We narrowed to 17,478 results for: IGH@
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pASKt15C+SrtA5
Plasmid#64985PurposeExpresses the P94R/D160N/D165A/K190E/K196T mutant of Staphylococcus aureus sortase A in E. coliDepositorInsertsortase A
TagsHis6 tag and S tagExpressionBacterialMutationdeleted amino acids 1-59, P94R, D160N, D165A, K19…Promotertetracycline promoter/operatorAvailable SinceJune 9, 2015AvailabilityAcademic Institutions and Nonprofits only -
NmeCas9_3XNLS_pMCSG7
Plasmid#120078PurposeBacterial expression of wtNmeCas9 with 3X NLSDepositorInsertNmeCas9
ExpressionBacterialAvailable SinceFeb. 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
pWXLd-RIEP-OgNLuc
Plasmid#135936PurposeExpresses the OgNLuc LumiFluor in mammalian cells.DepositorInsertOgNLuc LumiFluor
UseLentiviralExpressionMammalianPromoterEF1aAvailable SinceMarch 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMSCV-Myc-Caspase-4_W267N
Plasmid#214311PurposeExpression of gene in mammalian cells by retroviral transductionDepositorAvailable SinceFeb. 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
pC1.530
Plasmid#205441PurposeLevel 1 Position 1 pUC19 vector with sgRNA and GmR. Used for homologous recombination with Cas12a editing vector pC1.509. sgRNA targets pC1.509 for removal.DepositorInsertRepB gRNA
UseSynthetic BiologyAvailable SinceMay 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
pEF-1α_ZF9LOV[C2]-VP64 (pLZA126)
Plasmid#203911PurposeEncodes engineered opto-ZF9 with AsLOV2(408-543) inserted in C2 position in mammalian cellsDepositorInsertZF9LOV[C2]-VP64
ExpressionMammalianPromoterEF1αAvailable SinceJuly 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
pEF-1α_ZF9LOV[C1]-VP64 (pLZA125)
Plasmid#203910PurposeEncodes engineered opto-ZF9 with AsLOV2(408-543) inserted in C1 position in mammalian cellsDepositorInsertZF9LOV[C1]-VP64
ExpressionMammalianPromoterEF1αAvailable SinceJuly 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
907H1
Plasmid#160234PurposeFlightN-CymR-VP16-3X-SV40-attBDepositorInsertsp-cymene repressor
Flightin promoter
TagsVP16ExpressionInsectAvailable SinceNov. 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
IDP466
Plasmid#87008Purposegenomic Sindbis virus plasmid for expression of SYNseq componentsDepositorInsertsmyc-3xFlag-CLIP-Nrx1B-1xλN(i)
eGFP
UseSynthetic Biology; Sindbis virus genomic plasmidExpressionMammalianAvailable SinceFeb. 23, 2017AvailabilityAcademic Institutions and Nonprofits only -
pET21a - APX
Plasmid#89618PurposeExpresses recombinant 6HIS tagged T. cruzi ascorbate-dependent tryparedoxin peroxidase protein in E. coli upon IPTG inductionDepositorInsertAscorbate dependent Tryparedoxin peroxidase
Tags6HIS and T7 tag (gene 10 leader)ExpressionBacterialAvailable SinceMay 5, 2017AvailabilityAcademic Institutions and Nonprofits only -
Nano-lantern(Ca2+)_600/pcDNA3
Plasmid#51982Purposemammalian expression of a Nano-lantern-based luminescent Ca2+ indicator with Kd=600nMDepositorInsertNano-lantern_CaM_E104Q-2G
ExpressionMammalianMutationS257G in Rluc8Available SinceApril 15, 2014AvailabilityAcademic Institutions and Nonprofits only -
mc2 blast EF1a
Plasmid#206223PurposeFor expressing cloned exons as circular RNA. Alternatively, for expressing cDNA (if the EcoRI-XhoI restriction fragment is also removed).DepositorTypeEmpty backboneUseSynthetic BiologyExpressionMammalianAvailable SinceAug. 31, 2023AvailabilityAcademic Institutions and Nonprofits only -
pORE303N_CEN12RRFuzzy3MYB
Plasmid#194441PurposeCRISPR, Cas9 driven by double 35S, nptII for plant selection, knockout: CEN12, RR, and Fuzzy3MYBDepositorInsertgRNA array targeting CEN1, RR, Fuzzy3MYB
UseCRISPRExpressionPlantPromoterMtU6Available SinceJan. 25, 2023AvailabilityAcademic Institutions and Nonprofits only -
Dendra2-Keratin-17
Plasmid#57726PurposeNote- This plasmid contains Keratin 18. Localization: Cytokeratin, Excitation: 490 / 553, Emission: 507 / 573DepositorAvailable SinceJan. 13, 2015AvailabilityIndustry, Academic Institutions, and Nonprofits -
pCAG-attBr-Cre-rc-attP
Plasmid#51264PurposeComponent of the Bxb1-rcCre binary site-specific recombinase system, reverse complementary Cre CDS flanked by Bxb1 attB/P recognition sites in head-to-head orientationDepositorInsertattBr-Cre-rc-attP
ExpressionMammalianPromoterCAGAvailable SinceMarch 21, 2014AvailabilityAcademic Institutions and Nonprofits only -
pJK03_Rho_basal_DsRed
Plasmid#173490PurposeVector backbone used to clone MPRA librariesDepositorInsertBovine Rhodopsin promoter driving DsRed fluorescent protein
ExpressionMammalianPromoterBovine RhodopsinAvailable SinceMay 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGEX-4T-1-p19-T7-PLK1
Plasmid#46312DepositorInsertsp19
PLK1 DNA-1
linker
PLK1 DNA-2
UsePro-sirnaTags6XHis and GSTExpressionBacterialAvailable SinceJan. 2, 2014AvailabilityAcademic Institutions and Nonprofits only -
miniSOG-Lifeact-7
Plasmid#54823PurposeLocalization: Actin, Excitation: 448 / 473, Emission: 500 / 528DepositorInsertLifeact-miniSOG
TagsLifeactExpressionMammalianAvailable SinceJune 20, 2014AvailabilityIndustry, Academic Institutions, and Nonprofits -
pCMX-PGC1
Plasmid#6DepositorAvailable SinceJune 27, 2005AvailabilityAcademic Institutions and Nonprofits only -
Clover-Lysosomes-20
Plasmid#56528PurposeLocalization: Lysosome Membrane, Excitation: 505, Emission: 515DepositorInsertLAMP1
TagsCloverExpressionMammalianPromoterCMVAvailable SinceDec. 5, 2014AvailabilityIndustry, Academic Institutions, and Nonprofits -
pYFAC-CH1
Plasmid#168977PurposeAMA1 based expression in filamentous fungi. pYFAC-pyrG carrying PalcA ; unique restriction site: PacIDepositorTypeEmpty backboneUseSynthetic Biology; Heterologous expression in fil…Available SinceApril 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
IDP425
Plasmid#87022Purposegenomic Sindbis virus plasmid for use in SYNseq PLA screenDepositorInsertsSNAP-Nlg1AB-1xλN(i)
mCherry
UseSynthetic Biology; Sindbis virus genomic plasmidExpressionMammalianAvailable SinceFeb. 23, 2017AvailabilityAcademic Institutions and Nonprofits only -
AB.pCCL.sin.cPPT.GFP.miR-185-5p.sensor.PGK.dNGFR.WPRE
Plasmid#85930PurposeSensor PlasmidDepositorAvailable SinceJan. 12, 2017AvailabilityAcademic Institutions and Nonprofits only -
pOAD
Plasmid#196825PurposeYeast two-hybrid ; Gal4 activation domainDepositorTypeEmpty backboneTagsGal4 Activation domainExpressionYeastPromoterADHAvailable SinceMay 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
pHW-CIBN-MP-HRW-CRY2-VHH(GFP)
Plasmid#126024Purposeexpress RFP-tagged LARIAT for optogenetic clusteringDepositorInsertCIBN-MP; CRY2 PHR-VHH(GFP)
TagsRFPExpressionInsectMutationWTPromoterHsp70Available SinceSept. 24, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAL189
Plasmid#22278PurposeExpresses Intersectin DH-PH domains fused to mYFP-PIF6, for optogenetic recruitmentDepositorInsertIntersectinDHPH-20aaLinker-mYFP-PIF6APB (PIL2 Mustard Weed)
UseSynthetic BiologyExpressionMammalianPromoterSV40Available SinceOct. 21, 2009AvailabilityAcademic Institutions and Nonprofits only -
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pNS3
Plasmid#131770PurposePADH1_FUSN_Fusion Red_Cry2Olig_TACT1 for Integration into δ-sitesDepositorInsertPADH1_FUSN_Fusion Red_Cry2Olig_TACT1
UseIntegration into δ-sitesExpressionYeastMutationE490G to make Cry2 into Cry2OligPromoterPADH1Available SinceSept. 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
FLAG TBC1D13
Plasmid#87953PurposeExpresses flag tagged mouse TBC1D13DepositorAvailable SinceMay 30, 2017AvailabilityAcademic Institutions and Nonprofits only -
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pOVC1_EcSSPB-nano_X
Plasmid#131212PurposeExpresses EcSSPB-nano (Escherichia coli) followed by restriction sitesDepositorInsertEcSSPB-nano
ExpressionMammalianPromoterCMVAvailable SinceSept. 17, 2019AvailabilityAcademic Institutions and Nonprofits only -
pNS1
Plasmid#131769PurposePADH1_FUSN_Fusion Red_Cry2WT_TACT1 for Integration into δ-sitesDepositorInsertFUSN_Fusion Red_Cry2WT
UseIntegration into δ-sitesExpressionYeastPromoterPADH1Available SinceSept. 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
pGEMHE-mClover3
Plasmid#171497PurposeT7 promotor drives in vitro transcription of mClover3 mRNADepositorInsertmClover3
UsePgehmePromoterT7Available SinceAug. 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
MXS_CMV::NeoR-bGHpA
Plasmid#62437PurposeMXS_chaining vector with CMV::NeoR-bGHpADepositorInsertresistance cassette against Neomycin with CMV Promoter
UseSynthetic BiologyAvailable SinceMay 8, 2015AvailabilityAcademic Institutions and Nonprofits only -
pGEX6p1 AO7RE
Plasmid#137843PurposeGST tagged AO7 encoding aa 126-258 for expression in bacteriaDepositorAvailable SinceMarch 5, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCellFree_G08
Plasmid#67142PurposeGateway destination vector for cell-free expression with C-term mCherry myc-tag tagDepositorTypeEmpty backboneUseCell-free expressionTagsHis-MYC and mCherryAvailable SinceAug. 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
MXS_PGK::PuroR-bGHpA
Plasmid#62440PurposeMXS_chaining vector with PGK::PuroR-bGHpADepositorInsertresistance cassette against Puromycin with PGK Promoter
UseSynthetic BiologyAvailable SinceMay 8, 2015AvailabilityAcademic Institutions and Nonprofits only -
IDP427
Plasmid#87021Purposegenomic Sindbis virus plasmid for use in SYNseq PLA screenDepositorInsertsSNAP-Nlg1AB-tdMS2(i)
mCherry
UseSynthetic Biology; Sindbis virus genomic plasmidExpressionMammalianAvailable SinceFeb. 23, 2017AvailabilityAcademic Institutions and Nonprofits only -
mNG-mTq2
Plasmid#124217PurposeCytoplasmic mNeongreen mTurquoise2 tandem, ~60 % energy transfer. pNM043 - pSAV057-mNG-mTq2DepositorInsertmNG-mTq2
ExpressionBacterialAvailable SinceMay 9, 2019AvailabilityAcademic Institutions and Nonprofits only -
pHH0103_SIAH2_Sina
Plasmid#109813PurposeProtein expression and purification of SIAH2_SinaDepositorInsertSIAH2_Sina (SIAH2 Human)
ExpressionBacterialAvailable SinceOct. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
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jk107
Plasmid#87007Purposeplasmid for cell culture expression of SYNseq componentsDepositorInsertsSNAP-Nlg1AB-1xλN(i)
mCherry
UseSynthetic Biology; Sindbis virus genomic plasmidExpressionMammalianAvailable SinceFeb. 23, 2017AvailabilityAcademic Institutions and Nonprofits only -
mEos2-Calnexin-N-14
Plasmid#57352PurposeLocalization: Endoplasmic Reticulum, Excitation: 505 / 569, Emission: 516 / 581DepositorInsertCalnexin (CANX Human)
TagsmEos2ExpressionMammalianMutationI474N in CalnexinPromoterCMVAvailable SinceFeb. 5, 2015AvailabilityIndustry, Academic Institutions, and Nonprofits -
pAAV-CAG-EGFP barcode-10-SV40 polyA
Plasmid#190874PurposeFor barcoded retrograde labelingDepositorInsertEGFP-barcode10
UseAAVMutationWTAvailable SinceNov. 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAGM7547
Plasmid#169091PurposeMoClo level 1 35S promoter-Db5GT-35S terminator transcription unitDepositorInsert35S promoter-Db5GT-35S terminator
UseMoclo cloning vectorExpressionPlantAvailable SinceJune 22, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAGM7523
Plasmid#169090PurposeMoClo level 1 35S promoter-BvDODA1-Ocs terminator transcription unitDepositorInsert35S promoter-BvDODA1-Ocs terminator
UseMoclo cloning vectorExpressionPlantAvailable SinceJune 22, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAGM7535
Plasmid#169089PurposeMoClo level 1 35S promoter-BvCYP76AD1-Nos terminator transcription unitDepositorInsert35S promoter-BvCYP76AD1-Nos terminator
UseMoclo cloning vectorExpressionPlantAvailable SinceJune 22, 2021AvailabilityAcademic Institutions and Nonprofits only -
pHA-ΔN332-Kin1C
Plasmid#31608DepositorAvailable SinceOct. 4, 2011AvailabilityAcademic Institutions and Nonprofits only -
pFPL-Yh2
Plasmid#61650PurposeGateway vector for generating yellow fluorescent protein fusions in fungiDepositorInsertenhanced yellow fluorescent protein
ExpressionPlantAvailable SinceJune 10, 2015AvailabilityAcademic Institutions and Nonprofits only
