We narrowed to 26,523 results for: LEA

1. NCH1ec

   Plasmid

   #131016

   Purpose

   Expression of plant LINC fluorescent fusion protein for cell biology

   Depositor

   Hank Bass

   Article

   McKenna et al Front Plant Sci. 2021 Feb 17;12:645218.

   Insert

   NCH1
   

   Use

   Gateway entry vector

   Tags

   eGFP-FLAG-HA

   Available Since

   May 6, 2022

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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2. NCH1exp

   Plasmid

   #131017

   Purpose

   Expression of plant LINC fluorescent fusion protein for cell biology

   Depositor

   Hank Bass

   Article

   McKenna et al Front Plant Sci. 2021 Feb 17;12:645218.

   Insert

   NCH1
   

   Tags

   eGFP-FLAG-HA

   Expression

   Plant

   Promoter

   P35S

   Available Since

   May 6, 2022

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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3. NCH2ec

   Plasmid

   #131018

   Purpose

   Expression of plant LINC fluorescent fusion protein for cell biology

   Depositor

   Hank Bass

   Article

   McKenna et al Front Plant Sci. 2021 Feb 17;12:645218.

   Insert

   NCH2
   

   Use

   Gateway entry vector

   Tags

   eGFP-FLAG-HA

   Available Since

   May 6, 2022

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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4. pAc5.1B-lambdaN-HA-DmPAN3-dsRNAres_Y

   Plasmid

   #148080

   Purpose

   Insect Expression of DmPAN3-dsRNAres

   Depositor

   Elisa Izaurralde

   Article

   Jonas et al Nat Struct Mol Biol. 2014 Jul;21(7):599-

   Insert

   DmPAN3-dsRNAres (CG11486 Fly)
   

   Expression

   Insect

   Available Since

   March 25, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAc5.1B-lambdaN-HA-DmGW182-delUBAdelQrich_G

   Plasmid

   #146396

   Purpose

   Insect Expression of DmGW182-delUBAdelQrich

   Depositor

   Elisa Izaurralde

   Article

   Zekri et al Mol Cell Biol. 2009 Dec;29(23):6220-31.

   Insert

   DmGW182-delUBAdelQrich (gw Fly)
   

   Expression

   Insect

   Available Since

   March 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pUC19-SpRYgest_targetplasmid (KAC833)

   Plasmid

   #181748

   Purpose

   pUC19 derivative plasmid used as a substrate for SpRYgest, restriction enzyme, and TtAgo digests. Encodes an SpCas9 EMX1-site1-NGG-PAM target site between NheI and HindIII restriction sites.

   Depositor

   Benjamin Kleinstiver

   Article

   Christie et al Nat Biotechnol. 2022 Oct 6. pii: 10.1038

   Type

   Empty backbone

   Use

   Substrate plasmid

   Available Since

   Feb. 16, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLV Pigb-V5

   Plasmid

   #175160

   Purpose

   Lentiviral expression of mouse Pigb-V5

   Depositor

   Larry Gerace

   Article

   Cheng et al Life Sci Alliance. 2023 Jul 11;6(9):e202

   Insert

   Pigb (Pigb Mouse)
   

   Use

   Lentiviral

   Tags

   V5

   Expression

   Mammalian

   Available Since

   Feb. 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pBS-tRNA-Gly

   Plasmid

   #174317

   Purpose

   For in vitro transcription of mouse tRNAs

   Depositor

   Bruce Beutler

   Article

   Yue et al Science. 2021 May 14;372(6543). pii: 372

   Insert

   tRNA-gly
   

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Nov. 23, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. IBMc397

   Plasmid

   #161955

   Purpose

   Demonstrates chemically induced dimmerization of acVHH. Expression plasmid for split, evolved T7 RNA polymerase C-lobe fused to acVHH

   Depositor

   Baojun Wang

   Article

   Ho et al Nat Commun. 2021 Apr 13;12(1):2200. doi:

   Insert

   pSB3K3-TT-PBAD(SapI-)-B30-His-acVHH-15aa-T7RNAP(181+)-T
   

   Expression

   Bacterial

   Promoter

   PBAD

   Available Since

   Oct. 22, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCMV-FLAG-PGRMC-Hybrid1

    Plasmid

    #173469

    Purpose

    N-terminal region of PGRMC2 fused to the transmembrane and C-terminal region of PGRMC1

    Depositor

    Bill Tsai

    Article

    Chen et al Nat Commun. 2021 Oct 13;12(1):5991. doi:

    Insert

    PGRMC1/PGRMC2 fusion (PGRMC1, PGRMC2 Human)
    

    Expression

    Mammalian

    Available Since

    Aug. 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCMV-FLAG-PGRMC-Hybrid2

    Plasmid

    #173470

    Purpose

    N-terminal and transmembrane region of PGRMC2 fused to the C-terminal region of PGRMC1

    Depositor

    Bill Tsai

    Article

    Chen et al Nat Commun. 2021 Oct 13;12(1):5991. doi:

    Insert

    PGRMC1/PGRMC2 fusion (PGRMC1, PGRMC2 Human)
    

    Tags

    FLAG

    Expression

    Mammalian

    Available Since

    Aug. 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCMV-FLAG-PGRMC-Hybrid3

    Plasmid

    #173471

    Purpose

    N-terminal region of PGRMC1 fused to the transmembrane and C-terminal region of PGRMC2

    Depositor

    Bill Tsai

    Article

    Chen et al Nat Commun. 2021 Oct 13;12(1):5991. doi:

    Insert

    PGRMC1/PGRMC2 fusion (PGRMC1, PGRMC2 Human)
    

    Tags

    FLAG

    Expression

    Mammalian

    Available Since

    Aug. 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pET21-pelB-LaG-43

    Plasmid

    #172759

    Purpose

    Bacterial expression of HIS-tagged anti-GFP nanobody LaG-43; HIS tag preceded by HRV 3C/PreScission cleavage site.

    Depositor

    Michael Rout

    Article

    Fridy et al Nat Methods. 2014 Dec;11(12):1253-60. do

    Insert

    LaG-43
    

    Tags

    6xHIS and pelB leader for periplasmic secretion

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Aug. 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pET21-pelB-LaG-35

    Plasmid

    #172755

    Purpose

    Bacterial expression of HIS-tagged anti-GFP nanobody LaG-35; HIS tag preceded by HRV 3C/PreScission cleavage site.

    Depositor

    Michael Rout

    Article

    Fridy et al Nat Methods. 2014 Dec;11(12):1253-60. do

    Insert

    LaG-35
    

    Tags

    6xHIS and pelB leader for periplasmic secretion

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Aug. 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pET21-pelB-LaG-19

    Plasmid

    #172748

    Purpose

    Bacterial expression of HIS-tagged anti-GFP nanobody LaG-19; HIS tag preceded by HRV 3C/PreScission cleavage site.

    Depositor

    Michael Rout

    Article

    Fridy et al Nat Methods. 2014 Dec;11(12):1253-60. do

    Insert

    LaG-19
    

    Tags

    6xHIS and pelB leader for periplasmic secretion

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Aug. 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pET21-pelB-LaG-3

    Plasmid

    #172740

    Purpose

    Bacterial expression of HIS-tagged anti-GFP nanobody LaG-3; HIS tag preceded by HRV 3C/PreScission cleavage site.

    Depositor

    Michael Rout

    Article

    Fridy et al Nat Methods. 2014 Dec;11(12):1253-60. do

    Insert

    LaG-3
    

    Tags

    6xHIS and pelB leader for periplasmic secretion

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Aug. 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pET21-pelB-LaG-12

    Plasmid

    #172744

    Purpose

    Bacterial expression of HIS-tagged anti-GFP nanobody LaG-12; HIS tag preceded by HRV 3C/PreScission cleavage site.

    Depositor

    Michael Rout

    Article

    Fridy et al Nat Methods. 2014 Dec;11(12):1253-60. do

    Insert

    LaG-12
    

    Tags

    6xHIS and pelB leader for periplasmic secretion

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Aug. 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pET21-pelB-LaG-14

    Plasmid

    #172745

    Purpose

    Bacterial expression of HIS-tagged anti-GFP nanobody LaG-14; HIS tag preceded by HRV 3C/PreScission cleavage site.

    Depositor

    Michael Rout

    Article

    Fridy et al Nat Methods. 2014 Dec;11(12):1253-60. do

    Insert

    LaG-14
    

    Tags

    6xHIS and pelB leader for periplasmic secretion

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Aug. 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pCI-Flag-NEDD4L∆WW4

    Plasmid

    #166710

    Purpose

    expresses NEDD4L with inactivating mutation in fourth WW domain in mammalian cells

    Depositor

    Wesley Sundquist

    Article

    Rheinemann et al J Biol Chem. 2021 Jul 17:100975. doi: 10

    Insert

    NEDD4L W576A
    

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    changed tryptophan 576 to alanine

    Promoter

    CMV promoter

    Available Since

    July 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pGEX-PP-NEDD4L-WW1(193-226)

    Plasmid

    #166701

    Purpose

    expresses NEDD4L WW1 in bacterial cells

    Depositor

    Wesley Sundquist

    Article

    Rheinemann et al J Biol Chem. 2021 Jul 17:100975. doi: 10

    Insert

    NEDD4L WW1 domain
    

    Tags

    GST, HRV 3C site, and Thrombin site

    Expression

    Bacterial

    Promoter

    tac promoter

    Available Since

    July 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pGEX-PP-NEDD4L-WW2(385-418)

    Plasmid

    #166702

    Purpose

    expresses NEDD4L WW2 in bacterial cells

    Depositor

    Wesley Sundquist

    Article

    Rheinemann et al J Biol Chem. 2021 Jul 17:100975. doi: 10

    Insert

    NEDD4L WW2 domain
    

    Tags

    GST, HRV 3C site, and Thrombin site

    Expression

    Bacterial

    Promoter

    tac promoter

    Available Since

    July 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pGEX-PP-NEDD4L-WW3(497-530)

    Plasmid

    #166703

    Purpose

    expresses NEDD4L WW3 in bacterial cells

    Depositor

    Wesley Sundquist

    Article

    Rheinemann et al J Biol Chem. 2021 Jul 17:100975. doi: 10

    Insert

    NEDD4L WW3 domain
    

    Tags

    GST, HRV 3C site, and Thrombin site

    Expression

    Bacterial

    Promoter

    tac promoter

    Available Since

    July 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pGEX-PP-NEDD4L-WW4(548-581)

    Plasmid

    #166704

    Purpose

    expresses NEDD4L WW4 in bacterial cells

    Depositor

    Wesley Sundquist

    Article

    Rheinemann et al J Biol Chem. 2021 Jul 17:100975. doi: 10

    Insert

    NEDD4L WW4 domain
    

    Tags

    GST, HRV 3C site, and Thrombin site

    Expression

    Bacterial

    Promoter

    tac promoter

    Available Since

    July 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pCI-Flag-NEDD4L∆WW1

    Plasmid

    #166707

    Purpose

    expresses NEDD4L with inactivating mutation in first WW domain in mammalian cells

    Depositor

    Wesley Sundquist

    Article

    Rheinemann et al J Biol Chem. 2021 Jul 17:100975. doi: 10

    Insert

    NEDD4L W221A
    

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    changed tryptophan 221 to alanine

    Promoter

    CMV promoter

    Available Since

    July 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pET28a(+)-HisTim12_CtoA

    Plasmid

    #170281

    Purpose

    Expresses yeast Tim12 (the latter with cleavable N-ter His-tag), codon-optimized for E. coli production

    Depositor

    Paul Schanda

    Article

    Weinhaupl et al Structure. 2021 May 3. pii: S0969-2126(2

    Insert

    yeast Tim12 (TIM12 Budding Yeast)
    

    Tags

    TEV-cleavable N-terminal His tag (on backbone)

    Expression

    Bacterial

    Mutation

    mutated two non-conserved Cys to Ala

    Available Since

    June 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. IBMc180

    Plasmid

    #161942

    Purpose

    Carries Golden Gate substitution insert for acVHH-assisted bisection mapping

    Depositor

    Baojun Wang

    Article

    Ho et al Nat Commun. 2021 Apr 13;12(1):2200. doi:

    Insert

    pSB1A3-BbsI-10aa-acVHH-T-cat-PBAD(SapI)-RiboJ-BCD22-acVHH-10aa-SapI
    

    Expression

    Bacterial

    Promoter

    PBAD

    Available Since

    March 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. IBMc226

    Plasmid

    #161944

    Purpose

    Contains Type IIS restriction site free elements for expressing protein to be split in IBM. TetR expression plasmid

    Depositor

    Baojun Wang

    Article

    Ho et al Nat Commun. 2021 Apr 13;12(1):2200. doi:

    Insert

    pSB3A5*(BsaI-)-PBAD(SapI-)-tet1-TetR-Ter
    

    Expression

    Bacterial

    Promoter

    PBAD

    Available Since

    March 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pCbh-SpyCas9-T2A-puro-pA_U6-sgPolb (pE047)

    Plasmid

    #149350

    Purpose

    Mammalian expression vector for SpyCas9, puromycin resistance and human Polb guide RNA

    Depositor

    Ralf Kuehn

    Article

    Danner et al Open Biol. 2021 Jan;11(1):200283. doi: 1

    Insert

    SpyCas9
    

    Use

    CRISPR

    Tags

    Flag tag

    Expression

    Mammalian

    Promoter

    Cbh

    Available Since

    Jan. 27, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pMKO.1-shSpc25

    Plasmid

    #160960

    Purpose

    Spc25 shRNA in pMKO.1 retroviral vector

    Depositor

    Matthew Steinhauser

    Article

    Zhang et al J Clin Invest. 2018 Nov 1;128(11):4898-4

    Insert

    Spc25 shRNA (Spc25 Mouse)
    

    Use

    RNAi and Retroviral

    Promoter

    U6

    Available Since

    Dec. 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pAAV-5U6-sgRNAs-hsyn-EGFP

    Plasmid

    #112213

    Purpose

    Targeted DNA methylation

    Depositor

    Xingxu Huang

    Article

    Lu et al Cell Death Dis. 2020 Feb 3;11(2):85. doi

    Insert

    sgRNAs
    

    Use

    AAV

    Available Since

    Oct. 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pYPS1-Venus

    Plasmid

    #133118

    Purpose

    Plasmid contains a reporter gene for Crz1. The 1000bp promoter of the gene pYPS1 driving a Venus.

    Depositor

    Hana El-Samad

    Article

    Chen et al Cell Syst. 2020 Sep 4. pii: S2405-4712(2

    Insert

    pYPS1-YFP
    

    Expression

    Bacterial and Yeast

    Available Since

    Sept. 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pCMK2-Venus

    Plasmid

    #133119

    Purpose

    Plasmid contains a reporter gene for Crz1. The 1000bp promoter of the gene pCMK2 driving a Venus.

    Depositor

    Hana El-Samad

    Article

    Chen et al Cell Syst. 2020 Sep 4. pii: S2405-4712(2

    Insert

    pCMK2-YFP
    

    Expression

    Bacterial and Yeast

    Available Since

    Sept. 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pGYP7-Venus

    Plasmid

    #133120

    Purpose

    Plasmid contains a reporter gene for Crz1. The 1000bp promoter of the gene pGYP7 driving a Venus.

    Depositor

    Hana El-Samad

    Article

    Chen et al Cell Syst. 2020 Sep 4. pii: S2405-4712(2

    Insert

    pGYP7-YFP
    

    Expression

    Bacterial and Yeast

    Available Since

    Sept. 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pET15-His-∆NGlpG(Y205F)

    Plasmid

    #155132

    Purpose

    Expresses N-terminally His-tagged E. coli GlpG Y205F variant (residues 87-276) from pET15b

    Depositor

    Sinisa Urban

    Article

    Cho et al Nat Struct Mol Biol. 2019 Oct;26(10):910

    Insert

    GlpG (glpG Escherichia coli)
    

    Tags

    His

    Expression

    Bacterial

    Mutation

    Y205F, insert codes for GlpG residues 87-276

    Promoter

    T7

    Available Since

    Sept. 8, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pFloat.assemble(AB).LC_v1

    Plasmid

    #133083

    Purpose

    Tier 1 destination vector for assembling gene cassettes intended for expression in bacteria. AB sites. Low copy. Version 1.0.

    Depositor

    Kacper Rogala, David Sabatini

    Article

    Rogala et al Science. 2019 Oct 25;366(6464):468-475.

    Type

    Empty backbone

    Use

    Destination vector

    Expression

    Bacterial

    Available Since

    May 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pFloat.assemble(AB).HC_v1

    Plasmid

    #133084

    Purpose

    Tier 1 destination vector for assembling gene cassettes intended for expression in bacteria. AB sites. High copy. Version 1.0.

    Depositor

    Kacper Rogala, David Sabatini

    Article

    Rogala et al Science. 2019 Oct 25;366(6464):468-475.

    Type

    Empty backbone

    Use

    Destination vector

    Expression

    Bacterial

    Available Since

    May 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pEPOR1CB0015

    Plasmid

    #117551

    Purpose

    Level 1 Golden Gate Cassette: SaCas9 expression cassette for dicotyledonous plants

    Depositor

    Nicola Patron

    Article

    Raitskin et al PLoS One. 2019 Feb 27;14(2):e0211598. do

    Insert

    2x35S+5'UTR OMEGA (pICH51288) + SaCas9 (no stop codon) (pEPOR0SP0012) + YFP (pICSL50005) + Nos (pICH41421)
    

    Expression

    Plant

    Promoter

    2x35S+5'UTR OMEGA (pICH51288)

    Available Since

    Sept. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pEPOR2KN0078

    Plasmid

    #117645

    Purpose

    Level2 MoClo construct, containing level1 plant expression cassettes for eSaCas9(pEPOR1CB0113), sgRNA{AT1G73460}(pEPOR1CB0099) and sgRNA{AT1G73460}(pEPOR1CB0110) to Protein kinase superfamily protein.

    Depositor

    Nicola Patron

    Article

    Raitskin et al PLoS One. 2019 Feb 27;14(2):e0211598. do

    Insert

    [35S:eSaCas9(pEPOR1CB0113) ] +[AtU6-26:sgRNA]
    

    Expression

    Plant

    Available Since

    April 1, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pET41a iGluSnFR E25R

    Plasmid

    #119831

    Purpose

    Production of recombinant iGluSnFR E25R variant, a single-wavelength glutamate indicator, in E. coli

    Depositor

    Katalin Torok

    Article

    Helassa et al Proc Natl Acad Sci U S A. 2018 May 7. pi

    Insert

    iGluSnFR E25R
    

    Tags

    GST

    Expression

    Bacterial

    Mutation

    E25R

    Promoter

    T7

    Available Since

    March 12, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pNOC-CRISPR-compact

    Plasmid

    #99369

    Purpose

    Expresses Cas9-Nlux and sgRNA. Contains CEN/ARS. No selection marker.

    Depositor

    Eva Farre

    Article

    Poliner et al ACS Synth Biol. 2018 Apr 20;7(4):962-968

    Type

    Empty backbone

    Use

    Algae, nannochloropsis expression

    Tags

    Cas9-Nlux

    Promoter

    Ribi promoter

    Available Since

    Feb. 11, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

41. gamma2 UTR shRNA (shRNA4)

    Plasmid

    #120796

    Purpose

    shRNA targeting the 3'-untranslated region (UTR) of the gamma2 mRNA

    Depositor

    Angel de Blas

    Article

    Li et al J Neurochem. 2005 Nov;95(3):756-70. doi:

    Insert

    GABRG2 shRNA (Gabrg2 Rat)
    

    Use

    RNAi

    Available Since

    Jan. 22, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

42. gamma2 CR3m shRNA

    Plasmid

    #120795

    Purpose

    Negative control shRNA for plasmid 120794: shRNA targeting the coding region of the gamma2 mRNA

    Depositor

    Angel de Blas

    Article

    Li et al J Neurochem. 2005 Nov;95(3):756-70. doi:

    Insert

    GABRG2 shRNA (Gabrg2 Rat)
    

    Use

    RNAi

    Available Since

    Jan. 22, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

43. gamma2 UTR3m shRNA

    Plasmid

    #120797

    Purpose

    Negative control shRNA for plasmid 120796: shRNA targeting the 3'-untranslated region (UTR) of the gamma2 mRNA

    Depositor

    Angel de Blas

    Article

    Li et al J Neurochem. 2005 Nov;95(3):756-70. doi:

    Insert

    GABRG2 shRNA (Gabrg2 Rat)
    

    Use

    RNAi

    Available Since

    Jan. 22, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

44. pOCC17

    Plasmid

    #118875

    Purpose

    shuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with N-terminal HIS6 tag, cleavable with 3C, and C-terminal tagRFP

    Depositor

    PEPC MPI CBG, Marc Bickle

    Article

    Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do

    Insert

    NcoI-HIS6-3C--NotI-ccdB-AscI-tagRFP-stop-HindIII cassette
    

    Tags

    HIS6, cleavable with 3C protease and tagRFP

    Expression

    Insect

    Promoter

    polH

    Available Since

    Jan. 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

45. pOCC16

    Plasmid

    #118870

    Purpose

    shuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with N-terminal HIS6 tag, cleavable with 3C, and C-terminal EGFP

    Depositor

    PEPC MPI CBG, Marc Bickle

    Article

    Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do

    Insert

    NcoI-HIS6-3C--NotI-ccdB-AscI-EGFP-stop-HindIII cassette
    

    Tags

    EGFP and HIS6, cleavable with 3C protease

    Expression

    Insect

    Promoter

    polH

    Available Since

    Jan. 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

46. pOCC218

    Plasmid

    #118907

    Purpose

    shuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with N-terminal HIS10, cleavable with 3C protease and N-terminal YBBR tag

    Depositor

    PEPC MPI CBG, Marc Bickle

    Article

    Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do

    Insert

    NcoI-HIS10-3C-YBBR-NotI-ccdB-AscI-stop-HindIII cassette
    

    Tags

    HIS10, cleavable with 3C protease, YBBR tag

    Expression

    Insect

    Promoter

    polH

    Available Since

    Jan. 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

47. pOCC18

    Plasmid

    #118868

    Purpose

    shuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with N-terminal HIS6 tag, cleavable with 3C, and N-terminal tagRFP

    Depositor

    PEPC MPI CBG, Marc Bickle

    Article

    Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do

    Insert

    NcoI-HIS6-3C-tagRFP-NotI-ccdB-AscI-stop-HindIII cassette
    

    Tags

    HIS6, cleavable with 3C protease, tagRFP

    Expression

    Insect

    Promoter

    polH

    Available Since

    Jan. 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

48. pOCC171

    Plasmid

    #118888

    Purpose

    shuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with N-terminal HIS6-MBP tag, cleavable with 3C, and N-terminal SNAP tag

    Depositor

    PEPC MPI CBG, Marc Bickle

    Article

    Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do

    Insert

    NcoI-HIS6-MBP-3C-SNAP-NotI-ccdB-AscI-stop-HindIII cassette
    

    Tags

    HIS6-MBP, cleavable with 3C protease; SNAP tag

    Expression

    Insect

    Promoter

    polH

    Available Since

    Jan. 2, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

49. pOCC202

    Plasmid

    #118880

    Purpose

    shuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with C-terminal mKate2, and C-terminal HIS6 tag, cleavable with 3C

    Depositor

    PEPC MPI CBG, Marc Bickle

    Article

    Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do

    Insert

    NcoI-NotI-ccdB-AscI-mKate2-3C-HIS6-stop-HindIII cassette
    

    Tags

    mKate2, HIS6, cleavable with 3C protease

    Expression

    Insect

    Promoter

    polH

    Available Since

    Jan. 2, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

50. pOCC139

    Plasmid

    #118872

    Purpose

    shuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with N-terminal HIS6 tag, cleavable with 3C, and C-terminal monomeric GFP

    Depositor

    PEPC MPI CBG, Marc Bickle

    Article

    Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do

    Insert

    NcoI-HIS6-3C--NotI-ccdB-AscI-mGFP-stop-HindIII cassette
    

    Tags

    HIS6, cleavable with 3C protease and mGFP

    Expression

    Insect

    Promoter

    polH

    Available Since

    Jan. 2, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge