169,450 results
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Plasmid#84563PurposeBicistronic vector for expression of O. sativa E3 ubiquitin ligase and single chain variable fragment of GCN4 antibody fused to superfolder GFPDepositorInsertTransport inhibitor response 1 (E3 ligase domain), IRES, single chain variable fragment-superfolder GFP
UseLentiviralTagsmyc tag (9x)Available SinceDec. 20, 2016AvailabilityAcademic Institutions and Nonprofits only -
pDIRECT_22C
Plasmid#91135PurposeDirect Cloning, Type: T-DNA, Engineering Reagent: 35S:Csy4-P2A-AtCas9 + CmYLCV:gRNAs with Csy4 spacers, Plant Selection: 2x35S:npt IIDepositorInsertEngineering Reagent: 35S:Csy4-P2A-AtCas9 + CmYLCV:gRNAs with Csy4 spacers
UseCRISPRExpressionPlantAvailable SinceJuly 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
pYES-mtGFP
Plasmid#45053DepositorInsertmtGFP
ExpressionYeastPromoterGALAvailable SinceMay 24, 2013AvailabilityAcademic Institutions and Nonprofits only -
GFP-ERK1
Plasmid#14747DepositorAvailable SinceJune 12, 2007AvailabilityAcademic Institutions and Nonprofits only -
pHR_MFE1_hrGFP
Plasmid#84617PurposeHR donor to integrate UAS1B8TEF(136)-hrGFP-CYC1t into MFE1 locusDepositorInserthrGFP
UseSynthetic BiologyExpressionYeastPromoterUAS1B8-TEF(136)Available SinceDec. 22, 2016AvailabilityAcademic Institutions and Nonprofits only -
pSEED-sfGFP
Plasmid#219639PurposePermits GoldenGate assembly (BsmBI) of donor plasmids to knock-in sfGFP using the SEED/Harvest methodDepositorInsertsfGFP SEED Cassette
Available SinceNov. 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
pTECH-MmPylRS(IPYE)
Plasmid#104073PurposeExpression plasmid encoding MmPylRS(IPYE) and pylTDepositorInsertPlpp MmPylRS(IPYE); PproK pyl
ExpressionBacterialMutationContains activating mutations V31I, T56P, H62Y, A…PromoterPlpp and PproKAvailable SinceJan. 10, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMax-E2F1
Plasmid#16007DepositorAvailable SinceOct. 31, 2007AvailabilityAcademic Institutions and Nonprofits only -
pWUR790
Plasmid#101723PurposeSynthetic codon optimized P. furiosus ago with N-terminal Strep(II)-tag in pCDF-1b vector, expression vector for PfAgoDepositorInsertPfAgo
TagsStrep(II)-tagExpressionBacterialPromoterT7 PromotorAvailable SinceNov. 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
pEGFPC1-human APPL1
Plasmid#22198DepositorInsertAdaptor protein containing PH domain, PTB domain and Leucine zipper motif (APPL1 Human)
TagsGFPExpressionBacterial and MammalianAvailable SinceOct. 21, 2009AvailabilityAcademic Institutions and Nonprofits only -
pGS-21a his6-GST-WTPhi29DNAP
Plasmid#166863PurposeTo produce a dual affinity-tagged Phi29 DNA polymeraseDepositorInsertPhi29 Polymerase codon-optimized for overexpression in E. coli
TagsGST and his6ExpressionBacterialAvailable SinceMarch 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
pSLQ1658-dCas9-EGFP
Plasmid#51023PurposeTemplate for NLS-dCas9-NLS-EGFP fusion protein for CRISPR imaging (the recipient vector can be TetON 3G promoter system)DepositorInsertdCas9 fuse to EGFP
UseCRISPR and RetroviralTagsEGFPExpressionMammalianPromoterMSCV LTR promoterAvailable SinceFeb. 11, 2014AvailabilityAcademic Institutions and Nonprofits only -
V5-ERG
Plasmid#118623PurposeExpresses V5 tagged ERG proteinDepositorAvailable SinceDec. 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-LacR-TopBP1
Plasmid#31317DepositorAvailable SinceNov. 9, 2011AvailabilityAcademic Institutions and Nonprofits only -
pChillyBoys_gen1
Plasmid#228495PurposepACYCDuet-1 encoding Oleispira antarctica GroEL and GorES low temp chaperonins for enhanced low-temperature induction (E. coli based protein expression)DepositorInsertsgroEL
groES
ExpressionBacterialPromoterT7 PromoterAvailable SinceJan. 3, 2025AvailabilityAcademic Institutions and Nonprofits only -
pSLQ9834_sgBB: pHR-hU6-CasMINI sgRNA_#2; EF1a-Puro-T2A-BFP- WPRE
Plasmid#180280PurposeThe CasMINI sgRNA cloning backbone with sites 'BsmBI' for inserting new guide sequences.DepositorInsertCasMINI sgRNA (backbone) and BFP
UseLentiviralExpressionMammalianPromoterU6Available SinceMarch 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
pUCmini-iCAP-AAV9.452sub.LUNG1
Plasmid#184592Purposenon-standard AAV2 rep-AAV9.452sub.LUNG1 cap plasmid with AAV cap expression controlled by a tTA-TRE amplification system for mouse lung transduction after intravenous injectionDepositorInsertSynthetic construct isolate AAV9.452sub.LUNG1 VP1 gene
UseAAVExpressionMammalianMutation7 amino acid substitution between VP1 452 and VP1…Promoterp41Available SinceJuly 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCuo CA Rac1
Plasmid#84643Purposeconstitutively active Rac1 under cumate-inducible promoter in Lentiviral vector. Also expresses RFP and puromycin resistance.DepositorInsertRac1 (RAC1 Human)
UseLentiviralExpressionMammalianMutationConstitutively Active, Q61LPromotercumate-inducible promoterAvailable SinceApril 3, 2017AvailabilityAcademic Institutions and Nonprofits only -
pTW199b2
Plasmid#136928PurposeDirected evolution of SpCas9DepositorInsertgIII
UseCRISPRExpressionBacterialMutationnoneAvailable SinceFeb. 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGL4.23-GW
Plasmid#60323PurposeThis is a modified version of the pGL4.23 vector from Promega, containing a Gateway cassette upstream of the minimal promoter. This vector can be used for for Gateway cloning of candidate enhancer elements. As negative control, use pGL4.23 without the gateway cassette (available from Promega).DepositorTypeEmpty backboneUseLuciferaseTagsLuciferaseExpressionMammalianAvailable SinceDec. 2, 2014AvailabilityAcademic Institutions and Nonprofits only -
pOTTC407 - pAAV EF1a eGFP
Plasmid#60058PurposeAn AAV packaging vector that expresses enhanced green fluorescent protein under control of the EF1a promoter.DepositorInsertenhanced Green Fluorescent Protein
UseAAVExpressionMammalianPromoterEF1aAvailable SinceOct. 9, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCRISPRyl_D17
Plasmid#84611PurposeIntroduces DSB at D17 locus in Y. lipolyticaDepositorInsertCas9
UseCRISPR and Synthetic BiologyExpressionYeastPromoterUAS1B8-TEF(136)Available SinceDec. 22, 2016AvailabilityAcademic Institutions and Nonprofits only -
pAAV-EFS-DIO-GRAB_Ado1.0
Plasmid#153291PurposeExpress the adenosine sensor GRAB_Ado1.0 in Cre positive cellsDepositorInsertAdenosine sensor GRAB_Ado1.0
UseAAVPromoterEFSAvailable SinceJuly 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
AiP13781 - pAAV-AiE0452h-minBG-iCre(R297T)-BGHpA (Alias: CN3781)
Plasmid#199777Purposeenhancer AAV for Cre recombinase expression in striatal indirect pathway D2-MSNsDepositorHas ServiceAAV PHP.eBInsertiCre(R297T)
UseAAVMutationR297T for reduced recombinase efficiency.Available SinceJuly 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
His6-MBP-uTEV3
Plasmid#135464PurposeBacterial expression of uTEV3 (full-length) proteaseDepositorInsertHis6-MBP-uTEV3
TagsMBP, His6ExpressionBacterialMutationS219V mutation (improves stability) , I138T/S153N…PromoterT5Available SinceJan. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
AttB_ACE2-2A-TMPRSS2_IRES_iRFP670-H2A-P2A-PuroR
Plasmid#190077Purposeexpress human ACE2 and human TMPRSS2 with selectable markers iRFP670 and puromycin resistance geneDepositorAvailable SinceSept. 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
p5E-slc1a3b
Plasmid#170205PurposeGateway 5’ entry vector with the slc1a3b promoterDepositorAvailable SinceJune 1, 2021AvailabilityAcademic Institutions and Nonprofits only -
UbiGate Components (set1)
Plasmid Kit#1000000144PurposeGenerate bacterial expression cassettes of up to six ORFs simultaneously; also contains ubiquitin, the E1 UBA1, an E2 collection, and a selection of E3s and their substrates for ubiquitination assaysDepositorAvailable SinceFeb. 1, 2019AvailabilityAcademic Institutions and Nonprofits only -
1099 pcDNA GFP FKHR AAA
Plasmid#9023DepositorAvailable SinceDec. 7, 2005AvailabilityAcademic Institutions and Nonprofits only -
pKOS6b-KOpstS
Plasmid#237503PurposeKnockout of pstSDepositorInsertphosphate ABC transporter substrate-binding protein PstS
ExpressionBacterialAvailable SinceSept. 16, 2025AvailabilityAcademic Institutions and Nonprofits only