We narrowed to 15,891 results for: NOL

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Tet-O-FUW-Nkx6.1

Plasmid

#45846

Depositor

Marius Wernig

Article

Yang et al Nat Biotechnol. 2013 May;31(5):434-9. do

Insert

Nkx6.1 (Nkx6-1 Mouse)

Use

Lentiviral

Expression

Mammalian

Promoter

tet-ON

Available Since

July 22, 2013

Availability

Academic Institutions and Nonprofits only

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pCellFree_G03 TCF12

Plasmid

#67113

Purpose

Standard for cell-free expression benchmarking.

Depositor

Kirill Alexandrov

Article

Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

Insert

TCF12 (TCF12 Human)

Use

Cell-free expression

Tags

EGFP and His

Promoter

T7

Available Since

Nov. 6, 2015

Availability

Academic Institutions and Nonprofits only
pBLO1806_Cas9_2xNLS_human

Plasmid

#74493

Purpose

Human codon optimized plasmid to express Cas9 t2a mCherry w/2xNLS and a sgRNA

Depositor

David Savage

Article

Oakes et al Nat Biotechnol. 2016 May 2. doi: 10.1038

Insert

Cas9

Tags

t2a mCherry

Expression

Mammalian

Available Since

May 20, 2016

Availability

Academic Institutions and Nonprofits only

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3xHA-BioID_pAS28

Plasmid

#118223

Purpose

expresses 3xHA-tagged BioID in C. elegans intestine

Depositor

Jessica Feldman

Article

Branon et al Nat Biotechnol. 2018 Aug 20. pii: nbt.42

Insert

BioID (BirA mutant)

Tags

3x HA tag

Expression

Worm

Mutation

R118G

Promoter

ges-1p

Available Since

Jan. 14, 2019

Availability

Academic Institutions and Nonprofits only

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apt-CBE-3'end

Plasmid

#219939

Purpose

Mammalian expression of apt-CBE-3'end protein (MCP_32aa-linker_evoAPOBEC1) with P2A-EGFP marker

Depositor

Alexis Komor

Article

Cowan et al Nat Biotechnol. 2024 May 21. doi: 10.103

Insert

MCP_32-aa_evo-rA1_2xUGI

Use

CRISPR

Expression

Mammalian

Promoter

CMV

Available Since

June 5, 2024

Availability

Academic Institutions and Nonprofits only

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apt-ABE8e

Plasmid

#219937

Purpose

Mammalian expression of apt-ABE8e protein (Com_93aa-linker_TadA8e) with P2A-EGFP marker

Depositor

Alexis Komor

Article

Cowan et al Nat Biotechnol. 2024 May 21. doi: 10.103

Insert

Com_93-aa_TadA8e

Use

CRISPR

Expression

Mammalian

Promoter

CMV

Available Since

June 5, 2024

Availability

Academic Institutions and Nonprofits only

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pMR-A-6

Plasmid

#138520

Purpose

P3d

Depositor

David Liu

Article

Richter et al Nat Biotechnol. 2020 Jul;38(7):883-891.

Insert

T7-RNAP

Use

Synthetic Biology

Available Since

July 2, 2020

Availability

Academic Institutions and Nonprofits only

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PBS Actb-2A-TIR1-GFP

Plasmid

#113832

Purpose

targeting osTIR1-GFP into mouse Actb locus for auxin inducible degradation

Depositor

Janet Rossant

Article

Gu et al Nat Biotechnol. 2018 Jun 11. pii: nbt.41

Insert

osTIR1-GFP (NEWENTRY Synthetic)

Use

Synthetic Biology

Available Since

Aug. 30, 2018

Availability

Academic Institutions and Nonprofits only

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IVT-MCP-MMLV-RT

Plasmid

#207457

Purpose

in vitro transcription of mRNA

Depositor

Erik Sontheimer

Article

Liu et al Nat Biotechnol. 2023 Sep 14. doi: 10.103

Insert

MCP-RT

Expression

Bacterial

Available Since

Nov. 28, 2023

Availability

Academic Institutions and Nonprofits only

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Tet-O-FUW-Zfp536

Plasmid

#45845

Depositor

Marius Wernig

Article

Yang et al Nat Biotechnol. 2013 May;31(5):434-9. do

Insert

Zfp536 (Zfp536 Mouse)

Use

Lentiviral

Expression

Mammalian

Promoter

Tet-ON

Available Since

July 8, 2013

Availability

Academic Institutions and Nonprofits only

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pJ211_WT_low

Plasmid

#128848

Purpose

Low copy number plasmid for expression of E. coli flagellin from its natural promoter

Depositor

Mark Pallen

Article

Thomson et al Curr Res Biotechnol. 2020 Nov;2:45-52. d

Insert

Flagellin

Expression

Bacterial

Available Since

Feb. 12, 2020

Availability

Academic Institutions and Nonprofits only

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pFW11-ffluc

Plasmid

#128487

Purpose

Allelic exchange plasmid to to integrate firefly luciferase gene into S. pyogenes for biophotonic imaging

Depositor

Thomas Proft

Article

Loh et al Appl Microbiol Biotechnol. 2013 Nov;97(2

Insert

firefly luciferase gene

Use

Allelic replacement vector

Tags

spy0535 downstream flanking region and spy0535 up…

Promoter

synthetic

Available Since

Feb. 18, 2020

Availability

Academic Institutions and Nonprofits only

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pTKhc-yibDp-GFP-cp6

Plasmid

#134938

Purpose

Low phosphate inducible circular permutation of GFP

Depositor

Michael Lynch

Article

Menacho-Melgar et al Biotechnol Bioeng. 2020 May 22. doi: 10.

Insert

GFPcp6

Tags

His tag

Expression

Bacterial

Available Since

May 5, 2020

Availability

Academic Institutions and Nonprofits only

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pYL322d1/eGFP-C

Plasmid

#183152

Purpose

Plant transient expression vector containing eGFP tag. Unique nucleotide sequences Uc and Ud for cloning at C terminus of eGFP.

Depositor

Yao-Guang Liu, Qinlong Zhu

Article

Han et al Plant Biotechnol J. 2022 Feb 18. doi: 10

Type

Empty backbone

Expression

Plant

Available Since

April 21, 2022

Availability

Academic Institutions and Nonprofits only

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pCfB4787

Plasmid

#106147

Purpose

EasyCloneYALI system-based yeast integrative vector carrying loxP-flanked nourseothricin-resistance marker, integration into Yarrowia lipolytica chromosomal location IntE_4, USER site for cloning, amp resistance

Depositor

Irina Borodina

Article

Holkenbrink et al Biotechnol J. 2018 Jan 29. doi: 10.1002/

Type

Empty backbone

Expression

Yeast

Available Since

April 6, 2018

Availability

Academic Institutions and Nonprofits only

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Rc/CMV cyclin B2

Plasmid

#10911

Depositor

Bob Weinberg

Article

Hinds et al Cell. 1992 Sep 18. 70(6):993-1006.

Insert

cyclin B2 (CCNB2 Human)

Expression

Mammalian

Available Since

Jan. 5, 2006

Availability

Academic Institutions and Nonprofits only
LV anti-FITC-LaG16-Y186F-T2A-BFP

Plasmid

#194931

Purpose

LV construct encoding Y186F mutant sNRR-based SynNotch receptor

Depositor

John Ngo

Article

Sloas et al Nat Biotechnol. 2023 Jan 16. doi: 10.103

Insert

antiFITC-LaG16-sNRR(Y186F)-SynNotch-Gal4-VP64-T2A-BFP

Use

Lentiviral and Synthetic Biology

Available Since

March 3, 2023

Availability

Academic Institutions and Nonprofits only

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pET21a.Tat(48-57)-I-SceI

Plasmid

#165041

Purpose

Expression of Tat(48-57) conjugated I-SceI for protein purification (6XHis tag)

Depositor

Dokyun Na

Article

Lee et al Commun Biol. 2021 Feb 15;4(1):205. doi:

Insert

I-SceI

Tags

6XHis and Tat(48-57)

Expression

Bacterial

Available Since

Feb. 19, 2021

Availability

Academic Institutions and Nonprofits only

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pMK408 (mAID-Nluc Hygro)

Plasmid

#121198

Purpose

C-terminal tagging

Depositor

Masato Kanemaki

Article

Yesbolatova et al Methods. 2019 Apr 23. pii: S1046-2023(18

Insert

mAID-Nluc Hygro

Use

CRISPR

Available Since

March 11, 2019

Availability

Academic Institutions and Nonprofits only

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pCfB6574

Plasmid

#106132

Purpose

EasyCloneYALI system-based yeast integrative vector carrying loxP-flanked hygromycin-resistance marker, integration into Yarrowia lipolytica chromosomal location IntE_1, USER site for cloning, amp resistance

Depositor

Irina Borodina

Article

Holkenbrink et al Biotechnol J. 2018 Jan 29. doi: 10.1002/

Type

Empty backbone

Expression

Yeast

Available Since

April 6, 2018

Availability

Academic Institutions and Nonprofits only

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pENTR-L1-PGK-TKNeo-L2

Plasmid

#141008

Purpose

Mutisite Gateway mediated vector construction

Depositor

Hideyuki Okano

Article

Yoshimatsu et al PLoS One. 2019 Aug 27;14(8):e0221164. do

Insert

TKNeo

Expression

Mammalian

Available Since

July 8, 2020

Availability

Academic Institutions and Nonprofits only

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pMJA180

Plasmid

#128538

Purpose

Expresses E coli Bir A protein

Depositor

Marcos Alcocer

Article

de la Cruz et al J Biotechnol. 2016 Jun 10;227:112-9. doi

Insert

E coli BirA

Expression

Yeast

Promoter

AOX1

Available Since

Aug. 9, 2019

Availability

Academic Institutions and Nonprofits only

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Rc/CMV cyclin D1

Plasmid

#8962

Depositor

Bob Weinberg

Article

Hinds et al Cell. 1992 Sep 18. 70(6):993-1006.

Insert

cyclin D1 (CCND1 Human)

Expression

Mammalian

Available Since

Nov. 14, 2005

Availability

Academic Institutions and Nonprofits only
pCellFree_G01

Plasmid

#67135

Purpose

Gateway destination vector for cell-free expression with N-term 8xHis tag

Depositor

Kirill Alexandrov

Article

Gagoski et al J Biotechnol. 2015 Feb 10;195:1-7. doi:

Type

Empty backbone

Use

Cell-free expression

Tags

His

Available Since

Aug. 14, 2015

Availability

Academic Institutions and Nonprofits only

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pFREE2-SpCas9-sgRNA

Plasmid

#179582

Purpose

Expresses spCas9 and gRNA that targets ColE1 plasmids for curing

Depositor

Kevin Solomon

Article

Lee et al ACS Synth Biol. 2022 Jan 21;11(1):53-60.

Insert

Cas9

Expression

Bacterial

Available Since

March 15, 2022

Availability

Academic Institutions and Nonprofits only

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p3A9cbetaTCR

Plasmid

#69600

Purpose

TCR beta chain transgene expression plasmid which has a unique ClaI site for the insertion of a TCR beta chain VDJ exon

Depositor

Francis R. Carbone

Article

Barnden et al Immunol Cell Biol. 1998 Feb;76(1):34-40.

Type

Empty backbone

Expression

Mammalian

Available Since

Feb. 23, 2016

Availability

Academic Institutions and Nonprofits only
pEC2735 (pSpy0C2)

Plasmid

#220279

Purpose

integrative plasmid for heterologous gene expression in S. pyogenes SF370, integrates into the amyA locus via homologous recombination

Depositor

Emmanuelle Charpentier

Article

Lautenschlager et al Front Bioeng Biotechnol. 2024 Jun 7;12:1

Type

Empty backbone

Expression

Bacterial

Available Since

Sept. 3, 2024

Availability

Academic Institutions and Nonprofits only

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pIM1708-surT-PsurP-agaN-pNW33N

Plasmid

#44009

Depositor

Ichiro Matsumura

Article

Blanchard et al Appl Microbiol Biotechnol. 2014 May 2.

Insert

surT-PsurP-agaN

Expression

Bacterial

Mutation

Deleted EcoRI site (synonymous change, C to T at …

Available Since

May 15, 2014

Availability

Academic Institutions and Nonprofits only

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PET28a-cpLumiLuc-Rapamycin sensor

Plasmid

#186292

Purpose

Expression Rapamycin sensor in e coli

Depositor

Kirill Alexandrov

Article

Guo et al Nat Commun. 2022 Feb 10;13(1):789. doi:

Insert

cpLumiLuc-Rapamycin sensor

Tags

HisTag

Expression

Bacterial

Available Since

Aug. 26, 2022

Availability

Academic Institutions and Nonprofits only

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pTK297

Plasmid

#59566

Purpose

SIN SP6 P1234 (nsP2 P726S) SGP(14) Kozak mKate-G8-L7Ae 4xFF4

Depositor

Ron Weiss

Article

Wroblewska et al Nat Biotechnol. 2015 Aug 3. doi: 10.1038

Insert

SGP(14) Kozak mKate-G8-L7Ae 4xFF4

Available Since

Aug. 3, 2015

Availability

Academic Institutions and Nonprofits only

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