163,347 results

1. BII-Sh-FUCCI-Luc

   Plasmid

   #133364

   Purpose

   Piggybac vector for shRNA-mediated knockdown, co-expressed with FUCCI cell cycle reporter

   Depositor

   Blair Madison

   Article

   Fischer et al Am J Physiol Gastrointest Liver Physiol.

   Insert

   Clover-Geminin-IRES-mKO2-Cdt1
   

   Use

   Tags

   HA-tagged Clover-Geminin

   Expression

   Mammalian

   Mutation

   N/A

   Promoter

   CMV enhancer and hEf1a (CpG free)

   Available since

   Jan. 3, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pTYF-super-mfPENK-0.9k-Venus-WPRE

   Plasmid

   #156401

   Purpose

   Expresses Venus under PENK gene promoter

   Depositor

   Shuji Kaneko

   Article

   Nagai et al Biochem Biophys Res Commun. 2019 Oct 22;

   Inserts

   Venus
   Gal4p65
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Sept. 17, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pGP-CMV-NEW-fRGECO2

   Plasmid

   #125248

   Purpose

   Expresses red fluorescent calcium sensor in mammalian cells

   Depositor

   Katalin Torok

   Article

   Kerruth et al J Biol Chem. 2019 Mar 15;294(11):3934-39

   Insert

   fRGECO2
   

   Use

   Tags

   NES signal

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Nov. 25, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pET-45b(+)-Tn5

   Plasmid

   #112112

   Purpose

   Expresses hyperactive Tn5 transposase for next generation sequencing library preparation

   Depositor

   Frank Pugh

   Article

   Rossi et al Nat Commun. 2018 Jul 20;9(1):2842. doi:

   Insert

   Tn5
   

   Use

   Tags

   His6

   Expression

   Bacterial

   Mutation

   E54K E110K P242A L372P

   Promoter

   Available since

   July 10, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pENN.AAV.hSyn.Cre.hGH (AAV9)

   Viral Prep

   #105555-AAV9

   Purpose

   Ready-to-use AAV9 particles produced from pENN.AAV.hSyn.Cre.hGH (#105555). In addition to the viral particles, you will also receive purified pENN.AAV.hSyn.Cre.hGH plasmid DNA. hSyn driven Cre expression. These AAV preparations are suitable purity for injection into animals.

   Depositor

   James M. Wilson

   Promoter

   Syn

   Tags

   None

   Available since

   July 26, 2018

   Availability

   Academic Institutions and Nonprofits only

6. TFORF0098

   Plasmid

   #142460

   Purpose

   Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

   Depositor

   Feng Zhang

   Article

   Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

   Insert

   MSGN1 (MSGN1 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   April 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. siRNA-resistant ABHD16A-Halo

   Plasmid

   #195163

   Purpose

   mammalian expression of siRNA-resistant ABHD16A tagged with Halo tag

   Depositor

   Gia Voeltz

   Article

   Nguyen et al Elife. 2022 Nov 30;11:e84279. doi: 10.75

   Insert

   ABHD16A (ABHD16A Human)
   

   Use

   Tags

   Halo tag

   Expression

   Mammalian

   Mutation

   Wobble mutations for siRNA resistance from L90 to…

   Promoter

   CMV

   Available since

   Feb. 7, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pCfB4586

   Plasmid

   #106124

   Purpose

   EasyCloneYALI system-based yeast integrative vector carrying loxP-flanked Ura3 marker, integration into Yarrowia lipolytica chromosomal location IntB, USER site for cloning, amp resistance

   Depositor

   Irina Borodina

   Article

   Holkenbrink et al Biotechnol J. 2018 Jan 29. doi: 10.1002/

   Type

   Empty backbone

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   May 11, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pDEST-eGFP-C1-VPS35

   Plasmid

   #163622

   Purpose

   human VPS35 cDNA C-terminally tagged with eGFP

   Depositor

   Josef Kittler

   Article

   Halff et al Cell Rep. 2019 Nov 26;29(9):2599-2607.e6

   Insert

   human VPS35 (VPS35 Human)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 15, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pDONR221-SLC39A7_STOP

    Plasmid

    #161331

    Purpose

    Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors. Contains a STOP codon at the end of the codon-optimized ORF sequence.

    Depositor

    Giulio Superti-Furga, RESOLUTE Consortium

    Article

    Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

    Insert

    SLC39A7 (SLC39A7 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 15, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

11. pDONR221-SLC39A11_STOP

    Plasmid

    #161307

    Purpose

    Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors. Contains a STOP codon at the end of the codon-optimized ORF sequence.

    Depositor

    Giulio Superti-Furga, RESOLUTE Consortium

    Article

    Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

    Insert

    SLC39A11 (SLC39A11 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 15, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

12. pDONR221-SLC39A13_STOP

    Plasmid

    #161283

    Purpose

    Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors. Contains a STOP codon at the end of the codon-optimized ORF sequence.

    Depositor

    Giulio Superti-Furga, RESOLUTE Consortium

    Article

    Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

    Insert

    SLC39A13 (SLC39A13 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 15, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

13. pECO300

    Plasmid

    #169766

    Purpose

    Express SpCas9 and Kanamycin resistance gene

    Depositor

    Sang-Gyu Kim

    Article

    Oh et al Plant Methods. 2020 Mar 12;16:37. doi: 1

    Type

    Empty backbone

    Use

    Plant binary vector

    Tags

    Expression

    Plant

    Mutation

    Promoter

    35S, AtU6

    Available since

    March 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pGRNA3e

    Plasmid

    #169783

    Purpose

    Insert one gRNA

    Depositor

    Sang-Gyu Kim

    Article

    Oh et al Plant Methods. 2020 Mar 12;16:37. doi: 1

    Type

    Empty backbone

    Use

    Unspecified

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pGRNA2

    Plasmid

    #169779

    Purpose

    Insert one gRNA

    Depositor

    Sang-Gyu Kim

    Article

    Oh et al Plant Methods. 2020 Mar 12;16:37. doi: 1

    Type

    Empty backbone

    Use

    Unspecified

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. 240 pTol2005b

    Plasmid

    #73483

    Purpose

    Tol2 vector with multi cloning site

    Depositor

    Nathan Lawson

    Article

    Villefranc et al Dev Dyn. 2007 Nov . 236(11):3077-87.

    Type

    Empty backbone

    Use

    Tol2

    Tags

    Expression

    Mutation

    Promoter

    none

    Available since

    Feb. 25, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. Stat1 alpha Flag pRc/CMV

    Plasmid

    #8691

    Depositor

    Jim Darnell

    Article

    Horvath et al Genes Dev 1995 Apr 15;9(8):984-94.

    Insert

    STAT1 alpha (STAT1 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 13, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pTF263 (pFA6a-TEV-6xGly-12xHis-His3MX)

    Plasmid

    #44069

    Depositor

    Tim Formosa

    Type

    Empty backbone

    Use

    Pcr-based yeast c-terminal tagging

    Tags

    12xHis tag, 6xGly linker, ADH1 transcription term…

    Expression

    Mutation

    Promoter

    Available since

    April 2, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pCDH-Flag-c-Myc

    Plasmid

    #102626

    Purpose

    Expresses c-Myc in mammalian cells

    Depositor

    Hening Lin

    Article

    Jing et al Cancer Cell. 2016 Mar 14;29(3):297-310.

    Insert

    MYC proto-oncogene, bHLH transcription factor (MYC Human)
    

    Use

    Lentiviral

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 30, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. PIK3C2GA

    Plasmid

    #42451

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Nicola Burgess-Brown

    Insert

    PIK3C2GA (PIK3C2G Human)
    

    Use

    Tags

    His6-TEV

    Expression

    Bacterial

    Mutation

    See Comments

    Promoter

    Available since

    March 18, 2013

    Availability

    Academic Institutions and Nonprofits only

21. pBFC0984

    Plasmid

    #231992

    Purpose

    CRISPRi-ART plasmid encoding aTc-inducible dRfxCas13d and constitutive crRNA with 2xBsaI spacer cloning site

    Depositor

    Brady Cress

    Article

    Adler et al Nat Microbiol. 2025 Mar;10(3):694-709. d

    Inserts

    dRfxCas13d
    crRNA with 2xBsaI spacer
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    J23119 and pTet

    Available since

    April 7, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pNGFP-BC

    Plasmid

    #161956

    Purpose

    Used for rapid and efficient membrane protein precrystallization screening strategy utilizing green fluorescent protein and fluorescence-detection size-exclusion chromatography (FSEC).

    Depositor

    Eric Gouaux

    Article

    Kawate et al Structure. 2006 Apr;14(4):673-81. doi: 1

    Type

    Empty backbone

    Use

    Tags

    GFPuv His8

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    July 15, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pCGFP-BC

    Plasmid

    #161959

    Purpose

    Used for rapid and efficient membrane protein precrystallization screening strategy utilizing green fluorescent protein and fluorescence-detection size-exclusion chromatography (FSEC).

    Depositor

    Eric Gouaux

    Article

    Kawate et al Structure. 2006 Apr;14(4):673-81. doi: 1

    Type

    Empty backbone

    Use

    Tags

    GFPuv His8

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    July 15, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pMC-Dest-BsaI

    Plasmid

    #200550

    Purpose

    Mini circle destination vector with BsaI sites for Golden Gate Assembly

    Depositor

    Junsu Kang

    Type

    Empty backbone

    Use

    Minicircle cloning vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 3, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. 6xHsa.NFKB:d2mRFP1

    Plasmid

    #239991

    Purpose

    Destabilized red fluorescent protein (d2mRFP1) under transcriptional control of NF-kB activation

    Depositor

    Raquel Espin-Palazon

    Inserts

    mRFP1
    PEST
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    cfos minimal promoter

    Available since

    June 27, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pcDNA-mito-GreenPy1Highest

    Plasmid

    #239069

    Purpose

    Mammalian expression of mitochondria-targeted highest affinity green fluorescent pyruvate biosensor GreenPy1Highest

    Depositor

    Robert Campbell

    Article

    Imai et al bioRxiv 2025.04.17.649293

    Insert

    GreenPy1Highest
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 24, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. GFP-LRRK2

    Plasmid

    #232872

    Purpose

    Expresses LRRK2 in mammalian cells

    Depositor

    Pietro De Camilli

    Article

    Wang et al Nat Cell Biol. 2025 May;27(5):776-789. d

    Insert

    GFP-LRRK2 (LRRK2 Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 24, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pcDNA-GreenPy1Highest

    Plasmid

    #239059

    Purpose

    Mammalian expression of highest affinity green fluorescent pyruvate biosensor GreenPy1Highest

    Depositor

    Robert Campbell

    Article

    Imai et al bioRxiv 2025.04.17.649293

    Insert

    GreenPy1Highest
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 18, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pcDNA-mito-ApplePy1Highest

    Plasmid

    #239074

    Purpose

    Mammalian expression of mitochondria-targeted highest affinity red fluorescent pyruvate biosensor ApplePy1Highest

    Depositor

    Robert Campbell

    Article

    Imai et al bioRxiv 2025.04.17.649293

    Insert

    ApplePy1Highest
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 18, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pMB-Tl-ADPHK

    Plasmid

    #237469

    Purpose

    Expression of His-tagged Thermococcus litoralis ADP hexokinase

    Depositor

    Michael Baker

    Insert

    ADP hexokinase
    

    Use

    Tags

    C-terminal 10 His-tag and N-terminal strep tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    June 10, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pCDH-3_Flag-YTHDF2-m6A-MUT

    Plasmid

    #238976

    Purpose

    Expresses the m6A-binding-deficient mutant of YTHDF2

    Depositor

    Jianjun Chen

    Article

    Chen et al Cell. 2024 Dec 12:S0092-8674(24)01324-2.

    Insert

    YTHDF2-m6A-MUT (YTHDF2 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    2 site mutation (W432A+W486A)

    Promoter

    Available since

    June 5, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pETCK3 TGP-E

    Plasmid

    #236255

    Purpose

    Expresses TGP-E, a thermostable fluorescent protein

    Depositor

    Natasha DeVore

    Article

    Anderson et al Biochem Mol Biol Educ. 2024 Sep-Oct;52(5

    Insert

    Thermal Green Protein Q66E (TGP-E)
    

    Use

    Tags

    His tag

    Expression

    Bacterial

    Mutation

    Promoter

    lac operon

    Available since

    May 23, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. FLAG-FBXL17

    Plasmid

    #236188

    Purpose

    Expression of N-terminal tagged FLAG-FBXL17 (H. sapiens, codon-optimized) in human cell lines.

    Depositor

    Michele Pagano

    Article

    Cao et al Cell. 2024 Oct 30:S0092-8674(24)01188-7.

    Insert

    FLAG-FBXL17 (FBXL17 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 6, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. FUGW U6 gLacZ dCas9-KRAB-T2a-GFP

    Plasmid

    #234883

    Purpose

    non-targeting CRISPRi control

    Depositor

    Johan Jakobsson

    Article

    Adami et al bioRxiv 2025.01.17.633315

    Insert

    L1HS gRNA
    

    Use

    CRISPR and Lentiviral

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 22, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. GFP-hCGN

    Plasmid

    #236507

    Purpose

    For expression of Cingulin (human) in mammalian cells

    Depositor

    Sandra Citi

    Article

    Vasileva et al J Biol Chem. 2022 Apr;298(4):101797. doi

    Insert

    CGN (CGN Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    WT

    Promoter

    Available since

    April 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. hCGNL1-Myc-HA

    Plasmid

    #236515

    Purpose

    For expression of hParacingulinL1 in mammalian cells

    Depositor

    Sandra Citi

    Article

    Vasileva et al J Biol Chem. 2022 Apr;298(4):101797. doi

    Insert

    CGNL1 (CGNL1 Human)
    

    Use

    Tags

    Myc-HA

    Expression

    Mammalian

    Mutation

    WT

    Promoter

    Available since

    April 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pMXs-mAscl1

    Plasmid

    #233152

    Purpose

    Retroviral expression of Ascl1

    Depositor

    Kate Galloway

    Article

    Wang et al Cell Syst. 2025 Apr 16;16(4):101205. doi

    Insert

    Ascl1 (Ascl1 Mouse)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 13, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. Flag-ZDHHC13

    Plasmid

    #231661

    Purpose

    Expresses human ZDHHC13 protein

    Depositor

    Chunhong Ma

    Article

    Zhang et al Sci Immunol. 2024 Nov 15;9(101):eadp7302

    Insert

    ZDHHC13 (ZDHHC13 Human)
    

    Use

    Tags

    3×Flag Tag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 13, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pAIP4_2WWE

    Plasmid

    #234210

    Purpose

    Heterologous protein expression of PI3K-C2-gamma in Escherichia coli

    Depositor

    Christopher Bahl

    Insert

    2WWE (PIK3C2G Human)
    

    Use

    Tags

    10x HisTag-Smt3

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    March 6, 2025

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

40. pSems StayGold-2xFYVE

    Plasmid

    #222948

    Purpose

    Expression of HRS 2xFYVE tagged with StayGold in mammalian cells

    Depositor

    Jacob Piehler

    Article

    Winkelmann et al Nat Commun. 2024 Jul 10;15(1):5813. doi:

    Insert

    tandem HRS FYVE domain (Hgs Mouse)
    

    Use

    Tags

    StayGold

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 7, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge