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Plasmid#116078PurposeExpresses TVMV proteaseDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertTVMVP
ExpressionMammalianAvailable sinceOct. 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
pEB1-wtGFP
Plasmid#103978PurposePlasmid encoding wtGFPDepositorInsertwtGFP
UseLow copyExpressionBacterialPromoterproCAvailable sinceDec. 13, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pMOD_A0103
Plasmid#91000PurposeModule A, Promoter: CmYLCV, Gene: AtCas9, Terminator: HSPDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertAtCas9
UseCRISPRPromoterCmYLCVAvailable sinceJuly 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
pMyBADC
Plasmid#84688PurposeProtein expression in mycobacteriaDepositorTypeEmpty backboneUseBacterial expression vectorTagsHis6ExpressionBacterialPromoterArabinoseAvailable sinceJan. 25, 2017AvailabilityAcademic Institutions and Nonprofits only -
pMZ376
Plasmid#74213PurposeCombined sgRNA/Cas9 vector with KanMX markerDepositorInsertsCas9
rrk1:sgRNA
UseCRISPRExpressionYeastMutationSilent muation of the CspCI sitePromoteradh1 and rrk1Available sinceMarch 31, 2016AvailabilityAcademic Institutions and Nonprofits only -
pDRM166 LKB (Luciferase mKate2 Blast)
Plasmid#183502PurposeExpresses the firefly luciferase gene, the NLS-tagged mKate2 gene, and the blasticidin resistance gene joined by P2A linkers.DepositorInsertsFirefly Luciferase
mKate2
BSD
UseLentiviral and LuciferaseTagsP2A linker and SV40 NLS with GGS linkerExpressionMammalianPromoterMND, MND (off Luc-P2A), and MND (off Luc-P2A-mKat…Available sinceNov. 30, 2023AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pSLPB2B-FKBP12_F36V-CasRx-tagBFP-PGK-Blasticidin
Plasmid#187952PurposeFKBP12 (F36V mutant) degron-tagged CasRx fused with tagBFP, expressed under EF1-alpha promoter in a piggyBac plasmid. Expresses Blasticidin resistance under PGK promoter.DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertFKBP12_F36V-CasRx-tagBFP
UseCRISPR and Synthetic BiologyTagsGGS linker, HA-tag, and NLSExpressionMammalianAvailable sinceOct. 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
pJSC270 - Bacterial expression plasmid for SpCas9 eSpCas9(1.1)-HF1 variant
Plasmid#101217PurposeBacterial expression plasmid for SpCas9 eSpCas9(1.1)-HF1 variantDepositorInsertSpCas9 variant K848A/K1003A/R1060A/N497A/R661A/Q695A/Q926A
Tags10x His, MBP, and TEV siteExpressionBacterialMutationK848A, K1003A, R1060A, N497A, R661A, Q695A and Q9…PromoterT7Available sinceOct. 25, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pEx1-pEF-H2B-mCherry-T2A-rTetR-HDAC4
Plasmid#78349PurposeExpresses rTetR-HDAC4 for gene silencing and the nuclear H2B-mCherry marker in mammalian cells.DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertH2B-mCherry-T2A-rTetR-HDAC4 (HDAC4 Synthetic, Human)
UseSynthetic BiologyTagsrTetRExpressionMammalianPromoterpEF alphaAvailable sinceJune 15, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
Cre-p2a-tdTomato
Plasmid#176837PurposeExpress Cre in mammalian cells and enable FACS sorting Cre expressing cells by tdTomato expressionDepositorInsertCre-P2A-tdTomato
ExpressionMammalianPromoterCAGGSAvailable sinceNov. 2, 2021AvailabilityAcademic Institutions and Nonprofits onlyCited by -
6x-His_A1aY1_GFP
Plasmid#158753PurposeBacterial expression of 6xHis-A1aY1 fused with carboxy terminus GFP for recombinant purificationDepositorInsert6xHistidine_A1aY1_GFP
Tags6x-Histidine tag, GFP, T7 leader sequence, and Th…ExpressionBacterialPromoterT7Available sinceSept. 14, 2020AvailabilityAcademic Institutions and Nonprofits only -
pSBY3_ligD
Plasmid#104624PurposeCRISPR system used to genome editing in Mycobacterium smegmatis, expresses Fn Cpf1 and crRNA targeting ligDDepositorInsertFnCpf1
UseCRISPRExpressionBacterialMutationFnCpf1 codon-optimized C.glutamicumPromoterhsp60Available sinceJune 25, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCR-Zeo
Plasmid#158709PurposeFor cloning and constitutive expression of crRNAs in mycobacteriumDepositorInsertcrRNA
UseCRISPR and Synthetic Biology; Mycobacteria expres…ExpressionBacterialPromoterHsp60Available sinceJan. 15, 2021AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pN-PITCh-HBTH
Plasmid#127883PurposeProvides the repair template with PP2Ac microhomology on each end for PP2Ac N-ter HBTH taggingDepositorInsertGFP-Puro-HBTH
UseCRISPRExpressionMammalianAvailable sinceSept. 24, 2019AvailabilityAcademic Institutions and Nonprofits only -
pDM_T7_HisLacZomega
Plasmid#118815PurposeT7 RNAP-driven expression of N-terminal His-tagged lacZ omega subunit.DepositorInsertlacZ_
TagshistagExpressionBacterialAvailable sinceAug. 8, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pET-21a_2xNLS_LbCpf1_protein_expression
Plasmid#114366PurposeProtein expression plasmid for 2xNLS LbCpf1 in pET-21a backboneDepositorInsert2xNLS_LbCpf1
UseCRISPRTagsNLSExpressionBacterialPromoterT7Available sinceFeb. 15, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pMRE165
Plasmid#118513Purposebroad-host range plasmid to constitutively express fluorescent protein genes in bacteriaDepositorInsertmScarlet-I
ExpressionBacterialAvailable sinceOct. 30, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET-6xHis-(pos15)GFP
Plasmid#89248Purpose[Edit] Expresses GFP with +15 theoretical charge in E.ColiDepositorInsertHis6-(pos15)-GFP
TagsHis6ExpressionBacterialPromoterT7Available sinceApril 21, 2017AvailabilityAcademic Institutions and Nonprofits only -
MSCV Puro-pGK:GFP
Plasmid#68486PurposeRetrovirus expressing GFP driven by pGK promoterDepositorInsertsPuromycin Resistance
GFP
UseRetroviralExpressionMammalianPromoterLTR and pGKAvailable sinceFeb. 22, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
wtBxb1_IVT
Plasmid#222340PurposePlasmid for wild type Bxb1 mRNA in vitro transcription templateDepositorInsertCodon-optimized wtBxb1
UseTemplate for in vitro transcriptionPromoterT7 (inactivated)Available sinceJune 27, 2024AvailabilityAcademic Institutions and Nonprofits only