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164,388 results

Showing: 4111 - 4140 of 164388 results

  3. SpCas9-HF1

    Plasmid
    #138556
    Purpose
    Expresses human codon-optimized high-fidelity SpCas9 and blasticidin resistance: EFS promoter-SpCas9-HF1-NLS-FLAG-P2A-BSD
    Depositor
    Hyongbum Kim
    Article
    Kim et al Nat Biotechnol. 2020 Nov;38(11):1328-133
    Insert
    SpCas9-HF1
    Use
    CRISPR and Lentiviral
    Tags
    BSD, FLAG, and NLS
    Expression
    Mammalian
    Mutation
    N497A, R661A, Q695A, Q926A
    Promoter
    EFS
    Available since
    June 9, 2020
    Availability
    Academic Institutions and Nonprofits only
    138556-plasmid-map-sequence-id-274763

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  8. pAAV-CAG-tdTomato (codon diversified) (AAV PHP.eB)

    Viral Prep
    #59462-PHPeB
    Purpose
    Ready-to-use AAV PHP.eB particles produced from pAAV-CAG-tdTomato (codon diversified) (#59462). In addition to the viral particles, you will also receive purified pAAV-CAG-tdTomato (codon diversified) plasmid DNA. CAG-driven tdTomato expression control. These AAV were produced with the PHPeB serotype, which permits efficient transduction of the central nervous system. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Edward Boyden
    Promoter
    CAG
    Tags
    tdTomato (codon diversified)
    Available since
    Feb. 28, 2018
    Availability
    Academic Institutions and Nonprofits only

  10. pLenti.CAG.H2B-Dendra2.W

    Plasmid
    #51005
    Purpose
    Lentivector that expresses H2B-Dendra2 from internal CAG enhancer-promoter
    Depositor
    Rusty Lansford
    Insert
    H2B-Dendra2
    Use
    Lentiviral
    Tags
    histone2B
    Expression
    Mammalian
    Mutation
    Promoter
    CAG
    Available since
    June 2, 2014
    Availability
    Academic Institutions and Nonprofits only
    51005-plasmid-map-sequence-id-77767

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  16. pJRH-1345 U6-B2M sgRNA Gag-Cas9 v2

    Plasmid
    #201916
    Purpose
    Cas9-EDV production plasmid. Expresses the v2 Gag-Cas9 polypeptide (CAG promoter) and B2M sgRNA (human U6 promoter, 5’-GAGTAGCGCGAGCACAGCTA)
    Depositor
    Jennifer Doudna
    Article
    Hamilton et al Nat Biotechnol. 2024 Jan 11. doi: 10.103
    Inserts
    Gag-Cas9 v2
    B2M sgRNA (Target-specific sequence: 5'- GAGTAGCGCGAGCACAGCTA)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CAG and Human U6
    Available since
    Aug. 4, 2023
    Availability
    Academic Institutions and Nonprofits only
    201916-plasmid-map-sequence-id-403306

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  18. pBacMam2-DiEx-LIC-C-flag_huntingtin_full-length_Q109

    Plasmid
    #111730
    Purpose
    Baculovirus transfer vector for expression of huntingtin protein in insect and mammalian cellscells as well as in mammalian cells
    Depositor
    Cheryl Arrowsmith
    Article
    Harding et al J Biol Chem. 2019 Apr 26;294(17):6986-70
    Insert
    HTT (HTT Human)
    Use
    Baculovirus expression
    Tags
    FLAG
    Expression
    Mammalian
    Mutation
    Q109 (polyQ repeat)
    Promoter
    CMV and P10
    Available since
    Oct. 31, 2018
    Availability
    Academic Institutions and Nonprofits only
    111730-plasmid-map-sequence-id-226806

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  20. GFP-itis Bacterial Base Editor Activity

    Plasmid Kit
    #1000000219
    Purpose
    This collection contains all plasmids needed for preparation and execution of the "GFP-itis" bacterial base editing activity.
    Depositor
    Alexis Komor
    Available since
    May 1, 2023
    Availability
    Academic Institutions and Nonprofits only

  24. pCAGGS-PHFF-sh-hBVRA

    Plasmid
    #100285
    Purpose
    Expression vector for phycocyanobilin (PCB) synthesis and shRNA for human BVRA gene in mammalian cells
    Depositor
    Kazuhiro Aoki
    Article
    Uda et al Proc Natl Acad Sci U S A. 2017 Oct 24. p
    Inserts
    MTS-PcyA-FLAG-P2A-MTS-HA-HO1-P2A-MTS-Myc-Fd-P2A-MTS-Fnr-T7 (synthetic genes)
    shRNA for human BVRA
    Use
    Tags
    FLAG, T7 and Mitochondria-targeting sequence (MTS…
    Expression
    Mammalian
    Mutation
    All genes are codon-optimized for expression in h…
    Promoter
    CAG promoter and H1 promoter
    Available since
    Sept. 20, 2017
    Availability
    Academic Institutions and Nonprofits only
    100285-plasmid-map-sequence-id-194439

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  26. R777-E285 Hs.ROCK2

    Plasmid
    #70569
    Purpose
    Gateway ORF clone of human ROCK2 [NM_004850.3] with stop codon (for native or N-terminal fusions)
    Depositor
    Dominic Esposito
    Insert
    ROCK2 (ROCK2 Human)
    Use
    Gateway entry clone
    Tags
    Expression
    Mutation
    Promoter
    Available since
    March 1, 2016
    Availability
    Academic Institutions and Nonprofits only
    70569-plasmid-map-sequence-id-126018

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  27. pAAV-hSyn-dF-HA-KORD-IRES-mCitrine (AAV8)

    Viral Prep
    #65417-AAV8
    Purpose
    Ready-to-use AAV8 particles produced from pAAV-hSyn-dF-HA-KORD-IRES-mCitrine (#65417). In addition to the viral particles, you will also receive purified pAAV-hSyn-dF-HA-KORD-IRES-mCitrine plasmid DNA. hSyn-driven, Cre-dependent KORD expression with bicistronic mCitrine, for SALB-induced neuronal inhibition. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Bryan Roth
    Article
    Vardy et al Neuron. 2015 Apr 29. pii: S0896-6273(15)
    Promoter
    Syn
    Tags
    mCitrine
    Available since
    Oct. 26, 2017
    Availability
    Academic Institutions and Nonprofits only

  28. pHPHS232

    Plasmid
    #228356
    Purpose
    Landing pad with Bxb1 attP_wildtype site, Dox-inducible BFP-iCasp9-Blasticidin, CMV-driven rtTA3, T2A-neomycin for HDR into AAVS1 locus
    Depositor
    Arvind Rasi Subramaniam
    Article
    Nugent et al bioRxiv [Preprint]. 2024 Jul 26:2024.07.
    Inserts
    mTagBFP2
    Reverse tetracycline transactivator 3
    neo
    Use
    Tags
    Blasticidin S deaminase and iCasp9
    Expression
    Mammalian
    Mutation
    Promoter
    TRE3GV and gene trap
    Available since
    Jan. 2, 2025
    Availability
    Academic Institutions and Nonprofits only
    228356-plasmid-map-sequence-id-455149

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Showing: 4111 - 4140 of 164388 results