We narrowed to 9,009 results for: App

1. pCN-mBFP

   Plasmid

   #63847

   Purpose

   Encodes a NADPH dependent blue fluorescent protein expressed under a strong promoter and strong RBS without His-tagged.

   Depositor

   Howard Salis

   Article

   Ng et al Metab Eng. 2015 May;29:86-96. doi: 10.10

   Insert

   mBFP
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter and RBS was swapped. His-tag was removed…

   Promoter

   Available since

   April 3, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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2. T7-RelA(S536E)

   Plasmid

   #24156

   Depositor

   Warner Greene

   Article

   Chen et al Mol Cell Biol. 2005 Sep . 25(18):7966-75

   Insert

   RelA (RELA Human)
   

   Use

   Tags

   T7 gp10

   Expression

   Mammalian

   Mutation

   S536E

   Promoter

   Available since

   April 29, 2010

   Availability

   Academic Institutions and Nonprofits only

3. pMCSG53-DsbC

   Plasmid

   #186623

   Purpose

   E. coli expression vector containing N-terminal leaderless DsbC-6His-TEV fusion.

   Depositor

   Alexei Savchenko

   Article

   Venkatesan et al iScience. 2022 Sep 3;25(10):105054. doi:

   Type

   Empty backbone

   Use

   Tags

   DsbC-6His-TEV

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Sept. 19, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pBbdCas9S_Psyn-sgRNA500

   Plasmid

   #149640

   Purpose

   parental, all-in-one CRISPRi vector for B. burgdorferi, parental for gRNA cloning

   Depositor

   Christine Jacobs-Wagner

   Article

   Takacs et al Appl Environ Microbiol. 2020 Nov 30. pii

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   PflaB, PpQE30, Psyn

   Available since

   Oct. 26, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. T7-RelA(KR)

   Plasmid

   #23251

   Depositor

   Warner Greene

   Article

   Chen et al EMBO J. 2002 Dec 2. 21(23):6539-48.

   Insert

   NFkB RelA (RELA Human)
   

   Use

   Tags

   T7 gp10

   Expression

   Mammalian

   Mutation

   K218/221R, K310R

   Promoter

   Available since

   March 15, 2010

   Availability

   Academic Institutions and Nonprofits only

6. pEBTet_NADP-Snifit-mito

   Plasmid

   #113919

   Purpose

   Plasmid encoding the mitochondrial localization of the FRET semi-synthetic biosensor measuring the ratio NADPH/NADP+ in mammalian cells

   Depositor

   Kai Johnsson

   Article

   Sallin et al eLife. 2018 May 29;7. pii: 32638. doi: 1

   Insert

   NADP-Snifit
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV TetO2

   Available since

   Sept. 19, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pEXPqcxip-hSURF4-FLAG

   Plasmid

   #159139

   Purpose

   Hu SURF4 -flag mammalian expression Gateway vector.

   Depositor

   Martin Dorf

   Article

   Li et al Immunity. 2011 Sep 23;35(3):426-40. Epub

   Insert

   surfeit 4 (SURF4 Human)
   

   Use

   Retroviral

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV (cytomegalovirus) enhancer and the MSV (mouse…

   Available since

   Jan. 14, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. MAC-tag-C

   Plasmid

   #108077

   Purpose

   Gateway compatible destination vectors containing StrepIII/HA/BioID

   Depositor

   Markku Varjosalo

   Article

   Liu et al Nat Commun. 2018 Mar 22;9(1):1188. doi:

   Insert

   CCDB (ccdB(letD) )
   

   Use

   Tags

   StrepIII-HA-BirA*

   Expression

   Bacterial and Mammalian

   Mutation

   Promoter

   Available since

   April 26, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pEGFP-C1X-Nup88

   Plasmid

   #87338

   Purpose

   To express human Nup88 in mammalian cells

   Depositor

   Lei Lu

   Article

   Tie et al Biochem Biophys Res Commun. 2016 Sep 30;

   Insert

   NUP88 (NUP88 Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   May 4, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. pRS156

    Plasmid

    #182707

    Purpose

    CRISPRa plasmid for Candida albicans (dCas9-VPR and sgRNA cloning site)

    Depositor

    Rebecca Shapiro

    Article

    Gervais et al G3 (Bethesda). 2022 Dec 1:jkac301. doi:

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Nov. 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. mCherry-FKBP-PI4KB

    Plasmid

    #139310

    Purpose

    Recruitable PI4KB

    Depositor

    Gerry Hammond

    Article

    Zewe et al J Cell Biol. 2020 Mar 2;219(3). pii: 133

    Insert

    mCherry: FKBP1A(3-108): GGSA]4GG:PI4KB (PI4KB Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCAG-Roxed-Cre

    Plasmid

    #51273

    Purpose

    Component of the Co-Driver binary site-specific recombinase system, generates functional rox60-71Cre upon removal of an internal STOP cassette by Dre

    Depositor

    Pawel Pelczar

    Article

    Hermann et al Nucleic Acids Res. 2014 Jan 9.

    Insert

    Roxed-Cre
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    June 2, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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13. pLVU/RED

    Plasmid

    #24178

    Depositor

    Lars Ittner

    Article

    Krupka et al Plasmid. 2010 May;63(3):155-60. doi: 10.

    Insert

    Chloramphenicol resistance gene (CmR) - ccdB gene
    

    Use

    Lentiviral; Gateway

    Tags

    DsRED

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 23, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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14. pBBRBB-eGFP

    Plasmid

    #32549

    Depositor

    Claudia Schmidt-dannert

    Article

    Vick et al Appl Microbiol Biotechnol. 2011 Dec;92(6

    Insert

    eGFP (eGFP )
    

    Use

    Synthetic Biology

    Tags

    His

    Expression

    Bacterial

    Mutation

    Promoter

    Consitutive lac

    Available since

    Nov. 8, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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15. p3E-FRT-KanR-FRT pA

    Plasmid

    #82601

    Purpose

    3' entry vector containing FRT-flanked kanamycin resistance cassette (KanR) with pA for FLP-induced kanamycin resistance, drug selection

    Depositor

    Kryn Stankunas

    Article

    Fowler et al PLoS One. 2016 Aug 8;11(8):e0159277. doi

    Insert

    FRT-Kan-FRT
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    None

    Available since

    Dec. 21, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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16. pEBTet_NAD-Snifit-mito

    Plasmid

    #113916

    Purpose

    Plasmid encoding the mitochondrial localization of the FRET semi-synthetic biosensor measuring NAD+ concentration in mammalian cells

    Depositor

    Kai Johnsson

    Article

    Sallin et al eLife. 2018 May 29;7. pii: 32638. doi: 1

    Insert

    NAD-Snifit
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV TetO2

    Available since

    Sept. 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. pST-2K

    Plasmid

    #44564

    Purpose

    for ectopic constitutive expression of two mycobacterial proteins in one vector

    Depositor

    Vinay Nandicoori

    Article

    Parikh et al Appl Environ Microbiol. 2013 Mar;79(5):1

    Type

    Empty backbone

    Use

    E.coli-mycobacteria shuttle vector

    Tags

    Flag tag and His tag

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Feb. 18, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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18. pFREE_Cm

    Plasmid

    #92052

    Purpose

    Allows inducible expression of gRNAs and Cas9 for plasmid curing.

    Depositor

    Morten Norholm

    Article

    Lauritsen et al Microb Cell Fact. 2017 Aug 2;16(1):135.

    Inserts

    Cas9
    gRNA array
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    pRhamBAD and ptet

    Available since

    Aug. 3, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. PB_TRE_sg61-PDGFR_EF1a_eGFP

    Plasmid

    #186310

    Purpose

    Plasmid for inducible mammalian cell expression of the sg61 helixCAM and constitutive expression of an identifying fluorescent protein (eGFP). Flanked with PiggyBac sites for easy line construction

    Depositor

    George Church

    Article

    Chao et al Cell. 2022 Aug 23. pii: S0092-8674(22)01

    Inserts

    sg61-PDGFR
    eGFP
    

    Use

    Tags

    His, myc

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 10, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. AAV-FLEX-rev-ChR2(H134R)-mCherry

    Plasmid

    #18916

    Purpose

    Cre-dependent viral expression of ChR2(H134R)-mCherry for neuronal excitation

    Depositor

    Scott Sternson

    Article

    Atasoy et al J Neurosci. 2008 Jul 9. 28(28):7025-30.

    Insert

    Channelrhodopsin 2-mCherry
    

    Use

    AAV and Cre/Lox; Adeno-associated virus

    Tags

    mCherry

    Expression

    Mutation

    H134R

    Promoter

    CAG

    Available since

    Jan. 29, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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