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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 14,273 results for: cas9

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Showing: 3301 - 3320 of 14273 results

  1. pUC19-A7HP89

    Plasmid
    #103082
    Purpose
    Cas9 coding gene template with optimized sequence for human codon usage
    Depositor
    Duhee Bang
    Article
    Cho et al Nucleic Acids Res. 2018 May 18;46(9):e55
    Insert
    A7HP89(Cas9 coding gene from Parvibaculum lavamentivorans (strain DS-1 / DSM 13023 / NCIMB 13966))
    Use
    CRISPR; Cloning vector
    Mutation
    human codon-optimized
    Available Since
    April 20, 2018
    Availability
    Academic Institutions and Nonprofits only
    103082-plasmid-map-sequence-id-201722

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  6. AAV:ITR-U6-sgRNA(NeuN)-hSyn-Cre-2A-EGFP-KASH-WPRE-shortPA-ITR

    Plasmid
    #60230
    Purpose
    Expresses Cre recombinase and KASH-tagged EGFP from the hSyn promoter and one U6-driven sgRNA. AAV backbone with SapI spacer for sgRNA cloning.
    Depositor
    Feng Zhang
    Article
    Platt et al Cell. 2014 Sep 24. pii: S0092-8674(14)01
    Inserts
    sgRNA
    Cre recombinase
    EGFP
    Use
    AAV, CRISPR, Cre/Lox, and Mouse Targeting
    Tags
    Cre-HA-P2A and EGFP-KASH
    Expression
    Mammalian
    Promoter
    U6 and hSyn
    Available Since
    Nov. 3, 2014
    Availability
    Academic Institutions and Nonprofits only
    60230-plasmid-map-sequence-id-490567

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  7. pdCas9-DNMT3A-PuroR_BACH2-sgRNA8 (ANV)

    Plasmid
    #71829
    Purpose
    Expression of mutant dCas9-DNMT3A fusion (inactive catalytic domain of DNMT3A) with T2A-PuroR and specific sgRNA for the human BACH2 promoter; for use as a control
    Depositor
    Vlatka Zoldos
    Article
    Vojta et al Nucleic Acids Res. 2016 Mar 11. pii: gkw
    Insert
    BACH2-sgRNA8 (BACH2 Synthetic, S. pyogenes, Human)
    Use
    CRISPR
    Tags
    3xFLAG, SV40 NLS, and T2A-PuroR
    Expression
    Mammalian
    Mutation
    D10A and H840A in S.pyogenes Cas9; E756A inactiva…
    Promoter
    U6
    Available Since
    March 7, 2016
    Availability
    Academic Institutions and Nonprofits only
    71829-plasmid-map-sequence-id-131024

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  9. pML104-NatMx3

    Plasmid
    #83477
    Purpose
    Expresses Cas9 and contains guide RNA expression cassette with BclI-SwaI cloning sites for guide sequence cloning; Contains NatMX3 marker for yeast transformation.
    Depositor
    Patricia Wittkopp
    Insert
    NatMx3
    Expression
    Yeast
    Available Since
    Oct. 5, 2016
    Availability
    Academic Institutions and Nonprofits only

  10. pEGB2Ω2_SF-35s:hCas9:tNos (GB1103)

    Plasmid
    #75400
    Purpose
    Transcriptional unit for (human codon optimized) Cas9 plant expression driven by the 35S promoter. Specially conceived to be linked with the TU of the kanamycin resistance gene GB1181.
    Depositor
    Diego Orzaez
    Article
    Vazquez-Vilar et al Plant Methods. 2016 Feb 1;12:10. doi: 10
    Insert
    hCas9
    Use
    CRISPR and Synthetic Biology
    Expression
    Plant
    Promoter
    35S
    Available Since
    Aug. 5, 2016
    Availability
    Academic Institutions and Nonprofits only
    75400-plasmid-map-sequence-id-146044

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  17. p-dCas9-JMJD2A-S288G-T289G-Hygro

    Plasmid
    #104418
    Purpose
    transient expression of dCas9-dJMJD2A fusion protein
    Depositor
    Stephan Beck
    Insert
    dJMJD2A
    Use
    CRISPR
    Expression
    Mammalian
    Mutation
    S288G-T289G
    Promoter
    CMV promoter
    Available Since
    April 2, 2020
    Availability
    Academic Institutions and Nonprofits only
    104418-plasmid-map-sequence-id-204359

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  18. p-dCas9-dTET1-H1672Y-D1674A-Hygro

    Plasmid
    #104413
    Purpose
    transient expression of dCas9-dTET1 fusion protein
    Depositor
    Stephan Beck
    Insert
    dTET1
    Use
    CRISPR
    Expression
    Mammalian
    Mutation
    H1672Y-D1674A
    Promoter
    CMV promoter
    Available Since
    April 2, 2020
    Availability
    Academic Institutions and Nonprofits only
    104413-plasmid-map-sequence-id-204141

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Showing: 3301 - 3320 of 14273 results