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sgRNA(MS2) cloning backbone

Plasmid

#61424

Purpose

sgRNA cloning backbone with MS2 loops at tetraloop and stemloop 2. Contains BbsI sites for insertion of spacer sequences.

Depositor

Feng Zhang

Publication

Konermann et al Nature. 2014 Dec 10. doi: 10.1038/nature

Type

Empty backbone

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available since

Dec. 15, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

66 Articles

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pDONR223-MAP4K4

Plasmid

#23486

Depositor

William Hahn, David Root

Publication

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

MAP4K4 (MAP4K4 Human)

Use

Gateway Cloning

Available since

July 30, 2010

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pCAG-ERT2CreERT2

Plasmid

#13777

Purpose

Mammalian expression of conditionally active form of Cre recombinase (activated in response to tamoxifen)

Depositor

Connie Cepko

Publication

Matsuda et al Proc Natl Acad Sci U S A. 2007 Jan 16;10

Insert

ERT2-Cre-ERT2 fusion protein

Use

Cre/Lox

Expression

Mammalian

Promoter

CAG

Available since

April 20, 2007

Availability

Academic Institutions and Nonprofits only

Cited by

59 Articles

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HA-p62

Plasmid

#28027

Purpose

Mammalian expression of HA tagged human p62

Depositor

Qing Zhong

Publication

Fan et al Autophagy. 2010 Jul 22. 6(5):.

Insert

p62 (SQSTM1 Human)

Tags

HA

Expression

Mammalian

Available since

Feb. 17, 2011

Availability

Academic Institutions and Nonprofits only

Cited by

56 Articles

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GFP-HSPA8

Plasmid

#121161

Purpose

Mammalian expression of GFP tagged HSPA8

Depositor

Christine Mayr

Publication

Ma et al Cell. 2018 Oct 30. pii: S0092-8674(18)31

Insert

HSPA8 (HSPA8 Human)

Expression

Mammalian

Available since

Feb. 7, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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BJ5183 cells

Bacterial strain

#16398

Depositor

Bert Vogelstein

Publication

He et al Proc Natl Acad Sci U S A. 1998 Mar 3. 95

Bacterial resistance

Streptomycin

Species

Escherichia coli

Available since

June 11, 2008

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles
pU6-(BbsI)_CBh-Cas9-T2A-mCherry

Plasmid

#64324

Purpose

Expression vector for sgRNAs cloned into the BbsI sites and for expression of Cas9 linked to mCherry via a T2A peptide

Depositor

Ralf Kuehn

Publication

Chu et al Nat Biotechnol. 2015 Mar 24. doi: 10.103

Inserts

Cas9
sgRNA cassette

Use

CRISPR

Tags

3xFLAG, NLS, and T2A-mCherry

Expression

Mammalian

Promoter

CBh and U6

Available since

Sept. 2, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

114 Articles

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pB-CAGGS-dCas9-KRAB-MeCP2

Plasmid

#110824

Purpose

PiggyBac compatible plasmid expressing dCas9-KRAB-MeCP2

Depositor

Alejandro Chavez, George Church

Publication

Yeo et al Nat Methods. 2018 Jul 16. pii: 10.1038/s

Insert

dCas9-KRAB-MeCP2

Expression

Mammalian

Mutation

Cas9 mutations to make dCas9=D10A+D839A+H840A+N86…

Promoter

CAGGS

Available since

July 17, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

19 Articles

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pCXWB-EBNA1

Plasmid

#37624

Purpose

Non-replicating episomal expression of EBNA1, which enhances transfection efficiency and expression from episomal plasmids

Depositor

Shinya Yamanaka

Publication

Okita et al Stem Cells. 2012 Nov 29. doi: 10.1002/st

Insert

EBNA-1 (EBNA-1 human herpesvirus 4)

Expression

Mammalian

Promoter

CAG

Available since

Dec. 3, 2012

Availability

Academic Institutions and Nonprofits only

Cited by

72 Articles

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R619-M66-303: CMV51p> HCoV-OC43 S-2P-T4f-3C-His8-Strep2x2

Plasmid

#166015

Purpose

mammalian expression of HCoV-OC43 soluble spike trimer protein

Depositor

Dominic Esposito

Publication

Esposito et al Protein Expr Purif. 2020 Jun 4;174:10568

Insert

HCoV-OC43 S (spike)

Tags

3C-His8-Strep2x2

Expression

Mammalian

Promoter

CMV51

Available since

March 11, 2021

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pRK5-HA-Ubiquitin-K48R

Plasmid

#17604

Purpose

Mammalian expression of HA tagged ubiquitin K48R

Depositor

Ted Dawson

Publication

Lim et al J Neurosci. 2005 Feb 23. 25(8):2002-9.

Insert

Ubiquitin C (UBC Human)

Tags

HA

Expression

Mammalian

Mutation

K48R (expected to disrupt G76-K48 ubiquitin chain…

Available since

March 31, 2008

Availability

Academic Institutions and Nonprofits only

Cited by

52 Articles

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pCMV-ABE7.10

Plasmid

#102919

Purpose

expresses ABE7.10 in mammalian cells

Depositor

David Liu

Publication

Gaudelli et al Nature. 2017 Nov 23;551(7681):464-471. d

Insert

ABE7.10

Tags

NLS

Expression

Mammalian

Mutation

see manuscript

Promoter

CMV

Available since

Nov. 27, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

40 Articles

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pShuttle-CMV

Plasmid

#16403

Purpose

Shuttle vector for use in AdEasy System. Used for expression of transgenes under a CMV promoter when no GFP tracer is desired.

Depositor

Bert Vogelstein

Publication

He et al Proc Natl Acad Sci U S A. 1998 Mar 3. 95

Type

Empty backbone

Use

Adenoviral

Expression

Mammalian

Available since

June 11, 2008

Availability

Academic Institutions and Nonprofits only

Cited by

24 Articles

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pCSN067

Plasmid

#101748

Purpose

Single copy yeast plasmid expressing Cpf1 from Lachnospiraceae bacterium ND2006 (LbCpf1), codon optimized for expression in Saccharomyces cerevisiae.

Depositor

Rene Verwaal

Publication

Verwaal et al Yeast. 2017 Sep 8. doi: 10.1002/yea.3278

Inserts

Cpf1 from Lachnospiraceae bacterium ND2006 (LbCpf1) codon optimized for expression in S. cerevisiae.
KanMX marker expression cassette.

Use

CRISPR

Tags

SV40 NLS

Expression

Yeast

Promoter

Heterologous TEF1 promoter from A. gossypii. and …

Available since

Dec. 8, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles

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pOPINK

Plasmid

#41143

Depositor

Ray Owens

Insert

GST

Expression

Bacterial

Available since

March 29, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles

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pmU6-gRNA

Plasmid

#53187

Purpose

Expresses the S. pyogenes sgRNA from the mouse U6 promoter

Depositor

Charles Gersbach

Publication

Kabadi et al Nucleic Acids Res. 2014 Aug 13. pii: gku

Type

Empty backbone

Use

CRISPR

Expression

Mammalian

Promoter

mouse U6

Available since

June 25, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

27 Articles

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pCRIS-PITChv2-FBL

Plasmid

#63672

Purpose

PITCh donor vector for EGFP-2A-PuroR knock-in into human FBL locus

Depositor

Takashi Yamamoto

Publication

Sakuma et al Nat Protoc. 2016 Jan;11(1):118-33. doi:

Insert

EGFP-2A-PuroR

Use

CRISPR

Promoter

Promoterless

Available since

Dec. 18, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

17 Articles

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pET-28b-Cas9-His

Plasmid

#47327

Purpose

For in vitro expression and purification of Cas9 protein

Depositor

Alex Schier

Publication

Gagnon et al PLoS One. 2014 May 29;9(5):e98186. doi:

Insert

Cas9

Use

CRISPR

Tags

His

Expression

Bacterial

Available since

March 25, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

35 Articles

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R619-M89-303: CMV51p> HCoV-HKU1 S-2P-T4f-3C-His8-Strep2x2

Plasmid

#166014

Purpose

mammalian expression of HCoV-HKU1 soluble spike trimer protein

Depositor

Dominic Esposito

Publication

Esposito et al Protein Expr Purif. 2020 Jun 4;174:10568

Insert

HCoV-HKU1 S (spike)

Tags

3C-His8-Strep2x2

Expression

Mammalian

Promoter

CMV51

Available since

March 16, 2021

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pAAV_hSyn1-SIO-stGtACR2-FusionRed (AAV1)

Viral prep

#105677-AAV1

Purpose

Ready-to-use AAV1 particles produced from pAAV_hSyn1-SIO-stGtACR2-FusionRed (#105677). In addition to the viral particles, you will also receive purified pAAV_hSyn1-SIO-stGtACR2-FusionRed plasmid DNA. Synapsin-driven, Cre-dependent, soma-targeted anion-conducting channelrhodopsin fused to FusionRed for optogenetic inhibition. These AAV preparations are suitable purity for injection into animals.

Depositor

Ofer Yizhar

Publication

Mahn et al Nat Commun. 2018 Oct 8;9(1):4125. doi: 1

Promoter

Syn

Tags

FusionRed (Cre-dependent)

Available since

Aug. 17, 2018

Availability

Academic Institutions and Nonprofits only