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176,133 results

Showing: 2821 - 2840 of 176133 results

  1. L754

    Plasmid
    #1686
    Depositor
    Andrew Fire
    Type
    Empty backbone
    Tags
    IVSA, rf2128
    Expression
    Worm
    Available Since
    March 7, 2005
    Availability
    Academic Institutions and Nonprofits only
    1686-plasmid-map-sequence-id-296

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  3. eSpCas9(1.1)_No_FLAG_ATP1A1_G2_Dual_sgRNA

    Plasmid
    #86612
    Purpose
    Vector for tandem expression of ATP1A1 G2 sgRNA in combination with a user-specified sgRNA from two independent U6 promoters. Cloning of oligos for second sgRNA using BbsI sites. Px333-like plasmid
    Depositor
    Yannick Doyon
    Article
    Agudelo et al Nat Methods. 2017 Apr 17. doi: 10.1038/n
    Insert
    ATP1A1 G2 sgRNA + user-specified sgRNA + FLAGless enhanced specificity Cas9 (1.1)
    Use
    CRISPR; Co-selection via nhej using ouabain
    Expression
    Mammalian
    Mutation
    K848A, K1003A, & R1060A
    Promoter
    CBh
    Available Since
    March 16, 2017
    Availability
    Academic Institutions and Nonprofits only
    86612-plasmid-map-sequence-id-168849

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  5. pHR_SFFV_H2B_miRFP703

    Plasmid
    #231951
    Purpose
    Lentiviral vector for constitutive expression of the far-red fluorescent protein miRFP703 fused with the H2B nuclear localization tag (SFFV promoter)
    Depositor
    Leonardo Morsut
    Insert
    H2B_miRFP703
    Use
    Lentiviral
    Available Since
    April 28, 2025
    Availability
    Academic Institutions and Nonprofits only
    231951-plasmid-map-sequence-id-470260

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  8. TFORF0211

    Plasmid
    #142484
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    FOXP3 (FOXP3 Human)
    Use
    Lentiviral
    Expression
    Mammalian
    Promoter
    EF1a
    Available Since
    April 27, 2023
    Availability
    Academic Institutions and Nonprofits only
    142484-plasmid-map-sequence-id-391610

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  10. lenti-sgRNA blast

    Plasmid
    #104993
    Purpose
    This 3rd generation lentiviral plasmid expresses a S. pyogenes CRISPR chimeric RNA element with customizable sgRNA from a U6 promoter and blasticidin S resistance from an EF-1a promoter.
    Depositor
    Brett Stringer
    Article
    Stringer et al Sci Rep. 2019 Mar 20;9(1):4902. doi: 10.
    Type
    Empty backbone
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Available Since
    Aug. 10, 2018
    Availability
    Academic Institutions and Nonprofits only
    104993-plasmid-map-sequence-id-342099

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  12. CRBN-NanoLuc Vector

    Plasmid
    #238749
    Purpose
    Express CRBN-NanoLuc Fusion Protein in Mammalian Cells under a CMV promoter
    Depositor
    Promega Corporation
    Has Service
    DNA
    Insert
    CRBN (CRBN Human)
    Tags
    NanoLuc (R)
    Expression
    Mammalian
    Mutation
    None
    Promoter
    CMV
    Available Since
    Sept. 14, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    238749-plasmid-map-sequence-id-481948

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  13. pENN.AAV.CB7.CI.mCerulean.WPRE.RBG

    Plasmid
    #105557
    Purpose
    AAV expression of mCerulean from CB7 promoter
    Depositor
    James M. Wilson
    Has Service
    AAV1 and AAV9
    Insert
    mCerulean
    Use
    AAV
    Expression
    Mammalian
    Promoter
    CB7
    Available Since
    May 15, 2018
    Availability
    Academic Institutions and Nonprofits only
    105557-plasmid-map-sequence-id-202393

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  16. pENN.AAV.CMVs.Pl.Cre.rBG (AAV1)

    Viral Prep
    #105537-AAV1
    Purpose
    Ready-to-use AAV1 particles produced from pENN.AAV.CMVs.Pl.Cre.rBG (#105537). In addition to the viral particles, you will also receive purified pENN.AAV.CMVs.Pl.Cre.rBG plasmid DNA. CMV-driven Cre. These AAV preparations are suitable purity for injection into animals.
    Depositor
    James M. Wilson
    Promoter
    CMV
    Available Since
    June 29, 2018
    Availability
    Academic Institutions and Nonprofits only

  17. CRISPRoff-v2.1

    Plasmid
    #167981
    Purpose
    Expresses CRISPRoff-v2.1 (DNMT3A-DNMT3L-XTEN80-dCas9-HA-2xNLS-BFP-KRAB) downstream of the CAG promoter
    Depositor
    Luke Gilbert
    Article
    Nuñez et al Cell. 2021 Apr 7. pii: S0092-8674(21)003
    Insert
    CRISPRoff-v2.1 (DNMT3A-DNMT3L-dCas9-BFP-KRAB)
    Tags
    2xNLS, DNMT3A-DNMT3L, HA, KRAB, and tagBFP
    Expression
    Mammalian
    Promoter
    CAG
    Available Since
    May 13, 2021
    Availability
    Academic Institutions and Nonprofits only
    167981-plasmid-map-sequence-id-330241

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  20. pCAG-NLS(SV40)x2-rAPOBEC1-gs-XTEN-gs-hdenAsCas12a(E174R/S542R/K548R/D908A)-NLS(nucleoplasmin)-gs-UGI-NLS(SV40)(enAsBE1.2)

    Plasmid
    #114082
    Purpose
    CAG promoter expression plasmid for human codon optimized enAsBE1.2, also called RTW1348
    Depositor
    Keith Joung, Benjamin Kleinstiver
    Article
    Kleinstiver et al Nat Biotechnol. 2019 Feb 11. pii: 10.103
    Insert
    DNase-inactive (D908A) enAsCas12a (E174R/S542R/K548R) fused to N-terminal rAPOBEC1 and C-terminal UGI (enAsBE1.2)
    Tags
    2x SV40 NLS and SV40 NLS
    Expression
    Mammalian
    Mutation
    E174R, S542R, K548R and D908A, human codon optimi…
    Available Since
    Dec. 11, 2018
    Availability
    Academic Institutions and Nonprofits only
    114082-plasmid-map-sequence-id-220504

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Showing: 2821 - 2840 of 176133 results