We narrowed to 9,797 results for: otos

1. pAAV-EF1a-DIO-APEX.NES-P2A-EGFP

   Plasmid

   #182824

   Purpose

   Cre-dependent expression of cytosolic APEX

   Depositor

   Yevgenia Kozorovitskiy

   Article

   Dumrongprechachan et al Nat Commun. 2021 Aug 11;12(1):4855. doi:

   Insert

   APEX.NES-P2A-EGFP
   

   Use

   AAV

   Tags

   EGFP reporter (P2A)

   Expression

   Mutation

   Promoter

   EF1a

   Available since

   May 16, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pKanCMV-mRuby3-18aa-actin

   Plasmid

   #74255

   Purpose

   Mammalian expression of N-terminal fusion of mRuby3 to actin

   Depositor

   Michael Lin

   Article

   Bajar et al Sci Rep. 2016 Feb 16;6:20889. doi: 10.10

   Insert

   actin
   

   Use

   Tags

   mRuby3

   Expression

   Mammalian

   Mutation

   Promoter

   CAG and CMV

   Available since

   April 11, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLV-SI-121

   Plasmid

   #131126

   Purpose

   A-to-G base editing reporter

   Depositor

   Nozomu Yachie

   Article

   Sakata et al Nat Biotechnol. 2020 Jun 1. pii: 10.1038

   Insert

   mutant EGFP
   

   Use

   Lentiviral and Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV promoter

   Available since

   Sept. 23, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pUC19_dTAG-KANSL3-targeting

   Plasmid

   #170755

   Purpose

   Donor plasmid for BSD-P2A-2xHA-dTAG knock-in into the N-terminus of the mouse Kansl3 locus

   Depositor

   Kristian Helin

   Article

   Damhofer et al STAR Protoc. 2021 Jun 2;2(2):100575. doi

   Insert

   BSD-P2A-2xHA-dTAG
   

   Use

   Mouse Targeting

   Tags

   BSD-P2A-2xHA-dTAG

   Expression

   Mutation

   Phenylalanine 36 to valine in FKBP12

   Promoter

   Available since

   June 23, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. hHSF4b WT

   Plasmid

   #118356

   Purpose

   This plasmid expresses human HSF4b WT.

   Depositor

   Lea Sistonen

   Article

   Hietakangas et al Proc Natl Acad Sci U S A. 2006 Jan 3;103

   Insert

   hHSF4b (HSF4 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   WT

   Promoter

   Available since

   Nov. 8, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pEGFP-C2-TetR-KRf-TetR

   Plasmid

   #216270

   Purpose

   Construct 3: TetR - Lipid binding domain KRφ - TetR

   Depositor

   Susumu Tomita

   Article

   Park et al Cell Rep. 2023 Aug 29;42(8):112826. doi:

   Insert

   TetR - Lipid binding motif (KRf) - TetR
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 8, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAAV2-DEST(f)

   Plasmid

   #190226

   Purpose

   Gateway destination vector, empty AAV vector backbone

   Depositor

   Hiroyuki Hioki

   Article

   Sohn et al PLoS One. 2017 Jan 6;12(1):e0169611. doi

   Type

   Empty backbone

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 4, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pHR-CMV-TetO2_3C-Twin-Strep

   Plasmid

   #113883

   Purpose

   Mammalian (inducible) protein expression

   Depositor

   A. Radu Aricescu

   Article

   Elegheert et al Nat Protoc. 2018 Nov 19. pii: 10.1038/s4

   Type

   Empty backbone

   Use

   Lentiviral

   Tags

   3C-Twin-Strep

   Expression

   Mutation

   Promoter

   CMV-MIE-TetO2

   Available since

   Aug. 30, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. Brachyury-eGFP

   Plasmid

   #21063

   Depositor

   Mark Mercola

   Article

   Kita-Matsuo et al PLoS ONE. 2009 . 4(4):e5046.

   Insert

   Brachyury promoter
   

   Use

   Lentiviral

   Tags

   eGFP

   Expression

   Mutation

   Promoter

   Available since

   July 1, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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10. EPB104

    Plasmid

    #68369

    Purpose

    contains human U6 promoter

    Depositor

    Eric-Paul Bennett

    Article

    Lonowski et al Nat Protoc. 2017 Mar;12(3):581-603. doi:

    Insert

    U6 promoter (RNU6-1 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    U6

    Available since

    Feb. 23, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. pGP-AAV-CAG-iGABASnFR2n-WPRE

    Plasmid

    #218884

    Purpose

    AAV-mediated expression of improved GABA sensor (negative change in fluorescence)

    Depositor

    GENIE Project

    Article

    Kolb et al bioRxiv 2025.03.25.644953

    Insert

    iGABASnFR2n
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    S99A F104H R168P

    Promoter

    CAG

    Available since

    June 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCAG-Gdf5

    Plasmid

    #163596

    Purpose

    Expresses GDF-5 in mammalian cells

    Depositor

    Takeshi Imai

    Article

    Aihara et al Cell Rep. 2021 Jun 22;35(12):109276. doi

    Insert

    Gdf5 (Gdf5 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pKanCMV-mClover3-18aa-Tubulin

    Plasmid

    #74253

    Purpose

    Mammalian expression of N-terminal fusion of mClover3 to Tubulin

    Depositor

    Michael Lin

    Article

    Bajar et al Sci Rep. 2016 Feb 16;6:20889. doi: 10.10

    Insert

    Tubulin
    

    Use

    Tags

    mClover3

    Expression

    Mammalian

    Mutation

    Promoter

    CAG and CMV

    Available since

    April 11, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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14. pHR-CMV-TetO2_mRuby2

    Plasmid

    #113894

    Purpose

    Mammalian (inducible) expression of cytosolic mRuby2

    Depositor

    A. Radu Aricescu

    Article

    Elegheert et al Nat Protoc. 2018 Nov 19. pii: 10.1038/s4

    Insert

    monomeric Ruby2
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    CMV-MIE-TetO2

    Available since

    Aug. 27, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pcDNA FLAG TORC3

    Plasmid

    #22976

    Depositor

    Marc Montminy

    Article

    Screaton et al Cell. 2004 Oct 1. 119(1):61-74.

    Insert

    TORC3 (CRTC3 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 22, 2010

    Availability

    Academic Institutions and Nonprofits only

16. pJH3626

    Plasmid

    #179630

    Purpose

    Pacr-2(s)::tomm20::miniSOG-SL2::RFP unc-54 3' UTR C.elegans A/B-MN-specific expression of miniSOG-SL2::RFP

    Depositor

    Mei Zhen

    Article

    Gao et al Elife. 2018 Jan 23;7. pii: 29915. doi: 1

    Insert

    tomm20::miniSOG (tomm-20 Nematode)
    

    Use

    Tags

    RFP

    Expression

    Worm

    Mutation

    Promoter

    Pacr-2(s)

    Available since

    March 2, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. His-Keap1-Tev-Halo

    Plasmid

    #87545

    Purpose

    expresses C-terminal Halo-tagged human Keap1 in mammalian cells

    Depositor

    Yimon Aye

    Article

    Parvez et al Nat Protoc. 2016 Dec;11(12):2328-2356. d

    Insert

    Keap1 (KEAP1 Human)
    

    Use

    Tags

    Halo and His-Tag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. CALID

    Plasmid

    #169708

    Purpose

    Cre-activated light-inducible Dre recombinase

    Depositor

    Dali Li

    Article

    Li et al Proc Natl Acad Sci U S A. 2020 Dec 29;11

    Insert

    a double-floxed inverted Dre246-nMag-T2A-pMag-Dre247
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    none

    Promoter

    Available since

    May 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pHR-CMV-TetO2_mVenus

    Plasmid

    #113893

    Purpose

    Mammalian (inducible) expression of cytosolic mVenus

    Depositor

    A. Radu Aricescu

    Article

    Elegheert et al Nat Protoc. 2018 Nov 19. pii: 10.1038/s4

    Insert

    monomeric Venus
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    CMV-MIE-TetO2

    Available since

    Aug. 30, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pAAV-CAG-Arch-GFP

    Plasmid

    #37810

    Depositor

    Edward Boyden

    Article

    Chow et al Nature. 2010 Jan 7. 463(7277):98-102.

    Insert

    Arch
    

    Use

    AAV; Adeno-associated virus

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    N/A

    Promoter

    CAG

    Available since

    Aug. 8, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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21. pcDNA3-AU1-mTOR-L1460P

    Plasmid

    #19995

    Depositor

    Fuyuhiko Tamanoi

    Article

    Urano et al Proc Natl Acad Sci U S A. 2007 Feb 27. 1

    Insert

    mammalian target of rapamycin-L1460P (Mtor Rat)
    

    Use

    Tags

    AU1

    Expression

    Mammalian

    Mutation

    Changed Leucine 1460 to Proline

    Promoter

    Available since

    Jan. 28, 2009

    Availability

    Academic Institutions and Nonprofits only

22. pYFAC-CH3

    Plasmid

    #168979

    Purpose

    AMA1 based expression in filamentous fungi pYFAC-riboB carrying PalcA-T1-PalcS/M-T2-PaldA expression cassette ; unique restriction site: PacI for PalcA, NotI for PalcS, AsiSI for PalcM, AscI for PaldA

    Depositor

    Yit Heng Chooi

    Article

    Hu et al Chem Sci. 2018 Nov 22;10(5):1457-1465. d

    Type

    Empty backbone

    Use

    Synthetic Biology; Heterologous expression in asp…

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pBADmod1-linker2-gamS

    Plasmid

    #45833

    Depositor

    Vincent Noireaux, Richard Murray

    Article

    Sun et al ACS Synth Biol. 2014 Jun 20;3(6):387-97.

    Insert

    gamS
    

    Use

    Synthetic Biology

    Tags

    Histag

    Expression

    Bacterial

    Mutation

    Promoter

    pBAD

    Available since

    May 4, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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24. p3E-V5-mScarlet-I_SV40pA (JDW 968)

    Plasmid

    #224529

    Purpose

    A Gateway compatible 3' entry clone containing an V5 mScarlet fusion followed by SV40 late polyA

    Depositor

    Joshua Wythe

    Article

    Gillespie et al bioRxiv 2024.07.13.603267

    Insert

    V5-mScarlet-I
    

    Use

    Gateway cloning

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. Yellow Cameleon-Nano50/pcDNA3

    Plasmid

    #51964

    Purpose

    mammalian expression of Yellow Cameleon-Nano50, an ultrasensitive Ca2+ indicator with Kd = 50 nM

    Depositor

    Takeharu Nagai

    Article

    Horikawa et al Nat Methods. 2010 Sep;7(9):729-32. doi:

    Insert

    Yellow Cameleon-Nano50
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 18, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. PBMN YFP-ER50

    Plasmid

    #37261

    Depositor

    Thomas Wandless

    Article

    Miyazaki et al J Am Chem Soc. 2012 Mar 7;134(9):3942-5.

    Insert

    ER50 (ESR1 Human)
    

    Use

    Retroviral

    Tags

    HA-YFP

    Expression

    Mutation

    6 mutations, T371A, L384M, M421G, G521R, Y537S, N…

    Promoter

    LTR

    Available since

    Aug. 7, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pAAV-hSyn-Chronos-mT-Sapphire-NRN

    Plasmid

    #149692

    Purpose

    For packaging and expression of Chronos-mT-Sapphire featuring a neuritin (NRN) 3' UTR in neurons via AAV

    Depositor

    Robert Malenka

    Article

    Christoffel et al Proc Natl Acad Sci U S A. 2021 Jun 15;11

    Insert

    Chronos
    

    Use

    AAV

    Tags

    mT-Sapphire

    Expression

    Mammalian

    Mutation

    Promoter

    hSyn

    Available since

    May 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pAAV-TRE-YPet-WPRE

    Plasmid

    #193342

    Purpose

    AAV-mediated expression of YPet under the TRE promoter in mammalian cells

    Depositor

    Takeshi Imai

    Article

    Leiwe et al Nat Commun. 2024 Jun 25;15(1):5279. doi:

    Insert

    YPet
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    TRE

    Available since

    Aug. 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pcDNA3.1(+)-mTagBFP2-MRTF-B

    Plasmid

    #182062

    Purpose

    Mammalian expression vector encoding myocardin-related transcription factor B tagged with mTagBFP2.

    Depositor

    Jin Zhang

    Article

    Zhang et al PLoS Biol. 2020 Sep 3;18(9):e3000866. do

    Insert

    mTagBFP2-MRTF-B (Mrtfb Mouse)
    

    Use

    Tags

    mTagBFP2

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pT7-AsCas12a_crRNA-site4 (MSP3511)

    Plasmid

    #160139

    Purpose

    T7 promoter expression plasmid for in vitro transcription of AsCas12a crRNA with spacer #4

    Depositor

    Keith Joung, Benjamin Kleinstiver

    Article

    Walton et al Nat Protoc. 2021 Feb 5. pii: 10.1038/s41

    Insert

    AsCas12a crRNA with spacer #4 (spacer=GGAATCCCTTCTGCAGCACCTGG)
    

    Use

    In vitro transcription of sgrna from t7 promoter

    Tags

    Expression

    Mutation

    Promoter

    T7

    Available since

    Feb. 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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