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pTfdc1Sc

Plasmid

#78287

Purpose

Expresses S. cerevisiae FDC1 in E. coli

Depositor

David Nielsen

Article

McKenna et al Metab Eng. 2011 Sep;13(5):544-54. doi: 1

Insert

FDC1 (FDC1 Budding Yeast)

Expression

Bacterial

Promoter

trc

Available Since

June 28, 2016

Availability

Academic Institutions and Nonprofits only

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pDS3-X4

Plasmid

#70140

Purpose

Yeast chr. integration vector with DS3 selection marker

Depositor

Morten Sommer

Article

Rugbjerg et al Metab Eng. 2015 Sep;31:189-97. doi: 10.1

Insert

DS3

Expression

Yeast

Promoter

AgTEF1

Available Since

Dec. 23, 2015

Availability

Academic Institutions and Nonprofits only

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pYD1

Plasmid

#73447

Purpose

expression vector designed for expression, secretion, and display of proteins on the extracellular surface of Saccharomyces cerevisiae cells

Depositor

Dane Wittrup

Article

Kieke et al Protein Eng. 1997 Nov;10(11):1303-10.

Type

Empty backbone

Tags

6xHis, AGA2, T7 peptide, V5 tag, and Xpress tag

Expression

Yeast

Promoter

GAL1

Available Since

Feb. 16, 2016

Availability

Academic Institutions and Nonprofits only

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pTrcECT

Plasmid

#75477

Purpose

Inducible expression of ectABC in E. coli

Depositor

Xixian Xie

Article

Ning et al Metab Eng. 2016 Mar 9;36:10-18. doi: 10.

Insert

ectABC

Expression

Bacterial

Promoter

trc

Available Since

May 17, 2016

Availability

Academic Institutions and Nonprofits only

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pBEST-OR2-OR1-Pr-UTR1-deGFP-T500

Plasmid

#40019

Depositor

Vincent Noireaux

Article

Shin et al J Biol Eng. 2010 Jun 24;4:8.

Insert

deGFP

Expression

Bacterial

Promoter

OR2-OR1-Pr (bacteriophage Lambda with one mutatio…

Available Since

Oct. 1, 2012

Availability

Academic Institutions and Nonprofits only

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pIYC08

Plasmid

#64743

Purpose

Yeast expression plasmid: acsL641P, ALD6, ADH2, ERG10

Depositor

Jens Nielsen

Article

Chen et al Metab Eng. 2013 Jan;15:48-54. doi: 10.10

Insert

ADH2, ALD6, acsL641P, ERG10

Expression

Yeast

Available Since

July 28, 2015

Availability

Academic Institutions and Nonprofits only

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pEEK2

Plasmid

#83492

Purpose

Expression plasmid for conjugational transfer into cyanobacteria

Depositor

Pia Lindberg

Article

Miao et al Metab Eng Commun. 2017 Jul 29;5:45-53. d

Type

Empty backbone

Use

Synthetic Biology

Tags

Strep-tag

Expression

Bacterial

Promoter

Ptrc core

Available Since

Nov. 29, 2018

Availability

Academic Institutions and Nonprofits only

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pENTR-p14FAST

Plasmid

#50025

Purpose

pENTR vector for reptilian orthoreovirus p14 fusion-associated small transmembrane (FAST) protein

Depositor

Jamie Davies

Article

Cachat et al J Biol Eng. 2014 Nov 19;8(1):26. eCollec

Insert

p14FAST

Use

Entry vector

Available Since

Dec. 10, 2014

Availability

Academic Institutions and Nonprofits only

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pBA2kIKmA2

Plasmid

#39213

Depositor

Anastasios Melis

Article

Lindberg et al Metab Eng. 2010 Jan;12(1):70-9. Epub 200

Insert

Kudzu Isoprene Synthase

Use

Synthetic Biology

Mutation

Deleted the predicted chloroplast transit peptide

Promoter

psbA2

Available Since

Aug. 16, 2012

Availability

Academic Institutions and Nonprofits only
pSpal2At

Plasmid

#78286

Purpose

Expresses A. thaliana PAL2 in E. coli

Depositor

David Nielsen

Article

McKenna et al Metab Eng. 2011 Sep;13(5):544-54. doi: 1

Insert

PAL2 (PAL2 Mustard Weed)

Expression

Bacterial

Mutation

*see below

Promoter

lac

Available Since

June 1, 2016

Availability

Academic Institutions and Nonprofits only

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cp196V1.2

Plasmid

#52100

Purpose

A improved cpmCherry variant

Depositor

Robert Campbell

Article

Carlson et al Protein Eng Des Sel. 2013 Dec;26(12):763

Insert

cp196V1.2

Tags

6 His tag

Expression

Bacterial

Promoter

pBAD promotor

Available Since

April 14, 2014

Availability

Academic Institutions and Nonprofits only
JBEI-12050

Plasmid

#84982

Purpose

Isopentenol production

Depositor

Taek Soon Lee

Article

Kang et al Metab Eng. 2016 Mar;34:25-35. doi: 10.10

Insert

MevTo-T21-MK-aphA

Expression

Bacterial

Promoter

LacUV5

Available Since

July 31, 2017

Availability

Academic Institutions and Nonprofits only

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pAC-PHYT

Plasmid

#19002

Depositor

Jay Keasling

Article

Smolke et al Metab Eng. 2001 Oct . 3(4):313-21.

Insert

crtE, crtB

Use

Synthetic Biology

Expression

Bacterial

Available Since

Oct. 27, 2008

Availability

Academic Institutions and Nonprofits only
pIVEX-anchor

Plasmid

#46844

Purpose

Template for the amplification of spiking anchors in BeSD

Depositor

Florian Hollfelder

Article

Diamante et al Protein Eng Des Sel. 2013 Oct;26(10):713

Type

Empty backbone

Use

In vitro expression

Available Since

Aug. 13, 2013

Availability

Academic Institutions and Nonprofits only

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JBEI-9348

Plasmid

#84981

Purpose

Isopentenol production

Depositor

Taek Soon Lee

Article

Kang et al Metab Eng. 2016 Mar;34:25-35. doi: 10.10

Insert

PMD-NudB

Expression

Bacterial

Promoter

trc promoter

Available Since

Aug. 1, 2017

Availability

Academic Institutions and Nonprofits only

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JBEI-9314

Plasmid

#84979

Purpose

Isopentenol production

Depositor

Taek Soon Lee

Article

Kang et al Metab Eng. 2016 Mar;34:25-35. doi: 10.10

Insert

PMD

Expression

Bacterial

Promoter

trc promoter

Available Since

Aug. 1, 2017

Availability

Academic Institutions and Nonprofits only

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PHBA01

Plasmid

#83535

Purpose

Constitutive TEF1 promoter mediated expression of the UBiC gene

Depositor

Claudia Vickers

Article

Williams et al Metab Eng Commun. 2016 May 11;3:142-152.

Insert

pTEF1-UBiC-CYC1t

Expression

Yeast

Promoter

pTEF1

Available Since

Oct. 5, 2016

Availability

Academic Institutions and Nonprofits only

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pDS2-aurZ

Plasmid

#70151

Purpose

Fusarium graminearum AurZ encoded on CEN/ARS yeast shuttle vector with DS2 selection marker

Depositor

Morten Sommer

Article

Rugbjerg et al Metab Eng. 2015 Sep;31:189-97. doi: 10.1

Insert

aurZ

Expression

Yeast

Promoter

Tef

Available Since

Jan. 7, 2016

Availability

Academic Institutions and Nonprofits only

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pMSP2N2

Plasmid

#29520

Depositor

Stephen Sligar

Article

Grinkova et al Protein Eng Des Sel. 2010 Nov . 23(11):8

Insert

MSP2N2

Tags

7-His tag

Expression

Bacterial

Mutation

tandem MSP; His-tag on N-terminus followed by spa…

Available Since

May 3, 2011

Availability

Academic Institutions and Nonprofits only

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pBbS2k-RFP

Plasmid

#35330

Depositor

Jay Keasling

Article

Lee et al J Biol Eng. 2011 Sep 20;5:12.

Insert

RFP

Use

Synthetic Biology

Expression

Bacterial

Promoter

Tet

Available Since

Feb. 20, 2012

Availability

Academic Institutions and Nonprofits only

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