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M38 TOP-dGFP

Plasmid

#17114

Depositor

Randall Moon

Insert

Tcf/Lef sites (Ctnnb1 )

Tags

GFP

Available since

Feb. 1, 2008

Availability

Academic Institutions and Nonprofits only

Cited by

8 Articles

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pcDNA3-TLR3-CFP

Plasmid

#13641

Depositor

Doug Golenbock

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Toll-Like Receptor 3 (TLR3 Human)

Tags

CFP

Expression

Mammalian

Available since

June 12, 2007

Availability

Academic Institutions and Nonprofits only

Cited by

7 Articles

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pcDNA3-TLR3-CFP

Plasmid

#13641

Depositor

Doug Golenbock

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Toll-Like Receptor 3 (TLR3 Human)

Tags

CFP

Expression

Mammalian

Available since

June 12, 2007

Availability

Academic Institutions and Nonprofits only

Cited by

7 Articles

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GFP-rab7 DN

Plasmid

#12660

Depositor

Richard Pagano

Publication

Choudhury et al J Clin Invest. 2002 Jun . 109(12):1541-5

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

RAB7 (Homo sapiens) (RAB7A Human)

Tags

GFP and HA

Expression

Mammalian

Mutation

T22N

Available since

Oct. 16, 2006

Availability

Academic Institutions and Nonprofits only

Cited by

35 Articles

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GFP-rab7 DN

Plasmid

#12660

Depositor

Richard Pagano

Publication

Choudhury et al J Clin Invest. 2002 Jun . 109(12):1541-5

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

RAB7 (Homo sapiens) (RAB7A Human)

Tags

GFP and HA

Expression

Mammalian

Mutation

T22N

Available since

Oct. 16, 2006

Availability

Academic Institutions and Nonprofits only

Cited by

35 Articles

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HA-FOXO3a TM

Plasmid

#1788

Depositor

Michael Greenberg

Publication

Brunet et al Cell 1999 Mar 19;96(6):857-68.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

FOXO3a (FOXO3 Human)

Tags

HA (YDVPDYASL)

Expression

Mammalian

Mutation

T32A, S253A, S315A (no longer detectably phosphor…

Available since

April 20, 2006

Availability

Academic Institutions and Nonprofits only

Cited by

12 Articles

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HA-FOXO3a TM

Plasmid

#1788

Depositor

Michael Greenberg

Publication

Brunet et al Cell 1999 Mar 19;96(6):857-68.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

FOXO3a (FOXO3 Human)

Tags

HA (YDVPDYASL)

Expression

Mammalian

Mutation

T32A, S253A, S315A (no longer detectably phosphor…

Available since

April 20, 2006

Availability

Academic Institutions and Nonprofits only

Cited by

12 Articles

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pHR-UCOE-SFFV-dCas9-mCherry-ZIM3-KRAB

Plasmid

#154473

Purpose

Expresses dCas9 fused to mCherry and the KRAB domain of ZIM3

Depositor

Mikko Taipale

Publication

Alerasool et al Nat Methods. 2020 Nov;17(11):1093-1096.

Insert

ZIM3 (ZIM3 Human)

Use

Lentiviral

Tags

HA

Expression

Mammalian

Mutation

Includes ZIM3 aa 1-100

Promoter

SFFV

Available since

Oct. 5, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

10 Articles

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pHR-UCOE-SFFV-dCas9-mCherry-ZIM3-KRAB

Plasmid

#154473

Purpose

Expresses dCas9 fused to mCherry and the KRAB domain of ZIM3

Depositor

Mikko Taipale

Publication

Alerasool et al Nat Methods. 2020 Nov;17(11):1093-1096.

Insert

ZIM3 (ZIM3 Human)

Use

Lentiviral

Tags

HA

Expression

Mammalian

Mutation

Includes ZIM3 aa 1-100

Promoter

SFFV

Available since

Oct. 5, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

10 Articles

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pAAV-CAG-GFP (AAV1)

Viral prep

#37825-AAV1

Purpose

Ready-to-use AAV1 particles produced from pAAV-CAG-GFP (#37825). In addition to the viral particles, you will also receive purified pAAV-CAG-GFP plasmid DNA. CAG-driven GFP expression. These AAV preparations are suitable purity for injection into animals.

Depositor

Edward Boyden

Promoter

CAG

Tags

GFP

Available since

May 10, 2019

Availability

Academic Institutions and Nonprofits only
Human CRISPRi sgRNA library Dolcetto in backbone XPR_050

Pooled library

#1000000114

Purpose

Genome-wide CRISPR gRNA pooled library for inhibition of human genes

Depositor

John Doench, David Root

Available since

July 13, 2018

Availability

Academic Institutions and Nonprofits only
pAAV.Syn.Flex.GCaMP6f.WPRE.SV40 (AAV5)

Viral prep

#100833-AAV5

Purpose

Ready-to-use AAV5 particles produced from pAAV.Syn.Flex.GCaMP6f.WPRE.SV40 (#100833). In addition to the viral particles, you will also receive purified pAAV.Syn.Flex.GCaMP6f.WPRE.SV40 plasmid DNA. Syn-driven, Cre-dependent GCaMP6f calcium sensor. These AAV preparations are suitable purity for injection into animals.

Depositor

Douglas Kim, GENIE Project

Publication

Chen et al Nature. 2013 Jul 18;499(7458):295-300. d

Promoter

Syn

Available since

May 11, 2018

Availability

Academic Institutions and Nonprofits only
pAAVS1-NDi-CRISPRi (Gen2)

Plasmid

#73498

Purpose

Dox-inducible CRISPR interference (CRISPRi) knock in construct into the AAVS1 locus

Depositor

Bruce Conklin

Publication

Mandegar et al Cell Stem Cell. 2016 Apr 7;18(4):541-53.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Inserts

dCas9-KRAB
rtTA

Use

CRISPR

Tags

HA, KRAB, and NLS

Expression

Mammalian

Mutation

D10A, H840A (catalytically deactivated Cas9)

Promoter

CAG and TRE3G

Available since

Dec. 7, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

14 Articles

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pAAVS1-NDi-CRISPRi (Gen2)

Plasmid

#73498

Purpose

Dox-inducible CRISPR interference (CRISPRi) knock in construct into the AAVS1 locus

Depositor

Bruce Conklin

Publication

Mandegar et al Cell Stem Cell. 2016 Apr 7;18(4):541-53.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Inserts

dCas9-KRAB
rtTA

Use

CRISPR

Tags

HA, KRAB, and NLS

Expression

Mammalian

Mutation

D10A, H840A (catalytically deactivated Cas9)

Promoter

CAG and TRE3G

Available since

Dec. 7, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

14 Articles

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pACYC-GroEL/ES-TF

Plasmid

#83923

Purpose

Co-expresses cytoplasmic copies of the Gro EL/ES and Trigger Factor protein folding chaperones.

Depositor

Karl Griswold

Publication

Lamppa et al J Biotechnol. 2013 Mar 10;164(1):1-8. do

Inserts

Gro EL/ES
Trigger Factor

Expression

Bacterial

Promoter

T7

Available since

Nov. 28, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pACYC-GroEL/ES-TF

Plasmid

#83923

Purpose

Co-expresses cytoplasmic copies of the Gro EL/ES and Trigger Factor protein folding chaperones.

Depositor

Karl Griswold

Publication

Lamppa et al J Biotechnol. 2013 Mar 10;164(1):1-8. do

Inserts

Gro EL/ES
Trigger Factor

Expression

Bacterial

Promoter

T7

Available since

Nov. 28, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pdCas9::BFP-humanized

Plasmid

#44247

Purpose

Expression of a catalytically inactive, human codon-optimized Cas9-BFP fusion under the control of Murine Stem Cell retroVirus LTR promoter for mammalian gene knockdown

Depositor

Stanley Qi

Publication

Qi et al Cell. 2013 Feb 28;152(5):1173-83. doi: 1

Insert

dCas9 fused to BFP

Use

CRISPR and Retroviral

Tags

2xNLS & tagBFP

Expression

Mammalian

Mutation

D10A H840A (catalytically inactive)

Promoter

MSCV LTR promoter

Available since

April 8, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

6 Articles

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pdCas9::BFP-humanized

Plasmid

#44247

Purpose

Expression of a catalytically inactive, human codon-optimized Cas9-BFP fusion under the control of Murine Stem Cell retroVirus LTR promoter for mammalian gene knockdown

Depositor

Stanley Qi

Publication

Qi et al Cell. 2013 Feb 28;152(5):1173-83. doi: 1

Insert

dCas9 fused to BFP

Use

CRISPR and Retroviral

Tags

2xNLS & tagBFP

Expression

Mammalian

Mutation

D10A H840A (catalytically inactive)

Promoter

MSCV LTR promoter

Available since

April 8, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

6 Articles

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PL-SIN-Nanog-EGFP

Plasmid

#21321

Purpose

Lentiviral vector, expresses EGFP under Nanog promoter

Depositor

James Ellis

Publication

Hotta et al Nat Methods. 2009 May . 6(5):370-6.

Insert

Enhanced Green Fluorescence Protein

Use

Lentiviral

Expression

Mammalian

Promoter

Nanog promoter region

Available since

June 1, 2009

Availability

Academic Institutions and Nonprofits only

Cited by

7 Articles

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PL-SIN-Nanog-EGFP

Plasmid

#21321

Purpose

Lentiviral vector, expresses EGFP under Nanog promoter

Depositor

James Ellis

Publication

Hotta et al Nat Methods. 2009 May . 6(5):370-6.

Insert

Enhanced Green Fluorescence Protein

Use

Lentiviral

Expression

Mammalian

Promoter

Nanog promoter region

Available since

June 1, 2009

Availability

Academic Institutions and Nonprofits only

Cited by

7 Articles

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