We narrowed to 6,990 results for: Cad

1. pMax_BFP_A191G

   Plasmid

   #177823

   Purpose

   Site-specific mutagenesis of tagBFP for the purposes of creating a site-specific U:A mispair for the purposes of measuring UDG activity via Host Cell Reactivation (HCR).

   Depositor

   Leona Samson, Zachary Nagel

   Article

   Nagel et al Proc Natl Acad Sci U S A. 2014 May 6;111

   Insert

   tagBFP_A191G
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   A191G

   Promoter

   CMV

   Available since

   Dec. 21, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA4-ATR-M

   Plasmid

   #53768

   Purpose

   mammalian expression of flag-tagged ATR middle region

   Depositor

   Aziz Sancar

   Article

   Unsal-Kacmaz et al Proc Natl Acad Sci U S A. 2002 May 14;99

   Insert

   ATR (ATR Human)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   middle domain, aa 593-1700*

   Promoter

   CMV

   Available since

   July 8, 2014

   Availability

   Academic Institutions and Nonprofits only

3. pJR019

   Plasmid

   #198834

   Purpose

   Inactivation of G protein subunits through Pertussis toxin (PTX)

   Depositor

   Paul Sternberg

   Article

   Nava et al Proc Natl Acad Sci U S A. 2023 Dec 19;12

   Insert

   15xUAS::PTX-SL2-GFP::let-858 3'UTR
   

   Use

   Tags

   Expression

   Worm

   Mutation

   Promoter

   Available since

   May 2, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCMV3Tag8li-hDDX41-HA

   Plasmid

   #165469

   Purpose

   Human DDX41 mammalian expression vector with C-terminal HA tag.

   Depositor

   Martin Dorf

   Article

   Li et al Proc Natl Acad Sci U S A. 2014 May 13;11

   Insert

   DEAD-box helicase 41 (DDX41 Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   March 17, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pGEX-gp78c/2767

   Plasmid

   #11429

   Depositor

   Allan Weissman

   Article

   Fang et al Proc Natl Acad Sci U S A. 2001 Dec 4. 98

   Insert

   gp78c (AMFR Human)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   n/a

   Promoter

   Available since

   July 19, 2006

   Availability

   Academic Institutions and Nonprofits only

6. pCAG-CFP-SaCas9-HF (without sgRNA)

   Plasmid

   #134470

   Purpose

   Human expression vector for SaCas9-HF variant.

   Depositor

   Zongli Zheng

   Article

   Tan et al Proc Natl Acad Sci U S A. 2019 Sep 30. p

   Insert

   Staphylococcus aureus Cas9 nuclease (SauCas9) with high genome-wide specificity
   

   Use

   CRISPR

   Tags

   SV40NLS-3xFLAG

   Expression

   Mammalian

   Mutation

   R245A, N413A, N419A, R654A

   Promoter

   CAG

   Available since

   Nov. 26, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. Stat1 alpha pRC/CMV

   Plasmid

   #8690

   Depositor

   Jim Darnell

   Article

   Schindler et al Proc Natl Acad Sci U S A 1992 Aug 15;89(

   Insert

   STAT1 alpha (STAT1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 2, 2005

   Availability

   Academic Institutions and Nonprofits only

8. pHeACT-mEGFP-NLS

   Plasmid

   #205003

   Purpose

   The vector contains 1kbp of the upstream and downstream regions of the actin gene from Hypsibius exemplaris, with the mEGFP gene with NLS sequence positioned in between.

   Depositor

   Kazuharu Arakawa

   Article

   Tanaka et al Proc Natl Acad Sci U S A. 2023 Jan 31;12

   Insert

   mEGFP-NLS flanked by 1kbp upstream and downstream of the actin gene from Hypsibius exemplaris
   

   Use

   Tardigrade expression

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Aug. 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. FAM177A1isoform2-GFP

   Plasmid

   #224589

   Purpose

   For expression of FAM177A1-GFP in mammalian cells

   Depositor

   Pietro De Camilli

   Article

   Ugur et al bioRxiv [Preprint]. 2023 Dec 18:2023.12.

   Insert

   FAM177A1 (FAM177A1 Human)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Dec. 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. As0

    Plasmid

    #123158

    Purpose

    Arsenic sensor used in the sensor cell array. pSB4A3 carrying J109-30arsR-t-ParsR-ABS67-30hrpR-30hrpS-t-PhrpLE-30RinA-t-PrinA-30gfp-t

    Depositor

    Baojun Wang

    Article

    Wan et al Nat Chem Biol. 2019 May;15(5):540-548. d

    Insert

    J109-30arsR-t-ParsR-ABS67-30hrpR-30hrpS-t-PhrpLE-30RinA-t-PrinA-30gfp-t
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    J109, ParsR-ABS67, PhrpLE, PrinA

    Available since

    May 9, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pMXs-Ms-c-Myc-W136E

    Plasmid

    #26024

    Depositor

    Shinya Yamanaka

    Article

    Nakagawa et al Proc Natl Acad Sci U S A. 2010 Aug 10;10

    Insert

    c-Myc (Myc Mouse)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    W136E

    Promoter

    Available since

    Aug. 20, 2010

    Availability

    Academic Institutions and Nonprofits only

12. pCMV3FLAG_hGTPBP4

    Plasmid

    #53974

    Purpose

    Mammalian expression plasmid for human GTPBP4 with n-terminal 3xFLAG epitope fused.

    Depositor

    Martin Dorf

    Article

    Li et al Proc Natl Acad Sci U S A. 2014 May 13;11

    Insert

    GTPBP4 (GTPBP4 Human)
    

    Use

    Tags

    3x FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 1, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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13. pET41sIII_GST-ALC1

    Plasmid

    #173932

    Purpose

    Bacterial expression of ALC1

    Depositor

    Joan Conaway

    Article

    Ooi et al Proc Natl Acad Sci U S A. 2021 Sep 7;118

    Insert

    ALC1 (CHD1L Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. AWP-031

    Plasmid

    #213967

    Purpose

    Expresses dPb2Cas12a under an IPTG-inducible promoter and contains a cassette for constitutive CRISPR array expression.

    Depositor

    Alexander Westermann

    Article

    Prezza et al Proc Natl Acad Sci U S A. 2024 Feb 6;121

    Insert

    Cas12a (cas12a Synthetic, Segatella bryantii)
    

    Use

    Tags

    3XFLAG

    Expression

    Bacterial

    Mutation

    D875A to inactivate target nuclease activity

    Promoter

    PcfxA, IPTG inducible

    Available since

    April 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pENTR223-BRLF1(Rta)

    Plasmid

    #195824

    Purpose

    Gateway Entry Clone of Epstein-Barr virus BRLF1

    Depositor

    Eric Johannsen

    Article

    Calderwood et al Proc Natl Acad Sci U S A. 2007 May 1;104

    Insert

    BRLF1 (BRLF1 Epstein-Barr virus)
    

    Use

    Gateway entry vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 19, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pLenti-SPA2G231V

    Plasmid

    #42079

    Depositor

    Christine Garcia

    Article

    Maitra et al Proc Natl Acad Sci U S A. 2012 Dec 18;10

    Insert

    Surfactant Protein -A2 (SFTPA2 Human)
    

    Use

    Lentiviral; Plenti

    Tags

    V5

    Expression

    Bacterial and Mammalian

    Mutation

    Changed Glycine 231 to Valine

    Promoter

    CMV

    Available since

    March 19, 2013

    Availability

    Academic Institutions and Nonprofits only

17. pGEX-KG-Itch/2793

    Plasmid

    #11434

    Depositor

    Allan Weissman

    Article

    Fang et al Proc Natl Acad Sci U S A. 2001 Dec 4. 98

    Insert

    Itch (Itch Mouse)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    n/a

    Promoter

    Available since

    April 5, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pCS2 Notch1 ΔEMV-6MT

    Plasmid

    #41737

    Depositor

    Jeffrey Nye, Raphael Kopan

    Article

    Kopan et al Proc Natl Acad Sci U S A. 1996 Feb 20;93

    Insert

    Notch-1 Extracellular-domain-deleted construct (Notch1 Mouse)
    

    Use

    Tags

    6xMyc

    Expression

    Mammalian

    Mutation

    Contains murine Notch-1 protein fragment lacking …

    Promoter

    Available since

    Jan. 28, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pSCALPs-HA-NFAT1 (4-460)-GFP

    Plasmid

    #88879

    Purpose

    Lentiviral vector expressing the fusion protein HA-NFAT1 (4-460)-GFP

    Depositor

    Silvia Monticelli

    Article

    Leoni et al Proc Natl Acad Sci U S A. 2017 Feb 6. pi

    Insert

    HA-NFAT1 (4-460)-GFP fusion protein (Nfatc2 Mouse)
    

    Use

    Lentiviral

    Tags

    GFP fusion protein and HA tag

    Expression

    Mammalian

    Mutation

    amino acids 4-460 only; L78P & L287P *see bel…

    Promoter

    SFFV (spleen focus forming virus)

    Available since

    May 3, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. Tet-O-FUW-FoxG1

    Plasmid

    #35547

    Depositor

    Marius Wernig

    Article

    Lujan et al Proc Natl Acad Sci U S A. 2012 Feb 14;10

    Insert

    FoxG1 (Foxg1 Mouse)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 27, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. His-MBP-NRBF2

    Plasmid

    #99330

    Purpose

    Mammalian expression of NRBF2, which associates with the PI3KC3-C1 complex

    Depositor

    James Hurley

    Article

    Young et al Proc Natl Acad Sci U S A. 2016 Jul 19;11

    Insert

    Nuclear receptor-binding factor 2 (NRBF2 Human)
    

    Use

    Tags

    His-MBP

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Sept. 8, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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22. Cav2.2 HA pcDNA3

    Plasmid

    #206051

    Purpose

    expression of rabbit Cav2.2 calcium channel with an exofacial double HA tag in domain II

    Depositor

    Annette Dolphin

    Article

    Cassidy et al Proc Natl Acad Sci U S A. 2014 Jun 17;11

    Insert

    cacna1b (CACNA1B Rabbit)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. 896 pcDNA3 T7 Akt1

    Plasmid

    #9003

    Depositor

    William Sellers

    Article

    Ramaswamy et al Proc Natl Acad Sci U S A. 1999 Mar 2. 96

    Insert

    Akt1 (AKT1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 5, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. p-mCherry-C1-mJP-4

    Plasmid

    #79599

    Purpose

    Transient expression

    Depositor

    Yousang Gwack

    Article

    Woo et al Proc Natl Acad Sci U S A. 2016 Mar 8;113

    Insert

    Junctophilin-4 (Jph4 Mouse)
    

    Use

    Tags

    2x mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 29, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pcDNA4-Beclin1 243-450

    Plasmid

    #24392

    Depositor

    Qing Zhong

    Article

    Sun et al Proc Natl Acad Sci U S A. 2008 Dec 9. 10

    Insert

    Beclin1 (BECN1 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Contains amino acids 243-450.

    Promoter

    Available since

    April 1, 2010

    Availability

    Academic Institutions and Nonprofits only

26. pcDNA4-Beclin1 151-241

    Plasmid

    #24390

    Depositor

    Qing Zhong

    Article

    Sun et al Proc Natl Acad Sci U S A. 2008 Dec 9. 10

    Insert

    Beclin1 (BECN1 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Contains amino acids 151-241.

    Promoter

    Available since

    April 1, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. POLArISact

    Plasmid

    #164971

    Purpose

    Expresses POLArISact, an orientation probe that specifically binds to F-actin, in mammalian cells

    Depositor

    Sumio Terada

    Article

    Sugizaki et al Proc Natl Acad Sci U S A. 2021 Mar 16;11

    Insert

    POLArISact
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pRvUBC-mEGFP-NLS

    Plasmid

    #205010

    Purpose

    The vector contains 1kbp of the upstream and downstream regions of the ubiquitin c gene from Ramazzottius varieornatus, with the mEGFP gene with NLS sequence positioned in between.

    Depositor

    Kazuharu Arakawa

    Article

    Tanaka et al Proc Natl Acad Sci U S A. 2023 Jan 31;12

    Insert

    mEGFP-NLS flanked by 1kbp upstream and downstream of the ubiquitin c gene from R. varieornatus
    

    Use

    Tardigrade expression

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. BtuF

    Plasmid

    #92209

    Purpose

    Expression of BtuF which is the vitamin B12 binding protein for BtuCD ABC Transporter

    Depositor

    Douglas Rees

    Article

    Borths et al Proc Natl Acad Sci U S A. 2002 Dec 24;99

    Insert

    BtuF (btuF )
    

    Use

    Tags

    6x His Tag and PelB Signal Sequence

    Expression

    Bacterial

    Mutation

    BtuF signal sequence removed and uses the PelB si…

    Promoter

    T7 Promoter

    Available since

    July 21, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. EGFP-INPP5E

    Plasmid

    #223564

    Purpose

    Expresses the full-length mouse INPP5E (Pharbin)

    Depositor

    Pietro De Camilli

    Article

    Mohd Rafiq et al Proc Natl Acad Sci U S A. 2024 Apr 23;12

    Insert

    INPP5E (Inpp5e Mouse)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge