We narrowed to 9,799 results for: otos

1. pTS100 pHAGE2 CMVtetO2 TagBFP-P2A-ALFA-22xGS-TEV-MCS

   Plasmid

   #199353

   Purpose

   Lentiviral transfer plasmid for doxycycline inducible expression of a N-terminally BFP-P2A-ALFA-TEV-tagged protein in mammalian cells.

   Depositor

   Rebecca Voorhees

   Article

   Stevens et al Nat Protoc. 2024 Jan;19(1):127-158. doi:

   Type

   Empty backbone

   Use

   Lentiviral

   Tags

   TagBFP-P2A-ALFA-TEV

   Expression

   Mammalian

   Mutation

   WT

   Promoter

   Available since

   May 23, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pDONR223-VPS35

   Plasmid

   #21689

   Depositor

   Lynda Chin

   Article

   Scott et al Nature. 2009 Jun 25. 459(7250):1085-90.

   Insert

   Vacuolar Protein Sorting 35 homolog (VPS35 Human)
   

   Use

   Gateway entry clone

   Tags

   Expression

   Mutation

   last nucleotide of stop codon removed for c-termi…

   Promoter

   Available since

   April 30, 2010

   Availability

   Academic Institutions and Nonprofits only

3. pKLV2-EF1a-Cas9GFP-W

   Plasmid

   #200100

   Purpose

   Lentiviral vector expressing Cas9 fused with GFP at the C-terminus

   Depositor

   Kosuke Yusa

   Article

   Aoki et al Blood. 2023 Nov 3:blood.2023020857. doi:

   Insert

   GFP
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Jan. 17, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCarCBADE_ProB(I69E)A

   Plasmid

   #117756

   Purpose

   ProB(I69E)A and codon optimized CarCBADE enzymes (in that order) in pBbaA5k backbone

   Depositor

   Greg Bokinsky

   Article

   Shomar et al Nat Chem Biol. 2018 Aug;14(8):794-800. d

   Insert

   Glutamate kinase (ProA), mutant pyrroline-5-carboxylate synthase (ProBI69E) from E. coli and codon optimised CarCBADE operon
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   PLac(UV5)

   Available since

   Dec. 5, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pME-lox-3xSTOP-lox (JDW 869)

   Plasmid

   #224521

   Purpose

   A Gateway compatible middle entry clone containing a loxP flanked SV40 polyA, bGH polyA, and SV40 pA stop cassette

   Depositor

   Joshua Wythe

   Article

   Gillespie et al bioRxiv 2024.07.13.603267

   Insert

   LoxP-3xStop-LoxP
   

   Use

   Cre/Lox

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Oct. 1, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pME-mScarlet-I-P2A-iCre (JDW 917)

   Plasmid

   #224516

   Purpose

   A Gateway-compatible middle entry clone containing mScarlet-I P2A-iCre (codon optimized)

   Depositor

   Joshua Wythe

   Article

   Gillespie et al bioRxiv 2024.07.13.603267

   Insert

   mScarlet-I-P2A-iCre
   

   Use

   Cre/Lox

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Sept. 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAAV-Syn-FLEX-ExSYTE

   Plasmid

   #216263

   Purpose

   Cre-dependent expressoin of ExSYTE (P2A) EGFP with 3'UTR of Rat Arc DTE under synapsin promoter

   Depositor

   Susumu Tomita

   Article

   Park et al Cell Rep. 2023 Aug 29;42(8):112826. doi:

   Insert

   ExSYTE P2A EGFP - Rat DTE
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   Available since

   May 8, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. 3xFLAG-dCas9/pMSCVneo

   Plasmid

   #134982

   Purpose

   Expresses 3xFLAG-dCas9 in mammalian cells for enChIP analysis to purify specific genomic regions of interest.

   Depositor

   Hodaka Fujii

   Article

   Yuno et al Biol Methods Protoc. 2021 Jul 9;6(1):bpa

   Insert

   3xFLAG-dCas9
   

   Use

   CRISPR and Retroviral

   Tags

   3xFLAG tag and NLS

   Expression

   Mammalian

   Mutation

   human codon-optimized, D10A + H840A

   Promoter

   LTR

   Available since

   April 22, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. CMV-GEX-GECO1

   Plasmid

   #32443

   Purpose

   Mammalian expression of green excitation ratiometric genetically encoded Ca2+ indicator for optical imaging

   Depositor

   Robert Campbell

   Article

   Zhao et al Science. 2011 Sep 30;333(6051):1888-91.

   Insert

   GEX-GECO1.0
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   GCaMP3 K69E/K119I/L173Q/T223S/F315L/F368L/L372Q/K…

   Promoter

   CMV

   Available since

   Sept. 29, 2011

   Availability

   Academic Institutions and Nonprofits only

10. pHR-CMV-TetO2_3C-mVenus-Twin-Strep

    Plasmid

    #113891

    Purpose

    Mammalian (inducible) protein expression, C-terminal 3C-mVenus-Twin-Strep fusion

    Depositor

    A. Radu Aricescu

    Article

    Elegheert et al Nat Protoc. 2018 Nov 19. pii: 10.1038/s4

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    3C-mVenus-Twin-Strep

    Expression

    Mutation

    Promoter

    CMV-MIE-TetO2

    Available since

    Aug. 27, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCXLE-Fbx15-cont2

    Plasmid

    #27081

    Depositor

    Shinya Yamanaka

    Article

    Okita et al Nat Methods. 2011 May;8(5):409-12

    Insert

    FBXO15 (FBXO15 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 12, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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12. pDF0250 DisCas7-11 full locus with Golden gate acceptor site for spacer

    Plasmid

    #172501

    Purpose

    Encodes the DisCas7-11 full locus along with a DR and golden gate site for cloning spacers

    Depositor

    Omar Abudayyeh, Jonathan Gootenberg

    Article

    Ozcan et al Nature. 2021 Sep 6. pii: 10.1038/s41586-

    Insert

    DisCas7-11 full locus with Golden gate acceptor site for spacer
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. EYFP-Mito-7

    Plasmid

    #56596

    Purpose

    Localization: Mitochondria, Excitation: 513, Emission: 527

    Depositor

    Michael Davidson

    Article

    Rizzo et al Cold Spring Harb Protoc. 2009 Dec;2009(1

    Insert

    Mito (COX8A Human)
    

    Use

    Tags

    EYFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 29, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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14. pcDNA3.1 SIRT6_H133Y

    Plasmid

    #53149

    Purpose

    expression vector for catalyitc inactive human Sirtuin6

    Depositor

    Katrin Chua

    Article

    Michishita et al Nature. 2008 Mar 27;452(7186):492-6. doi

    Insert

    Sirt6 (SIRT6 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    H133Y, catalytic inactive

    Promoter

    CMV

    Available since

    May 16, 2014

    Availability

    Academic Institutions and Nonprofits only

15. NLS-CanlonicSF

    Plasmid

    #188390

    Purpose

    To explore lactate dynamics in intact systems

    Depositor

    Alejandro San Martín

    Article

    Aburto et al ACS Sens. 2022 Oct 28. doi: 10.1021/acss

    Insert

    NLS-CanlonicSF
    

    Use

    Tags

    NLS and myc

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pB[vasa-Cas9 PUb-ZsGreen]

    Plasmid

    #169012

    Purpose

    For germline transformation. Expresses Cas9 in germ cells and ZsGreen marker in all cells.

    Depositor

    Max Scott

    Article

    Kandul et al CRISPR J. 2021 Oct;4(5):739-751. doi: 10

    Insert

    Cas9
    

    Use

    Piggybac

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Drosophila melanogaster vasa

    Available since

    April 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pME-Lifeact-EGFP-3xHA (JDW 1322)

    Plasmid

    #224494

    Purpose

    Gateway compatible middle entry clone containing Lifeact-EGFP (EGFP F-actin reporter)

    Depositor

    Joshua Wythe

    Article

    Gillespie et al bioRxiv 2024.07.13.603267

    Insert

    Liefact-EGFP-3xHA
    

    Use

    Gateway cloning

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. AAV-mCREB

    Plasmid

    #68551

    Purpose

    AAV expression of mCREB

    Depositor

    Eric Nestler

    Article

    Berger et al Biol Psychiatry. 2011 Feb 1;69(3):288-94

    Insert

    mCREB
    

    Use

    AAV

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    S133A

    Promoter

    CMV

    Available since

    Sept. 2, 2015

    Availability

    Academic Institutions and Nonprofits only

19. AAV-FLEX-FLIM-AKART391A

    Plasmid

    #60446

    Purpose

    Expresses the non-phosphorylatable point mutant (T391A) of FLIM-AKAR in a Cre dependent manner in an AAV backbone

    Depositor

    Bernardo Sabatini

    Article

    Chen et al Front Pharmacol. 2014 Apr 2;5:56. doi: 1

    Insert

    FLIM-AKART391A
    

    Use

    AAV and Cre/Lox

    Tags

    Expression

    Mammalian

    Mutation

    changed Threonine 391 to Ananine

    Promoter

    CAG

    Available since

    Oct. 22, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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20. pPPEDs

    Plasmid

    #162469

    Purpose

    protoplast test vector of pPPED

    Depositor

    Adam Bogdanove

    Article

    Wang et al Front Genome Ed. 2021 Mar 4;3:617553. do

    Inserts

    Cas9H840A
    attR gateway destination cassette
    

    Use

    Prime editing destination vector for transient ex…

    Tags

    MLV reverse transcriptase

    Expression

    Plant

    Mutation

    Change histidine 840 to Alanine

    Promoter

    Available since

    Dec. 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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21. MSCV-mouse p19ARF-IRES-GFP

    Plasmid

    #107147

    Purpose

    Mammalian expression of mouse Arf

    Depositor

    Martine Roussel

    Article

    Kuo et al Cancer Res. 2003 Mar 1;63(5):1046-53.

    Insert

    Arf [alternative reading frame] (Cdkn2a Mouse)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 3, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. CFP-CD82

    Plasmid

    #1818

    Depositor

    Walther Mothes

    Article

    Sherer et al Traffic 2003 Nov;4(11):785-801.

    Insert

    CD82 (CD82 Human)
    

    Use

    Tags

    CFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 4, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. 4xnrUAS:MLS-KillerRed in pTol2pA2-acrys-EGFP-

    Plasmid

    #115516

    Purpose

    Plasmid driving expression of membrane bound KillerRed fluorophore under UAS response element, with a green lens reporter

    Depositor

    Marco Morsch

    Article

    Formella et al Redox Biol. 2018 Aug 23;19:226-234. doi:

    Insert

    MLS-KillerRed
    

    Use

    Gateway cloning

    Tags

    MLS

    Expression

    Mutation

    Promoter

    UAS

    Available since

    Oct. 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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24. pHR-CMV-TetO2_HA-BirA

    Plasmid

    #113896

    Purpose

    Mammalian (inducible) expression of HA-tagged cytosolic E. coli biotin ligase

    Depositor

    A. Radu Aricescu

    Article

    Elegheert et al Nat Protoc. 2018 Nov 19. pii: 10.1038/s4

    Insert

    E. coli biotin ligase
    

    Use

    Lentiviral

    Tags

    HA

    Expression

    Mutation

    Promoter

    CMV-MIE-TetO2

    Available since

    Aug. 28, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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25. pCAG-Bmpr2ΔKinase

    Plasmid

    #163404

    Purpose

    Expresses BMPR-2 lacking the kinase domain in mammalian cells

    Depositor

    Takeshi Imai

    Article

    Aihara et al Cell Rep. 2021 Jun 22;35(12):109276. doi

    Insert

    Bmpr2 (Bmpr2 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    The kinase domain (202-500 aa) is deleted

    Promoter

    Available since

    Aug. 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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26. pMXSIP-FLAG-ATPIF1

    Plasmid

    #204703

    Purpose

    expresses FLAG-ATPIF1 in mammalian cells

    Depositor

    William Kaelin

    Article

    Wyant et al Science. 2022 Aug 5;377(6606):621-629. d

    Insert

    ATP synthase inhibitory factor subunit 1 (ATP5IF1 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pMK312 (TOP2A CRISPR)

    Plasmid

    #140654

    Purpose

    TOP2A tagging CRISPR

    Depositor

    Masato Kanemaki

    Article

    Yesbolatova et al Nat Commun. 2020 Nov 11;11(1):5701. doi:

    Insert

    TOP2A (TOP2A Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 12, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pW193-lenti-sasgRNA-lacZ-pEF1s-NLS-mNeonGreen-P2A-BlastR

    Plasmid

    #170814

    Purpose

    Lentiviral vector to co-express a lacZ control sasgRNA with NLS-mNeonGreen

    Depositor

    Kenneth Yamada

    Article

    Wang et al Cell. 2021 Jul 8;184(14):3702-3716.e30.

    Insert

    NLS-mNeonGreen-P2A-BlastR
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    NA

    Promoter

    Available since

    June 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pmCherry-MYRIP

    Plasmid

    #89586

    Purpose

    Expression plasmid for a positive control prey protein in NanoSPD 2.0 assays.

    Depositor

    Thomas Friedman

    Article

    Bird et al Mol Biol Cell. 2016 Dec 8. pii: mbc.E16-

    Insert

    MYRIP (Myrip Mouse)
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 25, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pTS114 pHAGE2 CMVtetO2 MCS-TEV-SUMOEu-22xGS-Alfa-P2A-TagBFP

    Plasmid

    #199367

    Purpose

    Lentiviral transfer plasmid for doxycycline inducible expression of a C-terminally TEV-SUMOEu-ALFA-P2A-BFP-tagged protein in mammalian cells.

    Depositor

    Rebecca Voorhees

    Article

    Stevens et al Nat Protoc. 2024 Jan;19(1):127-158. doi:

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    TEV-SUMOEu-ALFA-P2A-TagBFP

    Expression

    Mammalian

    Mutation

    WT

    Promoter

    Available since

    April 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge