We narrowed to 23,137 results for: pET

1. pET21a - Ec-ClpX

   Plasmid

   #111516

   Purpose

   Expresses E. coli ClpX with a C-terminal His tag

   Depositor

   Judy Lieberman

   Article

   Dotiwala et al Cell. 2017 Nov 16;171(5):1125-1137.e11.

   Insert

   ClpX
   

   Tags

   His tag

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   June 25, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pET-2(KW)2-eGFP

   Plasmid

   #112736

   Purpose

   Express 2(KW)2-eGFP in E. coli

   Depositor

   Kyubong Jo

   Article

   Lee et al Nucleic Acids Res. 2016 Jan 8;44(1):e6.

   Insert

   2(KW)2-eGFP
   

   Tags

   6xHis

   Expression

   Bacterial

   Promoter

   T7 Promoter

   Available Since

   July 30, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pET28-hXRCC1

   Plasmid

   #70759

   Purpose

   bacterial expression of human XRCC1

   Depositor

   Primo Schaer

   Article

   Schuermann et al Nucleic Acids Res. 2016 Jan 4. pii: gkv1

   Insert

   XRCC1 (XRCC1 Human)
   

   Tags

   6-HIS

   Expression

   Bacterial

   Promoter

   T7/lac operon

   Available Since

   Jan. 14, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. pET21c(+)-mtLeuRS

   Plasmid

   #31079

   Depositor

   Linda Spremulli

   Article

   Bullard et al Biochim Biophys Acta. 2000 Feb 29. 1490(

   Insert

   Mature form human mitochondrial leucyl-tRNA synthetase (LARS2 Human)
   

   Tags

   His

   Expression

   Bacterial

   Mutation

   Includes coding sequence for the mature form. Nt …

   Available Since

   Sept. 19, 2011

   Availability

   Academic Institutions and Nonprofits only

5. Nampt-PET28a

   Plasmid

   #25630

   Depositor

   Cynthia Wolberger

   Article

   Wang et al Nat Struct Mol Biol. 2006 Jul . 13(7):66

   Insert

   Nampt (Nampt Mouse)
   

   Tags

   His6

   Expression

   Bacterial

   Available Since

   Aug. 9, 2010

   Availability

   Academic Institutions and Nonprofits only

6. pET41a iGluu

   Plasmid

   #119834

   Purpose

   Production of recombinant iGluSnFR S72T ultrafast variant, a single-wavelength glutamate indicator, in E. coli

   Depositor

   Katalin Torok

   Article

   Helassa et al Proc Natl Acad Sci U S A. 2018 May 7. pi

   Insert

   iGluu
   

   Tags

   GST

   Expression

   Bacterial

   Mutation

   S72T

   Promoter

   T7

   Available Since

   April 1, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pET28a-Ec.coaA

   Plasmid

   #50386

   Purpose

   Heterologous expression of E. coli CoA biosynthetic enzyme, PanK (pantothenate kinase), induced with IPTG. Contain 6xHis-tag for nickel affinity purification.

   Depositor

   Erick Strauss

   Article

   Brand et al J Biol Chem. 2005 May 27;280(21):20185-8

   Insert

   PanK
   

   Tags

   6XHis-tag

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   March 13, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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8. pET32-MCM2/7

   Plasmid

   #116949

   Purpose

   Expresses human MCM2 and human MCM7

   Depositor

   James Chong

   Article

   Hesketh et al J Biol Chem. 2015 Mar 20;290(12):7973-9.

   Inserts

   Human MCM7 (MCM7 Human)
   Human MCM2 (MCM2 Human)
   

   Tags

   Thioredoxin, His6

   Expression

   Bacterial

   Available Since

   Oct. 24, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pET42b-HF-BE3

   Plasmid

   #87438

   Purpose

   Expresses HF-BE3-NLS with an N-terminal His Tag (His6) for bacterial expression

   Depositor

   David Liu

   Article

   Rees et al Nat Commun. 2017 Jun 6;8:15790. doi: 10.

   Insert

   HF-BE3 (Apobec1 Bacillus phage PBS2, Human, Rat)
   

   Tags

   His6

   Expression

   Bacterial

   Available Since

   June 13, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. pET14-GFP15_Encap

    Plasmid

    #86407

    Purpose

    Expresses sfGFP tagged with a 15 amino acid CLP and encapsulin as an operon

    Depositor

    David Savage

    Article

    Cassidy-Amstutz et al Biochemistry. 2016 Jun 21;55(24):3461-8.

    Inserts

    sfGFP with a 15aa CLP tag
    Encapsulin
    

    Tags

    6x Histidine

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    April 26, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. pET_RGG-RGG

    Plasmid

    #124941

    Purpose

    Expresion of RGG-RGG

    Depositor

    Benjamin Schuster, Matthew Good, Daniel Hammer

    Article

    Schuster et al Nat Commun. 2018 Jul 30;9(1):2985. doi:

    Insert

    RGG-RGG (using LAF-1 RGG domain) (laf-1 Nematode)
    

    Tags

    His

    Expression

    Bacterial

    Available Since

    May 29, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pET26b-Granzyme A

    Plasmid

    #8823

    Depositor

    Judy Lieberman

    Article

    Beresford et al Proc Natl Acad Sci U S A 1997 Aug 19;94(

    Insert

    Human Granzyme A (GZMA Human)
    

    Tags

    (His)6 and Enterokinase site

    Expression

    Bacterial

    Mutation

    it mature enzyme.

    Available Since

    Sept. 26, 2005

    Availability

    Academic Institutions and Nonprofits only

    

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13. pET22b-csgA

    Plasmid

    #89581

    Purpose

    Express csgA curli protein with GS linker and Histaq

    Depositor

    Urartu Seker

    Article

    Abdelwahab et al Langmuir. 2017 Apr 7. doi: 10.1021/acs.l

    Insert

    csgA (csgA )
    

    Tags

    3xGGSG linker - TEV site - 3xGGSG linker and 6xHi…

    Expression

    Bacterial

    Available Since

    May 19, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. pET11a-hRanGAP1

    Plasmid

    #50941

    Purpose

    expression of full length untagged human RanGAP1 in E.coli

    Depositor

    Frauke Melchior

    Article

    Werner et al Mol Cell. 2012 May 11;46(3):287-98. doi:

    Insert

    RanGTPase activating protein 1 (RANGAP1 Human)
    

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    May 30, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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15. pET-IG-mSA

    Plasmid

    #80706

    Purpose

    Expresses monomeric streptavidin (mSA) in bacterial cells

    Depositor

    Matthieu Sainlos

    Article

    Chamma et al Nat Commun. 2016 Mar 16;7:10773. doi: 10

    Insert

    Monomeric streptavidin
    

    Tags

    10xHis tag and TEV site

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Sept. 14, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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16. pET3a-Ub-K63R

    Plasmid

    #18898

    Depositor

    Cecile Pickart

    Article

    Raasi et al Methods Mol Biol. 2005;301:47-55. doi: 1

    Insert

    Ubiquitin-K63R (UBC Human)
    

    Expression

    Bacterial

    Mutation

    Lysine 63 is replaced with a Argnine residue

    Available Since

    Sept. 10, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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17. pET28a-CPDSalI

    Plasmid

    #38252

    Depositor

    Aimee Shen, Matthew Bogyo

    Article

    Shen et al PLoS One. 2009 Dec 2;4(12):e8119.

    Type

    Empty backbone

    Tags

    6xHis and Vibrio cholerae MARTX toxin cysteine pr…

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Aug. 2, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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18. Ypet-PBD

    Plasmid

    #105290

    Purpose

    To measure Rac or Cdc42 activity by binding and FRET analysis

    Depositor

    Kenneth Yamada

    Article

    Petrie et al J Cell Biol. 2012 Apr 30;197(3):439-55.

    Insert

    Pak1 (PAK1 Human)
    

    Tags

    Ypet

    Expression

    Mammalian

    Mutation

    aa encoding 65-150

    Available Since

    Feb. 21, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. pET28a-HsSENP3

    Plasmid

    #71467

    Purpose

    Encodes human full length SENP3. Suitable for in vitro transcription/translation (T7).

    Depositor

    Jorge Eduardo Azevedo

    Article

    Mendes et al Biochim Biophys Acta. 2015 Oct 29. pii:

    Insert

    SUMO1/sentrin specific peptidase 3 (SENP3 Human)
    

    Tags

    Hexahistidine

    Expression

    Bacterial

    Mutation

    Gene optimised for E. coli expression (Genscript)

    Promoter

    T7

    Available Since

    Dec. 10, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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20. pET28-MKH8SUMO

    Plasmid

    #79526

    Purpose

    Bacterial expression plasmid used for T7 promoter driven expression of recombinant proteins with N-terminal 8X His, thrombin cleavage site, SUMO, and TEV cleavage site.

    Depositor

    Cheryl Arrowsmith

    Type

    Empty backbone

    Tags

    8xHis-Thrombin-SUMO-TEV and TEV cleavage site

    Expression

    Bacterial

    Available Since

    June 16, 2016

    Availability

    Industry, Academic Institutions, and Nonprofits

21. pET29b-pduCDE

    Plasmid

    #110138

    Purpose

    Expresses B12-dependent propanediol dehydratase in E. coli BL21(DE3)

    Depositor

    Emily Balskus

    Article

    Levin et al Biochemistry. 2018 Mar 16. doi: 10.1021/

    Insert

    pduCDE (pduE, pduD, pduC Klebsiella oxytoca ATCC 8724)
    

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    May 22, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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22. pCEP4YPet-MAMM

    Plasmid

    #14032

    Depositor

    Patrick Daugherty

    Article

    Nguyen et al Nat Biotechnol. 2005 Mar;23(3):355-60. d

    Insert

    YPet
    

    Expression

    Mammalian

    Mutation

    Mammalian optimized YPet

    Available Since

    Feb. 14, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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23. BglB in pET29b(+)

    Plasmid

    #55773

    Purpose

    Expresses beta-glucosidase

    Depositor

    Justin Siegel

    Article

    Carlin et al PLoS One. 2016 Jan 27;11(1):e0147596. do

    Insert

    Beta-glucosidase B (bglB Synthetic)
    

    Tags

    6xHis

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    July 30, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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24. pET28a-TRBP

    Plasmid

    #50351

    Purpose

    bacterial expression of TRBP

    Depositor

    Kumiko Ui-Tei

    Article

    Takahashi et al PLoS One. 2013 May 2;8(5):e63434. doi: 1

    Insert

    TRBP (TARBP2 Human)
    

    Tags

    His and Myc

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Jan. 15, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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25. pET28a-SpySrtA

    Plasmid

    #51139

    Purpose

    expression plasmid for N-terminal his tagged S.pyogenes SrtA

    Depositor

    Hidde Ploegh

    Article

    Guimaraes et al Nat Protoc. 2013 Sep;8(9):1787-99. doi:

    Insert

    S.pyogenes SrtA
    

    Tags

    6x His

    Expression

    Bacterial

    Mutation

    Deleted amino acids 1-80

    Promoter

    T7

    Available Since

    Feb. 14, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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26. pET28a-Rx3(A7)

    Plasmid

    #35525

    Depositor

    Robert Cohen

    Article

    Sims et al Nat Methods. 2012 Feb 5. doi: 10.1038/nm

    Insert

    His6-Rx3(A7)
    

    Tags

    His6

    Expression

    Bacterial

    Mutation

    Changed G2 to P

    Promoter

    T7

    Available Since

    April 5, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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27. pET21a AHU

    Plasmid

    #26640

    Depositor

    Phoebe Rice

    Article

    Swinger et al EMBO J. 2003 Jul 15. 22(14):3749-60.

    Insert

    AHU
    

    Expression

    Bacterial

    Available Since

    Oct. 22, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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28. pET28a-MSP1D1delltaH4

    Plasmid

    #71713

    Purpose

    Membrane scaffold protein (MSP), helix 4 deletion

    Depositor

    Gerhard Wagner

    Article

    Hagn et al J Am Chem Soc. 2013 Feb 6;135(5):1919-25

    Insert

    Apolipoprotein A1 (APOA1 Human)
    

    Tags

    6xHis

    Expression

    Bacterial

    Available Since

    March 10, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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29. pET15b-mISG15(GG)

    Plasmid

    #12448

    Depositor

    Dong-Er Zhang

    Article

    Kim et al Methods Enzymol. 2005;398:491-9. doi: 10

    Insert

    C-terminal processed form of ISG15
    

    Tags

    His

    Expression

    Bacterial

    Available Since

    Jan. 4, 2007

    Availability

    Academic Institutions and Nonprofits only

30. pET28a-PACT

    Plasmid

    #50352

    Purpose

    bacterial expression of PACT

    Depositor

    Kumiko Ui-Tei

    Article

    Takahashi et al PLoS One. 2013 May 2;8(5):e63434. doi: 1

    Insert

    PACT (PRKRA Human)
    

    Tags

    His and Myc

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Jan. 15, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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