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pKLV2-EF1a-Cas9Bsd-W

Plasmid

#68343

Purpose

Lentiviral vector expressing Cas9 fused with the Blasticidin resistant gene at the C-terminus

Depositor

Kosuke Yusa

Publication

Tzelepis et al Cell Rep. 2016 Oct 18;17(4):1193-1205. d

Insert

EF1a-Cas9 cassette, WPRE

Use

CRISPR and Lentiviral

Tags

Cas9 fused with Flag-tag and a NLS at the N termi…

Promoter

EF1a

Available since

Sept. 23, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

40 Articles

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pCW57-MCS1-P2A-MCS2 (GFP)

Plasmid

#80924

Purpose

All-in-one doxycycline inducible lentiviral vector for expression of one or two genes using the P2A self-cleaving peptide.

Depositor

Adam Karpf

Publication

Barger et al Cancers (Basel). 2019 Feb 21;11(2). pii:

Type

Empty backbone

Use

Lentiviral; Doxycycline inducible; p2a self cleav…

Expression

Mammalian

Promoter

TRE

Available since

Aug. 9, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pET28c-F30-2xdBroccoli

Plasmid

#66843

Purpose

Expresses two dimeric Broccoli aptamers in the F30 scaffold in bacterial cells

Depositor

Samie Jaffrey

Publication

Filonov et al Curr Protoc Chem Biol. 2016 Mar 16;8(1):

Insert

Two dimeric Broccoli aptamers in the F30 scaffold

Expression

Bacterial

Promoter

T7

Available since

July 13, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles

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pcDNA3/Flag-WTAP

Plasmid

#53741

Purpose

For expression of WTAP (Wilms tumor 1 associated protein) in human cells

Depositor

Chuan He

Publication

Liu et al Nat Chem Biol. 2014 Feb;10(2):93-5. doi:

Insert

Wilms tumor 1 associated protein (WTAP Human)

Tags

FLAG tag

Expression

Mammalian

Promoter

T7

Available since

Oct. 7, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

8 Articles

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Dendra2-Lifeact-7

Plasmid

#54694

Purpose

Localization: Actin, Excitation: 490 / 553, Emission: 507 / 573

Depositor

Michael Davidson

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Lifeact-Dendra2

Expression

Mammalian

Available since

July 25, 2014

Availability

Industry, Academic Institutions, and Nonprofits

Cited by

9 Articles

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DRH296: FCK-Optopatch2

Plasmid

#51694

Purpose

Coexpression plasmid of QuasAr2 and CheRiff for all-optical electrophysiology

Depositor

Adam Cohen

Publication

Hochbaum et al Nat Methods. 2014 Aug;11(8):825-33. doi:

Insert

Optopatch2

Use

Lentiviral

Expression

Mammalian

Promoter

CaMKIIa

Available since

June 23, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

8 Articles

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pNatB (pACYCduet-naa20-naa25)

Plasmid

#53613

Purpose

Allows expression of the fission yeast NatB complex - chloramphenicol marker

Depositor

Dan Mulvihill

Publication

Johnson et al PLoS One. 2010 Dec 23;5(12):e15801. doi:

Inserts

naa20 (naa20 Fission Yeast)
naa25 (naa25 Fission Yeast)

Expression

Bacterial

Promoter

T7

Available since

June 20, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

17 Articles

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pCAG-Autobow

Plasmid

#45182

Depositor

Joshua Sanes

Publication

Cai et al Nat Methods. 2013 May 5;10(6):540-7. doi

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

pCAG-Autobow

Expression

Mammalian

Promoter

CAG

Available since

Nov. 19, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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tetO-ALN

Plasmid

#43918

Depositor

John Gearhart

Publication

Addis et al PLoS One. 2011;6(12):e28719. doi: 10.137

Insert

ASCL1-2A-LMX1B-2A-NURR1 (ASCL1, LMX1B, NR4A2 Human)

Use

Lentiviral; Doxycycline inducible

Tags

6xHis and V5

Expression

Mammalian

Mutation

Gylcine-serine-glycine (GSG) linker followed by v…

Promoter

TRE promoter, Tet-ON

Available since

March 5, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pCAGGS-Flag-hsDicer

Plasmid

#41584

Depositor

Phil Sharp

Publication

Gurtan et al RNA. 2012 Jun;18(6):1116-22. Epub 2012 A

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

hsDicer (DICER1 Human)

Tags

Flag

Expression

Mammalian

Mutation

None

Promoter

CMV

Available since

Jan. 30, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

17 Articles

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LeGO-C2

Plasmid

#27339

Depositor

Boris Fehse

Publication

Weber et al Mol Ther. 2008 Apr . 16(4):698-706.

Insert

SFFV promoter, mCherry

Use

Lentiviral

Expression

Mammalian

Available since

April 24, 2012

Availability

Academic Institutions and Nonprofits only

Cited by

46 Articles

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pcDNA3 IKKe flag

Plasmid

#26201

Depositor

Tom Maniatis

Publication

Fitzgerald et al Nat Immunol. 2003 May;4(5):491-6.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

I-kappa-B kinase epsilon (IKBKE Human)

Tags

Flag

Expression

Mammalian

Promoter

CMV

Available since

Aug. 24, 2011

Availability

Academic Institutions and Nonprofits only

Cited by

13 Articles

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AP2u2-mCherry

Plasmid

#27672

Depositor

Christien Merrifield

Publication

Taylor et al PLoS Biol. 2011 Mar . 9(3):e1000604.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Ap2m1 (Ap2m1 Rat)

Tags

mCherry

Expression

Mammalian

Available since

May 4, 2011

Availability

Academic Institutions and Nonprofits only

Cited by

10 Articles

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Flag-hPIASy

Plasmid

#15208

Depositor

Ke Shuai

Publication

Liu et al Proc Natl Acad Sci U S A. 2001 Mar 13. 9

Insert

PIASy (PIAS4 Human)

Tags

Flag

Expression

Mammalian

Available since

Feb. 8, 2008

Availability

Academic Institutions and Nonprofits only

Cited by

15 Articles

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pEGFP-N1-ACTR3

Plasmid

#8462

Depositor

Matthew Welch

Publication

Welch et al J Cell Biol 1997 Jul 28;138(2):375-84.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

ACTR3 (ACTR3 Human)

Tags

GFP

Expression

Mammalian

Available since

Aug. 7, 2006

Availability

Academic Institutions and Nonprofits only

Cited by

7 Articles

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pcDNA3 HA 14-3-3 sigma

Plasmid

#11946

Depositor

Michael Yaffe

Publication

Wilker et al J Biol Chem. 2005 May 13. 280(19):18891-

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Stratifin (SFN Human)

Tags

HA

Expression

Mammalian

Available since

May 17, 2006

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles

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pcDNA3 HA Arf6 ActQ67L

Plasmid

#10835

Depositor

Thomas Roberts

Publication

Furman et al J Biol Chem. 2002 Mar 8. 277(10):7962-9.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Arf6 Q67L (ARF6 Human)

Tags

HA

Expression

Mammalian

Mutation

Q67L. Defective in GTP hydrolysis. Constitutive…

Available since

April 4, 2006

Availability

Academic Institutions and Nonprofits only

Cited by

6 Articles

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pcDNA3-10His-mStayGold (E138D)

Plasmid

#211363

Purpose

Mammalian expression of the bright and photostable monomeric StayGold fluorescent protein (E138D). Contains a N-terminal 10xHis tag.

Depositor

Mohan Balasubramanian

Publication

Ivorra-Molla et al Nat Biotechnol. 2023 Dec 11. doi: 10.103

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

mStayGold

Tags

10xHis-tag

Expression

Mammalian

Mutation

E138D

Promoter

CMV

Available since

Dec. 20, 2023

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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Lenti-human-TF-gRNA-puromycin

Pooled library

#162275

Purpose

Knockout library targeting a set of human transcription factors for loss-of-function screening.

Depositor

Chunliang Li

Publication

Zhang et al Elife. 2020 Oct 1;9. pii: 57858. doi: 10

Has service

Lentiviral Prep

Species

Homo sapiens

Use

Lentiviral

Available since

Dec. 15, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles
pLVX-EF1alpha-SARS-CoV-2-orf3a-2xStrep-IRES-Puro

Plasmid

#141383

Purpose

Lentiviral expression of SARS-CoV-2 protein; transient expression and generate lentivirus

Depositor

Nevan Krogan

Publication

Gordon et al Nature. 2020 Jul;583(7816):459-468. doi:

Insert

SARS-CoV-2-orf3a (ORF3a SARS-CoV-2)

Use

Lentiviral

Tags

2xStrep

Expression

Mammalian

Mutation

human codon optimized

Promoter

EF1alpha

Available since

April 8, 2020

Availability

Industry, Academic Institutions, and Nonprofits

Cited by

13 Articles

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