We narrowed to 19,699 results for: ACE
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Plasmid#185324PurposeFor the mammalian expression of the human protein ApoE2 attached to SspB for light inducible binding to iLID scaffolds. Systems like this can be used to induce condensate formationDepositorInsertApoE2
UseTagsExpressionMammalianMutationPromoterAvailable sinceJune 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCGT-HCF-1-C600
Plasmid#99364PurposeC-term of HCFC1 proteinDepositorInsertHCF-1 C-term 1436-2035 (HCFC1 Human)
UseTags2x tandem T7 tag and C-mycExpressionMammalianMutationDeletion of HCFC1 cDNAPromoterCMVAvailable sinceSept. 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
pLenti6.2-coHPDL WT 3xFlag-V5
Plasmid#174129PurposeExpresses codon-optimized HPDL with a 3xFlag-V5 tag in mammalian cellsDepositorInsert4-hydroxyphenylpyruvate dioxygenase like (HPDL Human)
UseLentiviralTags3xFLAG-V5ExpressionMammalianMutationCodon optimizedPromoterAvailable sinceSept. 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
deltaVP1 + VP2C-GFP
Plasmid#166676PurposeYeast integrative plasmid for expressing Murine polyomavirus deltaVP1 (GAL1 promoter) and GFP tagged with VP2C (GAL10 promoter). Contains uracil auxotrophic marker (K. lactis URA3)DepositorInsertsVP2C-GFP
Murine polyomavirus deltaVP1 (NLS deletion mutant)
UseSynthetic BiologyTagsExpressionYeastMutationPromoterGAL1 and GAL10Available sinceApril 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
pST_MAHS_RAMVA_81-236-FLAG (pBS0297)
Plasmid#185328PurposeFor the mammalian expression of the tardigrade protein MAHS_RAMVA_81-236-FLAG. This is one of the top performing targets in our apoptosis assay (it protected from apoptosis).DepositorInsertMAHS_RAMVA_81-236-FLAG
UseTagsFLAGExpressionMammalianMutationPromoterAvailable sinceJune 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBabe_puro_DEST_Flag_TLOC1 (220aa)
Plasmid#45273DepositorInsertTLOC1 (SEC62 Human)
UseRetroviralTagsFlagExpressionMammalianMutationPromoterAvailable sinceMay 22, 2013AvailabilityAcademic Institutions and Nonprofits only -
8200 Bicistronic_GFP_ires_hygro
Plasmid#64375PurposeThis a retroviral expression plasmid expressing GFP along with hygro resistance geneDepositorInsertEGFP
UseRetroviralTagsEGFPExpressionMutationPromoterAvailable sinceJuly 3, 2017AvailabilityAcademic Institutions and Nonprofits only -
HTRA3_PDZ_1
Plasmid#103914PurposeProtein expression and purification of HTRA3 PDZ domainDepositorInsertHTRA3 (HTRA3 Human)
UseTagsGSTExpressionBacterialMutationPromotertacAvailable sinceAug. 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCGN-HCF-1-N450
Plasmid#92321PurposeN-term 1-450 of HCF-1 proteinDepositorInsertHCF-1 (HCFC1 Human)
UseTagsHAExpressionMammalianMutationAA 2-450. Note that a SRRASGTYGRV amino-terminal …PromoterCMVAvailable sinceSept. 12, 2017AvailabilityAcademic Institutions and Nonprofits only -
pBabe_puro_DEST_Flag_SLC7A14
Plasmid#45261DepositorInsertSLC7A14 (SLC7A14 Human)
UseRetroviralTagsFlagExpressionMammalianMutationPromoterAvailable sinceMay 23, 2013AvailabilityAcademic Institutions and Nonprofits only -
pNOC_hfnCas12a-Nlux_crRNA NR1
Plasmid#176244PurposepNOC episomal plasmid harboring the humanized fnCas12a gene sequence tagged with Nlux and crRNA targeting the Nitrate reductase gene of N. oceanica IMET1 with spacer 1DepositorInserthumanized fnCas12a
UseCRISPR, Luciferase, and Synthetic Biology ; Expre…TagsluciferaseExpressionMutationPromoterRibosomal subunitAvailable sinceNov. 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLKO-CreERT2 sgRipk4 v3
Plasmid#158048Purposelenti-viral construct with tamoxifen inducible Cre recombinase and U6 driven sgRNA against mouse Ripk4. NO Cas9DepositorInsertRipk4 sgRNA
UseTagsExpressionMammalianMutationPromoterU6Available sinceOct. 5, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pAAV-FLEXfrt-SaCas9-U6-sgGria3
Plasmid#124869PurposeMutagenesis of Gria3DepositorInsertGria3 (Gria3 Mouse)
UseAAV, CRISPR, and Mouse TargetingTagsExpressionMutationPromoterAvailable sinceMay 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
ARB365
Plasmid#124048PurposeEncodes pEF1a driving expression of TET3G P2A iRFP713, pEF1a expressing sadCas9 fused to mRuby2 P2A mRuby2, and an sgRNA targeting pUAS expressing mAzamiGreen in a PiggyBac destination vectorDepositorInsertPB_pEF1a-tet3G-P2A-iRFP713_pEF1a-sadCAS9::mRuby2-P2A-mRuby2-SV40PA_mu6-sgUAS_pUAS-NLS::mAzamiGreen-rgPA
UseTagsExpressionMammalianMutationPromoterAvailable sinceDec. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
PDZK1_PDZ_1
Plasmid#103927PurposeProtein expression and purification of PDZK1 PDZ1 domainDepositorInsertPDZK1 (PDZK1 Human)
UseTagsGSTExpressionBacterialMutationPromotertacAvailable sinceAug. 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
SLC9A3R2_PDZ_2
Plasmid#103926PurposeProtein expression and purification of NHERF2 PDZ2 domainDepositorInsertSLC9A3R2 (NHERF2 Human)
UseTagsGSTExpressionBacterialMutationPromotertacAvailable sinceAug. 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
PDZK1_PDZ_4
Plasmid#103945PurposeProtein expression and purification of PDZK1 PDZ4 domainDepositorInsertPDZK1 (PDZK1 Human)
UseTagsGSTExpressionBacterialMutationPromotertacAvailable sinceAug. 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
delNC C-His Ana GvpC in pET28a
Plasmid#85736PurposeExpresses a truncated His-tagged GvpC from Anabaena flos-aquae without the N-and C-terminal regions in E.coliDepositorInsertGas vesicle protein C (truncated and his-tagged)
UseTagsHis-tagExpressionBacterialMutationDeletion of N and C terminal regions, addition of…PromoterT7Available sinceMarch 29, 2017AvailabilityAcademic Institutions and Nonprofits only -
pENTR1A dn-cHSF1
Plasmid#134724PurposeGateway entry vector for constitutively active dominant-negative HSF1DepositorInsertdn-cHSF1 (HSF1 Human)
UseEntry vector for gateway cloningTagsExpressionMutationDeletion of amino acids 186-202, 379−529PromoterNoneAvailable sinceJuly 5, 2021AvailabilityAcademic Institutions and Nonprofits only -
pTKan-p35S::Csy4-pNOS::cogRFP (C134, JBEI-15901)
Plasmid#110146PurposeTransformation and expression of Csy4 and cogDsRed proteins in plantsDepositorInsertCsy4 (ATCS Mustard Weed)
UseTagscogRFPExpressionBacterial and PlantMutationPromoterAvailable sinceJune 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
pBEST-p70a-UTR1-deGFP Y35TAG-6xHis-T500
Plasmid#92225PurposeExpression of deGFP mutant Y35TAG for ncAA incoporation using the p70a promoter and UTR1DepositorInsertdeGFP Y35TAG
UseSynthetic Biology; Cell free protein synthesis (c…Tags6xHisExpressionBacterialMutationResidue Y35 mutated into TAG (Amber) stop codon f…Promoterp70aAvailable sinceOct. 23, 2017AvailabilityAcademic Institutions and Nonprofits only -
MTK234_055
Plasmid#123920PurposeEncodes the spCas9 gRNA GFP dropout expression cassette (bovine u6 promoter and constant region 2) as a type 234 part to be used in the MTK systemDepositorInsertgRNA-GFPDROPOUT-for Sp Cas9 bu6_20N_cr2
UseTagsExpressionMammalianMutationPromoterAvailable sinceDec. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
TJP2_PDZ_3
Plasmid#103949PurposeProtein expression and purification of X104 PDZ3 domainDepositorInsertTJP2 (TJP2 Human)
UseTagsGSTExpressionBacterialMutationPromotertacAvailable sinceAug. 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
TJP2_PDZ_2
Plasmid#103955PurposeProtein expression and purification of X104 PDZ2 domainDepositorInsertTJP2 (TJP2 Human)
UseTagsGSTExpressionBacterialMutationPromotertacAvailable sinceAug. 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
MTK0_046
Plasmid#123976PurposeEncodes the Cas9 Thal5.10-2_hg18 homology destination with Hygromycin resistance cassette GFP dropout destination vector as a type 0 part to be used in the MTK systemhDepositorInserthThal5.10-2_hg18 CAS9 Destination - HygroR
UseTagsExpressionMammalianMutationPromoterAvailable sinceDec. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
JPF0419v
Plasmid#124021PurposeEncodes pCAG driving expression of multicistronic Lyn-tagged iRFP713, cytoplasmic mAzamiGreen, mCerulean-tethered p38 KTR, and Histone 2B fused to mScarlet in a PiggyBac destination vectorDepositorInsertPB_pCAG-Lyn-iRFP713_pCAG-NES-mAzamiGreen_pCAG-p38KTR-mCerulean_pCAG-H2B::mScarlet
UseTagsExpressionMammalianMutationPromoterAvailable sinceDec. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
pZS1[FruR-T,LacI-T]
Plasmid#60768PurposeContains PIq driving expression FruR-T, and PI driving expression of LacI-T.DepositorInsertsFruR-T
Dimeric LacI with the TAN DBD
UseSynthetic BiologyTagsExpressionBacterialMutationDimeric LacI with the TAN DBD. and LacI/GalR repr…PromoterAvailable sinceJuly 10, 2015AvailabilityAcademic Institutions and Nonprofits only -
pZS1[GalS-T,RbsR-T]
Plasmid#60773PurposeContains PIq driving expression GalS-T, and PIq driving expression of RbsR-T.DepositorInsertsGalS-T
GalS-T
UseSynthetic BiologyTagsExpressionBacterialMutationLacI/GalR repressor chimera. TAN DBD with GalS LB…PromoterAvailable sinceJuly 10, 2015AvailabilityAcademic Institutions and Nonprofits only -
PCRII apoEa
Plasmid#121900PurposePlasmid for making zebrafish apoEa in situ probeDepositorInsertApoEa (apoea Zebrafish)
UseUnspecifiedTagsExpressionMutationPromoterAvailable sinceMay 10, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCSIIneo-TRE-hPhyB621- mCherry-HRasCT
Plasmid#139482PurposeExpresses PhyB621-mCherry-HRasCT in mammalian cells.DepositorInsertPhyB621 (PHYB Mustard Weed)
UseLentiviralTagsmCherry-HRasCTExpressionMammalianMutationG229L and deletion of aa Y at position 235 in mch…PromoterTet responsible elementAvailable sinceApril 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
pTKan-p35S::Csy4-pNOS::cogGFP (C132, JBEI-15898)
Plasmid#110145PurposeTransformation and expression of Csy4 and cogGFP proteins in plantsDepositorInsertCsy4 (ATCS Mustard Weed)
UseTagscogGFPExpressionBacterial and PlantMutationPromoterAvailable sinceJune 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAV10.F6.loxP
Plasmid#63201PurposepCACS backbone (Amp), KlURA3, contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for TU assembly using yGG. LoxP flanked TU. Integration into chrVI: 97873-98803 (NotI or BciVI).DepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionBacterial and YeastMutationPromoterAvailable sinceOct. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
ROZA-XL YF
Plasmid#64195PurposeFluorescent reporter for ZAP-70 tyrosine kinase activity- Y to F mutated control sequenceDepositorInsertROZA-XL YF
UseTagsExpressionMammalianMutationROZA-XL tyrosine phosphorylation site mutated to …PromoterCMVAvailable sinceApril 23, 2015AvailabilityAcademic Institutions and Nonprofits only -
TetO-FUW-Hes3
Plasmid#61535PurposeExpresses Hes3 in mammalian cellsDepositorInsertHes3 (Hes3 Mouse)
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceJan. 16, 2015AvailabilityAcademic Institutions and Nonprofits only -
MPDZ_PDZ_7
Plasmid#103891PurposeProtein expression and purification of Multiple PDZ protein PDZ7 domainDepositorInsertMPDZ (MPDZ Human)
UseTagsGSTExpressionBacterialMutationPromotertacAvailable sinceAug. 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
MPDZ_PDZ_13
Plasmid#103897PurposeProtein expression and purification of Multiple PDZ protein PDZ13 domainDepositorInsertMPDZ (MPDZ Human)
UseTagsGSTExpressionBacterialMutationPromotertacAvailable sinceAug. 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
MPDZ_PDZ_3
Plasmid#103887PurposeProtein expression and purification of Multiple PDZ protein PDZ3 domainDepositorInsertMPDZ (MPDZ Human)
UseTagsGSTExpressionBacterialMutationPromotertacAvailable sinceAug. 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
NC1 pGLUE WTX (1-804)
Plasmid#36956DepositorInsertWTX (AMER1 Human)
UseTagsCBP tag, HA, and streptagExpressionMammalianMutationPromoterCMVAvailable sinceJuly 24, 2012AvailabilityAcademic Institutions and Nonprofits only -
pBabe-Puro-FLAG-Neuroserpin oloop
Plasmid#58262PurposeRetroviral vector for expressing mutated Neuroserpin in mammalian cellsDepositorInsertNeuroserpin (SERPINI1 Human)
UseRetroviralTagsFLAGExpressionMammalianMutationMutant of neuroserpin lacking five residues from …PromoterAvailable sinceAug. 21, 2014AvailabilityAcademic Institutions and Nonprofits only -
pPKm-231
Plasmid#90500PurposepSIN - EF-1alpha - MTS - tFd - P2A - MTS - tFNR, vector encoding for mitochondrial-tagged Thermosynechococcus elongates Ferredoxin (Fd) and Ferredoxin-NADP(+) oxireductase (FNR), under EF-1alpha promoterDepositorInsertmito-tFD and mito-tFNR
UseLentiviralTagsExpressionMutationPromoterAvailable sinceApril 4, 2018AvailabilityAcademic Institutions and Nonprofits only