We narrowed to 11,554 results for: Vars;

1. p3xFLAG-MKL1

   Plasmid

   #11978

   Purpose

   For mammalian expression of human MKL1 with a 3x FLAG tag

   Depositor

   Ron Prywes

   Article

   Cen et al Mol Cell Biol. 2003 Sep . 23(18):6597-60

   Insert

   MKL1 (MRTFA Human)
   

   Use

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   N579S T716A

   Promoter

   CMV

   Available Since

   Aug. 10, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCAGGS_VP30_MARV

   Plasmid

   #135472

   Purpose

   Mammalian expression vector encoding Marburg virus VP30 gene under control of the AG promoter

   Depositor

   Elke Mühlberger

   Article

   Manhart et al Cell Rep. 2018 Sep 4;24(10):2573-2580.e4

   Insert

   VP30
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   None

   Promoter

   AG

   Available Since

   July 13, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. Ypet-PBD

   Plasmid

   #105290

   Purpose

   To measure Rac or Cdc42 activity by binding and FRET analysis

   Depositor

   Kenneth Yamada

   Article

   Petrie et al J Cell Biol. 2012 Apr 30;197(3):439-55.

   Insert

   Pak1 (PAK1 Human)
   

   Use

   Tags

   Ypet

   Expression

   Mammalian

   Mutation

   aa encoding 65-150

   Promoter

   Available Since

   Feb. 21, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pDV24_AAV2_DART-VADAR_iRFP720-sensor

   Plasmid

   #200411

   Purpose

   AAV vector encoding a DART-VADAR sensor for iRFP720 mRNA

   Depositor

   James Collins

   Article

   Gayet et al Nat Commun. 2023 Mar 11;14(1):1339. doi:

   Insert

   DART VADAR sensor
   

   Use

   AAV and Synthetic Biology

   Tags

   MCP-ADAR2dd(E488Q)-NES, TagBFP, and mNeonGreen

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   June 30, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pMDJ20 - pEXP [Psmu-1 | Cre Recombinase (PATCs) | smu-1 UTR]

   Plasmid

   #159808

   Purpose

   Optimized enzyme for genome editing in C. elegans

   Depositor

   Christian Froekjaer-Jensen

   Article

   Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

   Insert

   pEXP [Psmu-1 | Cre Recombinase (PATCs) | smu-1 UTR]
   

   Use

   Tags

   Expression

   Worm

   Mutation

   Not applicable

   Promoter

   Available Since

   Dec. 17, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pNW-sfGFP(SPS6)

   Plasmid

   #133814

   Purpose

   Fluorescent protein sfGFP(SPS6) variant for optimized expression in Bacillus mycoides.

   Depositor

   Oscar Kuipers

   Article

   Yi et al Environ Microbiol Rep. 2018 Feb;10(1):57

   Insert

   sfGFP(SPS6)
   

   Use

   E.coli/bacillus spp. shuttle vector

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Ppta

   Available Since

   Nov. 1, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. GFP-noPS

   Plasmid

   #177943

   Purpose

   Transient mammalian expression of eGFP. Lacks packaging sequence and is used as a negative control transfer plasmid for SARS-CoV-2 virus-like particles.

   Depositor

   Jennifer Doudna

   Article

   Syed et al Science. 2021 Nov 4:eabl6184. doi: 10.11

   Insert

   eGFP
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Dec. 15, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. Lenti-UbC-FKRP-EF1a-BSD

   Plasmid

   #205150

   Purpose

   Express UbC-driven FKRP and EF-1a driven BSD

   Depositor

   Monkol Lek

   Insert

   Fukutin-related protein (FKRP Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   UbC

   Available Since

   April 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pENTR-HLA-A2_GFP

   Plasmid

   #85161

   Purpose

   HLA.A2_GFP fusion with 2 glycines in between

   Depositor

   Tone Fredsvik Gregers, Sébastien Walchli

   Article

   Walchli et al Eur J Immunol. 2014 Mar;44(3):774-84. do

   Insert

   HLA.A2 (HLA-A Human)
   

   Use

   Gateway entry vector

   Tags

   EGFP

   Expression

   Mutation

   Promoter

   Available Since

   Jan. 4, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. pIZ-Flag6His-BmAgo3

    Plasmid

    #50559

    Purpose

    Expresses FLAG, His tagged BmAgo3 in silkworm cell line BmN4 or insect cells

    Depositor

    Yukihide Tomari

    Article

    Kawaoka et al RNA. 2009 Jul;15(7):1258-64. doi: 10.126

    Insert

    BmAgo3
    

    Use

    Tags

    6xHIS and Flag

    Expression

    Insect

    Mutation

    Promoter

    OpIE2

    Available Since

    Feb. 6, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAAV-GFAP104-melanopsin-mCherry

    Plasmid

    #122630

    Purpose

    AAV-mediated expression of melanopsin-mCherry under the GFAP promoter

    Depositor

    Edward Boyden

    Article

    Mederos et al Glia. 2019 May;67(5):915-934. doi: 10.10

    Insert

    melanopsin-mCherry
    

    Use

    AAV

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    GFAP104

    Available Since

    May 16, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCMV-SlugABE-NNG-P2A-puro

    Plasmid

    #214354

    Purpose

    Expresses SlugABE-NNG and puromycin resistance genes

    Depositor

    Yongming Wang

    Article

    Qi et al Nat Chem Biol. 2023 Dec 5. doi: 10.1038/

    Insert

    SlugABE-NNG and puromycin resistance genes
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Q782R/S888R/L906R/N984S/E1012K/K1016I

    Promoter

    Available Since

    Feb. 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pQE81L-KOFP7

    Plasmid

    #160330

    Purpose

    This plasmid encodes an engineered oxygen-independent flavin binding fluorescent protein with N-terminal 6xHis tag. Its quantum yield is comparable to EGFP.

    Depositor

    Sang Taek Jung

    Article

    Ko et al J Agric Food Chem. 2020 May 27;68(21):58

    Insert

    KOFP7
    

    Use

    Tags

    6xHis tag

    Expression

    Bacterial

    Mutation

    Promoter

    T5

    Available Since

    March 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCMV-SlugCas9-NNG-P2A-puro

    Plasmid

    #214352

    Purpose

    Expresses SlugCas9-NNG and puromycin resistance genes

    Depositor

    Yongming Wang

    Article

    Qi et al Nat Chem Biol. 2023 Dec 5. doi: 10.1038/

    Insert

    SlugCas9-NNG and puromycin resistance genes
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Q782R/S888R/L906R/N984S/E1012K/K1016I

    Promoter

    Available Since

    Feb. 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pMS_366

    Plasmid

    #182131

    Purpose

    expresses retron RNA, ec.86 Reverse Transcriptase, and CspRecT SSAP, to create a specific rifampicin resistance mutation

    Depositor

    George Church

    Article

    Schubert et al Proc Natl Acad Sci U S A. 2021 May 4;118

    Inserts

    retron RNA
    ec.86 RT
    CspRecT
    

    Use

    Tags

    Expression

    Mutation

    incorporated rifampicin-resistance-conferring don…

    Promoter

    Available Since

    Sept. 1, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pcpBpaRS

    Plasmid

    #196484

    Purpose

    To express benzoylphenylalanine-specific variant of E. coli TyrRS (pBpaRS) and its cognate amber suppressor tRNA to allow UAG codon to be translated into benzoylphenylalanine.

    Depositor

    Kensaku Sakamoto

    Article

    Hino et al Methods Mol Biol. 2012;794:215-28. doi:

    Insert

    benzoylphenylalanine-specific variant of E. coli TyrRS (pBpaRS), amber suppressor tRNA
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV (synthetase), Pol III tRNA promoter (tRNA)

    Available Since

    Jan. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pGEX-parkin

    Plasmid

    #227367

    Purpose

    For bacterial expression of parkin

    Depositor

    Kalle Gehring

    Article

    Huq et al Commun Biol. 2024 Aug 8;7(1):961. doi: 1

    Insert

    Parkin (PRKN Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Codon usage optimized for E.coli expression.

    Promoter

    Available Since

    Nov. 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pKAR2-Br512_Mut235

    Plasmid

    #179278

    Purpose

    Expresses Br512_Mut235 polymerase in E.coli

    Depositor

    Andrew Ellington

    Article

    Paik et al Biochemistry. 2021 Nov 11. doi: 10.1021/

    Insert

    Engineered Bst DNA Polymerase I Large Fragment
    

    Use

    Tags

    8x His tag; Villin Headpiece 47 amino acid (HP47)…

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    May 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pHR_PGK_SNIPR_ALPPL2 Hinge Notch SNIPR

    Plasmid

    #188377

    Purpose

    Lentiviral vector - constitutive expression of an anti-ALPPL2 SNIPR with a CD8alpha-variant2-ECD, a Notch1-TMD, a Notch1-JMD, and a Gal4VP64 transcriptional factor

    Depositor

    Kole Roybal

    Article

    Zhu et al Cell. 2022 Apr 14;185(8):1431-1443.e16.

    Insert

    PGK_antiALPPL2_CD8alpha-variant2-ECD_Notch1-TMD_Notch1-JMD_Gal4VP64
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    March 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. pFRT-PonA-BI-Gl-WT-24xMS2-PTC-18xPP7 (WP)

    Plasmid

    #189792

    Purpose

    Express wild-type and PTC-containing beta-globin genes bi-directionally under ponasteron A inducible promoter in mammalian cells

    Depositor

    Robert Singer

    Article

    Sato et al Nat Commun. 2021 Dec 10;12(1):7203. doi:

    Insert

    beta-globin (HBB Mouse, Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Ponasterone A inducible

    Available Since

    March 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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