We narrowed to 24,887 results for: Spr

1. G1397 DddAtox-N–dSpCas9

   Plasmid

   #157835

   Purpose

   expresses split DddAtox-Cas9 construct in mammalian cells

   Depositor

   David Liu

   Article

   Mok et al Nature. 2020 Jul 8. pii: 10.1038/s41586-

   Insert

   bpNLS–G1397 DddAtox-N–dSpCas9–UGI–UGI–bpNLS
   

   Use

   CRISPR

   Available Since

   Aug. 24, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAcrVIB1

   Plasmid

   #184566

   Purpose

   Inducible expression of anti-CRISPR protein AcrVIB1 in bacteria.

   Depositor

   Chase Beisel

   Article

   Wandera et al Mol Cell. 2022 May 24. pii: S1097-2765(2

   Insert

   anti-CRISPR protein inhibiting Cas13b nucleases
   

   Expression

   Bacterial

   Promoter

   T7 promoter

   Available Since

   Oct. 6, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pX330-UGCG-KO

   Plasmid

   #80010

   Purpose

   gRNA to knock out expression of UGCG gene. The product of this gene catalyzes the first step in synthesis of glycosphingolipids.

   Depositor

   Sriram Neelamegham

   Article

   Stolfa et al Sci Rep. 2016 Jul 26;6:30392. doi: 10.10

   Insert

   UGCG (UGCG Human)
   

   Use

   CRISPR

   Available Since

   July 25, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. BPK2301

   Plasmid

   #65778

   Purpose

   Human expression plasmid for S. thermophilus 1 Cas9 sgRNA (need to clone in spacer into BsmBI sites): U6-BsmBIcassette-St1-sgRNA

   Depositor

   Keith Joung

   Article

   Kleinstiver et al Nature. 2015 Jun 22. doi: 10.1038/nature

   Insert

   St1Cas9 gRNA backbone, without spacer sequence
   

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   June 22, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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5. A3Bctd-max

   Plasmid

   #198888

   Purpose

   Express A3Bctd-max BE4max-based base editor

   Depositor

   Reuben Harris

   Article

   Rieffer et al CRISPR J. 2023 Oct;6(5):430-446. doi: 10

   Insert

   Apolipoprotein B mRNA Editing Enzyme, Catalytic Polypeptide-like 3B C-terminal domain (APOBEC3B Human)
   

   Tags

   6x His and NLS

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Nov. 6, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. NAX_CAGGS_CAS9_m4_KRAB-MeCP2_EYFP

   Plasmid

   #167898

   Purpose

   PiggyBac compatible plasmid expressing dCas9-KRAB-MeCP2 expression plasmid

   Depositor

   Alejandro Chavez, Nan Cher Yeo

   Article

   Nghi Dang et al Synthetic Biology, Volume 6, Issue 1, 20

   Insert

   dCas9-KRAB-MeCP2
   

   Use

   CRISPR

   Mutation

   D10A, D839A, H840A, N863A

   Available Since

   Sept. 14, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. sg14x(MS2) MUC4.1

   Plasmid

   #101153

   Purpose

   gRNA with 14 MCP binding site

   Depositor

   Mazhar Adli

   Article

   Qin et al Nat Commun. 2017 Mar 14;8:14725. doi: 10

   Insert

   MUC4 (MUC4 Human)
   

   Use

   CRISPR and Lentiviral

   Available Since

   Nov. 9, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCAG-Cas13d-T2A-GFP(1-10)

   Plasmid

   #224566

   Purpose

   Ubiquitous expression plasmid, contains CAG promoter (CMV immediate early enhancer and chicken beta actin promoter), Cas13d, and split GFP(1-10) reporter.

   Depositor

   Erica Hutchins

   Article

   Kim et al Dev Biol. 2024 Nov 30:S0012-1606(24)0026

   Insert

   Cas13d-T2A-GFP(1-10)
   

   Use

   CRISPR

   Tags

   T2A-split GFP(1-10)

   Available Since

   Dec. 9, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pY014 (pcDNA3.1-hMbCpf1)

   Plasmid

   #69986

   Purpose

   Expresses humanized MbCpf1

   Depositor

   Feng Zhang

   Article

   Zetsche et al Cell. 2015 Sep 23. pii: S0092-8674(15)01

   Insert

   hMbCpf1
   

   Use

   CRISPR

   Tags

   NLS-3xHA

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Oct. 8, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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10. pEJS468-pLK.O1-NmeSgRNA/DTS13-Telomere

    Plasmid

    #85714

    Purpose

    Telomere-targeting Nme sgRNA under U6 promoter

    Depositor

    Erik Sontheimer

    Article

    Pawluk et al Cell. 2016 Dec 15;167(7):1829-1838.e9. d

    Insert

    telomere sgRNA
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Jan. 25, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. pU6-(BbsI)sgRNA_CAG-Cas9-bpA_EF1-TagRFP

    Plasmid

    #86987

    Purpose

    For cloning and expression of sgRNA together with expression of Cas9 and TagRFP

    Depositor

    Ralf Kuehn

    Article

    Yumlu et al Methods Mol Biol. 2019;1961:137-151. doi

    Insert

    Cas9
    

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    April 3, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. MiniCoopR U6:gRNA cdkn2a, mitfa:Cas9

    Plasmid

    #118842

    Purpose

    Targets cdkn2a and expresses zebrafish mitfa specifically in zebrafish melanocytes

    Depositor

    Leonard Zon

    Article

    Ablain et al Science. 2018 Nov 1. pii: science.aau650

    Insert

    cdkn2a gRNA (LOC100329528 Zebrafish)
    

    Use

    CRISPR; Tol2

    Available Since

    Nov. 28, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. MSP977

    Plasmid

    #65774

    Purpose

    Human expression vector for SpCas9 D1135E variant: CMV-T7-humanSpCas9(D1135E)-NLS-3xFLAG

    Depositor

    Keith Joung

    Article

    Kleinstiver et al Nature. 2015 Jun 22. doi: 10.1038/nature

    Insert

    mammalian codon-optimized Streptococcus pyogenes Cas9 (D1135E)-NLS-3XFlag
    

    Use

    CRISPR

    Tags

    3x FLAG and NLS

    Expression

    Mammalian

    Mutation

    D1135E mutation in Cas9

    Promoter

    CMV & T7

    Available Since

    June 22, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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14. pAAV-SMVP-Cas9N-U6-gRNA TdR

    Plasmid

    #80940

    Purpose

    Expresses Cas9N in mammalian cells; expresses gRNA TdR for Ai9 cleavage.

    Depositor

    George Church

    Article

    Chew et al Nat Methods. 2016 Sep 5. doi: 10.1038/nm

    Insert

    Cas9N
    

    Use

    AAV, CRISPR, and Synthetic Biology

    Expression

    Mammalian

    Available Since

    Oct. 3, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCAG-Cas13d-T2A-Citrine

    Plasmid

    #224565

    Purpose

    Ubiquitous expression plasmid, contains CAG promoter (CMV immediate early enhancer and chicken beta actin promoter), Cas13d, and Citrine reporter.

    Depositor

    Erica Hutchins

    Article

    Kim et al Dev Biol. 2024 Nov 30:S0012-1606(24)0026

    Insert

    Cas13d-T2A-Citrine
    

    Use

    CRISPR

    Tags

    T2A-Citrine

    Available Since

    Dec. 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pMK-Cas9-gate

    Plasmid

    #113743

    Purpose

    Gateway destination vector encoding Maize ubiquitin promoter driving Cas9 and 35S promoter driving aminoglycoside phosphotransferase for G418 selection in plants.

    Depositor

    Magdalena Bezanilla

    Article

    Mallett et al Plant Direct. 2019 Sep 12;3(9):e00168. d

    Insert

    Cas9
    

    Use

    CRISPR; Gateway destination vector

    Promoter

    Z. Mays ubiquitin promoter, 35S promoter

    Available Since

    Sept. 24, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. pU6T7G

    Plasmid

    #71463

    Purpose

    Expresses gRNA from hybrid human U6/T7 promoter for both cellular expression and in vitro transcription from same promoter (1 G at initiation)

    Depositor

    Willie Mark

    Article

    Romanienko et al PLoS One. 2016 Feb 5;11(2):e0148362. doi

    Type

    Empty backbone

    Use

    CRISPR

    Expression

    Bacterial and Mammalian

    Promoter

    human U6/phage T7

    Available Since

    Feb. 9, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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18. pLenti-U6sgbbBsmbI-puro-2A-Fluc

    Plasmid

    #100277

    Purpose

    Lentiviral vector for CRISPR mediated gene editing in cells while expressing Firefly Luciferase for in vivo imaging

    Depositor

    Sidi Chen

    Article

    Chow et al Nat Neurosci. 2017 Aug 14. doi: 10.1038/

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Available Since

    Oct. 26, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. pRB17

    Plasmid

    #52527

    Purpose

    serves as template for PCR-mediated fusion of U6-promoter with sgRNA

    Depositor

    Klaus Foerstemann

    Article

    Bottcher et al Nucleic Acids Res. 2014 Apr 19.

    Insert

    Drosophila U6C promoter
    

    Use

    CRISPR; Blunt cloning kit

    Tags

    T7-promoter

    Expression

    Insect

    Available Since

    April 14, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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20. pMpGE006

    Plasmid

    #108722

    Purpose

    Cas9 expression in M.polymorpha

    Depositor

    Takayuki Kohchi, Shigeo Sugano

    Article

    Sugano et al PLoS One. 2018 Oct 31;13(10):e0205117. d

    Insert

    Atco-Cas9-Pea3ter
    

    Use

    CRISPR

    Expression

    Plant

    Promoter

    MpEFpro

    Available Since

    June 1, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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