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We narrowed to 1,236 results for: poli

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Showing: 561 - 600 of 1236 results

  1. pAV-U6+27-Linear-2_PolyU(1)_HairpinDistance(10)nts_Tornado-Corn

    Plasmid
    #159501
    Purpose
    Tests for the impacts 1 uracil in a poly-uracil tract in conjunctiuon with a hairpin 10 nts upstream have on human polymerase III transcription elongation from a U6 promoter.
    Depositor
    Julius Lucks
    Article
    Verosloff et al J Mol Biol. 2021 Jun 25;433(13):166978.
    Insert
    Termination Module:Linear-2_PolyU(1)_HairpinDistance(10)nts
    Expression
    Mammalian
    Promoter
    U6-27
    Available Since
    Oct. 13, 2020
    Availability
    Academic Institutions and Nonprofits only
    159501-plasmid-map-sequence-id-311777

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  2. pAV-U6+27-Linear-2_PolyU(4)_HairpinDistance(10)nts_Tornado-Corn

    Plasmid
    #159502
    Purpose
    Tests for the impacts 4 uracils in a poly-uracil tract in conjunctiuon with a hairpin 10 nts upstream have on human polymerase III transcription elongation from a U6 promoter.
    Depositor
    Julius Lucks
    Article
    Verosloff et al J Mol Biol. 2021 Jun 25;433(13):166978.
    Insert
    Termination Module:Linear-2_PolyU(4)_HairpinDistance(10)nts
    Expression
    Mammalian
    Promoter
    U6-27
    Available Since
    Oct. 13, 2020
    Availability
    Academic Institutions and Nonprofits only
    159502-plasmid-map-sequence-id-311727

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  3. pAV-U6+27-Linear-3_PolyU(1)_HairpinDistance(10)nts_Tornado-Corn

    Plasmid
    #159504
    Purpose
    Tests for the impacts 1 uracil in a poly-uracil tract in conjunctiuon with a hairpin 10 nts upstream have on human polymerase III transcription elongation from a U6 promoter.
    Depositor
    Julius Lucks
    Article
    Verosloff et al J Mol Biol. 2021 Jun 25;433(13):166978.
    Insert
    Termination Module:Linear-3_PolyU(1)_HairpinDistance(10)nts
    Expression
    Mammalian
    Promoter
    U6-27
    Available Since
    Oct. 13, 2020
    Availability
    Academic Institutions and Nonprofits only
    159504-plasmid-map-sequence-id-311728

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  4. pAV-U6+27-Linear-3_PolyU(4)_HairpinDistance(10)nts_Tornado-Corn

    Plasmid
    #159506
    Purpose
    Tests for the impacts 4 uracils in a poly-uracil tract in conjunctiuon with a hairpin 10 nts upstream have on human polymerase III transcription elongation from a U6 promoter.
    Depositor
    Julius Lucks
    Article
    Verosloff et al J Mol Biol. 2021 Jun 25;433(13):166978.
    Insert
    Termination Module:Linear-3_PolyU(4)_HairpinDistance(10)nts
    Expression
    Mammalian
    Promoter
    U6-27
    Available Since
    Oct. 13, 2020
    Availability
    Academic Institutions and Nonprofits only
    159506-plasmid-map-sequence-id-311732

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  5. pAV-U6+27-Linear-3_PolyU(4)_HairpinDistance(0)nts_Tornado-Corn

    Plasmid
    #159508
    Purpose
    Tests for the impacts 4 uracils in a poly-uracil tract in conjunctiuon with an immediately upstream hairpin have on human polymerase III transcription elongation from a U6 promoter.
    Depositor
    Julius Lucks
    Article
    Verosloff et al J Mol Biol. 2021 Jun 25;433(13):166978.
    Insert
    Termination Module:Linear-3_PolyU(4)_HairpinDistance(0)nts
    Expression
    Mammalian
    Promoter
    U6-27
    Available Since
    Oct. 13, 2020
    Availability
    Academic Institutions and Nonprofits only
    159508-plasmid-map-sequence-id-311756

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  6. pAV-U6+27-Linear-3_PolyU(4)_HairpinDistance(16)nts_Tornado-Corn

    Plasmid
    #159511
    Purpose
    Tests for the impacts 4 uracils in a poly-uracil tract in conjunctiuon with a hairpin 16 nts upstream have on human polymerase III transcription elongation from a U6 promoter.
    Depositor
    Julius Lucks
    Article
    Verosloff et al J Mol Biol. 2021 Jun 25;433(13):166978.
    Insert
    Termination Module:Linear-3_PolyU(4)_HairpinDistance(16)nts
    Expression
    Mammalian
    Promoter
    U6-27
    Available Since
    Oct. 13, 2020
    Availability
    Academic Institutions and Nonprofits only
    159511-plasmid-map-sequence-id-311774

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  7. pAV-U6+27-Linear-3_PolyU(4)_HairpinDistance(19)nts_Tornado-Corn

    Plasmid
    #159514
    Purpose
    Tests for the impacts 4 uracils in a poly-uracil tract in conjunctiuon with a hairpin 19 nts upstream have on human polymerase III transcription elongation from a U6 promoter.
    Depositor
    Julius Lucks
    Article
    Verosloff et al J Mol Biol. 2021 Jun 25;433(13):166978.
    Insert
    Termination Module:Linear-3_PolyU(4)_HairpinDistance(19)nts
    Expression
    Mammalian
    Promoter
    U6-27
    Available Since
    Oct. 13, 2020
    Availability
    Academic Institutions and Nonprofits only
    159514-plasmid-map-sequence-id-311759

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  8. pAV-U6+27-Linear-3_PolyU(4)_HairpinDistance(20)nts_Tornado-Corn

    Plasmid
    #159515
    Purpose
    Tests for the impacts 4 uracils in a poly-uracil tract in conjunctiuon with a hairpin 20 nts upstream have on human polymerase III transcription elongation from a U6 promoter.
    Depositor
    Julius Lucks
    Article
    Verosloff et al J Mol Biol. 2021 Jun 25;433(13):166978.
    Insert
    Termination Module:Linear-3_PolyU(4)_HairpinDistance(20)nts
    Expression
    Mammalian
    Promoter
    U6-27
    Available Since
    Oct. 13, 2020
    Availability
    Academic Institutions and Nonprofits only
    159515-plasmid-map-sequence-id-311758

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  9. pAV-U6+27-Linear-2_PolyU(1)_HairpinDistance(0)nts_Tornado-Corn

    Plasmid
    #159499
    Purpose
    Tests for the impacts 1 uracil in a poly-uracil tract in conjunctiuon with an immediately upstream hairpin have on human polymerase III transcription elongation from a U6 promoter.
    Depositor
    Julius Lucks
    Article
    Verosloff et al J Mol Biol. 2021 Jun 25;433(13):166978.
    Insert
    Termination Module:Linear-2_PolyU(1)_HairpinDistance(0)nts
    Expression
    Mammalian
    Promoter
    U6-27
    Available Since
    Oct. 13, 2020
    Availability
    Academic Institutions and Nonprofits only
    159499-plasmid-map-sequence-id-311745

    Enlarge

  10. pAV-U6+27-Linear-1_PolyU(4)_HairpinDistance(10)nts_Tornado-Corn

    Plasmid
    #159498
    Purpose
    Tests for the impacts 4 uracils in a poly-uracil tract in conjunctiuon with a hairpin 10 nts upstream have on human polymerase III transcription elongation from a U6 promoter.
    Depositor
    Julius Lucks
    Article
    Verosloff et al J Mol Biol. 2021 Jun 25;433(13):166978.
    Insert
    Termination Module:Linear-1_PolyU(4)_HairpinDistance(10)nts
    Expression
    Mammalian
    Promoter
    U6-27
    Available Since
    Oct. 13, 2020
    Availability
    Academic Institutions and Nonprofits only
    159498-plasmid-map-sequence-id-311780

    Enlarge

  11. pAV-U6+27-Linear-1_PolyU(4) _HairpinDistance(0)_Tornado-Corn

    Plasmid
    #159496
    Purpose
    Tests for the impacts 4 uracils in a poly-uracil tract in conjunctiuon with an immediately upstream hairpin have on human polymerase III transcription elongation from a U6 promoter.
    Depositor
    Julius Lucks
    Article
    Verosloff et al J Mol Biol. 2021 Jun 25;433(13):166978.
    Insert
    Termination Module:Linear-1_PolyU(4) _HairpinDistance(0)
    Expression
    Mammalian
    Promoter
    U6-27
    Available Since
    Oct. 13, 2020
    Availability
    Academic Institutions and Nonprofits only
    159496-plasmid-map-sequence-id-311762

    Enlarge

  12. pAV-U6+27-Linear-1_PolyU(1)_HairpinDistance(0)nts_Tornado-Corn

    Plasmid
    #159495
    Purpose
    Tests for the impacts 1 uracil in a poly-uracil tract in conjunctiuon with an immediately upstream hairpin have on human polymerase III transcription elongation from a U6 promoter.
    Depositor
    Julius Lucks
    Article
    Verosloff et al J Mol Biol. 2021 Jun 25;433(13):166978.
    Insert
    Termination Module:Linear-1_PolyU(1)_HairpinDistance(0)nts
    Expression
    Mammalian
    Promoter
    U6-27
    Available Since
    Oct. 13, 2020
    Availability
    Academic Institutions and Nonprofits only
    159495-plasmid-map-sequence-id-311767

    Enlarge

  14. pCAG-hPPP3CB-FLAG

    Plasmid
    #179135
    Purpose
    Expression vector of human protein phosphatase 3, catalytic subunit, beta isoform (PPP3CB) tagged with FLAG at C-terminus, CAG promoter, rabbit globin poly(A) signal.
    Depositor
    Masahito Ikawa
    Article
    Miyata et al Proc Natl Acad Sci U S A. 2021 Aug 31;11
    Insert
    protein phosphatase 3, catalytic subunit, beta isoform (PPP3CB Human)
    Tags
    FLAG
    Expression
    Mammalian
    Available Since
    Jan. 25, 2022
    Availability
    Academic Institutions and Nonprofits only
    179135-plasmid-map-sequence-id-352807

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  16. pAAV-SCP1-dSa VPR mini.-2X snRP-1 Hbb

    Plasmid
    #99692
    Purpose
    Expresses dSa Cas9 fused to optimized combination of truncated activation domains from SCP1 promoter with dual snRP1 poly adenylation signal, and Sa sgRNA for Hbb, vector allows for strong activation of mouse Hbb, can be packaged and delivered as AAV
    Depositor
    George Church
    Article
    Vora et al bioRxiv 2018. 298620
    Insert
    dCas9 and gRNA targeting Hbb (Hbb Synthetic)
    Use
    AAV
    Tags
    VPR mini
    Mutation
    dead Cas9
    Available Since
    Jan. 16, 2019
    Availability
    Academic Institutions and Nonprofits only
    99692-plasmid-map-sequence-id-194594

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  18. pCAG-hPPP3CC-FLAG

    Plasmid
    #179162
    Purpose
    Expression vector of human protein phosphatase 3, catalytic subunit, gamma isoform (PPP3CC) tagged with FLAG at C-terminus, CAG promoter, rabbit globin poly(A) signal.
    Depositor
    Masahito Ikawa
    Article
    Miyata et al Science. 2015 Oct 23;350(6259):442-5. do
    Insert
    protein phosphatase 3, catalytic subunit, gamma isoform (PPP3CC Human)
    Tags
    FLAG
    Expression
    Mammalian
    Available Since
    Jan. 25, 2022
    Availability
    Academic Institutions and Nonprofits only
    179162-plasmid-map-sequence-id-352821

    Enlarge

  23. pCAG-mSpata33 (I81A)-PA

    Plasmid
    #179131
    Purpose
    Expression vector of mouse spermatogenesis associated 33 (Spata33) tagged with PA at C-terminus, CAG promoter, rabbit globin poly(A) signal. I81A mutation is introduced in Spata33.
    Depositor
    Masahito Ikawa
    Article
    Miyata et al Proc Natl Acad Sci U S A. 2021 Aug 31;11
    Insert
    spermatogenesis associated 33 (Spata33 Mouse)
    Tags
    PA
    Expression
    Mammalian
    Mutation
    Changed Isoleucine 81 to Alanine
    Available Since
    Jan. 25, 2022
    Availability
    Academic Institutions and Nonprofits only
    179131-plasmid-map-sequence-id-352818

    Enlarge

  24. pCAG-mSpata33 (P79A)-PA

    Plasmid
    #179130
    Purpose
    Expression vector of mouse spermatogenesis associated 33 (Spata33) tagged with PA at C-terminus, CAG promoter, rabbit globin poly(A) signal. P79A mutation is introduced in Spata33.
    Depositor
    Masahito Ikawa
    Article
    Miyata et al Proc Natl Acad Sci U S A. 2021 Aug 31;11
    Insert
    spermatogenesis associated 33 (Spata33 Mouse)
    Tags
    PA
    Expression
    Mammalian
    Mutation
    Changed Proline 79 to Alanine
    Available Since
    Jan. 25, 2022
    Availability
    Academic Institutions and Nonprofits only
    179130-plasmid-map-sequence-id-352804

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  25. pCAG-mSpata33 (I83A)-PA

    Plasmid
    #179132
    Purpose
    Expression vector of mouse spermatogenesis associated 33 (Spata33) tagged with PA at C-terminus, CAG promoter, rabbit globin poly(A) signal. I83A mutation is introduced in Spata33.
    Depositor
    Masahito Ikawa
    Article
    Miyata et al Proc Natl Acad Sci U S A. 2021 Aug 31;11
    Insert
    spermatogenesis associated 33 (Spata33 Mouse)
    Tags
    PA
    Expression
    Mammalian
    Mutation
    Changed Isoleucine 83 to Alanine
    Available Since
    Jan. 25, 2022
    Availability
    Academic Institutions and Nonprofits only
    179132-plasmid-map-sequence-id-352797

    Enlarge

  26. pFAB1907

    Plasmid
    #47857
    Purpose
    BIOFAB reporter plasmid for measuring BBa_B1006 U10 termination efficiency
    Depositor
    Drew Endy
    Article
    Mutalik et al Nat Methods. 2013 Apr;10(4):354-60. doi:
    Insert
    BBa_B1006 U10
    Use
    Synthetic Biology
    Expression
    Bacterial
    Mutation
    Added two extra U at the end of Bba_1006 poly-U t…
    Promoter
    pLTetO12
    Available Since
    Dec. 3, 2013
    Availability
    Academic Institutions and Nonprofits only
    47857-plasmid-map-sequence-id-68389

    Enlarge

  27. pTEF:ATP

    Plasmid
    #92179
    Purpose
    HO-TEF1p-ATeam1.03-KanMX4-HO. Codon optimized for yeast version of the ATeam1.03 ATP FRET sensor (originally from Imamura et al. PNAS, 2009 ), expressed via the TEF1 promoter. HO locus integration.
    Depositor
    Matthias Heinemann
    Article
    Papagiannakis et al Mol Cell. 2017 Jan 19;65(2):285-295. doi
    Insert
    ATeam1.03 codon optimized for yeast (Saccharomyces cerevisiae)
    Use
    Integration and expression into yeast.
    Tags
    Yeast codon optimized mseCFP (donor) and cp173-mV…
    Expression
    Yeast
    Promoter
    TEF1p
    Available Since
    June 26, 2017
    Availability
    Academic Institutions and Nonprofits only
    92179-plasmid-map-sequence-id-181610

    Enlarge

  28. pCAG-hPPP3CA-FLAG

    Plasmid
    #179134
    Purpose
    Expression vector of human protein phosphatase 3, catalytic subunit, alpha isoform (PPP3CA) tagged with FLAG at C-terminus, CAG promoter, rabbit globin poly(A) signal.
    Depositor
    Masahito Ikawa
    Article
    Miyata et al Proc Natl Acad Sci U S A. 2021 Aug 31;11
    Insert
    protein phosphatase 3, catalytic subunit, alpha isoform (PPP3CA Human)
    Tags
    FLAG
    Expression
    Mammalian
    Available Since
    Jan. 25, 2022
    Availability
    Academic Institutions and Nonprofits only
    179134-plasmid-map-sequence-id-352802

    Enlarge

  30. pENTR221-BNT162b2ΔUTR

    Plasmid
    #171214
    Purpose
    Gateway entry vector containing the BNT162b2/tozinameran cDNA sequence lacking the original 5' and 3' UTRs and the poly(A) tail. The original Kozak sequence has also been altered.
    Depositor
    Liang Wei Wang
    Article
    Low et al medRxiv 2021.04.27.21256151
    Insert
    BNT162b2ΔUTR
    Use
    Gateway cloning
    Mutation
    Kozak sequence changed from CCCGCCACC to GGCTGCAC…
    Available Since
    June 30, 2021
    Availability
    Academic Institutions and Nonprofits only
    171214-plasmid-map-sequence-id-336476

    Enlarge

  36. pSLQ10871

    Plasmid
    #183962
    Purpose
    U6-SKSKSO(crRNA array)-CAG-dCas12a(WT)-miniVPR
    Depositor
    Stanley Qi
    Article
    Guo et al Nat Cell Biol. 2022 Apr;24(4):590-600. d
    Inserts
    dCas12a(wildtype)
    poly-crRNA array targeting Sox2-Klf4-Oct4
    Use
    CRISPR
    Tags
    HA
    Expression
    Mammalian
    Mutation
    D832A
    Promoter
    CAG and U6
    Available Since
    June 1, 2022
    Availability
    Academic Institutions and Nonprofits only
    183962-plasmid-map-sequence-id-363117

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Showing: 561 - 600 of 1236 results