Addgene

We narrowed to 14,622 results for: NOL

Showing: 4951 - 4980 of 14622 results

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pcDNA3 Mito-Ras-LOCKR-PL Key

Plasmid

#216507

Purpose

Ras activity dependent proximity labeler, Key only, mitochondria localized

Depositor

David Baker

Article

Zhang et al Nat Biotechnol. 2024 Jan 25. doi: 10.103

Insert

Mito-Ras-LOCKR-PL

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 2, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNAintron-LASV-GPC-c3xFLAG

Plasmid

#215689

Purpose

Produces the Lassa virus glycoprotein with a c-terminal 3xFLAG tag

Depositor

Melinda Brindley

Article

Acciani et al J Virol. 2017 Aug 24;91(18):e00574-17. d

Insert

LASV GP-FLAG

Use

Tags

3xFLAG

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 25, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pcDNA3.1-TST-EGFP-GPIDAF

Plasmid

#213712

Purpose

For overexpression of a gene, accompanied by the expression of the affinity sorting tag TST-EGFP-GPIDAF driven by an SV40 promoter.

Depositor

Qilai Huang

Article

Yang et al Front Bioeng Biotechnol. 2022 Jun 16;10:

Insert

EGFP

Use

Tags

Twin-strep-tag and V5-tag

Expression

Mammalian

Mutation

Promoter

SV40

Available since

Feb. 16, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-TST-EGFP-GPIBY55

Plasmid

#213711

Purpose

For overexpression of a gene, accompanied by the expression of the affinity sorting tag TST-EGFP-GPIBY55 driven by an SV40 promoter.

Depositor

Qilai Huang

Article

Yang et al Front Bioeng Biotechnol. 2022 Jun 16;10:

Insert

EGFP

Use

Tags

Twin-strep-tag and V5-tag

Expression

Mammalian

Mutation

Promoter

SV40

Available since

Feb. 16, 2024

Availability

Academic Institutions and Nonprofits only

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ABE-RA4.1

Plasmid

#208306

Purpose

Expresses ABE-RA4.1 in mammalian cells

Depositor

Weixin Tang

Article

Xiao et al Nat Biotechnol. 2024 Jan 2. doi: 10.1038

Insert

TadA-RA4.1-SpCas9 D10A

Use

Tags

Expression

Mammalian

Mutation

P48A, R51H, I76F, A106V, D108G, K110R, T111H, D11…

Promoter

Available since

Jan. 2, 2024

Availability

Academic Institutions and Nonprofits only

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ABE-RA4.2

Plasmid

#208307

Purpose

Expresses ABE-RA4.2 in mammalian cells

Depositor

Weixin Tang

Article

Xiao et al Nat Biotechnol. 2024 Jan 2. doi: 10.1038

Insert

TadA-RA4.2-SpCas9 D10A

Use

Tags

Expression

Mammalian

Mutation

P48A, R51H, I76F, A106V, D108G, K110R, T111H, D11…

Promoter

Available since

Jan. 2, 2024

Availability

Academic Institutions and Nonprofits only

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ABE-RA4.3

Plasmid

#208308

Purpose

Expresses ABE-RA4.3 in mammalian cells

Depositor

Weixin Tang

Article

Xiao et al Nat Biotechnol. 2024 Jan 2. doi: 10.1038

Insert

TadA-RA4.3-SpCas9 D10A

Use

Tags

Expression

Mammalian

Mutation

P48A, R51H, I76F, A106V, D108G, K110R, T111H, D11…

Promoter

Available since

Jan. 2, 2024

Availability

Academic Institutions and Nonprofits only

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ABE-RA4.4

Plasmid

#208309

Purpose

Expresses ABE-RA4.4in mammalian cells

Depositor

Weixin Tang

Article

Xiao et al Nat Biotechnol. 2024 Jan 2. doi: 10.1038

Insert

TadA-RA4.4-SpCas9 D10A

Use

Tags

Expression

Mammalian

Mutation

P48A, R51H, I76F, A106V, D108G, K110R, T111H, D11…

Promoter

Available since

Jan. 2, 2024

Availability

Academic Institutions and Nonprofits only

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ABE-RA4.5

Plasmid

#208310

Purpose

Expresses ABE-RA4.5 in mammalian cells

Depositor

Weixin Tang

Article

Xiao et al Nat Biotechnol. 2024 Jan 2. doi: 10.1038

Insert

TadA-RA4.5-SpCas9 D10A

Use

Tags

Expression

Mammalian

Mutation

P48A, R51H, I76F, A106V, D108G, K110R, T111H, D11…

Promoter

Available since

Jan. 2, 2024

Availability

Academic Institutions and Nonprofits only

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ABE-RA4.6

Plasmid

#208311

Purpose

Expresses ABE-RA4.6 in mammalian cells

Depositor

Weixin Tang

Article

Xiao et al Nat Biotechnol. 2024 Jan 2. doi: 10.1038

Insert

TadA-RA4.6-SpCas9 D10A

Use

Tags

Expression

Mammalian

Mutation

P48A, R51H, I76F, A106V, D108G, K110R, T111H, D11…

Promoter

Available since

Jan. 2, 2024

Availability

Academic Institutions and Nonprofits only

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ABE-RA5.0

Plasmid

#208312

Purpose

Expresses ABE-RA5.0 in mammalian cells

Depositor

Weixin Tang

Article

Xiao et al Nat Biotechnol. 2024 Jan 2. doi: 10.1038

Insert

TadA-RA5.0-SpCas9 D10A

Use

Tags

Expression

Mammalian

Mutation

W23R, H36L, R47K, P48A, R51L, I76F, V82S, A106V, …

Promoter

Available since

Jan. 2, 2024

Availability

Academic Institutions and Nonprofits only

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ABE-RA5.1

Plasmid

#208313

Purpose

Expresses ABE-RA5.1 in mammalian cells

Depositor

Weixin Tang

Article

Xiao et al Nat Biotechnol. 2024 Jan 2. doi: 10.1038

Insert

TadA-RA5.1-SpCas9 D10A

Use

Tags

Expression

Mammalian

Mutation

W23R, H36L, R47K, P48A, R51L, I76F, V82S, A106V, …

Promoter

Available since

Jan. 2, 2024

Availability

Academic Institutions and Nonprofits only

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ABE-RA5.3

Plasmid

#208314

Purpose

Expresses ABE-RA5.3 in mammalian cells

Depositor

Weixin Tang

Article

Xiao et al Nat Biotechnol. 2024 Jan 2. doi: 10.1038

Insert

TadA-RA5.3-SpCas9 D10A

Use

Tags

Expression

Mammalian

Mutation

W23R, H36L, R47K, P48A, R51L, I76F, V82S, A106V, …

Promoter

Available since

Jan. 2, 2024

Availability

Academic Institutions and Nonprofits only

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ABE-RA5.4

Plasmid

#208315

Purpose

Expresses ABE-RA5.4 in mammalian cells

Depositor

Weixin Tang

Article

Xiao et al Nat Biotechnol. 2024 Jan 2. doi: 10.1038

Insert

TadA-RA5.4-SpCas9 D10A

Use

Tags

Expression

Mammalian

Mutation

W23R, R47K, P48A, R51L, I76Y, V82S, A106V, D108G,…

Promoter

Available since

Jan. 2, 2024

Availability

Academic Institutions and Nonprofits only

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ABE-RA5.6

Plasmid

#208317

Purpose

Expresses ABE-RA5.6 in mammalian cells

Depositor

Weixin Tang

Article

Xiao et al Nat Biotechnol. 2024 Jan 2. doi: 10.1038

Insert

TadA-RA5.6-SpCas9 D10A

Use

Tags

Expression

Mammalian

Mutation

W23R, H36L, R47K, P48A, R51L, I76Y, V82S, A106V, …

Promoter

Available since

Jan. 2, 2024

Availability

Academic Institutions and Nonprofits only

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pDN0520 (pDEST, TRP1)

Plasmid

#210481

Purpose

pDEST vector with TRP1 marker for gene expression in S. cerevisiae.

Depositor

Christian Landry

Article

Evans-Yamamoto et al Mol Biol Evol. 2023 Nov 18:msad246. doi:

Type

Empty backbone

Use

Destination vector for gateway cloning

Tags

Expression

Mutation

Promoter

ADH1

Available since

Jan. 2, 2024

Availability

Academic Institutions and Nonprofits only

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LLP765_L2pV3_ANIMPLYB_B3RT_FRT_OCS_Rluc_1IF_lacZ

Plasmid

#192409

Purpose

To act as a 1-input state of the A NIMPLY B gate.

Depositor

Ryan Lister

Article

Lloyd et al Nat Biotechnol. 2022 Jul 4. pii: 10.1038

Insert

B3RT-Act2-B3RT::FRT-OCS-FRT::Rluc

Use

Synthetic Biology

Tags

PEST

Expression

Plant

Mutation

Promoter

Available since

Sept. 8, 2023

Availability

Academic Institutions and Nonprofits only

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pQE-80L 2xHis-Rab29

Plasmid

#186021

Purpose

2xHis-Rab29

Depositor

Suzanne Pfeffer

Article

Vides et al Elife. 2022 Sep 23;11:e79771. doi: 10.75

Insert

2xHis-Rab29 (RAB29 Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 1, 2023

Availability

Academic Institutions and Nonprofits only

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NK809

Plasmid

#196406

Purpose

Strong expression of firefly and nanoluc luciferases codon-optimized for S. rosetta

Depositor

Nicole King

Article

Coyle et al Curr Biol. 2023 Aug 2:S0960-9822(23)0092

Insert

Sro5EFL-nanoluc-Sro3EFL/Sro5actin-firefly-Sro3actin

Use

Luciferase

Tags

Expression

Mutation

N/A (synthetic)

Promoter

Available since

Aug. 2, 2023

Availability

Academic Institutions and Nonprofits only

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NK810

Plasmid

#196407

Purpose

Nanoluc luciferase under S. rosetta SPAG6 promoter with internal actin-firefly control

Depositor

Nicole King

Article

Coyle et al Curr Biol. 2023 Aug 2:S0960-9822(23)0092

Insert

Sro5spag6-nanoluc-Sro3EFL/Sro5actin-firefly-Sro3actin

Use

Luciferase

Tags

Expression

Mutation

N/A (synthetic)

Promoter

Available since

Aug. 2, 2023

Availability

Academic Institutions and Nonprofits only

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NK811

Plasmid

#196408

Purpose

Nanoluc luciferase under S. rosetta SPAG6 promoter with mutated RFX binding site with internal actin-firefly control

Depositor

Nicole King

Article

Coyle et al Curr Biol. 2023 Aug 2:S0960-9822(23)0092

Insert

Sro5spag6_mut-nanoluc-Sro3EFL/Sro5actin-firefly-Sro3actin

Use

Luciferase

Tags

Expression

Mutation

N/A (synthetic)

Promoter

Available since

Aug. 2, 2023

Availability

Academic Institutions and Nonprofits only

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pIS001

Plasmid

#194012

Purpose

This plasmid is designed for use in a procedure to synthesize single-stranded DNA containing only the insert.

Depositor

Timothy Whitehead

Article

Strawn et al Biotechnol Bioeng. 2023 Oct;120(10):3057

Insert

ROSA-TRE-C16V5-G-pA

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 14, 2023

Availability

Academic Institutions and Nonprofits only

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LV TRE-GFP-TMD-DLL1-Nbox-mutant

Plasmid

#194937

Purpose

LV construct encoding a mutated GFP-TMD-DLL1 ligand containing an ablated Nbox site under dox-inducible TRE promoter and containing a PGK-rtTA-M2-IRES-Puro regulator/resistance marker cassette.

Depositor

John Ngo

Article

Sloas et al Nat Biotechnol. 2023 Jan 16. doi: 10.103

Insert

eGFP-(PDGFR)TMD-rDLL1 ICD-Nbox-AlaX3-mutant

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

March 3, 2023

Availability

Academic Institutions and Nonprofits only

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LLP744_L2pV1_OR_B3RT_FRT_Rluc_1IF_lacZ

Plasmid

#192388

Purpose

To act as a 1-input state of the OR gate.

Depositor

Ryan Lister

Article

Lloyd et al Nat Biotechnol. 2022 Jul 4. pii: 10.1038

Insert

Act2::FRT-B3RT-OCS-FRT-B3RT::Rluc

Use

Synthetic Biology

Tags

PEST

Expression

Plant

Mutation

Promoter

Available since

Feb. 1, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pIS003

Plasmid

#194014

Purpose

This plasmid is designed for use in a procedure to synthesize single-stranded DNA containing only the insert.

Depositor

Timothy Whitehead

Article

Strawn et al Biotechnol Bioeng. 2023 Oct;120(10):3057

Insert

C16 Ex4 XTR f1

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Jan. 31, 2023

Availability

Academic Institutions and Nonprofits only

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LLP761_L2pV1_OR-OCS_s35S-Cre

Plasmid

#192420

Purpose

To act as a 1-input state of the OR gate.

Depositor

Ryan Lister

Article

Lloyd et al Nat Biotechnol. 2022 Jul 4. pii: 10.1038

Insert

Act2::FRT-loxP-OCS-FRT-loxP::Rluc

Use

Synthetic Biology

Tags

PEST

Expression

Plant

Mutation

Promoter

Available since

Dec. 2, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
LLP673_L2_pV01_1I_VloxP-OCS-Rluc_TCTP_VCre_lacZ

Plasmid

#192425

Purpose

To act as the on state for testing of the alt VCre recombinase.

Depositor

Ryan Lister

Article

Lloyd et al Nat Biotechnol. 2022 Jul 4. pii: 10.1038

Insert

Act2::VloxP-OCS-VloxP::Rluc

Use

Synthetic Biology

Tags

PEST

Expression

Plant

Mutation

Promoter

Available since

Nov. 29, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
LLP749_L2pV1_AND_B3RT_FRT_Rluc_1IF_lacZ

Plasmid

#192393

Purpose

To act as a 1-input state of the AND gate.

Depositor

Ryan Lister

Article

Lloyd et al Nat Biotechnol. 2022 Jul 4. pii: 10.1038

Insert

Act2::FRT-OCS-FRT::B3RT-OCS-B3RT::Rluc

Use

Synthetic Biology

Tags

PEST

Expression

Plant

Mutation

Promoter

Available since

Nov. 29, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
LLP748_L2pV1_AND_B3RT_FRT_Rluc_0I_lacZ

Plasmid

#192392

Purpose

To act as the 0-input state of the AND gate.

Depositor

Ryan Lister

Article

Lloyd et al Nat Biotechnol. 2022 Jul 4. pii: 10.1038

Insert

Act2::FRT-OCS-FRT::B3RT-OCS-B3RT::Rluc

Use

Synthetic Biology

Tags

PEST

Expression

Plant

Mutation

Promoter

Available since

Nov. 29, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
LLP671_L2_pV01_1I_SloxM1-OCS-Rluc_TCTP_SCre_lacZ

Plasmid

#192423

Purpose

To act as the on state for testing of the alt SCre recombinase.

Depositor

Ryan Lister

Article

Lloyd et al Nat Biotechnol. 2022 Jul 4. pii: 10.1038

Insert

Act2::SloxM1-OCS-SloxM1::Rluc

Use

Synthetic Biology

Tags

PEST

Expression

Plant

Mutation

Promoter

Available since

Nov. 29, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge