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Plasmid#169656PurposeTier-3 vector for stable stable integration of up to three cassettes via Piggy Bac transposase including a mTagBFP2 marker and BlastR resistance gene (5'ITR-A1-pA::A2-pA::PRPBSA-mTagBFP2-p2A-BlastR-pA-3'ITR)DepositorInsertPRPBSA-driven mTagBFP2 and BlastR expression
UseTagsExpressionMammalianMutationPromoterPRPBSAAvailable sinceJune 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLX208-nfBFP
Plasmid#171013PurposeEctopic expression of nonfluorescent BFPDepositorInsertuntagged nfBFP
UseLentiviralTagsExpressionMammalianMutationBFP[H66A]PromoterEf1-alphaAvailable sinceAug. 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
SIN40C.SFFV.mTagBFP2.miR30n
Plasmid#169281PurposeConstitutive overexpression of miRNA or shRNA with mTagBFP2 as reporter fluorescent proteinDepositorTypeEmpty backboneUseLentiviral and RNAiTagsExpressionMutationPromoterAvailable sinceJuly 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
pQLinkHD_EBFP2-ECFP
Plasmid#118854Purposefluorescent protein expression with an N-terminal His-tagDepositorInsertEBFP2-ECFP
UseTagsHis-tagExpressionBacterialMutationEBFP1.2-I128V/V150I/ D155V/V224R and TGAGGGGLG be…PromoterAvailable sinceDec. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
pQLinkHD_EBFP2-mTFP1
Plasmid#118855Purposefluorescent protein expression with an N-terminal His-tagDepositorInsertEBFP2-mTFP1
UseTagsHis-tagExpressionBacterialMutationEBFP1.2-I128V/V150I/ D155V/V224R and TGAGGGGLG be…PromoterAvailable sinceDec. 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
YCe2034 HC_Kan_mTagBFP2_p9
Plasmid#100689Purposepart designed to occupy position 9 of EMMA. Functional category: Fluorescent markerDepositorInsertmTAGBFP2
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceNov. 17, 2017AvailabilityAcademic Institutions and Nonprofits only -
mTagBFP2-Myotilin-C-14
Plasmid#55316PurposeLocalization: Cytoskeleton, Excitation: 399, Emission: 456DepositorInsertMyotilin (MYOT Human)
UseTagsmTagBFP2ExpressionMammalianMutationPromoterCMVAvailable sinceOct. 22, 2014AvailabilityIndustry, Academic Institutions, and Nonprofits -
MSCV-Cas9-2A-BFP
Plasmid#164662PurposeRetroviral introduction of Cas9 into mammalian cell line coupled to expression of blue fluorescent proteinDepositorInsertsCas9
BFP
UseCRISPR and RetroviralTagsExpressionMammalianMutationPromoterMSSV_LTRAvailable sinceMarch 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
pEF1α-Cre-2A-mTagBFP2
Plasmid#207746PurposeExpression of the Cre recombinase linked to a mTagBFP2 fluorescent markerDepositorInsertCre-PGK-mTagBFP2
UseCre/LoxTagsExpressionMammalianMutationPromoterEF1alphaAvailable sinceNov. 15, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits -
pHAGE-EF1aL-TagBFP-W
Plasmid#126687PurposeConstitutive, ubiquitous blue fluorescence protein (BFP) expression.DepositorInsertTagBFP
UseLentiviralTagsExpressionMutationPromoterEf1aLAvailable sinceJune 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pTag-BFP-C-h-Rab11a
Plasmid#79805PurposeExpress TagBFP labeled human Rab11a (GTPase located on recycling endosomal membranes).DepositorInsertRab11a (RAB11A Human)
UseTagsTagBFPExpressionMammalianMutationPromoterCMVAvailable sinceJuly 22, 2016AvailabilityIndustry, Academic Institutions, and Nonprofits -
pTBL3478 PB-PEmax-mTagBFP2
Plasmid#226664PurposePiggyBac cargo vector encoding PEmax and mTagBFP2DepositorInsertsPEmax
mTagBFP2
UseCRISPRTagsExpressionMammalianMutationPromoterCAG and UBCAvailable sinceOct. 28, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLV-EBFP2-nuc
Plasmid#36085DepositorTypeEmpty backboneUseLentiviralTagsNLSExpressionMammalianMutationReplace EYFP of the vector pEYFP-Nuc from Clontec…PromoterAvailable sinceJune 1, 2012AvailabilityAcademic Institutions and Nonprofits only -
pR26 iBFP Dest
Plasmid#74284PurposeGene targeting vector for the mouse Rosa26 locus, including a loxP flanked stop cassette and BFP, for gateway cloningDepositorInsertRosa26 5-homology region
UseTagsExpressionMutationPromoterAvailable sinceMay 2, 2016AvailabilityAcademic Institutions and Nonprofits only -
pLenti_rtTA-P2A-BFP_HygroR
Plasmid#186738PurposertTA-P2A-BFP expression construct cloned into lentiviral backboneDepositorInsertrtTA
UseLentiviralTagsP2A-BFPExpressionMammalianMutationPromoterEF1aAvailable sinceApril 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
BFP-Sec61 beta
Plasmid#49154Purposeexpression of Sec61 beta fused to BFP, used as a general ER markerDepositorInsertSec61 beta (SEC61B Human)
UseTagsBFPExpressionMammalianMutationPromoterCMVAvailable sinceMay 29, 2014AvailabilityAcademic Institutions and Nonprofits only -
lentiGuide-Hygro-mTagBFP2
Plasmid#99374PurposeExpresses S. pyogenes CRISPR chimeric RNA element with customizable sgRNA from U6 promoter and hygromycin resistance marker with 2A mTagBFP2 from EF-1a promoter. 3rd generation lentiviral backbone.DepositorInsertsS. pyogenes sgRNA cassette
Hygro-P2A-mTagBFP2
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterEF-1a and hU6Available sinceAug. 18, 2017AvailabilityAcademic Institutions and Nonprofits only -
pLKO.1 - TRC mTagBFP2
Plasmid#191566PurposeExpresses an shRNA and mTagBFP2DepositorTypeEmpty backboneUseLentiviral and RNAiTagsExpressionMammalianMutationPromoterU6 for shRNAAvailable sinceOct. 17, 2022AvailabilityAcademic Institutions and Nonprofits only -
pHR_TRE3GS-->mCherry_PGK-->BFP
Plasmid#162231PurposeLentiviral vector for the expression of TRE3GS-->mCherry_PGK-->BFP in mammalian cellsDepositorInsertmCherry and BFP
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceJune 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
lenti-mito-mTagBFP2
Plasmid#216130PurposeLentiviral vector for expressing mTagBFP2 localizing to mitochondria.DepositorInsertEF1a-COX8AmitochondrialLocalizationSignal-mTagBFP2-WPRE
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceApril 1, 2024AvailabilityAcademic Institutions and Nonprofits only