We narrowed to 34,812 results for: Met;

1. M-NMn-VP64

   Plasmid

   #48676

   Purpose

   Mammalian NM-VP64 nuclease-null Cas9 activator expression, human optimized

   Depositor

   George Church

   Article

   Esvelt et al Nat Methods. 2013 Sep 29. doi: 10.1038/n

   Insert

   Cas9-VP64, nuclease-null
   

   Use

   CRISPR

   Tags

   NLS and VP64

   Expression

   Mutation

   nuclease-null

   Promoter

   CMV

   Available since

   Oct. 22, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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2. GAL-SUV39H2-L

   Plasmid

   #89136

   Purpose

   mammalian expression of full-length 410 amino acid SUV39H2 histone methyltransferase linked to GAL4 DNA binding domain

   Depositor

   Elizabeth Wilson

   Article

   Askew et al Mol Cell Endocrinol. 2017 Mar 5;443:42-5

   Insert

   SUV39H2 (SUV39H2 Human)
   

   Use

   Tags

   GAL4 DBD

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 10, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCFJ909

   Plasmid

   #44480

   Depositor

   Erik Jorgensen

   Article

   Frokjaer-Jensen et al Nat Methods. 2014 May;11(5):529-34. doi:

   Insert

   Minimal Mos1, unc-119, MCS
   

   Use

   Multiple cloning site vector

   Tags

   Expression

   Mutation

   Promoter

   Available since

   July 30, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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4. PU6::unc-119_sgRNA

   Plasmid

   #46169

   Purpose

   unc-119 targeting sgRNA

   Depositor

   John Calarco

   Article

   Friedland et al Nat Methods. 2013 Jun 30. doi: 10.1038/n

   Insert

   unc-119 targeting sgRNA (unc-119 Synthetic)
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   C. elegans U6 snRNA pol III promoter

   Available since

   July 1, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAAV-SMVP-Cas9N-U6-gRNA P1

   Plasmid

   #80943

   Purpose

   Expresses Cas9N in mammalian cells; expresses gRNA P1 for Pd-l1 binding.

   Depositor

   George Church

   Article

   Chew et al Nat Methods. 2016 Sep 5. doi: 10.1038/nm

   Insert

   Cas9N
   

   Use

   AAV, CRISPR, and Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 3, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. pSLI-N-sandwich-loxP(K13)

   Plasmid

   #85793

   Purpose

   P. falciparum integration plasmid for N-terminal gene fusions (double 2xFKBP) and placing loxP sites using SLI

   Depositor

   Tobias Spielmann

   Article

   Birnbaum et al Nat Methods. 2017 Mar 13. doi: 10.1038/n

   Insert

   Kelch13
   

   Use

   Cre/Lox; P. falciparum genomic integration

   Tags

   double 2xFKBP, GFP

   Expression

   Mutation

   Promoter

   none

   Available since

   March 15, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. pcDNA3-AKAR2-CR

   Plasmid

   #40255

   Depositor

   Michael Lin

   Article

   Lam et al Nat Methods. 2012 Sep 9. doi: 10.1038/nm

   Insert

   AKAR2
   

   Use

   Tags

   His6 and Xpress

   Expression

   Mammalian

   Mutation

   Promoter

   CMV IE

   Available since

   Oct. 4, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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8. pTE105

   Plasmid

   #59400

   Purpose

   MethyloBrick expression vector, tuf promoter; TcR

   Depositor

   Tobias Erb

   Article

   Schada von Borzyskowski et al ACS Synth Biol. 2014 Aug 8.

   Type

   Empty backbone

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Ptuf

   Available since

   Dec. 30, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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9. AAV-FLEX-CAG-CyRFP1

   Plasmid

   #84357

   Purpose

   Expresses CyRFP1 fluorescent protein in an AAV backbone, FLEX version (Cre dependent)

   Depositor

   Ryohei Yasuda

   Article

   Laviv et al Nat Methods. 2016 Oct 31. doi: 10.1038/n

   Insert

   CyRFP1
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Double FLEXed

   Promoter

   CAG

   Available since

   Nov. 22, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. pLncEXP

    Plasmid

    #64865

    Purpose

    Empty vector for lncRNA expression

    Depositor

    Lei Sun

    Article

    Alvarez-Dominguez et al Cell Metab. 2015 May 5;21(5):764-76. doi

    Type

    Empty backbone

    Use

    Retroviral

    Tags

    Expression

    Mutation

    Promoter

    CMV

    Available since

    May 26, 2015

    Availability

    Academic Institutions and Nonprofits only

11. pCDH_SBP-mCherry-GPI

    Plasmid

    #65299

    Purpose

    synchronize trafficking of mCherry-GPI from the ER (RUSH system)

    Depositor

    Franck Perez

    Article

    Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

    Insert

    GPI anchor
    

    Use

    Lentiviral

    Tags

    IL-2 Signal Sequence and Streptavidin Binding Pro…

    Expression

    Mutation

    Promoter

    CMV

    Available since

    Aug. 3, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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12. PARP9-c020

    Plasmid

    #173097

    Purpose

    Expression of recombinant fusion protein in E. coli

    Depositor

    Lari Lehtiö

    Article

    Sowa et al Cell Rep Methods. 2021 Dec 20;1(8):10012

    Insert

    PARP9 (PARP9 Human)
    

    Use

    Tags

    mCerulean

    Expression

    Bacterial

    Mutation

    N310-N493

    Promoter

    Available since

    Nov. 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pAct:dCas9

    Plasmid

    #78903

    Purpose

    Expresses dCas9 under actin promoter for "Scaffold" CRISPRa in Drosophila cells

    Depositor

    Norbert Perrimon

    Article

    Chavez et al Nat Methods. 2016 May 23. doi: 10.1038/n

    Insert

    dCas9
    

    Use

    CRISPR

    Tags

    Expression

    Insect

    Mutation

    Promoter

    pActin (Drosophila)

    Available since

    Aug. 12, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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14. pGH183_MS2-AIDΔDead-Hygro

    Plasmid

    #85414

    Purpose

    lentiviral expression vector for MS2-AIDΔDead fusion protein with hygromycin B selectable marker

    Depositor

    Michael Bassik

    Article

    Hess et al Nat Methods. 2016 Oct 31. doi: 10.1038/n

    Insert

    MS2-AIDΔDead and Hygromycin resistance
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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15. mEos4b-Lifeact-7

    Plasmid

    #54813

    Purpose

    Localization: Actin, Excitation: 505 / 569, Emission: 516 / 581

    Depositor

    Loren Looger, Michael Davidson

    Article

    Paez-Segala et al Nat Methods. 2015 Jan 12. doi: 10.1038/n

    Type

    Empty backbone

    Use

    Tags

    mEos4b-Lifeact

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 20, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits

16. mMaroon-PAK-mMaroon

    Plasmid

    #84361

    Purpose

    FRET acceptor for Cdc42 donor, flanked with the far-red FP mMaroon

    Depositor

    Ryohei Yasuda

    Article

    Laviv et al Nat Methods. 2016 Oct 31. doi: 10.1038/n

    Insert

    mMaroon-PAK-mMaroon
    

    Use

    Tags

    mMaroon flanking PAK domain on N and C terminus

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 22, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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17. pSLI-2xFKBP-GFP(1463000)

    Plasmid

    #85789

    Purpose

    P. falciparum integration plasmid to fuse endogenous genes with tags using SLI

    Depositor

    Tobias Spielmann

    Article

    Birnbaum et al Nat Methods. 2017 Mar 13. doi: 10.1038/n

    Insert

    PF3D7146300 targeting region, 2xFKBP-GFP-2TA-Neo-R
    

    Use

    P. falciparum integration

    Tags

    2xFKBP, GFP

    Expression

    Mutation

    Promoter

    none

    Available since

    March 15, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pcDNA3.1/Puro-CAG-VSFP-CR

    Plasmid

    #40257

    Depositor

    Michael Lin

    Article

    Lam et al Nat Methods. 2012 Sep 9. doi: 10.1038/nm

    Insert

    VSFP-CR
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Oct. 18, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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19. pEB2-TagRFP-T

    Plasmid

    #103998

    Purpose

    Plasmid encoding TagRFP-T

    Depositor

    Philippe Cluzel

    Article

    Balleza et al Nat Methods. 2018 Jan;15(1):47-51. doi:

    Insert

    TagRFP-T
    

    Use

    Low copy

    Tags

    Expression

    Bacterial

    Mutation

    Mutations relative to eqFP578 (R32G, K42R, K67R, …

    Promoter

    proC

    Available since

    Dec. 27, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. pDS220

    Plasmid

    #34980

    Purpose

    Expresses a low-affinity ePDZ variant fused to mCherry in yeast. Illumination with 440 nm light induces binding to LOVpep.

    Depositor

    Michael Glotzer

    Article

    Strickland et al Nat Methods. 2012 Mar 4. doi: 10.1038/nm

    Insert

    ePDZb–mCherry
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    TEF

    Available since

    March 23, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. gRNA_tdtomato_reporter1_SP-2x

    Plasmid

    #79370

    Purpose

    Assays activity of transcriptional activators fused to SP Cas9

    Depositor

    George Church

    Article

    Kiani et al Nat Methods. 2015 Sep 7. doi: 10.1038/nm

    Insert

    tdtomato
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 8, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pKMV-VioB

    Plasmid

    #65348

    Purpose

    systhesis of vioB gene in the violacein pathway

    Depositor

    Junbiao Dai

    Article

    Guo et al Nucleic Acids Res. 2015 May 8. pii: gkv4

    Insert

    vioB
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 16, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pKMV-VioD

    Plasmid

    #65350

    Purpose

    systhesis of vioD gene in the violacein pathway

    Depositor

    Junbiao Dai

    Article

    Guo et al Nucleic Acids Res. 2015 May 8. pii: gkv4

    Insert

    vioD
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 22, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pPD005 pcDNA Golden Gate

    Plasmid

    #138749

    Purpose

    Vector used to express most COMET components, modified from pcDNA3.1/HygroR(+) for BsaI/BpiI Golden Gate compatibility and to lack HygroR.

    Depositor

    Joshua Leonard

    Article

    Donahue et al Nat Commun. 2020 Feb 7;11(1):779. doi: 1

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pLenti-smURFP-T2A-mCherry

    Plasmid

    #80347

    Purpose

    Lentiviral transfer vector under the CMV promoter that expresses smURFP and mCherry.

    Depositor

    Erik Rodriguez, Roger Tsien

    Article

    Rodriguez et al Nat Methods. 2016 Aug 1. doi: 10.1038/nm

    Inserts

    smURFP
    mCherry
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    CMV

    Available since

    Aug. 15, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. M-ST1n-VP64

    Plasmid

    #48675

    Purpose

    Mammalian ST1-VP64 nuclease-null Cas9 activator expression, human optimized

    Depositor

    George Church

    Article

    Esvelt et al Nat Methods. 2013 Sep 29. doi: 10.1038/n

    Insert

    Cas9-VP64, nuclease-null
    

    Use

    CRISPR

    Tags

    NLS and VP64

    Expression

    Mutation

    nuclease-null (D9A D598A H599A N622A)

    Promoter

    CMV

    Available since

    Oct. 16, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pAAV-SMVP-Cas9N-U6-gRNA P2

    Plasmid

    #80944

    Purpose

    Expresses Cas9N in mammalian cells; expresses gRNA P2 for Pd-l1 binding

    Depositor

    George Church

    Article

    Chew et al Nat Methods. 2016 Sep 5. doi: 10.1038/nm

    Insert

    Cas9N
    

    Use

    AAV, CRISPR, and Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 3, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. Gi-c001

    Plasmid

    #173078

    Purpose

    Expression of recombinant fusion protein in E. coli

    Depositor

    Lari Lehtiö

    Article

    Sowa et al Cell Rep Methods. 2021 Dec 20;1(8):10012

    Insert

    GNAI1 (GNAI1 Human)
    

    Use

    Tags

    mCitrine

    Expression

    Bacterial

    Mutation

    codon optimized

    Promoter

    Available since

    Dec. 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pHRdSV40_scFv_GCN4_sfGFP-VPR-GB1_NLS

    Plasmid

    #79373

    Purpose

    Recruits VPR to a compatible Cas9 protein for transcriptional activation

    Depositor

    George Church

    Article

    Chavez et al Nat Methods. 2016 May 23. doi: 10.1038/n

    Insert

    GCN4-sfGFP-VPR
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 26, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pLifeAct_mScarlet-H_N1

    Plasmid

    #85055

    Purpose

    In vivo visualization of actin cytoskeleton (can be used for colocalization studies)

    Depositor

    Dorus Gadella

    Article

    Bindels et al Nat Methods. 2016 Nov 21. doi: 10.1038/n

    Insert

    LifeAct
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Dec. 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. Str-KDEL_flGalT-SBP-mCherry

    Plasmid

    #65273

    Purpose

    synchronize trafficking of GalT from the ER (RUSH system)

    Depositor

    Franck Perez

    Article

    Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

    Insert

    galactosidase
    

    Use

    Tags

    Streptavidin Binding Protein (SBP) and mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 10, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. CL7-LbCas12aD156R

    Plasmid

    #164659

    Purpose

    Bacterial expression of mutated LbCas12aD156R using CL7/Im7

    Depositor

    Piyush Jain

    Article

    Nguyen et al Methods. 2021 Feb 9. pii: S1046-2023(21)

    Insert

    LbCas12a-D156R
    

    Use

    Tags

    Thioredoxin, CL7, SUMO

    Expression

    Bacterial

    Mutation

    changed Aspartic Acid 156 to Arginine

    Promoter

    T7

    Available since

    April 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pcDNA3.1-GoldenGate-VP64

    Plasmid

    #47389

    Purpose

    Expression plasmid for TALE proteins fused to VP64 activation domain compatible with GoldenGate cloning system

    Depositor

    Charles Gersbach

    Article

    Perez-Pinera et al Nat Methods. 2013 Mar;10(3):239-42. doi:

    Type

    Empty backbone

    Use

    TALEN

    Tags

    FLAG, HA, and SV40 NLS

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 14, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pAC-4CL1

    Plasmid

    #35947

    Depositor

    Claudia Schmidt-dannert

    Article

    Watts et al BMC Biotechnol. 2006 Mar 21;6:22.

    Insert

    4-coumaroyl CoA ligase 1 (4CL1 Mustard Weed)
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    pLac-mod

    Available since

    Sept. 5, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. 3xFLAG-dCas9/pTEF1p-CYC1t

    Plasmid

    #62190

    Purpose

    Expresses 3xFLAG-dCas9 in budding yeast for enChIP analysis to purify specific genomic regions of interest.

    Depositor

    Hodaka Fujii

    Article

    Fujii et al Biol Methods Protoc. 2022 Oct 17;7(1):bp

    Insert

    3xFLAG-dCas9
    

    Use

    CRISPR

    Tags

    3xFLAG and NLS

    Expression

    Yeast

    Mutation

    human codon-optimized, D10A, H840A

    Promoter

    TEF1 promoter

    Available since

    Feb. 24, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. modRNAc0-Cas9

    Plasmid

    #170180

    Purpose

    IVT template plasmid for making modified Cas9 RNA

    Depositor

    Xiaojun Lian

    Article

    Haideri et al Cell Rep Methods. 2022 Sep 7;2(9):100290

    Insert

    Cas9
    

    Use

    Ivt template plasmid

    Tags

    Expression

    Mutation

    Promoter

    T7 promoter

    Available since

    Sept. 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. mMaroon-RTK-mMaroon

    Plasmid

    #84359

    Purpose

    FRET acceptor for RhoA donor, flanked with the far-red FP mMaroon

    Depositor

    Ryohei Yasuda

    Article

    Laviv et al Nat Methods. 2016 Oct 31. doi: 10.1038/n

    Insert

    mMaroon-Rtk--mMaroon
    

    Use

    Tags

    mMaroon flanking Rtk domain on N and C terminus

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 22, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pTE101

    Plasmid

    #59396

    Purpose

    MethyloBrick cloning vector, no promoter; KmR

    Depositor

    Tobias Erb

    Article

    Schada von Borzyskowski et al ACS Synth Biol. 2014 Aug 8.

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    none

    Available since

    Dec. 30, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pSLI-sandwich(0807600)

    Plasmid

    #85790

    Purpose

    P. falciparum integration plasmid to fuse endogenous genes with tags (double 2xFKBP tag) using SLI

    Depositor

    Tobias Spielmann

    Article

    Birnbaum et al Nat Methods. 2017 Mar 13. doi: 10.1038/n

    Insert

    PF3D0807600 targeting region, 2xFKBP-GFP-2xFKBP-2TA-Neo-R
    

    Use

    P. falciparum genomic integration

    Tags

    2xFKBP, GFP

    Expression

    Mutation

    Promoter

    none

    Available since

    March 15, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pHRdSV40_scFv_GCN4_sfGFP_p65-hsf1_GB1_NLS

    Plasmid

    #79372

    Purpose

    Recruits p65-hsf1 to a compatible Cas9 protein for transcriptional activation

    Depositor

    George Church

    Article

    Chavez et al Nat Methods. 2016 May 23. doi: 10.1038/n

    Insert

    GCN4-sfGFP-p65-hsf1
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 6, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge