCas9::BFP-humanized

   Plasmid

   #44247

   Purpose

   Expression of a catalytically inactive, human codon-optimized Cas9-BFP fusion under the control of Murine Stem Cell retroVirus LTR promoter for mammalian gene knockdown

   Depositor

   Stanley Qi

   Article

   Qi et al Cell. 2013 Feb 28;152(5):1173-83. doi: 1

   Insert

   dCas9 fused to BFP
   

   Use

   CRISPR and Retroviral

   Tags

   2xNLS & tagBFP

   Expression

   Mammalian

   Mutation

   D10A H840A (catalytically inactive)

   Promoter

   MSCV LTR promoter

   Available since

   April 8, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLV_dCas9-ZIM3-KRAB_sgTAOK2i_#3

   Plasmid

   #172985

   Purpose

   CRISPRi for TAOK2

   Depositor

   Michael Wehr

   Article

   Ma et al Cell Signal. 2024 Jan;113:110917. doi: 1

   Insert

   sgTAOK2i (TAOK2 Human)
   

   Use

   CRISPR and Lentiviral

   Tags

   3xFLAG

   Expression

   Mutation

   Promoter

   Available since

   Oct. 11, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. p-dCas9-TET1-Hygro

   Plasmid

   #104404

   Purpose

   transient expression of dCas9-TET1 fusion protein

   Depositor

   Stephan Beck

   Insert

   TET1 (TET1 Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV promoter

   Available since

   April 2, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. AW01_pU.CAG.eSpCas9(1.1).rBGpA

   Plasmid

   #199255

   Purpose

   Expression construct encoding the high specificity eSpCas9(1.1) nuclease

   Depositor

   Manuel Goncalves

   Article

   Chen et al Nucleic Acids Res. 2016 Jun 8. pii: gkw5

   Insert

   High specificity eSpCas9(1.1) nuclease
   

   Use

   CRISPR

   Tags

   3XFLAG epitope, Nucleoplasmin NLS, and SV40 NLS

   Expression

   Mammalian

   Mutation

   K848A K1003A R1060A

   Promoter

   CAG promoter

   Available since

   April 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCold CL7-Cas12a

   Plasmid

   #124891

   Purpose

   obtaining full Cas12a RNP

   Depositor

   Yi Liu

   Article

   Qiao et al Commun Biol. 2019 May 3;2:161. doi: 10.1

   Insert

   Cas12a
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Feb. 14, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. p3s-Sniper-Cas9

   Plasmid

   #113912

   Purpose

   Expresses Sniper-Cas9 with CMV promoter

   Depositor

   Jungjoon Lee

   Article

   Lee et al Nat Commun. 2018 Aug 6;9(1):3048. doi: 1

   Insert

   Sniper-Cas9
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Aug. 8, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pcDNA3-Casp8 C360A

    Plasmid

    #11818

    Depositor

    Guy Salvesen

    Article

    Stennicke et al J Biol Chem. 1997 Oct 10. 272(41):25719-

    Insert

    Caspase 8 catalytic mutant (CASP8 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    C360A

    Promoter

    Available since

    May 12, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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11. pET-His6-FnCas9GFP

    Plasmid

    #130966

    Purpose

    expression of FnCas9-GFP in bacterial cells

    Depositor

    Debojyoti Chakraborty

    Article

    Acharya et al Proc Natl Acad Sci U S A. 2019 Oct 15;11

    Insert

    HA-NLS-FnCas9
    

    Use

    CRISPR

    Tags

    6xHis-mEGFP-TEV-HA-NLS

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Nov. 7, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pEM-Cas9HF1-indRecA56

    Plasmid

    #102294

    Purpose

    Modified from pEM-Cas9HF1 (Addgene ID: 89961) to co-express inducible recA56 to block recA-mediated double-strand break repair.

    Depositor

    Michael Lynch

    Article

    Moreb et al ACS Synth Biol. 2017 Oct 2. doi: 10.1021

    Inserts

    Cas9HF1
    recA56
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    N497A/R661A/Q695A/Q926A

    Promoter

    pTet

    Available since

    April 12, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pX330-SpCas9-HF1

    Plasmid

    #108301

    Purpose

    pX330 (Plasmid #42230) with the N497A, R661A, Q695A, and Q926A mutations

    Depositor

    Yuichiro Miyaoka

    Article

    Kato-Inui et al Nucleic Acids Res. 2018 May 18;46(9):467

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. RCASBP-Y NHA-EGFP

    Plasmid

    #11481

    Depositor

    William Pavan

    Article

    Loftus et al DNA Res. 2001 Oct 31. 8(5):221-6.

    Insert

    Enhanced Green Fluoresecent Protein
    

    Use

    Retroviral

    Tags

    HA

    Expression

    Mutation

    Promoter

    Available since

    March 22, 2006

    Availability

    Academic Institutions and Nonprofits only

15. V2-MESA-35F-M-dCas9

    Plasmid

    #84506

    Purpose

    MESA target chain with V2-MESA ectodomain, 35 extracellular linkers, a flag tag, M cleavage sequence, and dCas9-VP64

    Depositor

    Joshua Leonard

    Article

    Schwarz et al Nat Chem Biol. 2016 Dec 12. doi: 10.1038

    Insert

    V2-MESA-35F-M-dCas9
    

    Use

    Lentiviral

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 11, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. 3xFLAG-dCas9/pMSCVhyg

    Plasmid

    #134323

    Purpose

    Expresses 3xFLAG-dCas9 in mammalian cells for enChIP analysis to purify specific genomic regions of interest.

    Depositor

    Hodaka Fujii

    Article

    Yuno et al Biol Methods Protoc. 2021 Jul 9;6(1):bpa

    Insert

    3xFLAG-dCas9
    

    Use

    CRISPR and Retroviral

    Tags

    3xFLAG tag and NLS

    Expression

    Mammalian

    Mutation

    human codon-optimized, D10A + H840A

    Promoter

    LTR

    Available since

    April 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. IVT-nCas9(H840A)

    Plasmid

    #207455

    Purpose

    in vitro transcription of mRNA

    Depositor

    Erik Sontheimer

    Article

    Liu et al Nat Biotechnol. 2023 Sep 14. doi: 10.103

    Insert

    H840A
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Nov. 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. eSpCas9(1.1)_NoFlag_hROSA26_Left

    Plasmid

    #191442

    Purpose

    Expresses the hROSA26 left sgRNA in combination with FLAGless eSpCas9(1.1) to target the hRosa26 safe harbor locus

    Depositor

    Yannick Doyon

    Insert

    hROSA26 sgRNA
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CBh

    Available since

    Feb. 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. pAAV.minBG-CI-SaCas9-W3SynpA

    Plasmid

    #231369

    Purpose

    minBG-driven SaCas9, with short synthetic terminator sequence.

    Depositor

    Viviana Gradinaru

    Article

    Coughlin et al Nat Biotechnol. 2025 Mar 20. doi: 10.103

    Insert

    SaCas9
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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20. pLenti-nSpCas9-NoABE

    Plasmid

    #209043

    Purpose

    Lentiviral vector expressing doxycycline-inducible human codon-optimized SpCas9(D10A)-6xNLS (Cas9 nickase)

    Depositor

    Thomas Gonatopoulos-Pournatzis

    Article

    Xiao et al Mol Cell. 2024 Jul 11;84(13):2553-2572.e

    Insert

    nSpCas9
    

    Use

    CRISPR and Lentiviral

    Tags

    FLAG-HA

    Expression

    Mammalian

    Mutation

    D10A

    Promoter

    Tight TRE promoter

    Available since

    June 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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