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  4. pCAG-mSpata33 (I81A)-PA

    Plasmid
    #179131
    Purpose
    Expression vector of mouse spermatogenesis associated 33 (Spata33) tagged with PA at C-terminus, CAG promoter, rabbit globin poly(A) signal. I81A mutation is introduced in Spata33.
    Depositor
    Masahito Ikawa
    Article
    Miyata et al Proc Natl Acad Sci U S A. 2021 Aug 31;11
    Insert
    spermatogenesis associated 33 (Spata33 Mouse)
    Use
    Tags
    PA
    Expression
    Mammalian
    Mutation
    Changed Isoleucine 81 to Alanine
    Promoter
    Available since
    Jan. 25, 2022
    Availability
    Academic Institutions and Nonprofits only
    179131-plasmid-map-sequence-id-352818

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  5. pCAG-mSpata33 (P79A)-PA

    Plasmid
    #179130
    Purpose
    Expression vector of mouse spermatogenesis associated 33 (Spata33) tagged with PA at C-terminus, CAG promoter, rabbit globin poly(A) signal. P79A mutation is introduced in Spata33.
    Depositor
    Masahito Ikawa
    Article
    Miyata et al Proc Natl Acad Sci U S A. 2021 Aug 31;11
    Insert
    spermatogenesis associated 33 (Spata33 Mouse)
    Use
    Tags
    PA
    Expression
    Mammalian
    Mutation
    Changed Proline 79 to Alanine
    Promoter
    Available since
    Jan. 25, 2022
    Availability
    Academic Institutions and Nonprofits only
    179130-plasmid-map-sequence-id-352804

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  6. pCAG-mSpata33 (I83A)-PA

    Plasmid
    #179132
    Purpose
    Expression vector of mouse spermatogenesis associated 33 (Spata33) tagged with PA at C-terminus, CAG promoter, rabbit globin poly(A) signal. I83A mutation is introduced in Spata33.
    Depositor
    Masahito Ikawa
    Article
    Miyata et al Proc Natl Acad Sci U S A. 2021 Aug 31;11
    Insert
    spermatogenesis associated 33 (Spata33 Mouse)
    Use
    Tags
    PA
    Expression
    Mammalian
    Mutation
    Changed Isoleucine 83 to Alanine
    Promoter
    Available since
    Jan. 25, 2022
    Availability
    Academic Institutions and Nonprofits only
    179132-plasmid-map-sequence-id-352797

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  13. pFAB1907

    Plasmid
    #47857
    Purpose
    BIOFAB reporter plasmid for measuring BBa_B1006 U10 termination efficiency
    Depositor
    Drew Endy
    Article
    Mutalik et al Nat Methods. 2013 Apr;10(4):354-60. doi:
    Insert
    BBa_B1006 U10
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Added two extra U at the end of Bba_1006 poly-U t…
    Promoter
    pLTetO12
    Available since
    Dec. 3, 2013
    Availability
    Academic Institutions and Nonprofits only
    47857-plasmid-map-sequence-id-68389

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  15. pTEF:ATP

    Plasmid
    #92179
    Purpose
    HO-TEF1p-ATeam1.03-KanMX4-HO. Codon optimized for yeast version of the ATeam1.03 ATP FRET sensor (originally from Imamura et al. PNAS, 2009 ), expressed via the TEF1 promoter. HO locus integration.
    Depositor
    Matthias Heinemann
    Article
    Papagiannakis et al Mol Cell. 2017 Jan 19;65(2):285-295. doi
    Insert
    ATeam1.03 codon optimized for yeast (Saccharomyces cerevisiae)
    Use
    Integration and expression into yeast.
    Tags
    Yeast codon optimized mseCFP (donor) and cp173-mV…
    Expression
    Yeast
    Mutation
    Promoter
    TEF1p
    Available since
    June 26, 2017
    Availability
    Academic Institutions and Nonprofits only
    92179-plasmid-map-sequence-id-181610

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  17. pCAG-mPpp3cc-FLAG-6XHIS

    Plasmid
    #179160
    Purpose
    Expression vector of mouse protein phosphatase 3, catalytic subunit, gamma isoform (Ppp3cc) tagged with FLAG and 6X HIS at C-terminus, CAG promoter, rabbit globin poly(A) signal.
    Depositor
    Masahito Ikawa
    Article
    Miyata et al Science. 2015 Oct 23;350(6259):442-5. do
    Insert
    protein phosphatase 3, catalytic subunit, gamma isoform (Ppp3cc Mouse)
    Use
    Tags
    6XHIS and FLAG
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Jan. 25, 2022
    Availability
    Academic Institutions and Nonprofits only
    179160-plasmid-map-sequence-id-352799

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  18. pCAG-hPPP3CA-FLAG

    Plasmid
    #179134
    Purpose
    Expression vector of human protein phosphatase 3, catalytic subunit, alpha isoform (PPP3CA) tagged with FLAG at C-terminus, CAG promoter, rabbit globin poly(A) signal.
    Depositor
    Masahito Ikawa
    Article
    Miyata et al Proc Natl Acad Sci U S A. 2021 Aug 31;11
    Insert
    protein phosphatase 3, catalytic subunit, alpha isoform (PPP3CA Human)
    Use
    Tags
    FLAG
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Jan. 25, 2022
    Availability
    Academic Institutions and Nonprofits only
    179134-plasmid-map-sequence-id-352802

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  20. pAaePUb::Cas9-2A-Neo

    Plasmid
    #176680
    Purpose
    Expression of Drosophila codon optimized spCas9 under Ae. aegypti Polyubiquitin promoter (AAEL003888)
    Depositor
    Norbert Perrimon
    Article
    Viswanatha et al Nat Commun. 2021 Nov 24;12(1):6825. doi:
    Insert
    spCas9; NeoR (aminoglycoside phosphotransferase from Tn5)
    Use
    Crispr
    Tags
    Expression
    Insect
    Mutation
    Drosophila codon optimized
    Promoter
    Ae. aegypti poly-ubiquitin (AAEL003888)
    Available since
    Nov. 24, 2021
    Availability
    Academic Institutions and Nonprofits only
    176680-plasmid-map-sequence-id-348671

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  24. p2R3a-nosT

    Plasmid
    #71272
    Purpose
    Entry clone containing nosT. Necessary to complete the transcriptional reporters by providing the poly-A tail. For use in plants and compatible with the MultiSite Gateway system
    Depositor
    Ari Pekka Mähönen
    Article
    Siligato et al Plant Physiol. 2015 Dec 7. pii: pp.01246
    Insert
    nopaline synthase terminator
    Use
    Gateway
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Dec. 7, 2015
    Availability
    Academic Institutions and Nonprofits only
    71272-plasmid-map-sequence-id-129823

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  25. p2R3a-3AT

    Plasmid
    #71273
    Purpose
    Entry clone containing 3AT. Necessary to complete the transcriptional reporters by providing the poly-A tail. For use in plants and compatible with the MultiSite Gateway system
    Depositor
    Ari Pekka Mähönen
    Article
    Siligato et al Plant Physiol. 2015 Dec 7. pii: pp.01246
    Insert
    T3A ribulose-1,5-bisphosphate carboxylase 3A subunit terminator
    Use
    Gateway
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Dec. 7, 2015
    Availability
    Academic Institutions and Nonprofits only
    71273-plasmid-map-sequence-id-129830

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  27. pENTR221-BNT162b2ΔUTR

    Plasmid
    #171214
    Purpose
    Gateway entry vector containing the BNT162b2/tozinameran cDNA sequence lacking the original 5' and 3' UTRs and the poly(A) tail. The original Kozak sequence has also been altered.
    Depositor
    Liang Wei Wang
    Article
    Low et al medRxiv 2021.04.27.21256151
    Insert
    BNT162b2ΔUTR
    Use
    Gateway cloning
    Tags
    Expression
    Mutation
    Kozak sequence changed from CCCGCCACC to GGCTGCAC…
    Promoter
    Available since
    June 30, 2021
    Availability
    Academic Institutions and Nonprofits only
    171214-plasmid-map-sequence-id-336476

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  31. pSLQ10871

    Plasmid
    #183962
    Purpose
    U6-SKSKSO(crRNA array)-CAG-dCas12a(WT)-miniVPR
    Depositor
    Stanley Qi
    Article
    Guo et al Nat Cell Biol. 2022 Apr;24(4):590-600. d
    Inserts
    dCas12a(wildtype)
    poly-crRNA array targeting Sox2-Klf4-Oct4
    Use
    CRISPR
    Tags
    HA
    Expression
    Mammalian
    Mutation
    D832A
    Promoter
    CAG and U6
    Available since
    June 1, 2022
    Availability
    Academic Institutions and Nonprofits only
    183962-plasmid-map-sequence-id-363117

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  34. G30, Cdc20-mCherry-AID tagging

    Plasmid
    #130270
    Purpose
    Tagging the Saccharomyces cerevisiae Cdc20 gene with a mCherry and auxin-degron tag, via homologous recombination
    Depositor
    Matthias Heinemann
    Article
    Ozsezen et al Cell Syst. 2019 Oct 4. pii: S2405-4712(1
    Insert
    Cdc20/mCherry-AID(71-114)-tCYC1-NatMX (CDC20 Budding Yeast, Synthetic)
    Use
    Tags
    AID(71-114) and mCherry
    Expression
    Yeast
    Mutation
    Promoter
    Native promoter
    Available since
    Sept. 11, 2019
    Availability
    Academic Institutions and Nonprofits only
    130270-plasmid-map-sequence-id-255828

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Showing: 561 - 600 of 1185 results