We narrowed to 10,535 results for: Rras

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pSEVA231-CRISPR

Plasmid

#138712

Purpose

Plasmid designed to clone a specific spacer into BsaI sites to generate a CRISPR Array

Depositor

Víctor de Lorenzo

Article

Velazquez et al ACS Synth Biol. 2019 Sep 20;8(9):2186-21

Type

Empty backbone

Use

CRISPR and Synthetic Biology

Expression

Bacterial

Available Since

Sept. 25, 2020

Availability

Academic Institutions and Nonprofits only

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pLKO.1 RagB shRNA #2

Plasmid

#26628

Depositor

David Sabatini

Article

Sancak et al Science. 2008 Jun 13. 320(5882):1496-501

Insert

RagB shRNA (RRAGB Human)

Use

Lentiviral and RNAi

Expression

Mammalian

Available Since

Oct. 22, 2010

Availability

Academic Institutions and Nonprofits only
pTAG1

Plasmid

#176863

Purpose

First sgRNA in a tRNA separated array of sgRNAs

Depositor

Daniel Voytas

Article

Cody et al Nat Protoc. 2022 Oct 17. pii: 10.1038/s4

Insert

sgRNA_1

Use

CRISPR

Mutation

WT

Available Since

May 9, 2022

Availability

Academic Institutions and Nonprofits only

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pFS_0143-pET30-Fusicatenibacter_saccharivorans

Plasmid

#116952

Purpose

Expresses FsRT-Cas1-Cas2 under pT7lac promoter, encodes FsCRISPR array 1, compatible with classic acquisition readout

Depositor

Randall Platt

Article

Schmidt et al Nature. 2018 Oct;562(7727):380-385. doi:

Insert

Fusicatenibacter saccharivorans RT-Cas1-Cas2

Expression

Bacterial

Promoter

T7

Available Since

Nov. 2, 2018

Availability

Academic Institutions and Nonprofits only

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pTAG2E

Plasmid

#176865

Purpose

First sgRNA in a tRNA separated array of sgRNAs

Depositor

Daniel Voytas

Article

Cody et al Nat Protoc. 2022 Oct 17. pii: 10.1038/s4

Insert

sgRNA_2E

Use

CRISPR

Mutation

WT

Available Since

May 9, 2022

Availability

Academic Institutions and Nonprofits only

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TALEN1260

Plasmid

#32282

Depositor

Keith Joung

Article

Sander et al Nat Biotechnol. 2011 Aug 5;29(8):697-8.

Insert

TAL array targeting gria3a (gria3a Zebrafish)

Use

T7

Tags

3X Flag and WT FOKI

Expression

Mammalian

Available Since

Oct. 27, 2011

Availability

Academic Institutions and Nonprofits only

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TALEN1295

Plasmid

#32283

Depositor

Keith Joung

Article

Sander et al Nat Biotechnol. 2011 Aug 5;29(8):697-8.

Insert

TAL array targeting gria3a (gria3a Zebrafish)

Use

T7

Tags

3X Flag and WT FOKI

Expression

Mammalian

Available Since

Oct. 27, 2011

Availability

Academic Institutions and Nonprofits only

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TALEN1259

Plasmid

#32284

Depositor

Keith Joung

Article

Sander et al Nat Biotechnol. 2011 Aug 5;29(8):697-8.

Insert

TAL array targeting gria3a (gria3a Zebrafish)

Use

T7

Tags

3X Flag and WT FOKI

Expression

Mammalian

Available Since

Oct. 27, 2011

Availability

Academic Institutions and Nonprofits only

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pSEM236 - pEXP[Pmlc-2 | histamine | rpl-3 UTR]

Plasmid

#159797

Purpose

Histamine based negative selection marker to paralyze animals with arrays

Depositor

Christian Froekjaer-Jensen

Article

El Mouridi et al MicroPubl Biol. 2021 Jan 8;2021. doi: 10

Insert

pEXP[Pmlc-2 | histamine | rpl-3 UTR]

Expression

Worm

Mutation

Not applicable

Available Since

Dec. 17, 2020

Availability

Academic Institutions and Nonprofits only

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TALEN1258

Plasmid

#32281

Depositor

Keith Joung

Article

Sander et al Nat Biotechnol. 2011 Aug 5;29(8):697-8.

Insert

TAL array targeting gria3a (gria3a Zebrafish)

Use

T7

Tags

3X Flag and FOKI WT

Expression

Mammalian

Available Since

Oct. 27, 2011

Availability

Academic Institutions and Nonprofits only

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pEV_S

Plasmid

#100266

Purpose

pDSW204 carrying genes entABCDE, vibHF and a cos site, for serratiochelin and analogs production in E. coli

Depositor

Timothy Lu

Article

Cleto et al MBio. 2017 Oct 10;8(5). pii: e01474-17.

Inserts

entD (entD Escherichia coli)
entC
entE
entB
entA
vibH
vibF
cos

Use

Synthetic Biology; Expression, pbr322

Expression

Bacterial

Available Since

Nov. 3, 2017

Availability

Academic Institutions and Nonprofits only

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pVC-CRISPR1

Plasmid

#120366

Purpose

Plasmid containing shortened version of upstream V-C CRISPR array for integration assays

Depositor

Jennifer Doudna

Article

Wright et al Mol Cell. 2019 Feb 21;73(4):727-737.e3.

Insert

CRISPR1

Use

CRISPR

Available Since

April 12, 2021

Availability

Academic Institutions and Nonprofits only

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pVC-CRISPR

Plasmid

#120369

Purpose

Plasmid containing shortened versions of both natural V-C CRISPR arrays for preparing sequencing library

Depositor

Jennifer Doudna

Article

Wright et al Mol Cell. 2019 Feb 21;73(4):727-737.e3.

Inserts

CRISPR1
CRISPR2

Use

CRISPR

Available Since

April 12, 2021

Availability

Academic Institutions and Nonprofits only

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pVD_CRISPR

Plasmid

#120370

Purpose

Plasmid containing shortened version of V-D CRISPR array for integration assays

Depositor

Jennifer Doudna

Article

Wright et al Mol Cell. 2019 Feb 21;73(4):727-737.e3.

Insert

VD_CRISPR

Use

CRISPR

Available Since

April 12, 2021

Availability

Academic Institutions and Nonprofits only

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pDGE506

Plasmid

#153279

Purpose

Intermediate sgRNA array cloning vector position 4

Depositor

Johannes Stuttmann

Article

Stuttmann et al Plant J. 2021 Feb 12. doi: 10.1111/tpj.1

Type

Empty backbone

Use

CRISPR

Available Since

Aug. 6, 2020

Availability

Academic Institutions and Nonprofits only

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pDGE505

Plasmid

#153278

Purpose

Intermediate sgRNA array cloning vector position 3

Depositor

Johannes Stuttmann

Article

Stuttmann et al Plant J. 2021 Feb 12. doi: 10.1111/tpj.1

Type

Empty backbone

Use

CRISPR

Available Since

Aug. 6, 2020

Availability

Academic Institutions and Nonprofits only

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pHFX-cas3UD

Plasmid

#174385

Purpose

Plasmids for gene knockout were constructed based on suicide plasmid pHFX

Depositor

Jing Han

Article

Lin et al Commun Biol. 2021 Aug 25;4(1):1007. doi:

Insert

Cas3UD

Use

Archaeal gene knockout vector for haloferax medit…

Available Since

Nov. 11, 2021

Availability

Academic Institutions and Nonprofits only

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pRK5 Flag-RagC(L91P)

Plasmid

#99726

Purpose

Mammalian expression of Flag-RagC(L91P)

Depositor

David Sabatini, Kuang Shen

Article

Shen et al Mol Cell. 2017 Oct 17. pii: S1097-2765(1

Insert

RagC (RRAGC )

Tags

Flag

Mutation

L91P

Promoter

CMV

Available Since

Nov. 3, 2017

Availability

Academic Institutions and Nonprofits only

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pRK5 Flag-RagC(K84T)

Plasmid

#99727

Purpose

Mammalian expression of Flag-RagC(K84T)

Depositor

David Sabatini, Kuang Shen

Article

Shen et al Mol Cell. 2017 Oct 17. pii: S1097-2765(1

Insert

RagC (RRAGC )

Tags

Flag

Mutation

K84T

Promoter

CMV

Available Since

Nov. 3, 2017

Availability

Academic Institutions and Nonprofits only

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pRK5 Flag-RagA(R37P)

Plasmid

#99717

Purpose

Mammalian expression of Flag-RagA(R37P)

Depositor

David Sabatini, Kuang Shen

Article

Shen et al Mol Cell. 2017 Oct 17. pii: S1097-2765(1

Insert

RagA (RRAGA )

Tags

Flag

Mutation

R37P

Promoter

CMV

Available Since

Nov. 3, 2017

Availability

Academic Institutions and Nonprofits only

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pRK5 HA-RagC(K84T)

Plasmid

#99722

Purpose

Mammalian expression of HA-RagC(K84T)

Depositor

David Sabatini, Kuang Shen

Article

Shen et al Mol Cell. 2017 Oct 17. pii: S1097-2765(1

Insert

RagC (RRAGC )

Tags

HA

Mutation

K84T

Promoter

CMV

Available Since

Nov. 3, 2017

Availability

Academic Institutions and Nonprofits only

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pLsCas13a-gRNA-1

Plasmid

#164865

Purpose

Constitutive expression of single-spacer CRISPR array with spacer #1 targeting deGFP mRNA for LsCas13a in bacteria.

Depositor

Chase Beisel

Article

Wandera et al Methods Mol Biol. 2022;2404:135-153. doi

Insert

LsCas13a-gRNA-1

Use

CRISPR

Expression

Bacterial

Promoter

J23119

Available Since

Feb. 22, 2021

Availability

Academic Institutions and Nonprofits only

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pFUS2_a1a

Plasmid

#50680

Purpose

Intermediate array vector

Depositor

Takashi Yamamoto

Article

Sakuma et al Sci Rep. 2013 Nov 29;3:3379. doi: 10.103

Insert

LacZ + BsaI restriction sites

Use

TALEN

Available Since

May 6, 2014

Availability

Academic Institutions and Nonprofits only

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pRK5 HA-RagC(L91P)

Plasmid

#99721

Purpose

Mammalian expression of HA-RagC(L91P)

Depositor

David Sabatini, Kuang Shen

Article

Shen et al Mol Cell. 2017 Oct 17. pii: S1097-2765(1

Insert

RagC (RRAGC )

Tags

HA

Mutation

L91P

Promoter

CMV

Available Since

Nov. 3, 2017

Availability

Academic Institutions and Nonprofits only

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pRK5 HA-RagA(R37P)

Plasmid

#99713

Purpose

Mammalian expression of HA-RagA(R37P)

Depositor

David Sabatini, Kuang Shen

Article

Shen et al Mol Cell. 2017 Oct 17. pii: S1097-2765(1

Insert

RagA (RRAGA )

Tags

HA

Mutation

R37P

Promoter

CMV

Available Since

Nov. 3, 2017

Availability

Academic Institutions and Nonprofits only

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pFUS2_b4

Plasmid

#50698

Purpose

Intermediate array vector

Depositor

Takashi Yamamoto

Article

Sakuma et al Sci Rep. 2013 Nov 29;3:3379. doi: 10.103

Insert

LacZ + BsaI restriction sites

Use

TALEN

Available Since

May 6, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
ptp4a3_L (TAL 3000)

Plasmid

#35992

Depositor

Keith Joung

Article

Sander et al Nat Biotechnol. 2011 Aug 5;29(8):697-8.

Insert

TAL array targeting ptp4a3 (ptp4a3a Zebrafish)

Use

T7

Tags

3X Flag and WT FOKI

Expression

Mammalian

Available Since

March 27, 2012

Availability

Academic Institutions and Nonprofits only

Enlarge
pFUS2_b1

Plasmid

#50695

Purpose

Intermediate array vector

Depositor

Takashi Yamamoto

Article

Sakuma et al Sci Rep. 2013 Nov 29;3:3379. doi: 10.103

Insert

LacZ + BsaI restriction sites

Use

TALEN

Available Since

May 6, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
pFUS2_a2a

Plasmid

#50681

Purpose

Intermediate array vector

Depositor

Takashi Yamamoto

Article

Sakuma et al Sci Rep. 2013 Nov 29;3:3379. doi: 10.103

Insert

LacZ + BsaI restriction sites

Use

TALEN

Available Since

May 6, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
pFUS2_a2b

Plasmid

#50682

Purpose

Intermediate array vector

Depositor

Takashi Yamamoto

Article

Sakuma et al Sci Rep. 2013 Nov 29;3:3379. doi: 10.103

Insert

LacZ + BsaI restriction sites

Use

TALEN

Available Since

May 6, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
pGEX Rad

Plasmid

#11227

Depositor

Ronald Kahn

Article

Zhu et al J Biol Chem. 1995 Mar 3. 270(9):4805-12.

Insert

Rad (RRAD Human)

Tags

GST

Expression

Bacterial

Mutation

N39: deletion of amino acids 1-39

Available Since

March 1, 2006

Availability

Academic Institutions and Nonprofits only
ptp4a3_R (TAL 3001)

Plasmid

#35993

Depositor

Keith Joung

Article

Sander et al Nat Biotechnol. 2011 Aug 5;29(8):697-8.

Insert

TAL array targeting ptp4a3 (ptp4a3a Zebrafish)

Use

T7

Tags

3X Flag and WT FOKI

Expression

Mammalian

Available Since

April 27, 2012

Availability

Academic Institutions and Nonprofits only

Enlarge
MCHR1B_R (TAL 3009)

Plasmid

#36001

Depositor

Keith Joung

Article

Sander et al Nat Biotechnol. 2011 Aug 5;29(8):697-8.

Insert

TAL array targeting MCHR1B (mchr1b Zebrafish)

Use

T7

Tags

3X Flag and WT FOKI

Expression

Mammalian

Available Since

March 30, 2012

Availability

Academic Institutions and Nonprofits only

Enlarge
MCHR1B_L (TAL 3008)

Plasmid

#36000

Depositor

Keith Joung

Article

Sander et al Nat Biotechnol. 2011 Aug 5;29(8):697-8.

Insert

TAL array targeting MCHR1B (mchr1b Zebrafish)

Use

T7

Tags

3X Flag and WT FOKI

Expression

Mammalian

Available Since

March 30, 2012

Availability

Academic Institutions and Nonprofits only

Enlarge
pBABE Rad C249

Plasmid

#11215

Depositor

Ronald Kahn

Article

Moyers et al J Biol Chem. 1997 May 2. 272(18):11832-9

Insert

Rad (RRAD Human)

Use

Retroviral

Expression

Mammalian

Mutation

C249: deletion of amino acids 248-308

Available Since

March 1, 2006

Availability

Academic Institutions and Nonprofits only
GDF2_R (TAL 3011)

Plasmid

#36003

Depositor

Keith Joung

Article

Sander et al Nat Biotechnol. 2011 Aug 5;29(8):697-8.

Insert

TAL array targeting GDF2 (gdf2 Zebrafish)

Use

T7

Tags

3X Flag and WT FOKI

Expression

Mammalian

Available Since

April 27, 2012

Availability

Academic Institutions and Nonprofits only

Enlarge
GDF2_L (TAL 3010)

Plasmid

#36002

Depositor

Keith Joung

Article

Sander et al Nat Biotechnol. 2011 Aug 5;29(8):697-8.

Insert

TAL array targeting GDF2 (gdf2 Zebrafish)

Use

T7

Tags

3X Flag and WT FOKI

Expression

Mammalian

Available Since

April 27, 2012

Availability

Academic Institutions and Nonprofits only

Enlarge
pDS_TempRecording

Plasmid

#241789

Purpose

pBBR1MCS replicative vector for Cas1-Cas2-RT and Spacer for transcriptional recording with a temperature sensor

Depositor

Marc Güell

Article

Nevot et al ACS Synth Biol. 2025 Jun 22. doi: 10.102

Insert

FBFP, Cas1-Cas2-RT_and CRISPR Array

Use

Synthetic Biology

Expression

Bacterial

Mutation

WT

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pGL4.26_4xUAS_DSCP_GFP

Plasmid

#111931

Purpose

Vector to express GFP (under the control of the DSCP and an array of 4 UAS sites)

Depositor

Alexander Stark

Article

Arnold et al EMBO J. 2018 Aug 15;37(16). pii: embj.20

Insert

4xUAS GFP

Expression

Insect

Available Since

April 19, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pTAG4E

Plasmid

#176868

Purpose

First sgRNA in a tRNA separated array of sgRNAs

Depositor

Daniel Voytas

Article

Cody et al Nat Protoc. 2022 Oct 17. pii: 10.1038/s4

Insert

sgRNA_4E

Use

CRISPR

Mutation

WT

Available Since

May 9, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pTAG5

Plasmid

#176869

Purpose

First sgRNA in a tRNA separated array of sgRNAs

Depositor

Daniel Voytas

Article

Cody et al Nat Protoc. 2022 Oct 17. pii: 10.1038/s4

Insert

sgRNA_5

Use

CRISPR

Mutation

WT

Available Since

May 9, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pTAG6E

Plasmid

#176870

Purpose

First sgRNA in a tRNA separated array of sgRNAs

Depositor

Daniel Voytas

Article

Cody et al Nat Protoc. 2022 Oct 17. pii: 10.1038/s4

Insert

sgRNA_6E

Use

CRISPR

Mutation

WT

Available Since

May 9, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pTAG2

Plasmid

#176864

Purpose

First sgRNA in a tRNA separated array of sgRNAs

Depositor

Daniel Voytas

Article

Cody et al Nat Protoc. 2022 Oct 17. pii: 10.1038/s4

Insert

sgRNA_2

Use

CRISPR

Mutation

WT

Available Since

May 9, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pTAG3

Plasmid

#176866

Purpose

First sgRNA in a tRNA separated array of sgRNAs

Depositor

Daniel Voytas

Article

Cody et al Nat Protoc. 2022 Oct 17. pii: 10.1038/s4

Insert

sgRNA_3

Use

CRISPR

Mutation

WT

Available Since

May 9, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pTAG4

Plasmid

#176867

Purpose

First sgRNA in a tRNA separated array of sgRNAs

Depositor

Daniel Voytas

Article

Cody et al Nat Protoc. 2022 Oct 17. pii: 10.1038/s4

Insert

sgRNA_4

Use

CRISPR

Mutation

WT

Available Since

May 9, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pJL50

Plasmid

#174068

Purpose

Expresses 97-3 zinc finger array fused to VP16 in S. cerevisiae. Parent plasmid for chromatin regulator library.

Depositor

Albert Keung

Article

Lee et al Cell Syst. 2021 Aug 24. pii: S2405-4712(

Insert

pTEF1-3NLS-97-3ZF-VP16

Tags

FLAG

Expression

Yeast

Promoter

pTEF1

Available Since

Dec. 17, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pBzCas13b-gRNA-2

Plasmid

#164859

Purpose

Constitutive expression of single-spacer CRISPR array with spacer #2 targeting deGFP mRNA for BzCas13b in bacteria.

Depositor

Chase Beisel

Article

Wandera et al Methods Mol Biol. 2022;2404:135-153. doi

Insert

BzCas13b-gRNA-2

Expression

Bacterial

Promoter

J23119

Available Since

Feb. 22, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pBzCas13b-gRNA-3

Plasmid

#164860

Purpose

Constitutive expression of single-spacer CRISPR array with spacer #3 targeting deGFP mRNA for BzCas13b in bacteria.

Depositor

Chase Beisel

Article

Wandera et al Methods Mol Biol. 2022;2404:135-153. doi

Insert

BzCas13b-gRNA-3

Expression

Bacterial

Promoter

J23119

Available Since

Feb. 22, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pLsCas13a-gRNA-3

Plasmid

#164867

Purpose

Constitutive expression of single-spacer CRISPR array with spacer #3 targeting deGFP mRNA for LsCas13a in bacteria.

Depositor

Chase Beisel

Article

Wandera et al Methods Mol Biol. 2022;2404:135-153. doi

Insert

LsCas13a-gRNA-3

Use

CRISPR

Expression

Bacterial

Promoter

J23119

Available Since

Feb. 22, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pLsCas13a-gRNA-2

Plasmid

#164866

Purpose

Constitutive expression of single-spacer CRISPR array with spacer #2 targeting deGFP mRNA for LsCas13a in bacteria.

Depositor

Chase Beisel

Article

Wandera et al Methods Mol Biol. 2022;2404:135-153. doi

Insert

LsCas13a-gRNA-2

Use

CRISPR

Expression

Bacterial

Promoter

J23119

Available Since

Feb. 22, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge

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