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1183_pAAV-U6-Ex14-gRNAd-CB-EmGFP

Plasmid

#89061

Purpose

AAV-gRNA targeting the murine Ldlr gene

Depositor

William Lagor

Article

Jarrett et al Sci Rep. 2017 Mar 16;7:44624. doi: 10.10

Insert

EmGFP

Use

AAV and CRISPR

Expression

Mammalian

Promoter

Chicken beta actin with partial CMV enhancer

Available Since

April 11, 2017

Availability

Academic Institutions and Nonprofits only

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pX330-sgRNA_Dicer_3

Plasmid

#68809

Purpose

specific sgRNA against mouse Dicer1 gene cloned in the pX330 backbone (Addgene Number 42230). Generation of Dicer1 knockout mESCs.

Depositor

Constance Ciaudo

Article

Bodak et al FEBS Open Bio. 2017 Jan 11;7(2):204-220.

Insert

sgRNA mouse Dicer1

Use

CRISPR and Mouse Targeting

Expression

Bacterial and Mammalian

Available Since

Feb. 13, 2017

Availability

Academic Institutions and Nonprofits only

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pET28a-Hook3 aa 1-160-GCN4

Plasmid

#74608

Purpose

Expresses artificially dimerized Hook domain of Hook3 in bacteria for purification

Depositor

Ron Vale

Article

Schroeder et al J Cell Biol. 2016 Aug 1;214(3):309-18. d

Insert

human Hook3 amino acids 1-160 (HOOK3 Human)

Tags

6x His, GCN4, Strep II, and superfolder GFP

Expression

Bacterial

Available Since

Aug. 5, 2016

Availability

Academic Institutions and Nonprofits only

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Mouse NCKX3 (pcDNA3.1+)

Plasmid

#75204

Purpose

Mammalian expression of SLC24A3

Depositor

Jonathan Lytton

Article

Kraev et al J Biol Chem. 2001 Jun 22;276(25):23161-7

Insert

SLC24A3 (Slc24a3 Mouse)

Expression

Mammalian

Promoter

CMV

Available Since

June 21, 2016

Availability

Academic Institutions and Nonprofits only

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pTHsp18.2 (GB0035)

Plasmid

#68186

Purpose

Provides the Arabidopsis thaliana heat shock protein 18.2 terminator as a level 0 GoldenBraid part

Depositor

Diego Orzaez

Article

Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

Insert

AtHsp18.2 terminator

Use

Synthetic Biology

Mutation

BsaI and BsmBI sites removed

Available Since

Oct. 21, 2015

Availability

Academic Institutions and Nonprofits only

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pU6-26 (GB1001)

Plasmid

#68208

Purpose

Provides the A. thaliana U6-26 RNA polIII promoter as a level 0 GoldenBraid part

Depositor

Diego Orzaez

Article

Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

Insert

AtU6-26 promoter

Use

CRISPR and Synthetic Biology

Mutation

BsaI and BsmBI sites removed

Available Since

Oct. 19, 2015

Availability

Academic Institutions and Nonprofits only

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pCMB-NUCr

Plasmid

#61169

Purpose

Fluorescent reporter for nucleus NLS-mCherry-GUS expressed under MtBCP1 promoter

Depositor

Maria Harrison

Article

Ivanov et al Plant J. 2014 Oct 20. doi: 10.1111/tpj.1

Insert

NLS-mCherry-GUS

Tags

mCherry

Expression

Plant

Promoter

MtBCP1

Available Since

Feb. 23, 2015

Availability

Academic Institutions and Nonprofits only

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NET97 pmRFP-N2 (641)

Plasmid

#61994

Purpose

mammalian expression of nuclear envelope transmembrane protein

Depositor

Eric Schirmer

Article

Korfali et al Mol Cell Proteomics. 2010 Dec;9(12):2571

Insert

AADACL1 (NCEH1 Human)

Tags

mRFP

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 28, 2015

Availability

Academic Institutions and Nonprofits only
EGFP-bSV-171-1792

Plasmid

#41661

Depositor

Elizabeth Luna

Article

Wulfkuhle et al J Cell Sci. 1999 Jul:112 (Pt 13):2125-36

Insert

supervillin (SVIL Bovine)

Tags

EGFP

Expression

Mammalian

Mutation

amino acids 171-1792

Available Since

Dec. 12, 2012

Availability

Academic Institutions and Nonprofits only
TAL2473

Plasmid

#36854

Depositor

Keith Joung

Article

Reyon et al Nat Biotechnol. 2012 Apr 8. doi: 10.1038

Insert

CCND1-TALEN_Right (Ccnd1 Human)

Use

Mammalian, t7 for in vitro mrna synthesis

Tags

3X Flag and WT FOKI

Promoter

CMV

Available Since

June 26, 2012

Availability

Academic Institutions and Nonprofits only
TAL2304

Plasmid

#36733

Depositor

Keith Joung

Article

Reyon et al Nat Biotechnol. 2012 Apr 8. doi: 10.1038

Insert

CCND1-TALEN_Left (Ccnd1 Human)

Use

Mammalian, t7 for in vitro mrna synthesis

Tags

3X Flag and WT FOKI

Promoter

CMV

Available Since

June 26, 2012

Availability

Academic Institutions and Nonprofits only
TAL2246

Plasmid

#36691

Depositor

Keith Joung

Article

Reyon et al Nat Biotechnol. 2012 Apr 8. doi: 10.1038

Insert

PHF8-TALEN_Left (Phf8 Human)

Use

Mammalian, t7 for in vitro mrna synthesis

Tags

3X Flag and WT FOKI

Promoter

CMV

Available Since

June 26, 2012

Availability

Academic Institutions and Nonprofits only
pEMS1098

Plasmid

#29029

Purpose

Expression of the reporter gene under the control of the MiniPromoter in this plasmid has not been tested

Depositor

Elizabeth Simpson

Article

Portales-Casamar et al Proc Natl Acad Sci U S A. 2010 Sep 21. 1

Insert

Ple202 (SLC6A5 Human)

Use

Pleiades promoter project [sic, pleaides plieades]

Tags

EGFP-Cre-NLS

Available Since

Jan. 9, 2012

Availability

Academic Institutions and Nonprofits only

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pEMS1096

Plasmid

#29027

Purpose

Expression of the reporter gene under the control of the MiniPromoter in this plasmid has not been tested

Depositor

Elizabeth Simpson

Article

Portales-Casamar et al Proc Natl Acad Sci U S A. 2010 Sep 21. 1

Insert

Ple200 (SLC6A5 Human)

Use

Pleiades promoter project [sic, pleaides plieades]

Tags

EGFP-Cre-NLS

Available Since

Jan. 9, 2012

Availability

Academic Institutions and Nonprofits only

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SULT4A1

Plasmid

#25480

Purpose

Bacterial expression for structure determination; may not be full ORF

Depositor

Cheryl Arrowsmith

Insert

SULT4A1 (SULT4A1 Human)

Tags

His

Expression

Bacterial

Available Since

Aug. 23, 2010

Availability

Industry, Academic Institutions, and Nonprofits
pRS414 GPD-Sis1deltaG/F

Plasmid

#18688

Depositor

Susan Lindquist

Article

Douglas et al Proc Natl Acad Sci U S A. 2008 May 20. 1

Insert

Sis1 deltaG/F

Expression

Yeast

Mutation

Sis1 with the G/F region deleted

Available Since

June 11, 2008

Availability

Academic Institutions and Nonprofits only
XP1 Xwnt-1

Plasmid

#16990

Depositor

Randall Moon

Article

Wolda et al Dev Biol. 1993 Jan. 155(1):46-57.

Insert

Xwnt-1 (wnt1.L Frog)

Expression

Bacterial

Available Since

Feb. 1, 2008

Availability

Academic Institutions and Nonprofits only
pBS/KS-mId1sp

Plasmid

#16043

Depositor

Robert Benezra

Article

Jen et al Dev Dyn. 1996 Nov . 207(3):235-52.

Insert

Id1 (Id1 Mouse)

Expression

Bacterial

Mutation

cDNA nucleotides 390-742

Available Since

Nov. 2, 2007

Availability

Academic Institutions and Nonprofits only
pFbx15(-701/+114)-Luc

Plasmid

#13409

Depositor

Shinya Yamanaka

Article

Tokuzawa et al Mol Cell Biol. 2003 Apr . 23(8):2699-708

Insert

Upstream region of mouse Fbx15 gene

Use

Luciferase

Expression

Mammalian

Available Since

Jan. 12, 2007

Availability

Academic Institutions and Nonprofits only
pFbx15(-640/-527)-TK-Luc

Plasmid

#13413

Depositor

Shinya Yamanaka

Article

Tokuzawa et al Mol Cell Biol. 2003 Apr . 23(8):2699-708

Insert

Upstream region of mouse Fbx15 gene

Use

Luciferase

Expression

Mammalian

Available Since

Jan. 12, 2007

Availability

Academic Institutions and Nonprofits only
pAAV Jph3gRNA mEmerald hSyn mTagBFP2-CAAX2

Plasmid

#236246

Purpose

Endogenous tagging of Junctophilin 3 with mEmerald with a fluorescent marker for the plasma membrane

Depositor

Jennifer Lippincott-Schwartz

Article

Benedetti et al Cell. 2025 Jan 23;188(2):484-500.e22. do

Insert

gRNA for rat Jph3, mEmerald donor and mTagBFP2-CAAX2

Use

AAV

Tags

mTagBFP2

Promoter

U6 and human Synapsin1

Available Since

May 1, 2025

Availability

Academic Institutions and Nonprofits only

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pCAGGS-mNG-eDHFR(69K6)-G-alpha-q

Plasmid

#172106

Purpose

Mammalian expression of Gαq fused to mNeonGreen-eDHFR(69K6)

Depositor

Shinya Tsukiji

Article

Suzuki et al Cell Chem Biol. 2022 Sep 15;29(9):1446-1

Insert

mNeonGreen-eDHFR(69K6)-Gαq (GNAQ Human)

Expression

Mammalian

Mutation

Gαq: C9S/C10S/Q209L

Promoter

CAG

Available Since

July 14, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3-AktAR-Kras

Plasmid

#123357

Purpose

FRET-based biosensor for monitoring Akt activity in non-raft plasma membrane regions.

Depositor

Jin Zhang

Article

Gao et al Mol Biol Cell. 2008 Oct;19(10):4366-73.

Insert

AktAR-Kras

Tags

6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 3, 2021

Availability

Academic Institutions and Nonprofits only

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pET20b_Pa-PiuA

Plasmid

#128955

Purpose

expresses Pseudomonas aeruginosa PiuA in E. coli

Depositor

James Naismith

Article

Moynie et al Antimicrob Agents Chemother. 2017 Mar 24

Insert

iron transport outer membrane receptor (PiuA) (PA4514 Pseudomonas aeruginosa PAO1)

Tags

6xHis and pelB leader

Expression

Bacterial

Mutation

Signal peptide sequence has been removed

Promoter

T7

Available Since

Aug. 26, 2019

Availability

Academic Institutions and Nonprofits only

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pVENUS-N1-Ubl4A

Plasmid

#86857

Purpose

To express Ubl4A tagged with VENUS at C terminus.

Depositor

Yihong Ye

Article

Xu et al Cell Discov. 2016 Nov 8;2:16040. eCollec

Insert

UBL4A (UBL4A Human)

Tags

VENUS

Expression

Mammalian

Mutation

none

Promoter

CMV

Available Since

April 5, 2017

Availability

Academic Institutions and Nonprofits only

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FLAG-SLC30A10-E25A

Plasmid

#82345

Purpose

Expresses FLAG-tagged SLC30A10-E25A in mammalian cells

Depositor

Somshuvra Mukhopadhyay

Article

Zogzas et al J Biol Chem. 2016 Jul 29;291(31):15940-5

Insert

SLC30A10 (SLC30A10 Human)

Tags

3xFLAG

Expression

Mammalian

Mutation

E25A

Promoter

CMV

Available Since

Sept. 12, 2016

Availability

Academic Institutions and Nonprofits only

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pcDNA5 FRT/TO Plk4 KD ∆24-mCherry

Plasmid

#80272

Purpose

mammalian expression plasmid for kinase dead Plk4 with multi-phospho degron deleted

Depositor

Andrew Holland

Article

Moyer et al J Cell Biol. 2015 Jun 22;209(6):863-78.

Insert

Plk4 (PLK4 Human)

Tags

mCherry

Expression

Mammalian

Mutation

deletion of multi-phospho degron (amino acids 282…

Promoter

CMV

Available Since

Aug. 2, 2016

Availability

Academic Institutions and Nonprofits only

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pSP64-hERG1a T623A

Plasmid

#53055

Purpose

Expresses alpha subunit of T623A hERG1a K channel in Xenopus oocytes

Depositor

Michael Sanguinetti

Article

Mitcheson et al Proc Natl Acad Sci U S A. 2000 Oct 24;97

Insert

Potassium voltage-gated channel subfamily H member 2 (KCNH2 Human)

Use

Xenopus oocyte expression

Mutation

changed Threonine 623 to Alanine

Promoter

SP6

Available Since

June 9, 2014

Availability

Academic Institutions and Nonprofits only

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pCRII-Topo Couptf1

Plasmid

#45870

Depositor

Jeffrey Macklis

Insert

Couptf1 in situ probe (Nr2f1 Mouse)

Use

In situ

Mutation

contains bp# 1755-2381 of NM_010151.2

Available Since

July 9, 2013

Availability

Academic Institutions and Nonprofits only
pcDNA3 PARL-FLAG-CT L79E

Plasmid

#13640

Depositor

Luca Pellegrini

Article

Sik et al J Biol Chem. 2004 Apr 9. 279(15):15323-9

Insert

Presenilin-associated rhomboid-like (PARL, PARL Human)

Tags

FLAG

Expression

Mammalian

Mutation

changed Leu 79 to Glu

Available Since

Jan. 4, 2007

Availability

Academic Institutions and Nonprofits only
p2xp53_RE

Plasmid

#118053

Purpose

GBPart - Operator/DNA Response element - 2 copies of the p53 DNA binding motif

Depositor

Koen Venken

Article

Sarrion-Perdigones et al Nat Commun. 2019 Dec 13;10(1):5710. doi:

Insert

2 copies of the p53 DNA binding motif

Use

Synthetic Biology; Domestication of dna parts for…

Available Since

Dec. 16, 2019

Availability

Academic Institutions and Nonprofits only

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pLaNGo11-7

Plasmid

#126055

Purpose

A self-assembling split-GFP based Golden Gate vector to determine protein targeting to plastids in plant cell

Depositor

Ralf-Bernd Klösgen

Article

Sharma et al J Cell Sci. 2019 Jun 3;132(11). pii: jcs

Inserts

chloroplastic FNR transit peptide
ccdB cassette (BsaI)
GFP11x7 (5 aa linker)

Use

Binary

Tags

GFP1-10

Expression

Plant

Available Since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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pLV-EF1a-V5-Sec61B

Plasmid

#120244

Purpose

Expresses V5-tagged Sec61B in lentiviral vector

Depositor

Larry Gerace

Article

Cheng et al Nucleus. 2019 Dec;10(1):126-143. doi: 10

Insert

Sec61B (Sec61b Mouse)

Use

Lentiviral

Tags

V5

Promoter

EF1a

Available Since

Jan. 9, 2019

Availability

Academic Institutions and Nonprofits only

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pFL His-SDS22 / PP1gamma

Plasmid

#113511

Purpose

Expression of His-SDS22 / PP1gamma dimer in insect cells (Baculovirus system)

Depositor

Hemmo Meyer

Article

Weith et al Mol Cell. 2018 Oct 11. pii: S1097-2765(1

Inserts

SDS22 (PPP1R7 Human)
PP1gamma (PPP1CC Human)

Tags

6*His

Expression

Insect

Available Since

Oct. 11, 2018

Availability

Academic Institutions and Nonprofits only

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pET28a-SpyTag002 DA-MBP

Plasmid

#102832

Purpose

Bacterial expression of unreactive version of SpyTag002 fused to maltose binding protein

Depositor

Mark Howarth

Article

Keeble et al Angew Chem Int Ed Engl. 2017 Oct 10. doi

Insert

SpyTag002 DA-MBP

Tags

His6 and Thrombin cleavage site

Expression

Bacterial

Mutation

Reactive aspartic acid has been mutated to an ala…

Promoter

T7

Available Since

Dec. 21, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pMDC43-FIT2

Plasmid

#96993

Purpose

Express GFP-tagged mouse FIT2 gene in plants.

Depositor

Kent Chapman

Article

Cai et al Plant Biotechnol J. 2016 Dec 17. doi: 10

Insert

MmFIT2 (Fitm2 Mouse)

Tags

GFP

Expression

Plant

Mutation

E2D (please see depositor comments below)

Promoter

35S

Available Since

Aug. 17, 2017

Availability

Academic Institutions and Nonprofits only

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pFBD-Akt1(T308A/S473A)

Plasmid

#86578

Purpose

Expresses human full-length Akt1(T308A/S473A) phosphomutant

Depositor

Thomas Leonard, Ivan Yudushkin

Article

Ebner et al Mol Cell. 2017 Feb 2;65(3):416-431.e6. d

Insert

Akt1 (AKT1 Human)

Tags

10xHis

Expression

Insect

Mutation

T308A/S473A phosphomutant

Available Since

April 4, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-mGnptg

Plasmid

#78106

Purpose

Mammalian expression of mouse Gnptg with C-terminal His tag

Depositor

Thomas Braulke

Article

Encarnacao et al J Biol Chem. 2011 Feb 18;286(7):5311-8.

Insert

Gnptg (Gnptg Mouse)

Tags

His

Expression

Mammalian

Promoter

CMV

Available Since

May 19, 2016

Availability

Academic Institutions and Nonprofits only
GFP SRBC

Plasmid

#68399

Purpose

Expression of fluorescently tagged SRBC in mammalian cells

Depositor

Rob Parton

Article

Bastiani et al J Cell Biol. 2009 Jun 29;185(7):1259-73.

Insert

SRBC (Cavin3 Mouse)

Tags

GFP

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 18, 2015

Availability

Academic Institutions and Nonprofits only
pCRII-Sema4a-ISH-2kb

Plasmid

#68029

Purpose

Contains 2kb cDNA fragment for probe synthesis for mRNA in situ hybridization, species: mouse

Depositor

Roland Friedel

Article

Maier et al Mol Cell Neurosci. 2011 Feb;46(2):419-31

Insert

Semaphorin-4A (Sema4a Mouse)

Use

Mrna in situ hybridization probe

Mutation

a 2kb cDNA fragment for ISH probe synthesis

Available Since

Aug. 27, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
STT3A pmRFP (663)

Plasmid

#61997

Purpose

mammalian expression of nuclear envelope transmembrane protein

Depositor

Eric Schirmer

Article

Korfali et al Mol Cell Proteomics. 2010 Dec;9(12):2571

Insert

STT3A (STT3A Human)

Tags

mRFP

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 28, 2015

Availability

Academic Institutions and Nonprofits only
pDest27-mBIN1+17

Plasmid

#61096

Purpose

Express N-terminal GST tagged-mouse BIN1+17 in mammalian cells

Depositor

Robin Shaw

Article

Hong et al Nat Med. 2014 Jun;20(6):624-32. doi: 10.

Insert

mouse BIN1 with exon 17, excluding exon 7, 11, 13-16

Tags

GST

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 23, 2015

Availability

Academic Institutions and Nonprofits only
Mesp-1916/-1>nls::Cas9::nls

Plasmid

#59988

Purpose

Mesp driver driving nls::Cas9::nls

Depositor

Lionel Christiaen

Article

Stolfi et al Development. 2014 Nov;141(21):4115-20. d

Insert

nls::Cas9::nls

Use

CRISPR

Mutation

Reverted mutations from dCas9 to wiltdtype

Promoter

Ciinte.Mesp -1916/-1

Available Since

Nov. 18, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
EGFP-bSV-1282-1792

Plasmid

#40960

Depositor

Elizabeth Luna

Article

Wulfkuhle et al J Cell Sci. 1999 Jul:112 (Pt 13):2125-36

Insert

supervillin (SVIL Bovine)

Tags

EGFP

Expression

Mammalian

Mutation

deleted amino acids 1-1281

Available Since

Nov. 30, 2012

Availability

Academic Institutions and Nonprofits only
TAL2386

Plasmid

#36795

Depositor

Keith Joung

Article

Reyon et al Nat Biotechnol. 2012 Apr 8. doi: 10.1038

Insert

CYLD-TALEN_Left (Cyld Human)

Use

Mammalian, t7 for in vitro mrna synthesis

Tags

3X Flag and WT FOKI

Promoter

CMV

Available Since

June 26, 2012

Availability

Academic Institutions and Nonprofits only
TAL2358

Plasmid

#36771

Depositor

Keith Joung

Article

Reyon et al Nat Biotechnol. 2012 Apr 8. doi: 10.1038

Insert

CBX3-TALEN_Left (CBX3 Human)

Use

Mammalian, t7 for in vitro mrna synthesis

Tags

3X Flag and WT FOKI

Promoter

CMV

Available Since

June 26, 2012

Availability

Academic Institutions and Nonprofits only
TAL2359

Plasmid

#36772

Depositor

Keith Joung

Article

Reyon et al Nat Biotechnol. 2012 Apr 8. doi: 10.1038

Insert

CBX3-TALEN_Right (CBX3 Human)

Use

Mammalian, t7 for in vitro mrna synthesis

Tags

3X Flag and WT FOKI

Promoter

CMV

Available Since

June 26, 2012

Availability

Academic Institutions and Nonprofits only
pUb-Cas9-EGFP_cU6:6-sgRNA

Plasmid

#190597

Purpose

The plasmid encodes S. pyogenes Cas9 and eGFP separated by a T2A peptide under an Aedes aegypti polyubiquitin promoter. It also expresses a sgRNA scaffold under a Culex quinquefasciatus U6 promoter.

Depositor

Claudia Rueckert

Article

Torres et al Insects. 2022 Sep 19;13(9):856. doi: 10.

Inserts

Cas9
sgRNA

Use

CRISPR

Tags

EGFP - separated by T2A

Promoter

Aedes aegypti polyubiquitin and Culex quinquefasc…

Available Since

Jan. 17, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pOTTC710 - pAAV EF1a DIO Mem-AcGFP

Plasmid

#75081

Purpose

An AAV packaging vector that expresses Cre-dependent membrane-localized AcGFP under control of the EF1a promoter.

Depositor

Brandon Harvey

Article

Sharpe et al Curr Biol. 2017 Jul 24;27(14):2089-2100.

Insert

Mem-AcGFP

Use

AAV and Cre/Lox

Tags

Palmitylation site

Expression

Mammalian

Promoter

EF1a

Available Since

Jan. 18, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
Kv7.4 (1-645, Δ368-523)/HA tag/pcDNA3.1

Plasmid

#111459

Purpose

Electrophysiology in HEK cell

Depositor

Dan Minor

Article

Chang et al Neuron. 2018 Feb 21;97(4):836-852.e6. do

Insert

potassium voltage-gated channel subfamily KQT member 4 isoform a [Homo sapiens] (KCNQ4 Human)

Tags

Ha Tag

Expression

Mammalian

Mutation

Kv7.4 (1-645, Δ368-523), C643A

Available Since

Oct. 31, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge

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