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pTK372

Plasmid

#114687

Purpose

sgRNA targeting NuMA's C-terminal locus

Depositor

Tomomi Kiyomitsu

Article

Okumura et al Elife. 2018 May 31;7. pii: 36559. doi: 1

Insert

NuMA sgRNA

Available Since

Sept. 28, 2018

Availability

Academic Institutions and Nonprofits only

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pWT065a

Plasmid

#107912

Purpose

W2m.3.1-1 plasmid. Contains (TCF-LEF)7-BE3 and is ppart of the CAMERA2m system for cellular recording in mammalian cells

Depositor

David Liu

Article

Tang et al Science. 2018 Feb 15. pii: science.aap89

Insert

(TCF-LEF)7-BE3

Expression

Mammalian

Available Since

March 29, 2018

Availability

Academic Institutions and Nonprofits only

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pWT046a

Plasmid

#107897

Purpose

W2.2, W2.4-1 plasmid. Contains PTetO-SD8-BE2, PBAD-sgRNA3 and is part of CAMERA 2.2 and 2.4

Depositor

David Liu

Article

Tang et al Science. 2018 Feb 15. pii: science.aap89

Insert

PTetO-SD8-BE2, PBAD-sgRNA3

Expression

Bacterial

Available Since

April 17, 2018

Availability

Academic Institutions and Nonprofits only

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pTK398

Plasmid

#114714

Purpose

mAID-mClover-3FLAG tagging at NuMA's C-terminal locus

Depositor

Tomomi Kiyomitsu

Article

Okumura et al Elife. 2018 May 31;7. pii: 36559. doi: 1

Insert

mAID-mClover-3FLAG

Available Since

Oct. 17, 2018

Availability

Academic Institutions and Nonprofits only

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pJPC12-ΔM13-mCherry-P/PM,5G6G-GFP

Plasmid

#80911

Purpose

Reporter plasmid (mCherry and GFP)

Depositor

Mark Isalan

Article

Brodel et al Nat Commun. 2016 Dec 16;7:13858. doi: 10

Inserts

mCherry
GFP

Expression

Bacterial

Promoter

P5G6G and PM,5G6G

Available Since

Dec. 19, 2016

Availability

Academic Institutions and Nonprofits only

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pWT065c

Plasmid

#107913

Purpose

W2m.3.2-1 plasmid. Contains (TCF-LEF)7-BE3-P2A-Luc and is ppart of the CAMERA2m system for cellular recording in mammalian cells

Depositor

David Liu

Article

Tang et al Science. 2018 Feb 15. pii: science.aap89

Insert

(TCF-LEF)7-BE3-P2A-Luc

Expression

Mammalian

Available Since

March 29, 2018

Availability

Academic Institutions and Nonprofits only

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pTK421

Plasmid

#114688

Purpose

RFP-Nano tagging at NuMA's C-terminal locus

Depositor

Tomomi Kiyomitsu

Article

Okumura et al Elife. 2018 May 31;7. pii: 36559. doi: 1

Insert

RFP-Nano

Available Since

Sept. 28, 2018

Availability

Academic Institutions and Nonprofits only

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pJPC12-ΔM13-mCherry-P/PM,4A5C6G7G-GFP

Plasmid

#80914

Purpose

Reporter plasmid (mCherry and GFP)

Depositor

Mark Isalan

Article

Brodel et al Nat Commun. 2016 Dec 16;7:13858. doi: 10

Inserts

mCherry
GFP

Expression

Bacterial

Promoter

P4A5C6G7G and PM,4A5C6G7G

Available Since

Dec. 19, 2016

Availability

Academic Institutions and Nonprofits only

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pWT062d

Plasmid

#107911

Purpose

W2m.2-4 plasmid. Contains H1-TetR-sgRNA B and is ppart of the CAMERA2m system for cellular recording in mammalian cells

Depositor

David Liu

Article

Tang et al Science. 2018 Feb 15. pii: science.aap89

Insert

H1-TetR-sgRNA B

Expression

Mammalian

Available Since

April 18, 2018

Availability

Academic Institutions and Nonprofits only

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pWT062e

Plasmid

#107910

Purpose

W2m.2-3 plasmid. Contains U6-LacI-sgRNA A and is ppart of the CAMERA2m system for cellular recording in mammalian cells

Depositor

David Liu

Article

Tang et al Science. 2018 Feb 15. pii: science.aap89

Insert

U6-LacI-sgRNA A

Expression

Mammalian

Available Since

April 18, 2018

Availability

Academic Institutions and Nonprofits only

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pWT050d

Plasmid

#107901

Purpose

W2.5-2 plasmid. Contains PLacO-sgRNA1, PRha-sgRNA6-(sgRNA4-C16T-C18T) and is part of CAMERA 2.5

Depositor

David Liu

Article

Tang et al Science. 2018 Feb 15. pii: science.aap89

Insert

PLacO-sgRNA1, PRha-sgRNA6-(sgRNA4-C16T-C18T)

Expression

Bacterial

Available Since

April 17, 2018

Availability

Academic Institutions and Nonprofits only

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pWT046e

Plasmid

#107900

Purpose

W2.5-1 plasmid. Contains PTetO-SD8-BE2, PBAD-sgRNA5 (sgRNA3-A8C) and is part of CAMERA 2.5

Depositor

David Liu

Article

Tang et al Science. 2018 Feb 15. pii: science.aap89

Insert

PTetO-SD8-BE2, PBAD-sgRNA5 (sgRNA3-A8C)

Expression

Bacterial

Available Since

April 17, 2018

Availability

Academic Institutions and Nonprofits only

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pTK600

Plasmid

#114696

Purpose

SNAP-tagging at DHC's C-terminal locus (BSD)

Depositor

Tomomi Kiyomitsu

Article

Okumura et al Elife. 2018 May 31;7. pii: 36559. doi: 1

Insert

SNAP

Available Since

Oct. 16, 2018

Availability

Academic Institutions and Nonprofits only

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pWT060f

Plasmid

#107907

Purpose

W2m.1-2 plasmid. Contains U6-sgRNA D (CCR5) and is ppart of the CAMERA2m system for cellular recording in mammalian cells

Depositor

David Liu

Article

Tang et al Science. 2018 Feb 15. pii: science.aap89

Insert

U6-sgRNA D (CCR5)

Expression

Mammalian

Available Since

March 29, 2018

Availability

Academic Institutions and Nonprofits only

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pJPC12-ΔM13-mCherry-P/PM,4A5T6T-GFP

Plasmid

#80913

Purpose

Reporter plasmid (mCherry and GFP)

Depositor

Mark Isalan

Article

Brodel et al Nat Commun. 2016 Dec 16;7:13858. doi: 10

Inserts

mCherry
GFP

Expression

Bacterial

Promoter

P4A5T6T and PM,4A5T6T

Available Since

Dec. 19, 2016

Availability

Academic Institutions and Nonprofits only

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pTK388

Plasmid

#114713

Purpose

mAID-mClover-3FLAG tagging at DHC's C-terminal locus

Depositor

Tomomi Kiyomitsu

Article

Okumura et al Elife. 2018 May 31;7. pii: 36559. doi: 1

Insert

mAID-mClover-3FLAG

Available Since

Oct. 17, 2018

Availability

Academic Institutions and Nonprofits only

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pTK308

Plasmid

#114689

Purpose

sgRNA targeting DHC's C-terminal locus

Depositor

Tomomi Kiyomitsu

Article

Okumura et al Elife. 2018 May 31;7. pii: 36559. doi: 1

Insert

DHC-C sgRNA

Available Since

Sept. 28, 2018

Availability

Academic Institutions and Nonprofits only

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EKv444(pIhcsrI677-Cas9 GG destination vector )

Plasmid

#248344

Purpose

Golden Gate destination vector containing S. pyogenes Cas9 nuclease (with BsaI sites removed). For multicassette gRNA assembly and expression in moss Physcomitrium patens (Physcomitrella).

Depositor

Elena Kozgunova

Article

Kozgunova et al bioRxiv 2025.07.29.667574

Type

Empty backbone

Use

CRISPR

Expression

Plant

Available Since

Jan. 6, 2026

Availability

Academic Institutions and Nonprofits only

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pSuccessor

Plasmid

#228391

Purpose

intended to be co-transformed along with pDawn-Ag43 to generate successor strain (SS-GFP) for multipattern biofilm lithography

Depositor

Ingmar Riedel-Kruse

Article

Jin et al Nat Commun. 2024 Nov 1;15(1):9443. doi:

Insert

pConst23100-sfGFP__pLambda-DHFR

Use

Synthetic Biology

Available Since

Nov. 20, 2024

Availability

Academic Institutions and Nonprofits only

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pDifferentiation

Plasmid

#228393

Purpose

intended to be co-transformed along with pDawn-Ag43 to generate differentiation strain (DS-mRuby2) for multipattern biofilm lithography

Depositor

Ingmar Riedel-Kruse

Article

Jin et al Nat Commun. 2024 Nov 1;15(1):9443. doi:

Insert

pLambda-luxR+LVAtag__pLux-mRuby2

Use

Synthetic Biology

Available Since

Nov. 20, 2024

Availability

Academic Institutions and Nonprofits only

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