We narrowed to 22,032 results for: ESC

1. pMRE-Tn5-163

   Plasmid

   #118542

   Purpose

   miniTn5 plasmid to deliver constitutively expressed fluorescent protein genes in bacteria

   Depositor

   Mitja Remus-Emsermann

   Article

   Schlechter et al Front Microbiol. 2018 Dec 12;9:3052. doi

   Insert

   sYFP2
   

   Use

   Minitn5 delivery vector

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Sept. 30, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pT2-7xTcf-NLS-ONL

   Plasmid

   #65713

   Purpose

   Tol2-based Wnt signal reporter plasmid (expresses NLS-ONL)

   Depositor

   Yasushi Okada

   Article

   Takai et al Proc Natl Acad Sci U S A. 2015 Apr 7;112

   Insert

   7xTcf, minCMV, 3xNLS, ONL
   

   Use

   Luciferase

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Jan. 26, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. pRSET a sfGCaMP6s-T78H

   Plasmid

   #100023

   Purpose

   Bacterial expression of fluorescent reporter for calcium signaling, based off of GCaMP6s. cpEGFP replaced with a superfolder cpGFP variant containing T78H mutation

   Depositor

   Wolf Frommer

   Article

   Ast et al Nat Commun. 2017 Sep 5;8(1):431. doi: 10

   Insert

   sfGCaMP6s-T78H
   

   Use

   Tags

   6x HIS tag and Xpress_EK tag

   Expression

   Bacterial

   Mutation

   cpEGFP replaced with superfolder cpGFP and T78H m…

   Promoter

   T7

   Available since

   Oct. 3, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. pKeratin-miRFP703

   Plasmid

   #79990

   Purpose

   Keratin labeling with near-infrared fluorescent protein miRFP703

   Depositor

   Vladislav Verkhusha

   Article

   Shcherbakova et al Nat Commun. 2016 Aug 19;7:12405. doi: 10

   Insert

   Keratin (KRT18 Human)
   

   Use

   Tags

   miRFP703

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   July 20, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. pRSETa-mEos4b-V69T

   Plasmid

   #98572

   Purpose

   photoconvertible fluorescent protein mEos4b-V69T in pRSetA plasmid, photoconvertible by primed conversion mechanism, bacterial overexpression

   Depositor

   Ulrike Endesfelder

   Article

   Turkowyd et al Angew Chem Int Ed Engl. 2017 Jun 2. doi:

   Insert

   mEos4b-V69T
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   March 9, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. shCTL-mlpx-puro

   Plasmid

   #65232

   Purpose

   encodes a non-targeting control shRNA

   Depositor

   Gerardo Ferbeyre

   Article

   Deschenes-Simard et al Genes Dev. 2013 Apr 15;27(8):900-15. doi

   Insert

   non-coding shRNA
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   PGK

   Available since

   Aug. 12, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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7. pRSETb-pcDronpa2-A69T

   Plasmid

   #98578

   Purpose

   photoconvertible fluorescent protein pcDronpa2-A69T in pRSetB plasmid, photoconvertible by primed conversion mechanism, bacterial overexpression

   Depositor

   Ulrike Endesfelder

   Article

   Turkowyd et al Angew Chem Int Ed Engl. 2017 Jun 2. doi:

   Insert

   pcDronpa2-A69T
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   March 9, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCMV-MfBulKRS (human-opti)

   Plasmid

   #164196

   Purpose

   To express a Methanosarcina flavescens derived BulKRS in mammalian cells for genetic code expansion

   Depositor

   Tao Liu

   Article

   Hu et al ACS Synth Biol. 2020 Oct 16;9(10):2723-2

   Insert

   Mf BulKRS(human-opti)
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Y269A;Y347F

   Promoter

   CMV

   Available since

   Feb. 26, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pRSETa-mKikGR-V69T

   Plasmid

   #98574

   Purpose

   photoconvertible fluorescent protein mKikGR-V69T in pRSetA plasmid, photoconvertible by primed conversion mechanism, bacterial overexpression, sequence is codon optimised for E. coli

   Depositor

   Ulrike Endesfelder

   Article

   Turkowyd et al Angew Chem Int Ed Engl. 2017 Jun 2. doi:

   Insert

   mKikGR-V69T
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   March 9, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAMV:nAChRgamma-eGFP

    Plasmid

    #29693

    Depositor

    Henry Lester

    Article

    Pantoja et al Biophys J. 2009 Jan . 96(1):226-37.

    Insert

    GFP tagged Mus musculus cholinergic receptor, nicotinic, gamma (Chrng Mouse)
    

    Use

    Tags

    AMV promotor and poly(A) tail

    Expression

    Bacterial

    Mutation

    eGFP inserted after position S380

    Promoter

    Available since

    July 16, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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11. pMRE-Tn5-164

    Plasmid

    #118544

    Purpose

    miniTn5 plasmid to deliver constitutively expressed fluorescent protein genes in bacteria

    Depositor

    Mitja Remus-Emsermann

    Article

    Schlechter et al Front Microbiol. 2018 Dec 12;9:3052. doi

    Insert

    mOrange2
    

    Use

    Minitn5 delivery vector

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Nov. 26, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pMRE-Tn5-166

    Plasmid

    #118546

    Purpose

    miniTn5 plasmid to deliver constitutively expressed fluorescent protein genes in bacteria

    Depositor

    Mitja Remus-Emsermann

    Article

    Schlechter et al Front Microbiol. 2018 Dec 12;9:3052. doi

    Insert

    mCardinal
    

    Use

    Minitn5 delivery vector

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Nov. 26, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pcDNA3.1(+)-FluoSTEP-EKAR

    Plasmid

    #181858

    Purpose

    Genetically encoded FRET-based sensor for monitoring ERK activity near a protein of interest. Must pair with GFP11-tagged POI to reconstitute donor fluorescence.

    Depositor

    Jin Zhang

    Article

    Tenner et al Sci Adv. 2021 May 21;7(21). pii: 7/21/ea

    Insert

    FluoSTEP-EKAR
    

    Use

    Tags

    mRuby2 and sfGFP(1-10)

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pLenti.hUbC.H2B-CeruleanFP-2A-Dendra2FP.W

    Plasmid

    #126522

    Purpose

    Lentivector that expresses H2B-Cerulean and Dendra2 fluorescent proteins from an internal hUbC promoter.

    Depositor

    Rusty Lansford

    Article

    Huss et al Front Cell Dev Biol. 2019 Mar 28;7:35. d

    Insert

    H2B-CeruleanFP-2A-Dendra2FP
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    hUbC

    Available since

    May 22, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. Nuc-Orange Nano-lantern(Ca2+)/pcDNA3

    Plasmid

    #71349

    Purpose

    Express Nuc-Orange Nano-lantern-based luminescent Ca2+ indicator in mammalian cel

    Depositor

    Takeharu Nagai

    Article

    Takai et al Proc Natl Acad Sci U S A. 2015 Apr 7;112

    Insert

    Orange Nano-lantern(Ca2+)_H2B
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 8, 2015

    Availability

    Academic Institutions and Nonprofits only

16. pRSET a sfMatryoshCaMP6s

    Plasmid

    #100020

    Purpose

    Bacterial expression of fluorescent reporter for calcium signaling, based off of GCaMP6s. Contains a stable reference Large Stokes Shift (LSS) mOrange nested within the reporting superfolder cpGFP.

    Depositor

    Wolf Frommer

    Article

    Ast et al Nat Commun. 2017 Sep 5;8(1):431. doi: 10

    Insert

    sfMatryoshCaMP6s
    

    Use

    Tags

    6x HIS tag and Xpress_EK tag

    Expression

    Bacterial

    Mutation

    cpEGFP replaced with superfolder cpGFP

    Promoter

    T7

    Available since

    Oct. 3, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. pVRa15_524

    Plasmid

    #49668

    Depositor

    Christopher Voigt

    Article

    Rhodius et al Mol Syst Biol. 2013 Oct 29;9:702. doi: 1

    Insert

    ECF15_524
    

    Use

    Synthetic Biology

    Tags

    His6-PreScission

    Expression

    Bacterial

    Mutation

    Codon optimized for E.coli

    Promoter

    pT7-lacO

    Available since

    Jan. 3, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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18. Mito-Cyan Nano-lantern(Ca2+)/pcDNA3

    Plasmid

    #71345

    Purpose

    Express Mito-Cyan Nano-lantern-based luminescent Ca2+ indicator in mammalian cel

    Depositor

    Takeharu Nagai

    Article

    Takai et al Proc Natl Acad Sci U S A. 2015 Apr 7;112

    Insert

    hOTC_Cyan Nano-lantern(Ca2+)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 8, 2015

    Availability

    Academic Institutions and Nonprofits only

19. pMiNGo11-3

    Plasmid

    #126058

    Purpose

    A self-assembling split-GFP based Golden Gate vector to determine protein targeting to mitochondria in plant cell

    Depositor

    Ralf-Bernd Klösgen

    Article

    Sharma et al J Cell Sci. 2019 Jun 3;132(11). pii: jcs

    Inserts

    mitochondria Rieske iron sulphur protein (1-100 aa)
    ccdB cassette (BsaI)
    GFP11x3 (5 aa linker)
    

    Use

    Binary

    Tags

    GFP1-10

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Aug. 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pMiNGo11-7

    Plasmid

    #126057

    Purpose

    A self-assembling split-GFP based Golden Gate vector to determine protein targeting to mitochondria in plant cell

    Depositor

    Ralf-Bernd Klösgen

    Article

    Sharma et al J Cell Sci. 2019 Jun 3;132(11). pii: jcs

    Inserts

    mitochondria Rieske iron sulphur protein (1-100 aa)
    ccdB cassette (BsaI)
    GFP11x7 (5 aa linker)
    

    Use

    Binary

    Tags

    GFP1-10

    Expression

    Plant

    Mutation

    Promoter

    Available since

    June 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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