164,296 results

1. pMP71-HLA-A0201-His

   Plasmid

   #108214

   Purpose

   HLA-A0201 his tagged

   Depositor

   Sébastien Walchli

   Article

   Lampen et al Immunohorizons. 2018 Jul 2;2(6):172-184.

   Insert

   major histocompatibility complex, class I, B (HLA-A Human)
   

   Use

   Retroviral

   Tags

   his

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 28, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. Bxb1-GT_AIO-TetOn_Down-Tandem Donor

   Plasmid

   #229783

   Purpose

   Bxb1-GT donor plasmid with downstream tandem syntax for all-in-one doxycycline-inducible expression (empty)

   Depositor

   Richard Davis

   Article

   Blanch-Asensio et al bioRxiv [Preprint]. 2024 Oct 17:2024.10.

   Insert

   LacZ; rtTA-T2A-mTagBFP2
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   TRE and CAG

   Available since

   Feb. 20, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. His6-TEV-CRBN-midi

   Plasmid

   #215330

   Purpose

   CRBN midi construct for use in crystallography and biophysical studies enabling ligand and degrader design

   Depositor

   Alessio Ciulli

   Article

   Kroupova et al Nat Commun. 2024 Oct 15;15(1):8885. doi:

   Insert

   CRBN (CRBN Human)
   

   Use

   Tags

   6xHis and TEV

   Expression

   Bacterial

   Mutation

   contains amino acids 41-187 followed by a Gly-Ser…

   Promoter

   lacI

   Available since

   March 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pENN226_CRISPRcharm

   Plasmid

   #220834

   Purpose

   CRISPRcharm expression in mammalian cells

   Depositor

   Jonathan Weissman

   Article

   Neumann et al Science. 2024 Jun 28;384(6703):ado7082.

   Insert

   CRISPRcharm
   

   Use

   CRISPR

   Tags

   P2A-TagBFP

   Expression

   Mammalian

   Mutation

   N/A

   Promoter

   CAG

   Available since

   June 25, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. EGFP-E-Syt2

   Plasmid

   #66831

   Purpose

   Mammalian Expression, Bacterial Expression

   Depositor

   Pietro De Camilli

   Article

   Giordano et al Cell. 2013 Jun 20;153(7):1494-509. doi:

   Insert

   E-Syt2 (ESYT2 Human)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 22, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLPCX-PECAMTS

   Plasmid

   #45850

   Depositor

   Martin Schwartz

   Article

   Conway et al Curr Biol. 2013 May 14. pii: S0960-9822(

   Insert

   PECAM1 (PECAM1 Human)
   

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   June 5, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAAV-CAG-GFP (AAV8)

   Viral Prep

   #37825-AAV8

   Purpose

   Ready-to-use AAV8 particles produced from pAAV-CAG-GFP (#37825). In addition to the viral particles, you will also receive purified pAAV-CAG-GFP plasmid DNA. CAG-driven GFP expression. These AAV preparations are suitable purity for injection into animals.

   Depositor

   Edward Boyden

   Promoter

   CAG

   Tags

   GFP

   Available since

   May 28, 2019

   Availability

   Academic Institutions and Nonprofits only

8. pAAV-hSyn Con/Fon EYFP (AAV Retrograde)

   Viral Prep

   #55650-AAVrg

   Purpose

   Ready-to-use AAV Retrograde particles produced from pAAV-hSyn Con/Fon EYFP (#55650). In addition to the viral particles, you will also receive purified pAAV-hSyn Con/Fon EYFP plasmid DNA. Synapsin driven, Cre and Flp-dependent EYFP expression. These AAV were produced with a retrograde serotype, which permits retrograde access to projection neurons. These AAV preparations are suitable purity for injection into animals.

   Depositor

   Karl Deisseroth

   Article

   Fenno et al Nat Methods. 2014 Jul;11(7):763-72. doi:

   Promoter

   Syn

   Tags

   EYFP (Cre- and Flp-dependent)

   Available since

   Sept. 8, 2020

   Availability

   Academic Institutions and Nonprofits only

9. pMAL-7xHis-TEV-hexa

   Plasmid

   #222868

   Purpose

   Expresses His-tagged mutant (C19V/L56V/C110V/C130S/S135G/S219V) TEV protease in E. coli

   Depositor

   Nikolaus Loening

   Article

   Davis et al Protein Sci. 2025 Mar;34(3):e70049. doi:

   Insert

   TEV protease
   

   Use

   Tags

   7xHis and MBP

   Expression

   Bacterial

   Mutation

   C19V, L56V, C110V, C130S, S135G, S219V. Insert in…

   Promoter

   Available since

   Feb. 19, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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10. pDEST-SorLA-v1

    Plasmid

    #154892

    Purpose

    Expresses SorLA fused to Venus fragment 1 (v1) for use in bimolecular fluorescence complementation (BiFC) assays

    Depositor

    Johanna Ivaska

    Article

    Al-Akhrass et al Oncogene. 2021 Feb;40(7):1300-1317. doi:

    Insert

    SORL1 (SORL1 Human)
    

    Use

    Tags

    Venus fragment 1 (v1)

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 27, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAAV-hsyn-GRAB_rDA1h (AAV9)

    Viral Prep

    #140557-AAV9

    Purpose

    Ready-to-use AAV9 particles produced from pAAV-hsyn-GRAB_rDA1h (#140557). In addition to the viral particles, you will also receive purified pAAV-hsyn-GRAB_rDA1h plasmid DNA. Synapsin-driven expression of red fluorescent, high affinity dopamine sensor GRAB_rDA1h. These AAV preparations are suitable purity for injection into animals.

    Depositor

    Yulong Li

    Article

    Sun et al Nat Methods. 2020 Nov;17(11):1156-1166.

    Promoter

    Syn

    Tags

    None

    Available since

    Aug. 31, 2021

    Availability

    Academic Institutions and Nonprofits only

12. pmScarlet3_C1

    Plasmid

    #189753

    Purpose

    C1 Cloning vector for mammalian cells incorporating mScarlet3 red fluorescent protein

    Depositor

    Dorus Gadella

    Article

    Gadella TWJ et al Nat Methods. 2023 Apr;20(4):541-545. doi

    Insert

    mScarlet3
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pAAV-EFS-DIO-GRAB_Ado1.0

    Plasmid

    #153291

    Purpose

    Express the adenosine sensor GRAB_Ado1.0 in Cre positive cells

    Depositor

    Yulong Li

    Article

    Peng et al Science. 2020 Sep 4;369(6508). pii: 369/

    Insert

    Adenosine sensor GRAB_Ado1.0
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    EFS

    Available since

    July 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pLKO.3G_X7

    Plasmid

    #171213

    Purpose

    3rd gen lentiviral backbone for cloning and expression of new shRNA sequences. Uses eGFP for selection. Please see cloning information for suggested restriction sites

    Depositor

    Jenny Wang

    Insert

    N/A; empty backbone
    

    Use

    Lentiviral and RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 22, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. 2XMyc-LRRK2-WT

    Plasmid

    #25361

    Depositor

    Mark Cookson

    Article

    J Biol Chem. 2008 Jun 13;283(24):16906-1

    Insert

    LRRK2 (LRRK2 Human)
    

    Use

    Tags

    2XMyc

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 17, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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16. pEGFP-N1 human cofilin WT

    Plasmid

    #50859

    Purpose

    expresses human cofilin fused to EGFP in mammalian cells

    Depositor

    James Bamburg

    Article

    Garvalov et al J Neurosci. 2007 Nov 28;27(48):13117-29.

    Insert

    cofilin 1 (CFL1 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 5, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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17. pET29a-YS14

    Plasmid

    #66890

    Purpose

    Polycistronic coexpression of Xenopus laevis histones (H2A, H2B, H3 and H4)

    Depositor

    Jung-Hyun Min

    Article

    Shim et al Anal Biochem. 2012 Aug 15;427(2):190-2.

    Inserts

    His6-Thrombin-Xenopus laevis histones H2A
    Xenopus laevis histones H2B
    Xenopus laevis histones H3
    Xenopus laevis histones H4-Thrombin
    

    Use

    Tags

    H2A, H2B, H3, H4, His6 tag, and Thrombin

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Sept. 8, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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18. pEff

    Plasmid

    #97423

    Purpose

    Transient expression of recombinant proteins in plants. The plasmid contains gfp, which is replaced by the gene of interest.

    Depositor

    Nikolai Ravin

    Article

    Mardanova et al Front Plant Sci. 2017 Feb 28;8:247. doi:

    Type

    Empty backbone

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    July 31, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. pLenti6/V5-p53_wt p53

    Plasmid

    #22945

    Purpose

    3rd generation lentiviral vector encoding human p53 with a C-terminal V5 tag

    Depositor

    Bernard Futscher

    Article

    Junk et al Neoplasia. 2008 May . 10(5):450-61.

    Insert

    tumor protein 53 (TP53 Human)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 27, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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20. mCherry-NbALFA

    Plasmid

    #159987

    Purpose

    For the expresson and purification of the mCherry-tagged anti-ALFA tag nanobody (NbALFA)

    Depositor

    Motoyuki Hattori

    Article

    Jin et al Commun Biol. 2021 Mar 19;4(1):366. doi:

    Insert

    mCherry-NbALFA
    

    Use

    Tags

    8histidine-HRV3C protease cleavage site

    Expression

    Bacterial

    Mutation

    3GS linker between mCherry and NbALFA

    Promoter

    Available since

    Oct. 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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