We narrowed to 8,869 results for: aav
-
Plasmid#89706PurposeDonor plasmid for HIV-1 env- integration into human AAVS1 locusDepositorInsertAAVS1-delta8.2R
UseUnspecifiedPromoterCMVAvailable SinceNov. 3, 2017AvailabilityAcademic Institutions and Nonprofits only -
pET22b-EC12WTC9AAvi
Plasmid#47925PurposeExpresses E-cadherin EC12 WT with C9A mutation and avi tag in E.Coli.DepositorInsertE-Cadherin EC12
Tags6xHis, Avi Tag, and TEV CleavageExpressionBacterialMutationchanged Cysteine 9 to AlaninePromoterT7Available SinceSept. 27, 2013AvailabilityAcademic Institutions and Nonprofits only -
EF1α-TetOn3G_2x cHS4_pTRE3G-loxP-EGFP-lox2272 [AAVS1]
Plasmid#227246PurposeAll-in-one ROSE LP donor construct for TetOn3G-inducible cell line development in HEK293 cellDepositorInsertsTet-On 3G transactivator protein
Enhanced green fluorescent protein
UseCre/Lox and Synthetic BiologyExpressionMammalianPromoterEF1α and pTRE3GAvailable SinceOct. 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAAVS1-Neo-CAG-M2rtTA-H2BmNeon (donorAB-mNeon)
Plasmid#85799PurposedonorAB-Neon targeting vector for human AAVS1 locus; knock-in of Dox controlled H2BGFPDepositorInsertPPP1R12C (PPP1R12C Human)
UseHuman targetingMutationhomology arms for knock-in into first intronAvailable SinceApril 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
CROP_C9_Puro_AAVS1B
Plasmid#183338PurposeAll-in-One CRISPRko system with a guide RNA that targets the AAVS1 locusDepositorInsertAAVS1B
UseLentiviralAvailable SinceMay 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
CROP_C9_Puro_AAVS1A
Plasmid#183337PurposeAll-in-One CRISPRko system with a guide RNA that targets the AAVS1 locusDepositorInsertAAVS1A
UseLentiviralAvailable SinceMay 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLCKO_AAVS1_sgRNA_2
Plasmid#155085Purposelentiviral plasmid expressing Cas9 gRNA targeting AAVS1 safe harbor locusDepositorInsertAAVS1_sgRNA_2
UseCRISPR and LentiviralExpressionMammalianPromoterU6 promoterAvailable SinceAug. 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
pDECKO_mCherry_AAVS1
Plasmid#141212PurposepDECKO_mCherry_AAVS1_186. Targeting, non-essential locus controlDepositorInsertgRNA
UseLentiviralExpressionMammalianAvailable SinceJuly 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGL3- sgAAVS1.5-CAG-Cas9-T2A-mCherry-P2A-Puro
Plasmid#216246PurposeExpress Cas9 with CAG promoter to improve the expression in hES cells and a highly efficient sgAAVS1.5 for AAVS1 integrationDepositorInsertCas9 and sgAAVS1.5
UseCRISPR; Human safe harbor locus (a avs1) site-spe…ExpressionMammalianPromoterCAGAvailable SinceMarch 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
AAVS1-Puro CAG CLTX-NKG2D-2B4-CD3z CAR
Plasmid#157744Purposeknock CLTX-NKG2D-2B4-CD3z CAR into AAVS1 safe harbor locus for constitutive expressionDepositorInsertchlorotoxin containing chimeric antigen receptor targeting glioblastoma
UseCRISPR, Synthetic Biology, and TALENExpressionMammalianPromoterCAGAvailable SinceJan. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
AAVS1-iPEmax-Hygro
Plasmid#214020Purposeinducible PEmax system for controllable prime editing; this plasmid is used to insert PEmax prime editor in one allele of AAVS1 locusDepositorInsertPEmax
ExpressionMammalianPromoterTRE-tightAvailable SinceJune 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
Syn-ChR-tdTomato
Plasmid#99843PurposeAAV expression of ChR2(H134R)-tdTomato under the Syn promoterDepositorInsertChR2(H134R)-tdTomato
UseAAVTagstdTomatoExpressionMammalianMutationChR2(H134R), carrying a single point mutation at …Promoterhuman synapsin I promoterAvailable SinceJan. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
AAVS1-idCas9-KRAB-Hygro donor
Plasmid#199621PurposeDonor vector for genomic targeting of a Tetracycline-inducible dCas9-KRAB cassette to the human AAVS1/PPP1R12C locusDepositorInsertAAVS1-idCas9-KRAB-Hygro
Available SinceApril 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
AAVS1-Puro CAG CLTX-CAR
Plasmid#157743Purposeknock CLTX-CAR into AAVS1 safe harbor locus for constitutive expressionDepositorInsertchlorotoxin containing chimeric antigen receptor targeting glioblastoma
UseCRISPR, Synthetic Biology, and TALENExpressionMammalianPromoterCAGAvailable SinceJan. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
AAVS1-Puro-CAG-fl-STOP-fl-ZEB2-GFP-Flag-WPRE-poly(A)
Plasmid#165456PurposeInducible ZEB2 expression from AAVS1 locusDepositorAvailable SinceJune 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
TR-GFP
Plasmid#80591PurposeExpresses GFP from the CBA promoter. Cloned into an Adeno-Associated Virus backbone for packaging into AAV.DepositorInsertGFP
UseAAVExpressionMammalianPromoterCBAAvailable SinceSept. 22, 2018AvailabilityAcademic Institutions and Nonprofits only -
qTAG-AAVS1-PGK-Puro
Plasmid#227269PurposeEmpty AAVS1 targeting donor for the insertion of Puro and a medium strength PGK promoter. A CDS can be cloned adjacent to the promoter via restriction or gibson cloning using the MluI cut site.DepositorTypeEmpty backboneUseCRISPR; Donor cassetteExpressionMammalianAvailable SinceNov. 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSL3936 (pDonor(AAVS1)_Pse)
Plasmid#200903PurposeEncodes a mini-transposon derived from Pseudoalteromonas Tn7016 with a SA-T2A-PuroR-T2A-eGFP cargo. Total transposon size = 2,024 bp.DepositorInsertMini Tn7016 (AAVS1 NGS)
ExpressionMammalianAvailable SinceMay 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
AAVS1_dCas9-XTEN-KRAB-p2A-GFP
Plasmid#222108PurposeDonor vector for CRISPRi integration at AAVS1 with a GFP fluorophoreDepositorInsertCRISPRi-kox1(KRAB) (cas9 Human, S. pyogenes)
UseAAV and CRISPRTagsHA and P2A-GFPExpressionMammalianMutationD10A, H840AAvailable SinceOct. 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
p221-RV-dlx-dio-Tdtomato
Plasmid#185697PurposeRetroviral vector expression cre-dependent membrane tagged Tdtomato driven by the mDlx promoterDepositorInsertmembrane Tdtomato
UseCre/Lox and RetroviralTagsGap43 palmitoylationPromotermDlxAvailable SinceJuly 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
AAVS1-Puro CAG J591-scFV T-CAR
Plasmid#171961PurposeDonor plasmid for J591 T-CAR expression in human cellsDepositorInsertSynthetic J591-scFV CAR
UseCRISPR, Synthetic Biology, and TALENExpressionMammalianPromoterCAGAvailable SinceMay 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
PRS_8-Jaws-tdTomato
Plasmid#192593PurposeAAV-vector for optogenetic silencing of noradrenergic neuronsDepositorInsertJaws
UseAAVTagstdTomato-ER2ExpressionMammalianPromoterPRSX8Available SinceDec. 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
AAVS1-TRE-U2AF2-TurboID-HA-rtTA
Plasmid#226997PurposeIntegrative plasmid to express U2AF2-TurboID in mammalian cellsDepositorAvailable SinceNov. 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
AAVS1-NeoR-CAG-EGFPnls-WPRE
Plasmid#191692PurposeFor knocking pCAG-EGFPnls into AAVS1 locusDepositorInsertAAVS1 homology arms and pCAG-EGFPnls
ExpressionMammalianMutationWTAvailable SinceNov. 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
AAVS1-SA-neo-CAGGS-PE2-2A-GFP
Plasmid#180014PurposeTargeting vector for knocking in constitutively expressed PE2-2A-GFP into the human AAVS1 locusDepositorInsertPE2-2A-GFP
UseCRISPR; Human genome engineeringTagsGFPPromoterCAGAvailable SinceSept. 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAAVS1-PdTK-CAG-mCh-[uBglII]
Plasmid#107280Purposepuro-deltaTK gene-trap selection cassette (AAVS1 donor vector backbone) with constitutive mCherryDepositorInsertAAVS1 puro-deltaTK; CAG-mCherry (PPP1R12C )
Available SinceMarch 16, 2018AvailabilityAcademic Institutions and Nonprofits only -
GEARBOCS-SPARCL1-N-mCherryTag
Plasmid#218186PurposeTo tag Hevin with mCherry at the N-terminalDepositorInsertsgRNA (Sparcl1 Mouse)
UseAAV and CRISPRAvailable SinceAug. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCAX-SCLM
Plasmid#216757PurposeFor strong and long-lasting expression of SuperClomeleon in mammalian cells from the hybrid CAG promoterDepositorInsertSuperClomeleon
ExpressionMammalianMutationS30R and 11 AA deletion in the Cerulean CFP moiet…PromoterCAG (cytomegalovirus-chicken beta-actin-rabbit be…Available SinceApril 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
pEMS2155
Plasmid#141065PurposeAAV plasmid with SYN1 promoter driving expression of EmGFP. Contains WPRE.DepositorInsertSYN1-EmGFP-WPRE
UseAAVPromoterhuman SynapsinAvailable SinceApril 28, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
AAVS1-TRE3G-NODAL
Plasmid#115638PurposeFor targeted integration and inducible expression of human NODAL using doxycyclineDepositorAvailable SinceSept. 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
AAVS1-Puro CAG PSMA nanobody T-CAR
Plasmid#171962PurposeDonor plasmid for PSMA nanobody T-CAR expression in human cellsDepositorInsertSynthetic anti-PSMA nanobody CAR
UseCRISPR, Synthetic Biology, and TALENExpressionMammalianPromoterCAGAvailable SinceMay 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAVS1-Puro-TNNT2-Neo
Plasmid#214013PurposeExpression of cardiac troponin TDepositorInsertTNNT2 (TNNT2 Human)
ExpressionMammalianAvailable SinceFeb. 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
AAVS1-PuroR Xlone eGFP
Plasmid#179837PurposeDoxycycline-inducible expression of eGFPDepositorInserteGFP
UseCRISPR, Synthetic Biology, and TALEN ; Donor plas…ExpressionMammalianPromoterTRE3GSAvailable SinceJune 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
AV0.1-C346BE2.8-2
Plasmid#124969PurposeAAV control with intronDepositorInsertIntron C346
UseAAVPromoterno promoterAvailable SinceMay 7, 2019AvailabilityAcademic Institutions and Nonprofits only -
AAVS1_Cas9-hGem_gRNA2
Plasmid#217659Purposeexpresses Cas9-hGem and guideRNA targeting the human AAVS1 safe harbour locusDepositorInsertsgRNA2 targeting the human AAVS1 safe harbour locus
UseCRISPRExpressionBacterial and MammalianPromoterU6Available SinceJuly 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
hSyn-Aion-ts-mScarlet-ER
Plasmid#192577PurposeAAV-vector for optogenetic long-term silencing of neuronsDepositorInsertAion
UseAAVTagsts-mScarlet-ERExpressionMammalianMutationT59S, E83N, E90Q, E101S, V117R, E123S, C128A,D156…Promoterhuman synapsinAvailable SinceDec. 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
PRS_8-eGFP
Plasmid#192589PurposeAAV-vector for fluorescent labeling of noradrenergic neuronsDepositorInsertEGFP
UseAAVExpressionMammalianPromoterPRSX8Available SinceDec. 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
pZac2.1 GfaABC1D rM3D mCherry SV40
Plasmid#92285PurposeAAV DREADD expressionDepositorInsertrM3D
UseAAVTagsmCherryPromoterGfaABC1DAvailable SinceJune 26, 2017AvailabilityAcademic Institutions and Nonprofits only -
GEARBOCS
Plasmid#218181PurposeCRISPR tool to target astrocytesDepositorTypeEmpty backboneUseAAV and CRISPRAvailable SinceAug. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pXD042 dTomato KIKO
Plasmid#192154PurposedTomato KIKODepositorInsertdTomato
UseAAVMutationNAAvailable SinceNov. 7, 2022AvailabilityAcademic Institutions and Nonprofits only