176,103 results

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pDONOR-tagBFP-PSM-dTOMATO

Plasmid

#100604

Purpose

Donor scafold with dTOMATO positive selection module, and tagBFP negative selection module

Depositor

Jens Schwamborn

Article

Arias-Fuenzalida et al Stem Cell Reports. 2017 Nov 14;9(5):1423

Type

Empty backbone

Use

Synthetic Biology

Tags

dTOMATO-T2A-Puro and tagBFP

Available Since

May 30, 2018

Availability

Academic Institutions and Nonprofits only

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EMTB-mNeonGreen

Plasmid

#137802

Purpose

Visualization of microtubules

Depositor

Dorus Gadella

Article

Chertkova et al bioRxiv (2020) 160374; doi: https://doi.

Insert

Ensconsin microtubule binding domain

Expression

Mammalian

Promoter

CMV

Available Since

March 30, 2020

Availability

Academic Institutions and Nonprofits only

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LentiCRISPR v2-sgLKB1 clone3

Plasmid

#162127

Purpose

Lentiviral sgRNA plasmid targeting human LKB1

Depositor

Boyi Gan

Article

Lee et al Nat Cell Biol. 2020 Feb;22(2):225-234. d

Insert

sgLKB1 (STK11 Human)

Use

Lentiviral

Available Since

Jan. 26, 2021

Availability

Academic Institutions and Nonprofits only

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pK18sB

Plasmid

#177838

Purpose

Plasmid for gene replacement using kanamycin/sucrose selection/counterselection. Derived from pK18mobsacB but reduced in size and lacking oriT required for conjugation.

Depositor

Gregg Beckham, Christopher Johnson

Article

Jayakody et al Energy Environ. Sci., 2018,11, 1625-1638

Type

Empty backbone

Use

Bacterial gene replacement

Available Since

Dec. 3, 2021

Availability

Academic Institutions and Nonprofits only

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pDONR221_SLC25A44

Plasmid

#132042

Purpose

Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

Depositor

RESOLUTE Consortium, Giulio Superti-Furga

Article

Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

Insert

SLC25A44 (SLC25A44 Human)

Expression

Mammalian

Available Since

Nov. 4, 2019

Availability

Industry, Academic Institutions, and Nonprofits

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pAJM.773

Plasmid

#108527

Purpose

VanRAM + PVanCC-YFP Reporter. Van sensor for use in non-Marionette strain.

Depositor

Christopher Voigt

Article

Meyer et al Nat Chem Biol. 2018 Nov 26. pii: 10.1038

Insert

VanRAM + PVanCC-YFP

Expression

Bacterial

Available Since

July 6, 2018

Availability

Academic Institutions and Nonprofits only

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pD649-HAsp-NOTCH4(Trunc1)-Fc(DAPA)-AviTag-6xHis

Plasmid

#157040

Purpose

Mammalian expression of cell-surface protein partial extracellular domain fused to Fc(DAPA)-Avi-6xHis. Protein is secreted from cells.

Depositor

Chris Garcia

Article

Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.

Insert

NOTCH4 (NOTCH4 Human)

Tags

Fc(DAPA)-AviTag-6xHis

Expression

Mammalian

Mutation

Fc region of human IgG contains D265A and P329A m…

Promoter

CMV/SP6

Available Since

March 8, 2023

Availability

Academic Institutions and Nonprofits only

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Flag-SIRT1

Plasmid

#1791

Purpose

Mammalian expression of flag-tagged SIRT1

Depositor

Michael Greenberg

Article

Brunet et al Science 2004 Mar 26;303(5666):2011-5. Ep

Insert

SIRT1 (SIRT1 Human)

Tags

Flag

Expression

Mammalian

Available Since

Dec. 7, 2005

Availability

Academic Institutions and Nonprofits only

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pGL4 [luc2P/IL-2/Hygro] Vector

Plasmid

#236930

Purpose

Express IL-2 in luciferase reporter assay

Depositor

Promega Corporation

Has Service

DNA

Insert

IL-2 (IL2 Human)

Use

Luciferase

Mutation

None

Available Since

July 14, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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pFastBac-rAPOBEC1-pAG-hADAR2dd

Plasmid

#248100

Purpose

Expresses rAPOBEC1-pAG-hADAR2dd in insect cells

Depositor

Yangming Wang

Article

Cheng et al Nat Methods. 2025 Sep;22(9):1824-1835. d

Inserts

rAPOBEC1 (Apobec1 Rat)
protein A/G
hADAR2dd (ADARB1 Human)

Tags

His and Strep

Expression

Insect

Available Since

Dec. 2, 2025

Availability

Academic Institutions and Nonprofits only

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pInducer-YAP1

Plasmid

#213583

Purpose

Doxycycline inducible expression of YAP1 wild type cDNA in mammalian cells

Depositor

Channing Der

Article

Edwards et al Cancer Res. 2023 Nov 7. doi: 10.1158/000

Insert

YAP1 (YAP1 Human)

Use

Lentiviral

Tags

HA

Expression

Mammalian

Available Since

March 12, 2024

Availability

Academic Institutions and Nonprofits only

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MAC_N_SOX10

Plasmid

#167791

Purpose

Expresses SOX10 in mammalian cells

Depositor

Markku Varjosalo

Article

Goos et al Nat Commun. 2022 Feb 9;13(1):766. doi: 1

Insert

SOX10 (SOX10 Human)

Tags

MAC

Expression

Mammalian

Mutation

no mutations

Available Since

Jan. 24, 2022

Availability

Academic Institutions and Nonprofits only

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hACE2

Plasmid

#1786

Purpose

Mammalian expression of human ACE2

Depositor

Hyeryun Choe

Article

Li et al Nature. 2003 Nov 27. 426(6965):450-4.

Has Service

Cloning Grade DNA

Insert

Angiotensin-converting enzyme 2 (ACE2 Human)

Tags

C9 (GTETSQVAPA)

Expression

Mammalian

Available Since

Feb. 14, 2006

Availability

Industry, Academic Institutions, and Nonprofits

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AAV-hSyn-RiboL1-jGCaMP8m

Plasmid

#169248

Purpose

AAV transfer plasmid for neuronal expression of soma-targeted (RL-10, ribosomal tag) jGCaMP8m, with a flexible GS-linker sequence attaching the ribosomal tag to the jGCaMP8m protein.

Depositor

Marianne Fyhn

Article

Grodem et al Nat Commun. 2023 Feb 4;14(1):608. doi: 1

Insert

RiboL1-jGCaMP8m

Use

AAV

Tags

6xHis

Expression

Mammalian

Promoter

hSyn

Available Since

Dec. 10, 2021

Availability

Academic Institutions and Nonprofits only

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YFP-PCAF-C2

Plasmid

#134572

Purpose

Expresses YFP-PCAF fusion protein

Depositor

Susan Janicki

Article

Rafalska-Metcalf et al PLoS One. 2010 Apr 21;5(4):e10272. doi:

Insert

PCAF (Kat2b Mouse)

Tags

YFP

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 10, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA3-EGFP-C4-Nrf2

Plasmid

#21549

Depositor

Yue Xiong

Article

Furukawa et al Mol Cell Biol. 2005 Jan . 25(1):162-71.

Insert

nuclear factor (erythroid-derived 2)-like 2 (NFE2L2 Human)

Tags

EGFP and Myc

Expression

Mammalian

Available Since

July 1, 2009

Availability

Academic Institutions and Nonprofits only

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pGM190oriT

Plasmid

#115679

Purpose

E. coli-Streptomyces shuttle vector for gene expression

Depositor

Günther Muth

Insert

oriT, aphII, tsr

Expression

Bacterial

Promoter

tipA

Available Since

Feb. 27, 2019

Availability

Academic Institutions and Nonprofits only

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Sirius/pcDNA3

Plasmid

#51957

Purpose

mammalian expression of Sirius, a pH-insensitive and photostable ultramarine fluorescent protein

Depositor

Takeharu Nagai

Article

Tomosugi et al Nat Methods. 2009 May;6(5):351-3. doi: 1

Insert

Sirius

Expression

Mammalian

Available Since

April 15, 2014

Availability

Academic Institutions and Nonprofits only

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iRFP-PAmCherry1-PH_GRP1

Plasmid

#92168

Purpose

PI(3,4,5)P3 single molecule fluorescent reporter

Depositor

Xuelin Lou

Article

Ji et al J Biol Chem. 2015 Nov 6;290(45):26978-93

Insert

iRFP-PAmCherry1-PHgrp1

Tags

iRFP-PAmCherry1

Expression

Mammalian

Promoter

CMV

Available Since

June 21, 2017

Availability

Academic Institutions and Nonprofits only

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pCAG1.1-TSSK2/3xFLAG

Plasmid

#199625

Purpose

Expression vector of mouse TSSK2. 3xFLAG tag at C-terminus.

Depositor

Masahito Ikawa

Article

Shimada et al Proc Natl Acad Sci U S A. 2023 Mar 14;12

Insert

Tssk2 mRNA (Tssk2 Mouse)

Tags

3xFLAG

Promoter

CAG

Available Since

April 24, 2023

Availability

Academic Institutions and Nonprofits only

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mAzurite-16xRhoBAST

Plasmid

#196347

Purpose

Expression of mAzurite mRNA with 16xRhoBAST in 3'UTR

Depositor

Murat Sunbul

Article

Englert et al Nat Commun. 2023 Jun 30;14(1):3879. doi:

Insert

mAzurite-16xRhoBAST

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 15, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-GFAP-eGFP-shLacZ

Plasmid

#223222

Purpose

mir30 based shRNA strategy, control shRNA

Depositor

Li-Huei Tsai

Article

Murdock et al Nature. 2024 Mar;627(8002):149-156. doi:

Insert

shLacZ

Use

AAV

Available Since

July 29, 2024

Availability

Academic Institutions and Nonprofits only

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pScarlet-Borcs7

Plasmid

#118748

Purpose

Expresses scarlet tagged Borcs7

Depositor

Beverly Koller

Insert

Borcs7 cDNA (2010012O05Rik Mouse)

Tags

mScarlet

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 27, 2018

Availability

Academic Institutions and Nonprofits only

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pETM33_CALM1_EF-hand

Plasmid

#178468

Purpose

Bacterial expression of human domain with His-tag and GST tag

Depositor

Ylva Ivarsson

Article

Benz et al Mol Syst Biol. 2022 Jan;18(1):e10584. do

Insert

CALM1_EF-hand (CALM1 Human, Synthetic)

Tags

His-GST

Expression

Bacterial

Promoter

T7/LacO

Available Since

March 22, 2022

Availability

Academic Institutions and Nonprofits only

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pTRIPZ

Plasmid

#206981

Purpose

empty pTRIPZ vector for cloning of doxycycline-inducible gene expression

Depositor

Ming Chen

Article

Loh et al Metabolites. 2023 Aug 11;13(8):939. doi:

Type

Empty backbone

Use

Lentiviral

Expression

Mammalian

Available Since

Sept. 20, 2023

Availability

Academic Institutions and Nonprofits only

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Venus-2xFYVE

Plasmid

#234746

Purpose

Mammalian expression of endosome marker Venus-2xFYVE

Depositor

Nevin Lambert

Article

Jang et al Elife. 2024 Nov 8;13:RP97033. doi: 10.75

Insert

Venus-2xFYVE

Expression

Mammalian

Promoter

CMV

Available Since

April 3, 2025

Availability

Academic Institutions and Nonprofits only

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mCh-Rab7A

Plasmid

#61804

Purpose

Human Rab7A fluorescently tagged with mCh on the N-terminus for mammalian expression

Depositor

Gia Voeltz

Article

Rowland et al Cell. 2014 Nov 20;159(5):1027-41. doi: 1

Insert

Rab7A (RAB7A Human)

Tags

mCh

Expression

Mammalian

Mutation

None

Promoter

CMV

Available Since

Feb. 2, 2015

Availability

Academic Institutions and Nonprofits only

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pL-CRISPR.EFS.GFP

Plasmid

#57818

Purpose

Lentiviral CRISPR-Cas9 delivery for SpCas9 and sgRNA. Coexpresses eGFP via P2A cleavage site. EFS Promoter driven

Depositor

Benjamin Ebert

Article

Heckl et al Nat Biotechnol. 2014 Jun 22. doi: 10.103

Inserts

SpCas9
Sp sgRNA scaffold
EFS (EEF1A1 Human)
P2A-eGFP

Use

CRISPR and Lentiviral

Tags

FLAG

Promoter

EFS and hU6

Available Since

Aug. 20, 2014

Availability

Academic Institutions and Nonprofits only

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pLKO.1 EF1a-EGFP

Plasmid

#231925

Purpose

3rd generation lentiviral transfer plasmid with EGFP reporter gene

Depositor

Sriram Neelamegham

Article

Yang et al Nucleic Acids Res. 2025 Feb 27;53(5):gka

Insert

EGFP

Use

Lentiviral

Promoter

EF-1a

Available Since

March 4, 2025

Availability

Academic Institutions and Nonprofits only

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pDONR223-COL4A3BP

Plasmid

#23569

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

COL4A3BP (CERT1 Human)

Use

Gateway Cloning

Available Since

July 30, 2010

Availability

Academic Institutions and Nonprofits only