319,490 results

1. EC70198

   Plasmid

   #209459

   Purpose

   Cloning protospacers for Cas9 editing

   Depositor

   Cristobal Uauy, Wendy Harwood

   Article

   Lawrenson et al Research Square, 12 March 2024, PREPRINT

   Insert

   Cas9 sgRNA
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   TaU3

   Available since

   Nov. 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. EC70200

   Plasmid

   #209461

   Purpose

   Cloning protospacers for Cas9 editing

   Depositor

   Cristobal Uauy, Wendy Harwood

   Article

   Lawrenson et al Research Square, 12 March 2024, PREPRINT

   Insert

   Cas9 sgRNA
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   TaU3

   Available since

   Nov. 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. EC70198

   Plasmid

   #209459

   Purpose

   Cloning protospacers for Cas9 editing

   Depositor

   Cristobal Uauy, Wendy Harwood

   Article

   Lawrenson et al Research Square, 12 March 2024, PREPRINT

   Insert

   Cas9 sgRNA
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   TaU3

   Available since

   Nov. 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. EC70200

   Plasmid

   #209461

   Purpose

   Cloning protospacers for Cas9 editing

   Depositor

   Cristobal Uauy, Wendy Harwood

   Article

   Lawrenson et al Research Square, 12 March 2024, PREPRINT

   Insert

   Cas9 sgRNA
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   TaU3

   Available since

   Nov. 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pET-3c-rhVTN-NC

   Plasmid

   #30226

   Depositor

   James Thomson

   Article

   Chen et al Nat Methods. 2011 May;8(5):424-9. doi: 1

   Insert

   rhVitronectin with N and C terminus cleavages (VTN Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   deleted amino acids: 399-478, 20-62

   Promoter

   Available since

   Sept. 26, 2011

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pET-3c-rhVTN-NC

   Plasmid

   #30226

   Depositor

   James Thomson

   Article

   Chen et al Nat Methods. 2011 May;8(5):424-9. doi: 1

   Insert

   rhVitronectin with N and C terminus cleavages (VTN Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   deleted amino acids: 399-478, 20-62

   Promoter

   Available since

   Sept. 26, 2011

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pFUSE-CHIg-hG1_AT118-L-Fc LALA + PG

   Plasmid

   #222852

   Purpose

   Mammalian expression plasmid for secretion of AT118-L a humanized nanobody antagonist of the human Angiotensin II type I receptor (AT1R) fused to human IgG1 Fc

   Depositor

   Andrew Kruse

   Article

   Skiba et al Nat Chem Biol. 2024 May 14. doi: 10.1038

   Insert

   AT118-L
   

   Use

   Tags

   IgG1_Fc

   Expression

   Mammalian

   Mutation

   L234A, L235A, P329G

   Promoter

   Available since

   Feb. 10, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pFUSE-CHIg-hG1_AT118-L-Fc LALA + PG

   Plasmid

   #222852

   Purpose

   Mammalian expression plasmid for secretion of AT118-L a humanized nanobody antagonist of the human Angiotensin II type I receptor (AT1R) fused to human IgG1 Fc

   Depositor

   Andrew Kruse

   Article

   Skiba et al Nat Chem Biol. 2024 May 14. doi: 10.1038

   Insert

   AT118-L
   

   Use

   Tags

   IgG1_Fc

   Expression

   Mammalian

   Mutation

   L234A, L235A, P329G

   Promoter

   Available since

   Feb. 10, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pAAV.GFAP.SV40.eGFP.SV40(polyA)

   Plasmid

   #195558

   Purpose

   Expresses eGFP under control of short GFAP promoter

   Depositor

   Kevin Park

   Article

   Bray et al Neuron. 2019 Aug 21;103(4):642-657.e7. d

   Insert

   eGFP
   

   Use

   AAV

   Tags

   No tags

   Expression

   Mammalian

   Mutation

   Promoter

   short GFAP

   Available since

   Feb. 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pAAV.GFAP.SV40.eGFP.SV40(polyA)

    Plasmid

    #195558

    Purpose

    Expresses eGFP under control of short GFAP promoter

    Depositor

    Kevin Park

    Article

    Bray et al Neuron. 2019 Aug 21;103(4):642-657.e7. d

    Insert

    eGFP
    

    Use

    AAV

    Tags

    No tags

    Expression

    Mammalian

    Mutation

    Promoter

    short GFAP

    Available since

    Feb. 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. TFORF0498

    Plasmid

    #143708

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    EGR3 (EGR3 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Sept. 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. TFORF0498

    Plasmid

    #143708

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    EGR3 (EGR3 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Sept. 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. TFORF0497

    Plasmid

    #143707

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    EGR3 (EGR3 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Sept. 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. TFORF0497

    Plasmid

    #143707

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    EGR3 (EGR3 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Sept. 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pColE1-ISceI

    Plasmid

    #179578

    Purpose

    Expresses I-SceI from an arabinose promoter

    Depositor

    Kevin Solomon

    Article

    Lee et al ACS Synth Biol. 2022 Jan 21;11(1):53-60.

    Insert

    I-SceI (I-SceI Budding Yeast)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 21, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pColE1-ISceI

    Plasmid

    #179578

    Purpose

    Expresses I-SceI from an arabinose promoter

    Depositor

    Kevin Solomon

    Article

    Lee et al ACS Synth Biol. 2022 Jan 21;11(1):53-60.

    Insert

    I-SceI (I-SceI Budding Yeast)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 21, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. PKM2

    Plasmid

    #25538

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Cheryl Arrowsmith

    Insert

    PKM2 (PKM Human)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 24, 2010

    Availability

    Industry, Academic Institutions, and Nonprofits

18. PKM2

    Plasmid

    #25538

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Cheryl Arrowsmith

    Insert

    PKM2 (PKM Human)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 24, 2010

    Availability

    Industry, Academic Institutions, and Nonprofits

19. pcDNA3-HA-MKRN1

    Plasmid

    #78745

    Purpose

    To overexpress MKRN1 in Mammalian Cells

    Depositor

    Jaewhan Song

    Article

    Lee et al EMBO J. 2009 Jul 22;28(14):2100-13. doi:

    Insert

    MKRN1 (MKRN1 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 15, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pcDNA3-HA-MKRN1

    Plasmid

    #78745

    Purpose

    To overexpress MKRN1 in Mammalian Cells

    Depositor

    Jaewhan Song

    Article

    Lee et al EMBO J. 2009 Jul 22;28(14):2100-13. doi:

    Insert

    MKRN1 (MKRN1 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 15, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pMpGWB106

    Plasmid

    #68560

    Purpose

    Gateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plants

    Depositor

    Takayuki Kohchi

    Article

    Ishizaki et al PLoS One. 2015 Sep 25;10(9):e0138876. do

    Type

    Empty backbone

    Use

    Tags

    Citrine

    Expression

    Plant

    Mutation

    Promoter

    Cauliflower mosaic virus 35S promoter

    Available since

    July 13, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pMpGWB106

    Plasmid

    #68560

    Purpose

    Gateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plants

    Depositor

    Takayuki Kohchi

    Article

    Ishizaki et al PLoS One. 2015 Sep 25;10(9):e0138876. do

    Type

    Empty backbone

    Use

    Tags

    Citrine

    Expression

    Plant

    Mutation

    Promoter

    Cauliflower mosaic virus 35S promoter

    Available since

    July 13, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pGGF_RT_FAST_Tq2CFP

    Plasmid

    #136981

    Purpose

    Goldengate/Greengate module marker for transgenic Arabidopsis seed coat fluorescence

    Depositor

    Klaus Theres

    Insert

    seed coat marker, replaced RFP w/ Tq2CFP. Replaced NOS terminator w/ Arabidopsis HSP18-2 terminator
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    BsaI site domestication in pOLE1:OLE1-RFP 688 G &…

    Promoter

    Available since

    Jan. 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pGGF_RT_FAST_Tq2CFP

    Plasmid

    #136981

    Purpose

    Goldengate/Greengate module marker for transgenic Arabidopsis seed coat fluorescence

    Depositor

    Klaus Theres

    Insert

    seed coat marker, replaced RFP w/ Tq2CFP. Replaced NOS terminator w/ Arabidopsis HSP18-2 terminator
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    BsaI site domestication in pOLE1:OLE1-RFP 688 G &…

    Promoter

    Available since

    Jan. 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pGM34DEST

    Plasmid

    #74756

    Purpose

    Encodes for the 5X repeat of the QUAS response element, followed by the delta pes-10 minimal promoter

    Depositor

    Keith Yamamoto, Jordan Ward

    Article

    Monsalve et al Genetics. 2019 Feb;211(2):419-430. doi:

    Insert

    Gateway cassette
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Promoter

    Available since

    Oct. 23, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pGM34DEST

    Plasmid

    #74756

    Purpose

    Encodes for the 5X repeat of the QUAS response element, followed by the delta pes-10 minimal promoter

    Depositor

    Keith Yamamoto, Jordan Ward

    Article

    Monsalve et al Genetics. 2019 Feb;211(2):419-430. doi:

    Insert

    Gateway cassette
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Promoter

    Available since

    Oct. 23, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pEGFP_sfCherry2(11)_zyxin

    Plasmid

    #83034

    Purpose

    Expresses sfCherry2(11) tagged zyxin (human) in mammalian cells

    Depositor

    Bo Huang

    Article

    Feng et al Nat Commun. 2017 Aug 29;8(1):370. doi: 1

    Insert

    sfCherry2(11)_zyxin
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 29, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pEGFP_sfCherry2(11)_zyxin

    Plasmid

    #83034

    Purpose

    Expresses sfCherry2(11) tagged zyxin (human) in mammalian cells

    Depositor

    Bo Huang

    Article

    Feng et al Nat Commun. 2017 Aug 29;8(1):370. doi: 1

    Insert

    sfCherry2(11)_zyxin
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 29, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pFRT/TO/HIS/FLAG/HA-YTHDF2

    Plasmid

    #38089

    Depositor

    Markus Landthaler

    Article

    Baltz et al Mol Cell. 2012 Jun 8;46(5):674-90.

    Insert

    YTHDF2 YTH domain family, member 2 (YTHDF2 Human)
    

    Use

    Tags

    HIS/FLAG/HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 17, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pFRT/TO/HIS/FLAG/HA-YTHDF2

    Plasmid

    #38089

    Depositor

    Markus Landthaler

    Article

    Baltz et al Mol Cell. 2012 Jun 8;46(5):674-90.

    Insert

    YTHDF2 YTH domain family, member 2 (YTHDF2 Human)
    

    Use

    Tags

    HIS/FLAG/HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 17, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge