We narrowed to 50,371 results for: Son

1. 14x PP7 with loxP-kanMX-loxP

   Plasmid

   #189939

   Purpose

   Integration of 14xPP7 loops in Saccharomyces cerevisiae. Selection by KanMX (flanked by LoxP sites for marker removal)

   Depositor

   Tineke Lenstra

   Article

   Donovan et al EMBO J. 2019 Jun 17;38(12). pii: embj.20

   Insert

   14x PP7 with loxP-kanMX-loxP
   

   Use

   Tags

   kanMX

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Feb. 14, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. mEmerald-MyosinIIA-N-14

   Plasmid

   #54191

   Purpose

   Localization: Cytoskeleton, Excitation: 487, Emission: 509

   Depositor

   Michael Davidson

   Article

   Burnette et al J Cell Biol. 2014 Apr 14;205(1):83-96. d

   Insert

   MyosinIIA
   

   Use

   Tags

   mEmerald

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   July 25, 2014

   Availability

   Industry, Academic Institutions, and Nonprofits

3. pEJS2705-CMV-Nme2Smu-ABE8e-i8-L10-D56R

   Plasmid

   #228726

   Purpose

   Expresses domain inlaid Nme2Smu nCas9 (D16A)-i8-ABE8e base editor with linker-10 and D56R mutation in mammalian cells

   Depositor

   Erik Sontheimer

   Article

   Bamidele et al bioRxiv November 07, 2024

   Insert

   Domain inlaid Nme2Smu nCas9 (D16A)-i8-L10-D56R
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Nme2-Cas9, Smu PID, D16A D56R

   Promoter

   CMV

   Available since

   Nov. 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pEJS2704-CMV-Nme2Smu-ABE8e-i8-L10-E932R

   Plasmid

   #228725

   Purpose

   Expresses domain inlaid Nme2Smu nCas9 (D16A)-i8-ABE8e base editor with linker-10 and E932R mutation in mammalian cells

   Depositor

   Erik Sontheimer

   Article

   Bamidele et al bioRxiv November 07, 2024

   Insert

   Domain inlaid Nme2Smu nCas9 (D16A)-i8-L10-E932R
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Nme2-Cas9, Smu PID, D16A E932R

   Promoter

   CMV

   Available since

   Nov. 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pEJS2708-CMV-Nme2Smu-ABE8e-i1-L10-E932R

   Plasmid

   #228729

   Purpose

   Expresses domain inlaid Nme2Smu nCas9 (D16A)-i1-ABE8e base editor with linker-10 and E932R mutation in mammalian cells

   Depositor

   Erik Sontheimer

   Article

   Bamidele et al bioRxiv November 07, 2024

   Insert

   Domain inlaid Nme2Smu nCas9 (D16A)-i1-L10-E932R
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Nme2-Cas9, Smu PID, D16A E932R

   Promoter

   CMV

   Available since

   Nov. 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pEJS2706-CMV-Nme2-ABE8e-i8-L10-E932R

   Plasmid

   #228727

   Purpose

   Expresses domain inlaid Nme2 nCas9 (D16A)-i8-ABE8e base editor with linker-10 and E932R mutation in mammalian cells

   Depositor

   Erik Sontheimer

   Article

   Bamidele et al bioRxiv November 07, 2024

   Insert

   Domain inlaid Nme2 nCas9 (D16A)-i8-L10-E932R
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Nme2-Cas9 D16A E932R

   Promoter

   CMV

   Available since

   Nov. 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pEJS2707-CMV-Nme2-ABE8e-i8-L10-D56R

   Plasmid

   #228728

   Purpose

   Expresses domain inlaid Nme2 nCas9 (D16A)-i8-ABE8e base editor with linker-10 and D56R mutation in mammalian cells

   Depositor

   Erik Sontheimer

   Article

   Bamidele et al bioRxiv November 07, 2024

   Insert

   Domain inlaid Nme2 nCas9 (D16A)-i8-L10-D56R
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Nme2-Cas9 D16A D56R

   Promoter

   CMV

   Available since

   Nov. 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pEJS2709-CMV-Nme2Smu-ABE8e-i1-L10-D56R

   Plasmid

   #228730

   Purpose

   Expresses domain inlaid Nme2Smu nCas9 (D16A)-i1-ABE8e base editor with linker-10 and D56R mutation in mammalian cells

   Depositor

   Erik Sontheimer

   Article

   Bamidele et al bioRxiv November 07, 2024

   Insert

   Domain inlaid Nme2Smu nCas9 (D16A)-i1-L10-D56R
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Nme2-Cas9, Smu PID, D16A D56R

   Promoter

   CMV

   Available since

   Nov. 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pENTRY-YFP

   Plasmid

   #15302

   Depositor

   William Hahn

   Article

   Boehm et al Cell. 2007 Jun 15. 129(6):1065-1079.

   Insert

   YFP
   

   Use

   Gateway entry

   Tags

   Expression

   Mutation

   Promoter

   Available since

   July 12, 2007

   Availability

   Academic Institutions and Nonprofits only

10. 2XMyc-LRRK2-K1347A

    Plasmid

    #25365

    Depositor

    Mark Cookson

    Article

    J Biol Chem. 2008 Jun 13;283(24):16906-1

    Insert

    LRRK2 (LRRK2 Human)
    

    Use

    Tags

    2XMyc

    Expression

    Mammalian

    Mutation

    Lysine 1347 mutated to Alanine

    Promoter

    Available since

    June 22, 2010

    Availability

    Academic Institutions and Nonprofits only

11. pCR1446

    Plasmid

    #111129

    Purpose

    Expresses FANCF

    Depositor

    Jacob Corn

    Article

    Richardson et al Nat Genet. 2018 Aug;50(8):1132-1139. doi

    Insert

    FANCF (FANCF Human)
    

    Use

    Lentiviral

    Tags

    HA

    Expression

    Mutation

    Promoter

    Available since

    Oct. 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pTol1:uas-myc-act-map2k2b-2a-tdTomato

    Plasmid

    #104721

    Purpose

    UAS-driven expression of activated MEK

    Depositor

    Nathan Lawson

    Article

    Shin et al Development. 2016 Oct 15;143(20):3785-37

    Insert

    activated map2k2b (map2k2b Zebrafish)
    

    Use

    Zebrafish transposon

    Tags

    2A-tdTomato and myc tag

    Expression

    Mutation

    serines 221 and 225 to aspartic acid

    Promoter

    10xUAS

    Available since

    Jan. 4, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pEGFP C1 SADBs

    Plasmid

    #67154

    Purpose

    pEGFP containing N-terminally EGFP Tagged mouse SADBs

    Depositor

    Maria Alvarado-Kristensson, Ana Carrera

    Article

    Alvarado-Kristensson et al Nat Cell Biol. 2009 Sep;11(9):1081-92. d

    Insert

    Brsk1 (Brsk1 Mouse)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 8, 2015

    Availability

    Academic Institutions and Nonprofits only

14. miniSOG-RatLC3

    Plasmid

    #57775

    Purpose

    Localization: Microtubule-Binding Protein, Excitation: 448 / 473, Emission: 500 / 528

    Depositor

    Michael Davidson

    Article

    Shu et al PLoS Biol. 2011 Apr;9(4):e1001041. doi:

    Insert

    LC3
    

    Use

    Tags

    miniSOG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 5, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

15. mRuby2-PMP-N-10

    Plasmid

    #55910

    Purpose

    Localization: Peroxisomes, Excitation: 559, Emission: 600

    Depositor

    Michael Davidson

    Article

    Lam et al Nat Methods. 2012 Sep 9. doi: 10.1038/nm

    Insert

    PMP (PXMP2 Human)
    

    Use

    Tags

    mRuby2

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 12, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

16. eGFP-HA_BASU

    Plasmid

    #153996

    Purpose

    Express eGFP gene along with HA and BASU

    Depositor

    Paul Khavari

    Article

    Meyers et al PLoS Pathog. 2021 Oct 1;17(10):e1009412.

    Insert

    E-GFP
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 5, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pQCXIP Flag-hRpt6

    Plasmid

    #19793

    Depositor

    Ronald Conaway, Joan Conaway

    Article

    Yao et al Mol Cell. 2008 Sep 26. 31(6):909-17.

    Insert

    hRpt6 (PSMC5 Human)
    

    Use

    Retroviral

    Tags

    Flag

    Expression

    Mutation

    Promoter

    Available since

    Nov. 21, 2008

    Availability

    Academic Institutions and Nonprofits only

18. SuExp-mPLD3

    Plasmid

    #201245

    Purpose

    express full length mouse PLD3 protein

    Depositor

    David Nemazee

    Article

    Yuan et al Structure. 2024 Jun 6;32(6):766-779.e7.

    Insert

    PLD3 (Pld3 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. 2XMyc-LRRK2-RQ/TG

    Plasmid

    #25369

    Depositor

    Mark Cookson

    Article

    J Biol Chem. 2008 Jun 13;283(24):16906-1

    Insert

    LRRK2 (LRRK2 Human)
    

    Use

    Tags

    2XMyc

    Expression

    Mammalian

    Mutation

    Threonine 1343 mutated to Glycine Arginine 139…

    Promoter

    Available since

    June 29, 2010

    Availability

    Academic Institutions and Nonprofits only

20. pDSP15 (5’Δ HygR + loxP + MCS + 5’Δ mScarlet-I)

    Plasmid

    #193853

    Purpose

    Promoterless insertion vector for split HygR/mScarlet-I landing pad

    Depositor

    Patrick Phillips

    Article

    Stevenson et al Elife. 2023 Jul 4;12:RP84831. doi: 10.75

    Insert

    5' rps-0::HygR + 5' mScarlet-I
    

    Use

    CRISPR and Cre/Lox

    Tags

    Expression

    Worm

    Mutation

    Promoter

    rps-0p

    Available since

    Sept. 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pDOC-GG

    Plasmid

    #149377

    Purpose

    Chassis plasmid for building mutagenesis cassettes by Golden Gate assembly to form a donor plasmid for recombineering by Gene Doctoring

    Depositor

    Mark Pallen

    Article

    Thomson et al BMC Biotechnol. 2020 Oct 7;20(1):54. doi

    Inserts

    Fragment containing a kanamycin resistance cassette
    Fragment containing pMB1 origin of replication and an I-SceI recognition sequence
    Fragment containing a lacZ⍺ expression cassette flanked by BsaI sites
    Fragment containing an I-SceI recognition sequence
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. mEos2-CD9-10

    Plasmid

    #57356

    Purpose

    Localization: Tetraspanin/Membrane, Excitation: 505 / 569, Emission: 516 / 581

    Depositor

    Michael Davidson

    Article

    McKinney et al Nat Methods. 2009 Feb . 6(2):131-3.

    Insert

    CD9 (CD9 Human)
    

    Use

    Tags

    mEos2

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 5, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

23. tdKatushka2-VASP-5

    Plasmid

    #56049

    Purpose

    Localization: Actin/Focal Adhesions, Excitation: 586, Emission: 632

    Depositor

    Michael Davidson

    Article

    Shcherbo et al Biochem J. 2009 Mar 15;418(3):567-74. do

    Insert

    VASP
    

    Use

    Tags

    tdKatushka2

    Expression

    Mammalian

    Mutation

    A209T in VASP

    Promoter

    CMV

    Available since

    April 1, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

24. PA-mCherry-miniSOG-Paxillin-22

    Plasmid

    #57796

    Purpose

    Localization: Focal Adhesions, Excitation: 448 / 473, Emission: 500 / 528

    Depositor

    Michael Davidson

    Article

    Shu et al PLoS Biol. 2011 Apr;9(4):e1001041. doi:

    Insert

    Paxillin
    

    Use

    Tags

    PA-mCherry-miniSOG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 5, 2015

    Availability

    Academic Institutions and Nonprofits only

25. AsCas12a-DR-cr in PBCSK-

    Plasmid

    #126640

    Purpose

    Cloning vector AsCas12a DR-crRNA for expression in mammalian cells

    Depositor

    Scot Wolfe

    Article

    Liu et al Nucleic Acids Res. 2019 Mar 20. pii: 540

    Insert

    AsCas12a Full Length Direct Repeat crRNA
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6 Promoter

    Available since

    June 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. PA-mCherry-miniSOG-Lysosomes-20

    Plasmid

    #57793

    Purpose

    Localization: Lysosome Membrane, Excitation: 448 / 473, Emission: 500 / 528

    Depositor

    Michael Davidson

    Article

    Shu et al PLoS Biol. 2011 Apr;9(4):e1001041. doi:

    Insert

    LAMP1
    

    Use

    Tags

    PA-mCherry-miniSOG

    Expression

    Mammalian

    Mutation

    D50E in LAMP1

    Promoter

    CMV

    Available since

    Jan. 29, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. 2XMyc-LRRK2-RCKW

    Plasmid

    #25064

    Depositor

    Mark Cookson

    Article

    J Biol Chem. 2008 Jun 13;283(24):16906-1

    Insert

    LRRK2 (LRRK2 Human)
    

    Use

    Tags

    2XMyc

    Expression

    Mammalian

    Mutation

    Contains only the ROC-COR-Kinase-WD40 domains …

    Promoter

    Available since

    June 29, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. EGFP-ER-14

    Plasmid

    #56432

    Purpose

    Localization: Endoplasmic Reticulum, Excitation: 488, Emission: 507

    Depositor

    Michael Davidson

    Article

    Day et al Chem Soc Rev. 2009 Oct;38(10):2887-921.

    Insert

    ER (CALR Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    ER Signal Peptide

    Promoter

    CMV

    Available since

    Dec. 23, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits

29. Clover-pBAD

    Plasmid

    #54575

    Purpose

    Localization: Protein Expression Vector , Excitation: 505, Emission: 515. This plasmid encodes Clover H231L based on reference from Bajar et. al. Sci Rep. 2016.

    Depositor

    Michael Davidson

    Article

    Lam et al Nat Methods. 2012 Sep 9. doi: 10.1038/nm

    Type

    Empty backbone

    Use

    Tags

    6xHis and Clover

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    July 25, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits

30. mTagBFP2-Caveolin-C-10

    Plasmid

    #55279

    Purpose

    Localization: Membrane, Excitation: 399, Emission: 456

    Depositor

    Michael Davidson

    Article

    Subach et al PLoS One. 2011;6(12):e28674. Epub 2011 D

    Insert

    Caveolin (CAV1 Human)
    

    Use

    Tags

    mTagBFP2

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 10, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits

31. pCDNA5/FRT/TO_H2B_HaloTag9

    Plasmid

    #169334

    Purpose

    Nuclear expression of HaloTag9 in mammalian cells

    Depositor

    Kai Johnsson

    Article

    Frei et al Nat Methods. 2021 Dec 16. pii: 10.1038/s

    Insert

    H2B-HaloTag9 (H2BC21 Human)
    

    Use

    Tags

    HaloTag9

    Expression

    Mammalian

    Mutation

    HaloTag9 = HaloTag7-Q165H-P174R

    Promoter

    Available since

    Nov. 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. 2XMyc-LRRK2-WD40

    Plasmid

    #25073

    Depositor

    Mark Cookson

    Article

    J Biol Chem. 2008 Jun 13;283(24):16906-1

    Insert

    LRRK2 (LRRK2 Human)
    

    Use

    Tags

    2XMyc

    Expression

    Mammalian

    Mutation

    Contains only the WD40 domain aa 2155-2527

    Promoter

    Available since

    Nov. 1, 2011

    Availability

    Academic Institutions and Nonprofits only

33. pBacT7-S1T1L

    Plasmid

    #33292

    Depositor

    Terence Dermody

    Article

    Kobayashi et al Cell Host Microbe. 2007 Apr 19;1(2):147-

    Insert

    S1 (MoV1sS1gp2, MoV1sS1gp1 Orthoreovirus T1L)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    T7

    Available since

    Dec. 5, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. mEmerald-MyosinIIA-C-18

    Plasmid

    #54190

    Purpose

    Localization: Cytoskeleton, Excitation: 487, Emission: 509

    Depositor

    Michael Davidson

    Article

    Burnette et al J Cell Biol. 2014 Apr 14;205(1):83-96. d

    Insert

    MyosinIIA
    

    Use

    Tags

    mEmerald

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 10, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits

35. Clover-PDHA1-N-10

    Plasmid

    #56304

    Purpose

    Localization: Mitochondria, Excitation: 505, Emission: 515

    Depositor

    Michael Davidson

    Article

    Lam et al Nat Methods. 2012 Sep 9. doi: 10.1038/nm

    Insert

    PDHA1 (PDHA1 Human)
    

    Use

    Tags

    Clover

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Dec. 5, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits

36. pET51b-His-TEV-HaloTag7_dead_mt

    Plasmid

    #167267

    Purpose

    Overexpression of the dead variant (D106A) of HaloTag7 in E. coli

    Depositor

    Kai Johnsson

    Article

    Wilhelm et al Biochemistry. 2021 Aug 24;60(33):2560-25

    Insert

    HaloTag7
    

    Use

    Tags

    His x10

    Expression

    Bacterial

    Mutation

    D106A

    Promoter

    T7

    Available since

    April 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. mEmerald-H2B-C-10

    Plasmid

    #54112

    Purpose

    Localization: Nucleus/Histones, Excitation: 487, Emission: 509

    Depositor

    Michael Davidson

    Article

    Gao et al Cell. 2012 Dec 7;151(6):1370-85. doi: 10

    Insert

    H2B (H2BC11 Human)
    

    Use

    Tags

    mEmerald

    Expression

    Mammalian

    Mutation

    D26G and V119I in H2B

    Promoter

    CMV

    Available since

    June 16, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits

38. pDECKO_TFRC_B

    Plasmid

    #72626

    Purpose

    Expresses two gRNAs targeting the TFRC promoter

    Depositor

    Roderic Guigo, Rory Johnson

    Article

    Aparicio-Prat et al BMC Genomics. 2015 Oct 23;16(1):846. doi

    Insert

    gRNAs toward TFRC
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6 (gRNA1) and H1 (gRNA2)

    Available since

    June 14, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pAAV Fos YFP MS2

    Plasmid

    #131775

    Purpose

    Fos upstream msfYFP 24xMS2 Fos downstream for CRISPR tagging

    Depositor

    Jared Toettcher

    Article

    Wilson et al Mol Cell. 2017 Sep 7;67(5):757-769.e5. d

    Insert

    msfYFP - 24x MS2 loops
    

    Use

    AAV and CRISPR

    Tags

    Expression

    Mutation

    Promoter

    n/a

    Available since

    Sept. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pGEX SHIP2-SH2

    Plasmid

    #46493

    Depositor

    Bruce Mayer

    Article

    Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

    Insert

    SHIP2
    

    Use

    Tags

    GST and PreScission

    Expression

    Bacterial

    Mutation

    contains amino acids 8-131

    Promoter

    Tac

    Available since

    July 10, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge