167,564 results

1. pITR-AAV-Rep2Cap9-GFP

   Plasmid

   #199744

   Purpose

   Expresses AAV2 Rep and AAV9 Cap, with GFP replacing a region of Cap, within AAV2 ITRs.

   Depositor

   Aravind Asokan

   Article

   Gonzalez et al Nat Protoc. 2023 Sep 21. doi: 10.1038/s4

   Insert

   AAV9 Cap w/ GFP insert
   

   Use

   AAV

   Expression

   Mammalian

   Mutation

   Replaced amino acids 356-736 in Cap with GFP

   Available Since

   Oct. 31, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCMV-SUN1 mScarlet

   Plasmid

   #187683

   Purpose

   In vivo visualization of SUN1

   Depositor

   Mary Beckerle

   Insert

   SUN1 (Sun1 Mouse)
   

   Tags

   mScarlet

   Expression

   Mammalian

   Mutation

   WT

   Promoter

   CMV

   Available Since

   Sept. 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. FP5369 KIF17MD-flag- FIREmate

   Plasmid

   #216724

   Purpose

   Expresses motor domain of KIF17 fused to FIREmate in mamallian cells

   Depositor

   Franck Perez

   Article

   Bottone et al Nat Methods. 2023 Aug 28. doi: 10.1038/s

   Insert

   KIF17 (KIF17 Human)
   

   Tags

   FIREmate and Flag

   Expression

   Mammalian

   Mutation

   1-547

   Promoter

   CMV

   Available Since

   July 10, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. mApple-TNFa-RUSH

   Plasmid

   #166902

   Purpose

   RUSH cargo: Str-KDEL_IRES_Tumor Necrosis Factor alpha (TNFa)-SBP-mApple

   Depositor

   Jennifer Lippincott-Schwartz

   Article

   Weigel et al Cell. 2021 Apr 29;184(9):2412-2429.e16.

   Insert

   mApple
   

   Expression

   Mammalian

   Available Since

   April 14, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. lentiSAM v2 (Puro)

   Plasmid

   #92062

   Purpose

   Modified version of lentiSAM v2, a lenti sgRNA cloning backbone with MS2 loops at tetraloop/stemloop 2, dCas9-VP64, and puro resistance marker. Contains BsmBI sites for insertion of spacer sequences.

   Depositor

   Adam Karpf

   Article

   Barger et al Elife. 2021 Apr 23;10. pii: 55070. doi:

   Type

   Empty backbone

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   U6 (sgRNA) and EF1a (dCas9-VP64)

   Available Since

   May 31, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAAV-SCP1-dSa-VPR mini.

   Plasmid

   #99685

   Purpose

   Expresses dSa Cas9 fused to optimized combination of truncated activation domains from SCP1 promoter

   Depositor

   George Church

   Article

   Vora et al bioRxiv 2018. 298620

   Insert

   dCas9
   

   Use

   AAV

   Tags

   VPR mini

   Mutation

   dead Cas9

   Available Since

   Sept. 18, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pWPI-GFP-3xFLAG

   Plasmid

   #201639

   Purpose

   Over-expression of 3xFLAG-tagged GFP

   Depositor

   Alexis Jourdain, Vamsi Mootha

   Article

   Skinner et al Nat Metab. 2023 May;5(5):765-776. doi: 1

   Insert

   GFP
   

   Use

   Lentiviral

   Tags

   3xFLAG

   Available Since

   June 22, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. TFORF2546

   Plasmid

   #144209

   Purpose

   Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

   Depositor

   Feng Zhang

   Article

   Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

   Insert

   CEBPA (CEBPA Human)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   EF1a

   Available Since

   Aug. 14, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pDae029 (eMutaT7[TadA-8e])

   Plasmid

   #187620

   Purpose

   Expresses eMutaT7[TadA-8e] for mutagenizing genes downstream from a T7 promoter in e.coli

   Depositor

   Seokhee Kim

   Article

   Seo et al Nucleic Acids Res. 2023 Apr 18:gkad266.

   Insert

   TadA-8e-T7RNAP
   

   Expression

   Bacterial

   Available Since

   Aug. 2, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. dRED

    Plasmid

    #139836

    Purpose

    Expresses dRNase H1-EGFP-dCas9 fusion protein in human cells

    Depositor

    Karim Mekhail

    Article

    Abraham et al Nature. 2020 Sep;585(7824):298-302. doi:

    Insert

    dRNase H1-EGFP-dCas9 (RNASEH1 dRNase H1 (H. sapiens), dCas9 (S. pyogenes))
    

    Expression

    Mammalian

    Mutation

    RNase H1 protein contains D210N mutation. dCas9 …

    Promoter

    tet-inducible CMV

    Available Since

    Sept. 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pSIL-eGFP

    Plasmid

    #52675

    Purpose

    shRNA silencing plasmid with eGFP transfection marker

    Depositor

    Johannes Hell

    Article

    Schnizler et al J Biol Chem. 2009 Jan 30;284(5):2697-705

    Type

    Empty backbone

    Use

    RNAi

    Tags

    CMV-EGFP

    Promoter

    CMV and U6

    Available Since

    June 10, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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12. pSN001 pME minprom_mCerulean

    Plasmid

    #195959

    Purpose

    Gateway pME fluorophore

    Depositor

    Alexa Burger, Christian Mosimann

    Article

    Kemmler et al Development. 2023 Mar 28:dev.201531. doi

    Insert

    mouse beta-globin minimal promoter mCerulean
    

    Use

    Other

    Available Since

    Feb. 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pTRIP-SFFV-mtagBFP-2A STING V155M

    Plasmid

    #102591

    Purpose

    Lentivector to express STING V155M and mTagBFP

    Depositor

    Nicolas Manel

    Article

    Cerboni et al J Exp Med. 2017 Jun 5;214(6):1769-1785.

    Insert

    STING (STING1 Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Mutation

    V155M

    Promoter

    SFFV

    Available Since

    Jan. 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. pKD3

    Plasmid

    #45604

    Depositor

    Barry L. Wanner

    Article

    Datsenko et al Proc Natl Acad Sci U S A. 2000 Jun 6;97(

    Insert

    FRT-cat-FRT
    

    Expression

    Bacterial

    Promoter

    R6K

    Available Since

    June 10, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCDNA3.1_3XFlag

    Plasmid

    #182494

    Purpose

    Empty plasmid containing Flag tag for C-terminal tagging

    Depositor

    Paolo Comoglio

    Article

    Cerqua et al Life Sci Alliance. 2022 May 30;5(10). pi

    Insert

    3xFlag with 5 Glycolic spacer
    

    Available Since

    June 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCfB2197

    Plasmid

    #67551

    Purpose

    EasyClone system-based yeast integrative vector carrying loxP-flanked natMX marker, integration into S. cerevisiae XII-1 chromosomal location, USER site for cloning, amp resistance

    Depositor

    Irina Borodina

    Article

    Stovicek et al J Ind Microbiol Biotechnol. 2015 Sep 16.

    Type

    Empty backbone

    Use

    Cre/Lox; Integrative

    Expression

    Yeast

    Available Since

    March 10, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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17. pSNAP-CaaX Control Plasmid

    Plasmid

    #101133

    Purpose

    Expression of SNAP-Tag and CaaX box (Farnesylation Signal) in Mammalian cells

    Depositor

    New England Biolabs, Ana Egana

    Insert

    CaaX Box
    

    Tags

    SNAP-tag (SNAP26m)

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 27, 2017

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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18. pRK5-FLAG-C7orf59

    Plasmid

    #42332

    Depositor

    David Sabatini

    Article

    Bar-Peled et al Cell. 2012 Sep 14;150(6):1196-208. doi:

    Insert

    C7orf59 (LAMTOR4 Human)
    

    Tags

    Flag

    Expression

    Mammalian

    Promoter

    CMV, SP6

    Available Since

    March 6, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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19. pAAV-CAG-Electra2-P2A-EGFP

    Plasmid

    #184936

    Purpose

    Expresses blue fluorescent protein Electra2 and EGFP in mammalian cells

    Depositor

    Kiryl Piatkevich

    Article

    Papadaki et al Sci Rep. 2022 Jun 17;12(1):10190. doi: 1

    Insert

    Electra2-P2A-EGFP
    

    Use

    AAV

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    Sept. 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. pACEBac1-PSMA1-PSMA2-PSMA3-PSMA4-PSMA5-PSMA6-PSMA7

    Plasmid

    #214138

    Purpose

    Insect cell expression of human 20S CP alpha-subunits

    Depositor

    Brenda Schulman

    Article

    Adolf et al Nat Struct Mol Biol. 2024 Apr 10. doi: 1

    Inserts

    PSMA1 (PSMA1 Human)
    PSMA2 (PSMA2 Human)
    PSMA3 (PSMA3 Human)
    PSMA4 (PSMA4 Human)
    PSMA5 (PSMA5 Human)
    PSMA6 (PSMA6 Human)
    PSMA7 (PSMA7 Human)
    

    Expression

    Insect

    Promoter

    polyhedrin

    Available Since

    May 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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