166,818 results
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Plasmid#52535Purposeenables eukaryotic expression of N-terminally FLAG-HA-tagged fusion proteinsDepositorHas ServiceCloning Grade DNATypeEmpty backboneUseTagsFLAG-HAExpressionMammalianMutationPromoterCMVAvailable SinceJuly 31, 2015AvailabilityAcademic Institutions and Nonprofits only
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CNR1-Tango
Plasmid#66254PurposeExpression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocationDepositorAvailable SinceSept. 15, 2015AvailabilityAcademic Institutions and Nonprofits only -
pC-KIT8
Plasmid#118990PurposeMutational dissection of the G rich region of the c-kit promoterDepositorInsertc-kit proto-oncogene {promoter} (KIT Human)
UseTagsExpressionMammalianMutationmutK2-mutSP-mutK1PromoterAvailable SinceJune 10, 2019AvailabilityAcademic Institutions and Nonprofits only -
EphA2-YFP
Plasmid#108852PurposeEncodes for human EphA2 fluorescently labeled with eYFP on the C-Terminus via a 15 amino acid (GGS)5 flexible linkerDepositorInsertEPHA2 (EPHA2 Human)
UseTagsLabeled with eYFP on the C-Terminus via a 15 amin…ExpressionMammalianMutationPromoterAvailable SinceApril 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
pFETCh_KLF9
Plasmid#86286PurposeDonor vector for 3' FLAG tag of human KLF9DepositorAvailable SinceFeb. 1, 2017AvailabilityAcademic Institutions and Nonprofits only -
PX458_KLF9_2
Plasmid#86347PurposeEncodes gRNA for 3' target of human KLF9DepositorAvailable SinceJan. 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
PX458_KLF9_1
Plasmid#86346PurposeEncodes gRNA for 3' target of human KLF9DepositorAvailable SinceJan. 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
AARS_C2_pNIC-Bio3
Plasmid#153047PurposeExpression construct for producing Avi tagged Alanyl-tRNA synthetaseDepositorAvailable SinceJune 29, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAAV_BiSSTe4_dTomato_nlsdTomato (AAV1)
Viral Prep#213944-AAV1PurposeReady-to-use AAV1 particles produced from pAAV_BiSSTe4_dTomato_nlsdTomato (#213944). In addition to the viral particles, you will also receive purified pAAV_BiSSTe4_dTomato_nlsdTomato plasmid DNA. Bicistronic expression of dTomato and nuclear-targeted dTomato under the control of the SST interneuron-targeting enhancer E4. These AAV preparations are suitable purity for injection into animals.DepositorPromoterTagsdTomatoAvailable SinceSept. 23, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAAV_BiPVe3_dTomato_nlsdTomato (AAV1)
Viral Prep#213940-AAV1PurposeReady-to-use AAV1 particles produced from pAAV_BiPVe3_dTomato_nlsdTomato (#213940). In addition to the viral particles, you will also receive purified pAAV_BiPVe3_dTomato_nlsdTomato plasmid DNA. Bicistronic expression of dTomato and nuclear-targeted dTomato under the control of the PV+ basket cell-targeting enhancer E3. These AAV preparations are suitable purity for injection into animals.DepositorPromoterTagsdTomatoAvailable SinceSept. 23, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAAV_BiPVe4_dTomato_nlsdTomato (AAV1)
Viral Prep#213936-AAV1PurposeReady-to-use AAV1 particles produced from pAAV_BiPVe4_dTomato_nlsdTomato (#213936). In addition to the viral particles, you will also receive purified pAAV_BiPVe4_dTomato_nlsdTomato plasmid DNA. Bicistronic expression of dTomato and nuclear-targeted dTomato under the control of the chandelier cell-targeting enhancer E4. These AAV preparations are suitable purity for injection into animals.DepositorPromoterTagsdTomatoAvailable SinceSept. 23, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAAV_BiSSTe10_dTomato_nlsdTomato (AAV1)
Viral Prep#213814-AAV1PurposeReady-to-use AAV1 particles produced from pAAV_BiSSTe10_dTomato_nlsdTomato (#213814). In addition to the viral particles, you will also receive purified pAAV_BiSSTe10_dTomato_nlsdTomato plasmid DNA. Bicistronic expression of dTomato and nuclear-targeted dTomato under the control of the SST interneuron-targeting enhancer E10. These AAV preparations are suitable purity for injection into animals.DepositorPromoterTagsdTomatoAvailable SinceSept. 23, 2025AvailabilityAcademic Institutions and Nonprofits only -
DH-BB[5'SIN;181/25ORF]
Plasmid#242479PurposeHelper plasmid to produce CHIKV-pseudotyped propogation incompetent Sindbis virusDepositorInsertChikungunya virus 181/25 strain structural proteins
UseSindbis virus helper constructTagsExpressionMutationPromoterAvailable SinceSept. 19, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CAG-FLEx-tdT-P2A-Mxra8
Plasmid#242478PurposeAAV vector expressing Mxra8 and tdTomato under cre-recombinase controlDepositorInsertsUseAAV and Cre/LoxTagsFLAGExpressionMammalianMutationPromoterAvailable SinceSept. 18, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CAG-tdT-P2A-Mxra8
Plasmid#242477PurposeAAV vector expressing Mxra8 and tdTomatoDepositorAvailable SinceSept. 18, 2025AvailabilityAcademic Institutions and Nonprofits only -
pEM3_Euk_2NLS-Flex-Cas12a (LbCas12a)-2NLS
Plasmid#244838PurposeMammalian expression of Flex-Cas12a with 2xSV40 NLS at N-terminus and 2xSV40 at C-terminusDepositorInsertFlex-Cas12a
UseCRISPRTags2xSV40 NLSExpressionMammalianMutationG146R/R182V/D535G/S551F/D665N/E795QPromoterT7Available SinceSept. 17, 2025AvailabilityAcademic Institutions and Nonprofits only -
Cp PS Intein tTA
Plasmid#242026PurposeBlue-light-controlled release of tTA transactivator from the cytoplasm to the nucleus.DepositorInsertCp PS Intein tTA
UseTagsExpressionMammalianMutationPromoterCMVAvailable SinceSept. 16, 2025AvailabilityAcademic Institutions and Nonprofits only -
pFS561
Plasmid#242861PurposeThis plasmid will be used to replace the promoter of a gene with the PenotetS promoter at its endogenous locusDepositorInsertsTetR
mECitrine
UseTagsExpressionYeastMutationPromoterCMV promoter and enotetS promoterAvailable SinceSept. 16, 2025AvailabilityAcademic Institutions and Nonprofits only -
IGF2BP3-HaloTag-HiBiT Fusion Vector
Plasmid#238631PurposeExpress IGF2BP3-HaloTag-HiBiT Fusion Protein in Mammalian Cells under a CMV promoterDepositorHas ServiceDNAInsertIGF2BP3 (IGF2BP3 Human)
UseTagsHaloTag (R)-HiBiTExpressionMammalianMutationNonePromoterCMVAvailable SinceSept. 14, 2025AvailabilityIndustry, Academic Institutions, and Nonprofits -
pFS562
Plasmid#242862PurposeThis plasmid will be used to replace the promoter of a gene with the PenotetSW1 promoter at its endogenous locusDepositorInsertsTetR
mECitrine
UseTagsExpressionYeastMutationPromoterCMV promoter and enotetSW1 promoterAvailable SinceSept. 10, 2025AvailabilityAcademic Institutions and Nonprofits only -
pFS564
Plasmid#242864PurposeThis plasmid will be used to replace the promoter of a gene with the PenotetSW3 promoter at its endogenous locusDepositorInsertsTetR
mECitrine
UseTagsExpressionYeastMutationPromoterCMV promoter and enotetSW3 promoterAvailable SinceSept. 10, 2025AvailabilityAcademic Institutions and Nonprofits only -
pFS565
Plasmid#242865PurposeThis plasmid will be used to replace the promoter of a gene with the PenotetSW4 promoter at its endogenous locusDepositorInsertsTetR
mECitrine
UseTagsExpressionYeastMutationPromoterCMV promoter and enotetSW4 promoterAvailable SinceAug. 25, 2025AvailabilityAcademic Institutions and Nonprofits only -
Lenti NG-ABE8e_P2A_GFP + PuroR
Plasmid#242000PurposeLentiviral plasmid for generation of cell lines stably expressing NG-ABE8e base editor. NG-ABE8e expression can be followed by GFP fluorescence. Contains PGK-puromycin resistance cassette.DepositorInsertNG-ABE8e
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterAvailable SinceAug. 25, 2025AvailabilityAcademic Institutions and Nonprofits only -
p131CB10-tphKAB
Plasmid#241911PurposeTPA catabolic operon under the control of a PCA inducible biosensorDepositorInserttphK, tphA2, tphA3, tphB, tphA1
UseSynthetic BiologyTagsExpressionMutationnonePromoterAvailable SinceAug. 25, 2025AvailabilityAcademic Institutions and Nonprofits only -
NanoLuc-CA2 Fusion Vector
Plasmid#237187PurposeExpress NanoLuc(R)-CA2 Fusion Protein in Mammalian Cells under a CMV promoterDepositorHas ServiceDNAInsertCA2 (CA2 Human)
UseTagsNanoLuc (R)ExpressionMammalianMutationPromoterCMVAvailable SinceJuly 14, 2025AvailabilityIndustry, Academic Institutions, and Nonprofits -
pNL [NlucP/STAT6-RE/Hygro] Vector
Plasmid#236925PurposeExpress STAT6 response element in luciferase reporter assayDepositorHas ServiceDNAInsertSTAT6 (STAT6 Human)
UseLuciferaseTagsNanoLuc (R)ExpressionMutationNonePromoterminPAvailable SinceJuly 14, 2025AvailabilityIndustry, Academic Institutions, and Nonprofits -
pNL [NlucP/SIE/Hygro] Vector
Plasmid#236833PurposeExpress SIE response element in luciferase reporter assayDepositorHas ServiceDNAInsertSIE
UseLuciferaseTagsNanoLuc (R)ExpressionMutationNonePromoterminPAvailable SinceJuly 14, 2025AvailabilityIndustry, Academic Institutions, and Nonprofits -
pETM11-6xHis-TEV-hORF1p
Plasmid#202589PurposeAllows for IPTG-inducible expression of the human L1RP ORF1 protein in bacteria with an N-terminal 6xHis tag and TEV cleavage site for purification with a minimal scarDepositorInsertORF1p
UseTags6x His and TEV protease cleavage siteExpressionBacterialMutationPromoterAvailable SinceJune 4, 2025AvailabilityAcademic Institutions and Nonprofits only -
pRS413-TEF1pr-MFA-NFAPoptim
Plasmid#221095PurposeFAP insert for N-terminally tagging genes of interest (optimized FAP for yeast expression + 2xMYC tag) + MFA1 signal sequence to help target constructs to the ERDepositorTypeEmpty backboneUseTagsExpressionYeastMutationPromoterAvailable SinceApril 3, 2025AvailabilityAcademic Institutions and Nonprofits only -
TFORF2325
Plasmid#144011PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorAvailable SinceDec. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
Ydf NanoLuc
Plasmid#225656PurposeReporter plasmid expressing NanoLuciferase under the control of the promoter PydfJ115, cognate to YdfI. Responds to NarL/NarX chimeras from this study.DepositorInsertNanoLuciferase
UseCell free expressionTagsMycExpressionBacterialMutationPromoterPydfJ115Available SinceDec. 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
pJL NarL YdfI Flag
Plasmid#225650PurposeExpresses NarL with a mutated DNA binding domain (YdfI). For use with NarX chimeras and reporter plasmids from this study.DepositorInsertNarL-YdfI (narL Synthetic)
UseCell free expressionTagsFLAGExpressionBacterialMutationNarL with native DNA-binding domain replaced with…PromoterT7Available SinceDec. 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
pJL NarX Myc
Plasmid#225651PurposeExpresses WT NarX capable of sensing nitrate. For use with NarL-YdfI and reporter plasmids from this study.DepositorInsertNarX (narX E. coli)
UseCell free expressionTagsMycExpressionBacterialMutationPromoterT7Available SinceNov. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
TL1 DdCBE A1 (DddA6-C)
Plasmid#221992PurposeExpresses mitochondrial cytosine base editor arm in mammalian cellsDepositorInsertCOX8A MTS-3xFLAG-TALE NT-T-TL1 TALE A1 repeats-TALE CTD-DddA6/11 1397C-UGI-ATP5B 3'UTR
UseCRISPRTags3xFLAGExpressionMammalianMutationDddA: T1413IPromoterCMVAvailable SinceSept. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
TL1 DdCBE T2 (DddA6-N)
Plasmid#221993PurposeExpresses mitochondrial cytosine base editor arm in mammalian cellsDepositorInsertCOX8A MTS-3xFLAG-TALE NT-T-TL1 TALE T2 repeats-TALE CTD-DddA6 1397N-UGI-ATP5B 3'UTR
UseCRISPRTags3xFLAGExpressionMammalianMutationDddA: Q1310R, S1330I, T1380IPromoterCMVAvailable SinceSept. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
2C-EGFP-t2a-mCD4
Plasmid#216213PurposeExpresses GFP and mCD4 transmembrane domain under control of the 2C-specific MERVL promoterDepositorInsertCD4 (Cd4 Mouse)
UseTagsGFP-T2AExpressionMammalianMutationCD4 transmembrane domain onlyPromoterMERVLAvailable SinceMay 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
EGFP-Cyclin D3
Plasmid#215052PurposeExpresses EGFP-Cyclin D3 in mammalian cells from a retroviral vector.DepositorAvailable SinceApril 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
mU6-sgSik1_2nd-hU6-sgSik3_2nd
Plasmid#177219PurposeExpresses a Sik3-targeting and a non-targeting gRNAs and Cre-recombinaseDepositorInsertsgSik1_2nd/sgSik3_2nd
UseLentiviralTagsExpressionMutationPromotermU6/hU6Available SinceDec. 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLyGo-Ec-1
Plasmid#163127PurposepLyGo cloning vector for cytoplasmic expression in E. coli of a sequence of interest (LPMO). Vector encoding Ubiquitin with an internal His10-tag and the LyGo cassette (SapI-ccdB-SapI)DepositorTypeEmpty backboneUseSynthetic BiologyTagsUbiquitinExpressionBacterialMutationPromoterT7Available SinceMay 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
p663-UBC-miniTurbo-V5-DYRK4_IDG-K
Plasmid#158184PurposeGateway destination clone of DYRK4 (human) tagged with N-terminal miniTurbo-V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomicsDepositorInsertminiTurbo-V5-DYRK4 (DYRK4 Human)
UseLentiviral; Gateway destinationTagsminiTurbo-V5ExpressionMammalianMutationPromoterUbiquitinAvailable SinceOct. 19, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMA4008
Plasmid#70109PurposemtDNA destruction/production of rho-0 cellsDepositorInsertsEGFP
Herpes simplex virus UL12.5M185
UseTagsnoneExpressionMammalianMutationN-terminal deletion of UL12.5PromoterCMV and RSVAvailable SinceMay 3, 2016AvailabilityAcademic Institutions and Nonprofits only -
pHR-UCOE-EF1a-Zim3-dCas9-P2A-GFP
Plasmid#188778PurposedCas9 with an N-term Zim3 KRAB-NLS fusion, a C-term HA-2xNLSDepositorInsertZim3-dCas9
UseLentiviralTagsHA-2xNLS and Zim3 KRAB-NLS fusionExpressionMutationPromoterEF1aAvailable SinceOct. 18, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSLQ10530
Plasmid#239266PurposeExpresses NES-dCas13b-ABI for CRISPR-TO perturbation in the cytoplasm of cell linesDepositorInsertNES-dPspCas13b-EGFP-ABI
UseCRISPR and LentiviralTagsFlag tag and V5 tagExpressionMammalianMutationH133A and H1058A in PspCas13bPromoterEF1aAvailable SinceSept. 10, 2025AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-HA-eLACCO2.1-NGR
Plasmid#208023PurposeBiosensor for extracellular L-lactateDepositorInserteLACCO2.1
UseTagsExpressionMammalianMutationPromoterAvailable SinceOct. 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
pEYFP-N3-EPAC1
Plasmid#113110PurposeHuman EPAC1 gene was fused in-frame and upstream from the enhanced YFP gene in pEYFP-N3 vector (Clonetech).DepositorAvailable SinceJan. 23, 2019AvailabilityAcademic Institutions and Nonprofits only -
P427-FBP_13
Plasmid#139704PurposeT-DNA encoding Fungal Bioluminescent Pathway Components, complete pathwayDepositorInsertpSlH4:NnCPH:tSlH4, pSlRBCS2:AsNpga:tAtug7, pUBQ1:NhH3H:tNos, p35S:NnHisps:t35S, pAtELF4:NnLuz:trbcse9
UseLuciferaseTagsExpressionPlantMutationPromotervariousAvailable SinceMay 29, 2020AvailabilityAcademic Institutions and Nonprofits only -
mCherry-Lifeact-7
Plasmid#54491PurposeLocalization: Action, Excitation: 587, Emission: 610DepositorTypeEmpty backboneUseTagsmCherry-LifeactExpressionMammalianMutationPromoterAvailable SinceJune 20, 2014AvailabilityAcademic Institutions and Nonprofits only -
pMXs-mAscl1
Plasmid#233152PurposeRetroviral expression of Ascl1DepositorAvailable SinceMarch 13, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCR3.1-mGM-CSF
Plasmid#74465Purposeexpression of mGM-CSF in mammalian cellsDepositorInsertgranulocyte-monocyte colony-stimulating factor (Csf2 Mouse)
UseTagsExpressionMammalianMutationPromoterCMVAvailable SinceApril 7, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCM189-ParB1::mCherry
Plasmid#87253PurposeExpresses ParB1-mCherry in yeast. Also known as pOR1-mchDepositorInsertParB1
UseTagsmCherryExpressionYeastMutationPromoterpBAD/tetOAvailable SinceAug. 25, 2017AvailabilityAcademic Institutions and Nonprofits only