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Showing: 4201 - 4230 of 160219 results

  4. UbC-blasticidine-P2A-TETon-3G transactivator

    Plasmid
    #200191
    Purpose
    A TETon activator, which combined with the DiLiCre 2.0 plasmid, drives the expression of DiLiCre 2.0 upon doxycycline. DiLiCre 2.0 is a cre recombinase that is activated by violet light.
    Depositor
    Jacco van Rheenen
    Article
    Vizoso et al Nat Commun. 2022 Oct 28;13(1):6442. doi:
    Insert
    blasticidine - P2A - TETon-3G transactivator
    Use
    Lentiviral
    Tags
    Expression
    Bacterial and Mammalian
    Mutation
    Promoter
    UbC
    Available since
    Aug. 30, 2023
    Availability
    Academic Institutions and Nonprofits only
    200191-plasmid-map-sequence-id-405986

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  7. pGWB-nLUC

    Plasmid
    #174050
    Purpose
    Gateway compatible plasmid containing N-terminal luciferase for split-luciferase complementation assay
    Depositor
    Alberto Macho
    Article
    Yu et al PLoS Pathog. 2020 Sep 25;16(9):e1008933.
    Type
    Empty backbone
    Use
    Luciferase
    Tags
    N-terminal luciferase
    Expression
    Plant
    Mutation
    Promoter
    Available since
    Sept. 22, 2021
    Availability
    Academic Institutions and Nonprofits only
    174050-plasmid-map-sequence-id-342879

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  8. pC-sleeping beauty

    Plasmid
    #65487
    Purpose
    HSP-driven SB100X Sleeping Beauty transposase in pCasper backbone.
    Depositor
    Guillaume Filion
    Insert
    sleeping beauty transposase
    Use
    Tags
    Expression
    Insect
    Mutation
    Promoter
    HSP
    Available since
    Dec. 19, 2017
    Availability
    Academic Institutions and Nonprofits only
    65487-plasmid-map-sequence-id-117817

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  9. pCRISPRa_VPR_yl

    Plasmid
    #107677
    Purpose
    CRISPR-dCas9-VPR vector for Yarrowia lipolytica, expressing dCas9-VPR and AvrII site for sgRNA insertion
    Depositor
    Ian Wheeldon
    Article
    Schwartz et al Biotechnol J. 2018 May 5:e1700584. doi:
    Inserts
    Codon optimized dCas9-VPR
    sgRNA expression cassette
    Use
    CRISPR and Synthetic Biology
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    SCR1'-tRNA and UAS1B8-TEF(136)
    Available since
    May 17, 2018
    Availability
    Academic Institutions and Nonprofits only
    107677-plasmid-map-sequence-id-209648

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  11. pET28a-SnoopCatcher

    Plasmid
    #72322
    Purpose
    Bacterial expression of SnoopCatcher, an engineered protein forming a spontaneous covalent bond with its cognate peptide partner SnoopTag.
    Depositor
    Mark Howarth
    Article
    Veggiani et al Proc Natl Acad Sci U S A. 2016 Jan 19. p
    Insert
    pET28a-SnoopCatcher
    Use
    Tags
    His6 tag
    Expression
    Bacterial
    Mutation
    changed G842T and D848G of S. pneumoniae adhesin …
    Promoter
    T7
    Available since
    Feb. 12, 2016
    Availability
    Academic Institutions and Nonprofits only
    72322-plasmid-map-sequence-id-137253

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  14. pTK Cas9-2A-Citrine

    Plasmid
    #92393
    Purpose
    Enhancer/reporter plasmid for tissue-specific expression of Cas9 with 2A-Citrine reporter. Contains BsmBI-flanked LacZ cloning cassette for rapid GoldenGate-based cloning of specific enhancers.
    Depositor
    Tatjana Sauka-Spengler
    Article
    Williams et al Development. 2018 Feb 23;145(4). pii: de
    Insert
    Cas9 2A Citrine
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    thymidine kinase promoter
    Available since
    Aug. 24, 2017
    Availability
    Academic Institutions and Nonprofits only
    92393-plasmid-map-sequence-id-190866

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  16. pAAV-Ef1a-fDIO-GCaMP6s (AAV8)

    Viral Prep
    #105714-AAV8
    Purpose
    Ready-to-use AAV8 particles produced from pAAV-Ef1a-fDIO-GCaMP6s (#105714). In addition to the viral particles, you will also receive purified pAAV-Ef1a-fDIO-GCaMP6s plasmid DNA. EF1a-driven, Flp recombinase-dependent expression of GCaMP6s calcium sensor. These AAV preparations are suitable purity for injection into animals
    Depositor
    Rylan Larsen
    Promoter
    Ef1a, Human elongation factor-1 alpha
    Tags
    None
    Available since
    Oct. 11, 2019
    Availability
    Academic Institutions and Nonprofits only

  17. pB025

    Plasmid
    #183092
    Purpose
    Expresses FnCas12a in Bacteroides and used for genome editing
    Depositor
    Lei Dai
    Article
    Zheng et al ACS Synth Biol. 2022 Jan 21;11(1):464-47
    Insert
    FnCas12a, gRNA, Promoter, HAB, TetR,
    Use
    CRISPR and Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    P1TDPGH023
    Available since
    Aug. 1, 2022
    Availability
    Academic Institutions and Nonprofits only
    183092-plasmid-map-sequence-id-368147

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  20. pLV-EGFP-Cre

    Plasmid
    #86805
    Purpose
    Lentiviral vector that expresses an EGFP-Cre fusion protein with a nuclear localization signal from the CMV promoter
    Depositor
    Amy Lasek
    Article
    Truitt et al eNeuro. 2016 Oct 31;3(5). pii: ENEURO.02
    Insert
    Cre recombinase
    Use
    Cre/Lox and Lentiviral
    Tags
    EGFP and nuclear localization signal: PKKKRKV
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    March 5, 2018
    Availability
    Academic Institutions and Nonprofits only
    86805-plasmid-map-sequence-id-436570

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  24. pAAV-hSyn-hM3D(Gq)-mCherry (AAV8)

    Viral Prep
    #50474-AAV8
    Purpose
    Ready-to-use AAV8 particles produced from pAAV-hSyn-hM3D(Gq)-mCherry (#50474). In addition to the viral particles, you will also receive purified pAAV-hSyn-hM3D(Gq)-mCherry plasmid DNA. hSyn-driven hM3D(Gq) receptor with an mCherry reporter for CNO-induced neuronal activation. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Bryan Roth
    Promoter
    Syn
    Tags
    mCherry
    Available since
    Oct. 3, 2016
    Availability
    Academic Institutions and Nonprofits only

  25. pAAV-hSyn-EGFP (AAV5)

    Viral Prep
    #50465-AAV5
    Purpose
    Ready-to-use AAV5 particles produced from pAAV-hSyn-EGFP (#50465). In addition to the viral particles, you will also receive purified pAAV-hSyn-EGFP plasmid DNA. hSyn-driven EGFP expression. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Bryan Roth
    Promoter
    Syn
    Tags
    EGFP
    Available since
    Dec. 21, 2016
    Availability
    Academic Institutions and Nonprofits only

  28. pAAV-S5E2-ChR2-mCherry (AAV PHP.eB)

    Viral Prep
    #135634-PHPeB
    Purpose
    Ready-to-use AAV PHP.eB particles produced from pAAV-S5E2-ChR2-mCherry (#135634). In addition to the viral particles, you will also receive purified pAAV-S5E2-ChR2-mCherry plasmid DNA. Expression of ChR2-mCherry under the control of the E2 regulatory element. These AAV were produced with the PHPeB serotype, which permits efficient transduction of the central nervous system. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Jordane Dimidschstein
    Article
    Vormstein-Schneider et al Nat Neurosci. 2020 Dec;23(12):1629-1636.
    Promoter
    Tags
    mCherry
    Available since
    Oct. 28, 2021
    Availability
    Academic Institutions and Nonprofits only

  30. DiLiCre 2.0

    Plasmid
    #198752
    Purpose
    A Cre recombinase that is expressed upon doxycycline when combined with the UbC-blasticidine-P2A-TETon-3G transactivator. DiLiCre 2.0 is a cre recombinase that is activated by violet light.
    Depositor
    Jacco van Rheenen
    Article
    Vizoso et al Nat Commun. 2022 Oct 28;13(1):6442. doi:
    Insert
    Cre-PhoCI-ERT2-ERT2
    Use
    Lentiviral
    Tags
    Cre, kept in a photocleavable site, followed by 2…
    Expression
    Bacterial and Mammalian
    Mutation
    Promoter
    TRE3GV
    Available since
    July 7, 2023
    Availability
    Academic Institutions and Nonprofits only
    198752-plasmid-map-sequence-id-397647

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Showing: 4201 - 4230 of 160219 results