We narrowed to 14,071 results for: cas9

1. pCSDest2-2XNLS-SpCas9-WT-NLS-3XHA-NLS-TALentry

   Plasmid

   #69232

   Purpose

   Wild type SpCas9 expression plasmid to clone TALEs through BbsI site (generates compatible overhangs with Acc65I and BamHI sites)

   Depositor

   Scot Wolfe

   Article

   Bolukbasi et al Nat Methods. 2015 Oct 19. doi: 10.1038/n

   Insert

   SpCas9
   

   Use

   CRISPR

   Tags

   2X NLS, BbsI TALE entry cassette, and NLS-3XHA-NL…

   Expression

   Mammalian

   Available Since

   Oct. 20, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCMV-SpCas9-VRQR(+R221K)-P2A-EGFP (LRF95)

   Plasmid

   #242666

   Purpose

   CMV promoter expression plasmid for human codon optimized SpCas9-VRQR(R221K)-BPNLS(SV40)-3xFLAG-P2A-EGFP

   Depositor

   Benjamin Kleinstiver

   Insert

   pCMV-SpCas9-VRQR(R221K)-BPNLS(SV40)-3xFLAG-P2A-EGFP
   

   Use

   CRISPR

   Tags

   BPNLS

   Expression

   Mammalian

   Mutation

   VRQR + R221K mutations in SpCas9(R221K/D1135V/G12…

   Promoter

   CMV and T7

   Available Since

   Oct. 9, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCMV-SpCas9-VRQR(+N394K)-P2A-EGFP (KAC956)

   Plasmid

   #242670

   Purpose

   CMV promoter expression plasmid for human codon optimized SpCas9-VRQR(N394K)-BPNLS(SV40)-3xFLAG-P2A-EGFP

   Depositor

   Benjamin Kleinstiver

   Insert

   pCMV-SpCas9-VRQR(N394K)-BPNLS(SV40)-3xFLAG-P2A-EGFP
   

   Use

   CRISPR

   Tags

   BPNLS

   Expression

   Mammalian

   Mutation

   VRQR + N394K mutations in SpCas9(N394K/D1135V/G12…

   Promoter

   CMV and T7

   Available Since

   Oct. 9, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCMV-SpCas9-VRQR(+D269A)-P2A-EGFP (LRF64)

   Plasmid

   #242668

   Purpose

   CMV promoter expression plasmid for human codon optimized SpCas9-VRQR(D269A)-BPNLS(SV40)-3xFLAG-P2A-EGFP

   Depositor

   Benjamin Kleinstiver

   Insert

   pCMV-SpCas9-VRQR(D269A)-BPNLS(SV40)-3xFLAG-P2A-EGFP
   

   Use

   CRISPR

   Tags

   BPNLS

   Expression

   Mammalian

   Mutation

   VRQR + D269A mutations in SpCas9(D269A/D1135V/G12…

   Promoter

   CMV and T7

   Available Since

   Oct. 9, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCMV-SpCas9-VRQR(+G366R)-P2A-EGFP (KAC1026)

   Plasmid

   #242669

   Purpose

   CMV promoter expression plasmid for human codon optimized SpCas9-VRQR(G366R)-BPNLS(SV40)-3xFLAG-P2A-EGFP

   Depositor

   Benjamin Kleinstiver

   Insert

   pCMV-SpCas9-VRQR(G366R)-BPNLS(SV40)-3xFLAG-P2A-EGFP
   

   Use

   CRISPR

   Tags

   BPNLS

   Expression

   Mammalian

   Mutation

   VRQR + G366R mutations in SpCas9(G366R/D1135V/G12…

   Promoter

   CMV and T7

   Available Since

   Oct. 9, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCMV-SpCas9-VRQR(+N1286Q)-P2A-EGFP (LRF247)

   Plasmid

   #242677

   Purpose

   CMV promoter expression plasmid for human codon optimized SpCas9-VRQR(N1286Q)-BPNLS(SV40)-3xFLAG-P2A-EGFP

   Depositor

   Benjamin Kleinstiver

   Insert

   pCMV-SpCas9-VRQR(N1286Q)-BPNLS(SV40)-3xFLAG-P2A-EGFP
   

   Use

   CRISPR

   Tags

   BPNLS

   Expression

   Mammalian

   Mutation

   VRQR + N1286Q mutations in SpCas9(D1135V/G1218R/N…

   Promoter

   CMV and T7

   Available Since

   Oct. 9, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pCMV-SpCas9-VRQR(+G1104K)-P2A-EGFP (LRF54)

   Plasmid

   #242671

   Purpose

   CMV promoter expression plasmid for human codon optimized SpCas9-VRQR(G1140K)-BPNLS(SV40)-3xFLAG-P2A-EGFP

   Depositor

   Benjamin Kleinstiver

   Insert

   pCMV-SpCas9-VRQR(G1140K)-BPNLS(SV40)-3xFLAG-P2A-EGFP
   

   Use

   CRISPR

   Tags

   BPNLS

   Expression

   Mammalian

   Mutation

   VRQR + G1104K mutations in SpCas9(G1104K/D1135V/G…

   Promoter

   CMV and T7

   Available Since

   Oct. 9, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pCMV-SpCas9-VRQR(+A61R)-P2A-EGFP (KAC1030)

   Plasmid

   #242665

   Purpose

   CMV promoter expression plasmid for human codon optimized SpCas9-VRQR(A61R)-BPNLS(SV40)-3xFLAG-P2A-EGFP

   Depositor

   Benjamin Kleinstiver

   Insert

   pCMV-SpCas9-VRQR(A61R)-BPNLS(SV40)-3xFLAG-P2A-EGFP
   

   Use

   CRISPR

   Tags

   BPNLS

   Expression

   Mammalian

   Mutation

   VRQR + A61R mutations in SpCas9(A61R/D1135V/G1218…

   Promoter

   CMV and T7

   Available Since

   Oct. 9, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCMV-SpCas9-VRQR(+L244Q)-P2A-EGFP (LRF96)

   Plasmid

   #242667

   Purpose

   CMV promoter expression plasmid for human codon optimized SpCas9-VRQR(L244Q)-BPNLS(SV40)-3xFLAG-P2A-EGFP

   Depositor

   Benjamin Kleinstiver

   Insert

   pCMV-SpCas9-VRQR(L244Q)-BPNLS(SV40)-3xFLAG-P2A-EGFP
   

   Use

   CRISPR

   Tags

   BPNLS

   Expression

   Mammalian

   Mutation

   VRQR + L244Q mutations in SpCas9(L244Q/D1135V/G12…

   Promoter

   CMV and T7

   Available Since

   Oct. 9, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pCMV-SpCas9-VRQR(+N1317R)-P2A-EGFP (LRF53)

    Plasmid

    #242678

    Purpose

    CMV promoter expression plasmid for human codon optimized SpCas9-VRQR(N1317R)-BPNLS(SV40)-3xFLAG-P2A-EGFP

    Depositor

    Benjamin Kleinstiver

    Insert

    pCMV-SpCas9-VRQR(N1317R)-BPNLS(SV40)-3xFLAG-P2A-EGFP
    

    Use

    CRISPR

    Tags

    BPNLS

    Expression

    Mammalian

    Mutation

    VRQR + N1317R mutations in SpCas9(D1135V/G1218R/N…

    Promoter

    CMV and T7

    Available Since

    Oct. 9, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pCMV-SpCas9-VRQR(+A1285K)-P2A-EGFP (KAC1134)

    Plasmid

    #242676

    Purpose

    CMV promoter expression plasmid for human codon optimized SpCas9-VRQR(A1285K)-BPNLS(SV40)-3xFLAG-P2A-EGFP

    Depositor

    Benjamin Kleinstiver

    Insert

    pCMV-SpCas9-VRQR(A1285K)-BPNLS(SV40)-3xFLAG-P2A-EGFP
    

    Use

    CRISPR

    Tags

    BPNLS

    Expression

    Mammalian

    Mutation

    VRQR + A1285K mutations in SpCas9(D1135V/G1218R/A…

    Promoter

    CMV and T7

    Available Since

    Oct. 9, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCMV-SpCas9-VRQR(+L1245V)-P2A-EGFP (LRF49)

    Plasmid

    #242675

    Purpose

    CMV promoter expression plasmid for human codon optimized SpCas9-VRQR(L1245V)-BPNLS(SV40)-3xFLAG-P2A-EGFP

    Depositor

    Benjamin Kleinstiver

    Insert

    pCMV-SpCas9-VRQR(L1245V)-BPNLS(SV40)-3xFLAG-P2A-EGFP
    

    Use

    CRISPR

    Tags

    BPNLS

    Expression

    Mammalian

    Mutation

    VRQR + L1245V mutations in SpCas9(D1135V/G1218R/L…

    Promoter

    CMV and T7

    Available Since

    Oct. 9, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pCMV-SpCas9-VRQR(+D1332R)-P2A-EGFP (LRF45)

    Plasmid

    #242681

    Purpose

    CMV promoter expression plasmid for human codon optimized SpCas9-VRQR(D1332R)-BPNLS(SV40)-3xFLAG-P2A-EGFP

    Depositor

    Benjamin Kleinstiver

    Insert

    pCMV-SpCas9-VRQR(D1332R)-BPNLS(SV40)-3xFLAG-P2A-EGFP
    

    Use

    CRISPR

    Tags

    BPNLS

    Expression

    Mammalian

    Mutation

    VRQR + D1332R mutations in SpCas9(D1135V/G1218R/D…

    Promoter

    CMV and T7

    Available Since

    Oct. 9, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pCMV-SpCas9-VRQR(+L1111H)-P2A-EGFP (LRF269)

    Plasmid

    #242672

    Purpose

    CMV promoter expression plasmid for human codon optimized SpCas9-VRQR(L1111H)-BPNLS(SV40)-3xFLAG-P2A-EGFP

    Depositor

    Benjamin Kleinstiver

    Insert

    pCMV-SpCas9-VRQR(L1111H)-BPNLS(SV40)-3xFLAG-P2A-EGFP
    

    Use

    CRISPR

    Tags

    BPNLS

    Expression

    Mammalian

    Mutation

    VRQR + L1111H mutations in SpCas9(L1111H/D1135V/G…

    Promoter

    CMV and T7

    Available Since

    Oct. 9, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCMV-SpCas9-VRQR(+T1138K)-P2A-EGFP (LRF243)

    Plasmid

    #242674

    Purpose

    CMV promoter expression plasmid for human codon optimized SpCas9-VRQR(T1138K)-BPNLS(SV40)-3xFLAG-P2A-EGFP

    Depositor

    Benjamin Kleinstiver

    Insert

    pCMV-SpCas9-VRQR(T1138K)-BPNLS(SV40)-3xFLAG-P2A-EGFP
    

    Use

    CRISPR

    Tags

    BPNLS

    Expression

    Mammalian

    Mutation

    VRQR + T1138K mutations in SpCas9(D1135V/T1138K/G…

    Promoter

    CMV and T7

    Available Since

    Oct. 9, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pCMV-SpCas9-VRQR(+D1332K)-P2A-EGFP (KAC966)

    Plasmid

    #242680

    Purpose

    CMV promoter expression plasmid for human codon optimized SpCas9-VRQR(D1332K)-BPNLS(SV40)-3xFLAG-P2A-EGFP

    Depositor

    Benjamin Kleinstiver

    Insert

    pCMV-SpCas9-VRQR(D1332K)-BPNLS(SV40)-3xFLAG-P2A-EGFP
    

    Use

    CRISPR

    Tags

    BPNLS

    Expression

    Mammalian

    Mutation

    VRQR + D1332K mutations in SpCas9(D1135V/G1218R/D…

    Promoter

    CMV and T7

    Available Since

    Oct. 9, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. TRE3G-ZIM3-Cas9-P2A-GFP-PGK-Blasti

    Plasmid

    #239604

    Purpose

    Doxycycline inducible CRISPRgenee construct with a blasticidin resistance

    Depositor

    Philipp Rathert

    Article

    Stadager et al Cell Rep Methods. 2025 Jun 16;5(6):10107

    Insert

    ZIM3 KRAB domain (ZIM3 )
    

    Use

    CRISPR and Lentiviral

    Promoter

    TRE3G

    Available Since

    Sept. 30, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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18. EF1a-ZIM3-Cas9-P2A-GFP-PGK-Blasti

    Plasmid

    #239610

    Purpose

    Constitutive active CRISPRgenee construct with a blasticidin resistance

    Depositor

    Philipp Rathert

    Article

    Stadager et al Cell Rep Methods. 2025 Jun 16;5(6):10107

    Insert

    ZIM3 KRAB domain (ZIM3 )
    

    Use

    CRISPR and Lentiviral

    Available Since

    Sept. 30, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. nAPEX2-V5-zdCas9n-3x-FLAG-GSG-linker-P2A-mNeongreen

    Plasmid

    #241191

    Purpose

    Middle entry vector for mini-Golden subcloning platform; 3x-FLAG; mNeongreen

    Depositor

    Junsu Kang

    Insert

    3x-FLAG; mNeongreen
    

    Use

    Mini-golden

    Mutation

    TCGC linker

    Available Since

    Aug. 5, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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20. 3x-FLAG-nzdCas9-V5-APEX2n-GSG-linker-P2A-mNeongreen

    Plasmid

    #241190

    Purpose

    Middle entry vector for mini-Golden subcloning platform; V5-APEX2n; mNeongreen

    Depositor

    Junsu Kang

    Insert

    V5-APEX2n; mNeongreen
    

    Use

    Mini-golden

    Mutation

    TCGC linker

    Available Since

    Aug. 5, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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