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pcDNA3.1-NLS-iPASnFR-H-mScarlet3-3xFLAG

Plasmid

#225245

Purpose

Mammalian expression of iPASnFR-H in the nucleus

Depositor

Loren Looger

Article

Tamura et al bioRxiv 2024.08.21.609037

Insert

iPASnFR-H

Tags

3xFLAG and mScarlet3

Expression

Mammalian

Available Since

Sept. 16, 2024

Availability

Academic Institutions and Nonprofits only

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LifeAct-mGarnet

Plasmid

#104307

Purpose

codes for a fusion of fluorescent protein mGarnet and lifeact that targets F-actin in live cells

Depositor

Ulrich Nienhaus

Article

Hense et al Sci Rep. 2015 Dec 9;5:18006. doi: 10.103

Insert

LifeAct-mGarnet

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 24, 2018

Availability

Academic Institutions and Nonprofits only

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pSWAP_Entry_H2_beta_CM

Plasmid

#184870

Purpose

pSwap plasmid part with H2_beta homology arms and chloramphenicol cassette

Depositor

Patrick Sobetzko

Article

Teufel et al Nat Commun. 2022 Jun 14;13(1):3430. doi:

Insert

lacZalpha,ccdB

Expression

Bacterial

Available Since

March 2, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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pMPRA1-CMV-Clover

Plasmid

#53538

Purpose

Fluorescent reporter control for pMPRA1

Depositor

Tarjei Mikkelsen

Article

Melnikov et al J Vis Exp. 2014 Aug 17;(90). doi: 10.379

Insert

Clover GFP

Expression

Mammalian

Promoter

CMV IE

Available Since

May 20, 2014

Availability

Academic Institutions and Nonprofits only

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pSWAP_Entry_H1_alpha

Plasmid

#184865

Purpose

pSwap plasmid part with H1_alpha homology arms

Depositor

Patrick Sobetzko

Article

Teufel et al Nat Commun. 2022 Jun 14;13(1):3430. doi:

Insert

lacZalpha,ccdB

Expression

Bacterial

Available Since

Jan. 3, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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pMPRA2-CMV-Clover

Plasmid

#53539

Purpose

Fluorescent reporter control for pMPRA2

Depositor

Tarjei Mikkelsen

Article

Melnikov et al J Vis Exp. 2014 Aug 17;(90). doi: 10.379

Insert

Clover GFP

Expression

Mammalian

Promoter

CMV IE

Available Since

May 20, 2014

Availability

Academic Institutions and Nonprofits only

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pMPRA3-CMV-Clover

Plasmid

#53540

Purpose

Fluorescent reporter control for pMPRA3

Depositor

Tarjei Mikkelsen

Article

Melnikov et al J Vis Exp. 2014 Aug 17;(90). doi: 10.379

Insert

Clover GFP

Expression

Mammalian

Promoter

CMV IE

Available Since

May 20, 2014

Availability

Academic Institutions and Nonprofits only

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pTD103aiiA(Cm)

Plasmid

#48886

Purpose

Negative feedback plasmid from oscillator

Depositor

Jeff Hasty

Article

Prindle et al Nature. 2011 Dec 18;481(7379):39-44. doi

Insert

aiiA and LuxR

Use

Synthetic Biology

Tags

ssrA tag (AANDENYALAA)

Expression

Bacterial

Promoter

pLux

Available Since

Feb. 6, 2014

Availability

Academic Institutions and Nonprofits only

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pBHt2

Plasmid

#104175

Purpose

Empty backbone for Agrobacterium tumefaciens-mediated transformation of Fusarium oxysporum

Depositor

Seogchan Kang

Article

Mullins et al Phytopathology. 2001 Feb;91(2):173-80. d

Type

Empty backbone

Use

Fungal expression

Promoter

none

Available Since

Jan. 3, 2018

Availability

Academic Institutions and Nonprofits only

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pHR_EF1a-->myc-mtLaG2

Plasmid

#162224

Purpose

Lentiviral vector for constitutive expression of Myc GFP nanobody Lag2 in mammalian cells

Depositor

Wendell Lim

Article

Toda et al Science. 2020 Oct 16;370(6514):327-331.

Insert

anti-GFP nanobody LaG2

Use

Lentiviral

Expression

Mammalian

Available Since

June 28, 2022

Availability

Academic Institutions and Nonprofits only

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OmpX

Plasmid

#213136

Purpose

Expresses outer membrane protein X

Depositor

Sebastian Hiller

Article

Rath et al Biochim Biophys Acta Biomembr. 2020 Jan

Insert

OmpX (24-171) (ompX E.coli (K12))

Expression

Bacterial

Promoter

T7

Available Since

Feb. 7, 2024

Availability

Academic Institutions and Nonprofits only

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pAN1201

Plasmid

#74695

Purpose

Circuit backbone plasmid (LacZa insert)

Depositor

Christopher Voigt

Article

Nielsen et al Science. 2016 Apr 1;352(6281):aac7341. d

Type

Empty backbone

Expression

Bacterial

Available Since

June 6, 2016

Availability

Academic Institutions and Nonprofits only

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pHR_Ef1a-->flagLaM4_Notch_tTA

Plasmid

#162237

Purpose

Lentiviral vector for the expression of LaM 4 mCherry nanobody notch tTA: flagLaM4_Notch_tTA in mammalian cells

Depositor

Wendell Lim

Article

Toda et al Science. 2020 Oct 16;370(6514):327-331.

Insert

LaM4 mCherry nanobody Notch tTA

Use

Lentiviral

Expression

Mammalian

Available Since

Aug. 30, 2022

Availability

Academic Institutions and Nonprofits only

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pEB2-2ndVal mScarlet-I

Plasmid

#104028

Purpose

Plasmid encoding 2ndVal mScarlet-I

Depositor

Philippe Cluzel

Article

Balleza et al Nat Methods. 2018 Jan;15(1):47-51. doi:

Insert

2ndVal mScarlet-I

Use

Low copy

Expression

Bacterial

Mutation

mScarlet-I with valine inserted in 2nd position

Promoter

proC

Available Since

Dec. 27, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+)-OTI-TCRB_p2_TCRA

Plasmid

#131034

Purpose

Expresses OT-I TCRB linked to TCRA with the P2A self-cleaving peptide

Depositor

Jeff Hammerbacher

Article

Aksoy et al bioRxiv

Inserts

TCRB (Tcrb Mouse)
TCRA (Tcra Mouse)

Expression

Mammalian

Promoter

CMV/T7

Available Since

Oct. 8, 2019

Availability

Academic Institutions and Nonprofits only

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pEGFP-N1-VAPA(1-242)

Plasmid

#18874

Depositor

Axel Brunger

Article

Kaiser et al Structure. 2005 Jul . 13(7):1035-45.

Insert

VAP-A (Vapa Rat)

Tags

GFP

Expression

Mammalian

Available Since

Sept. 19, 2008

Availability

Academic Institutions and Nonprofits only

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pSWAP_Entry_INS

Plasmid

#184867

Purpose

pSwap plasmid part with insert fragment module

Depositor

Patrick Sobetzko

Article

Teufel et al Nat Commun. 2022 Jun 14;13(1):3430. doi:

Insert

lacZalpha,ccdB

Expression

Bacterial

Available Since

Jan. 3, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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pcDNA3-APX(W41F)-NES

Plasmid

#42608

Depositor

Alice Ting

Article

Rhee et al Science. 2013 Jan 31.

Insert

soybean APX W41F (dimeric) -NES

Tags

Flag

Expression

Mammalian

Mutation

W41F mutation

Promoter

CMV

Available Since

March 20, 2013

Availability

Academic Institutions and Nonprofits only

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pBN42

Plasmid

#86713

Purpose

C. elegans injection marker

Depositor

Peter Askjaer

Article

Morales-Martinez et al J Cell Sci. 2015 Mar 15;128(6):1090-6. d

Insert

Pmyo-3::GFP

Expression

Worm

Available Since

Feb. 7, 2017

Availability

Academic Institutions and Nonprofits only

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pEERM4 Cm

Plasmid

#64026

Purpose

Plasmid for integration into neutral site 2 of Synechocystis 6803

Depositor

Pia Lindberg

Article

Englund et al ACS Synth Biol. 2015 Jul 13.

Type

Empty backbone

Use

Synthetic Biology

Expression

Bacterial

Promoter

PnrsB

Available Since

May 29, 2015

Availability

Academic Institutions and Nonprofits only

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