We narrowed to 3,262 results for: GAI

1. Lenti-sgErich3#2/Cre

   Plasmid

   #193212

   Purpose

   Lentiviral vector expressing Cre recombinase (driven by mPGK promoter) and an sgRNA against the mouse Erich3 gene

   Depositor

   Julien Sage

   Article

   Lee et al Cell Rep. 2023 Jan 13;42(1):111990. doi:

   Insert

   sgErich3#2 (4922501L14Rik Mouse)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Dec. 12, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCAG-eSpCas9-2A-GFP-sgRNA-RDH11

   Plasmid

   #161924

   Purpose

   To generate RDH11 KO in human cells. Co-expresses eSpCas9(1.1), GFP and a guide RNA against RDH11 exon 2.

   Depositor

   Sébastien Britton

   Article

   Demange et al Elife. 2022 May 10;11. pii: 73913. doi:

   Insert

   sgRNA targeting RDH11 exon 2
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   May 12, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pKHH030_sgAMBRA1#2

   Plasmid

   #174147

   Purpose

   Lentiviral vector expressing a sgRNA against the human AMBRA1 gene

   Depositor

   Julien Sage

   Article

   Chaikovsky et al Nature. 2021 Apr;592(7856):794-798. doi:

   Insert

   AMBRA (AMBRA1 Human)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hU6

   Available since

   Sept. 13, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pKHH030_sgAMBRA1#1

   Plasmid

   #174146

   Purpose

   Lentiviral vector expressing a sgRNA against the human AMBRA1 gene

   Depositor

   Julien Sage

   Article

   Chaikovsky et al Nature. 2021 Apr;592(7856):794-798. doi:

   Insert

   AMBRA (AMBRA1 Human)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hU6

   Available since

   Sept. 13, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pGMC00001

   Plasmid

   #166718

   Purpose

   sgRNA against human BAP1

   Depositor

   Raj Chari

   Article

   Rathkey et al J Thorac Oncol. 2020 May;15(5):843-859.

   Insert

   BAP1 (BAP1 Human)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pGMC00002

   Plasmid

   #166719

   Purpose

   sgRNA against human BAP1

   Depositor

   Raj Chari

   Article

   Rathkey et al J Thorac Oncol. 2020 May;15(5):843-859.

   Insert

   BAP1 (BAP1 Human)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pMY-CD90.2-P2A-BlastDYK-Rluc_miR

   Plasmid

   #163341

   Purpose

   Retroviral vector for negative control of knockdown expressing a non-target miR against Renilla luciferase and expression of a Ly6G surface marker and a DYK-tagged Blasticidin selection marker

   Depositor

   Teunis Geijtenbeek, Louis Ates

   Article

   van der Donk et al Biol Open. 2022 Feb 15;11(2):bio059032.

   Insert

   CD90.2 (Thy1 Mouse)
   

   Use

   RNAi and Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   LTR

   Available since

   Jan. 27, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pMX-CAG-CD90.2-Rluc_miR

   Plasmid

   #163331

   Purpose

   Retroviral vector for negative control of knockdown expressing a non-target miR against Renilla luciferase and expression of a CD90.2 surface marker under control of the CAG promoter.

   Depositor

   Teunis Geijtenbeek, Louis Ates

   Article

   van der Donk et al Biol Open. 2022 Feb 15;11(2):bio059032.

   Insert

   CD90.2 (Thy1 Mouse)
   

   Use

   RNAi and Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   Jan. 27, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pMX-hFTH1-CD90.2-Rluc_miR

   Plasmid

   #163330

   Purpose

   Retroviral vector for negative control of knockdown expressing a non-target miR against Renilla luciferase and expression of a CD90.2 surface marker under control of the human FTH1 promoter.

   Depositor

   Teunis Geijtenbeek, Louis Ates

   Article

   van der Donk et al Biol Open. 2022 Feb 15;11(2):bio059032.

   Insert

   CD90.2 (Thy1 Mouse)
   

   Use

   RNAi and Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hFTH1

   Available since

   Jan. 27, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pMX-hPGK-CD90.2-Rluc_miR

    Plasmid

    #163329

    Purpose

    Retroviral vector for negative control of knockdown expressing a non-target miR against Renilla luciferase and expression of a CD90.2 surface marker under control of the human PGK promoter.

    Depositor

    Teunis Geijtenbeek, Louis Ates

    Article

    van der Donk et al Biol Open. 2022 Feb 15;11(2):bio059032.

    Insert

    CD90.2 (Thy1 Mouse)
    

    Use

    RNAi and Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hPGK

    Available since

    Jan. 27, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. CXCR4-SZ158a-sfGFP

    Plasmid

    #162446

    Purpose

    Expression in HEK293T cell and compete ligand singaling against full-length receptors

    Depositor

    Shuguang Zhang

    Article

    Qing et al iScience. 2020 Oct 28;23(12):101670. doi

    Insert

    C-X-C chemokine receptor type 4 (CXCR4 Human)
    

    Use

    Tags

    sfGFP

    Expression

    Mammalian

    Mutation

    truncation from aa52 to aa257

    Promoter

    CMV

    Available since

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCDH-3_Flag-YTHDF2-RNA-MUT (nonsense mutation of sgYTHDF2-1)

    Plasmid

    #232948

    Purpose

    Expresses the RNA-binding-deficient mutant of YTHDF2 with synonymous mutations for resistance against sgYTHDF2-1-mediated knockdown

    Depositor

    Jianjun Chen

    Article

    Chen et al Cell. 2024 Dec 12:S0092-8674(24)01324-2.

    Insert

    YTHDF2-RNA-MUT (YTHDF2 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    RNA binding mutation (K416A+R527A), synonymous mu…

    Promoter

    Available since

    May 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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13. pDONR223-PCTK1

    Plasmid

    #23754

    Depositor

    David Root, William Hahn

    Article

    Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

    Insert

    PCTK1 (CDK16 Human)
    

    Use

    Gateway donor vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 30, 2010

    Availability

    Academic Institutions and Nonprofits only

14. pWZL Neo Myr Flag PCTK1

    Plasmid

    #20560

    Depositor

    Jean Zhao, William Hahn

    Article

    Boehm et al Cell. 2007 Jun 15. 129(6):1065-1079.

    Insert

    PCTK1 (CDK16 Human)
    

    Use

    Retroviral

    Tags

    Flag and Myr

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 27, 2009

    Availability

    Academic Institutions and Nonprofits only

15. pLV-RnSyt1-sgRNA2-dCas9-KRAB-TagBFP2 (identifier AAAA-0246)

    Plasmid

    #202555

    Purpose

    Synaptotagmin-1 sgRNA2 with TagBFP2

    Depositor

    Frederic Meunier

    Article

    Joensuu et al EMBO J. 2023 Jul 3;42(13):e112095. doi:

    Insert

    CRISPRi single guide RNA sequence against 5’-UTR of rat synaptotagmin-1 (Syt1) gene (insert 5’ - CACCAGTACTCGCGTGCCTCGCACCGG) (Syt1 Rat)
    

    Use

    CRISPR and Lentiviral

    Tags

    3xFlag (N-terminus of Cas9-KRAB), T2A-TagBFP2

    Expression

    Mutation

    Promoter

    Available since

    Dec. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pLV-RnSyt1-sgRNA3-dCas9-KRAB-TagBFP2 (Identifier AAAA-0247)

    Plasmid

    #202556

    Purpose

    Synaptotagmin-1 sgRNA3 with TagBFP2

    Depositor

    Frederic Meunier

    Article

    Joensuu et al EMBO J. 2023 Jul 3;42(13):e112095. doi:

    Insert

    CRISPRi single guide RNA sequence against 5’-UTR of rat synaptotagmin-1 (Syt1) gene (insert 5’ - CACCTCCTCCTGCAGCGGCAGCATCGG) (Syt1 Rat)
    

    Use

    CRISPR and Lentiviral

    Tags

    3xFlag (N-terminus of Cas9-KRAB), T2A-TagBFP2

    Expression

    Mutation

    Promoter

    Available since

    Dec. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pLV-RnSyt1-sgRNA1-dCas9-KRAB-TagBFP2 (identifier AAAA-0245)

    Plasmid

    #202554

    Purpose

    Synaptotagmin-1 sgRNA1 with TagBFP2

    Depositor

    Frederic Meunier

    Article

    Joensuu et al EMBO J. 2023 Jul 3;42(13):e112095. doi:

    Insert

    CRISPRi single guide RNA sequence against 5’-UTR of rat synaptotagmin-1 (Syt1) gene (insert 5’ -CACCGCGTGCCTCGCACCGGTCCGCGG) (Syt1 R. norvegius (gRNA))
    

    Use

    CRISPR and Lentiviral

    Tags

    3xFlag (N-terminus of Cas9-KRAB), T2A-TagBFP2

    Expression

    Mutation

    Promoter

    Available since

    Dec. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. BAP1-wt (siRNA resistant)

    Plasmid

    #108439

    Purpose

    Stable expression of BAP1-wt in mammalian cells, gene has an introduced siRNA-resistance against siBAP1.

    Depositor

    Emanuela Felley-Bosco, Rolf Stahel

    Article

    Okonska et al Mol Cancer Ther. 2019 Oct 16. pii: 1535-

    Insert

    BAP1 (BAP1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    c.1641C>T, c.1644T>A, c.1647T>C, c.1650C…

    Promoter

    CMV

    Available since

    Nov. 22, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. BAP1-C91A (siRNA resistant)

    Plasmid

    #108438

    Purpose

    Stable expression of BAP1-C91A (catalytically inactive mutant) in mammalian cells, gene has an introduced siRNA-resistance against siBAP1.

    Depositor

    Emanuela Felley-Bosco, Rolf Stahel

    Article

    Okonska et al Mol Cancer Ther. 2019 Oct 16. pii: 1535-

    Insert

    BAP1 (BAP1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    C91A: c.271T>G, c.272G>C; others: c.1641C&g…

    Promoter

    CMV

    Available since

    Nov. 22, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. lentiSAMv2 EGFP-guide1

    Plasmid

    #118166

    Purpose

    CRISPRa negative control. Catalytically inactive Cas9 from S. pyogenes and VP64 with 2A-BlastR, and sgRNA1 against the EGFP CDS

    Depositor

    Jorge Ferrer

    Article

    Miguel-Escalada et al Nat Genet. 2019 Jun 28. pii: 10.1038/s41

    Insert

    dCas9-VP64-2A-BlastR
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a core and U6

    Available since

    April 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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