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We narrowed to 146 results for: Tn5

Showing: 51 - 100 of 146 results

  8. pLP-pTac-Tn5-chlorR

    Plasmid
    #199994
    Purpose
    Landing Pad with T7 RNAP circuit variant T15. No seed promoter. pTac-Tn5 transposase. In dap auxotrophic conjugation donor. This material contains 2 plasmids. Look below for more information.
    Depositor
    Farren Isaacs
    Article
    Patel et al Cell. 2022 Apr 28;185(9):1487-1505.e14.
    Insert
    Landing Pads
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    March 14, 2024
    Availability
    Academic Institutions and Nonprofits only
    199994-plasmid-map-sequence-id-423257

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  11. VR124 pETv2 10His-pG-Tn5 (E54K, L372P)

    Plasmid
    #198468
    Purpose
    E. coli protein expression plasmid encoding hyperactive Tn5 transposase fused to protein G, in T7 Express lysY/Iq protein expression strain.
    Depositor
    Viviana Risca
    Article
    Soroczynski et al bioRxiv 2024.07.11.602973
    Insert
    10His-pG-Tn5 (E54K, L372P)
    Use
    Tags
    10His-pG
    Expression
    Bacterial
    Mutation
    E54K, L372P
    Promoter
    Consensus φ10 T7 Promoter
    Available since
    April 14, 2023
    Availability
    Academic Institutions and Nonprofits only
    198468-plasmid-map-sequence-id-390256

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  14. pD649-HAsp-CNTN5-Fc(DAPA)-AviTag-6xHis

    Plasmid
    #157026
    Purpose
    Mammalian expression of cell-surface protein extracellular domain fused to Fc(DAPA)-Avi-6xHis. Protein is secreted from cells.
    Depositor
    Chris Garcia
    Article
    Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.
    Insert
    CNTN5 (CNTN5 Human)
    Use
    Tags
    Fc(DAPA)-AviTag-6xHis
    Expression
    Mammalian
    Mutation
    Fc region of human IgG contains D265A and P329A m…
    Promoter
    CMV/SP6
    Available since
    March 8, 2023
    Availability
    Academic Institutions and Nonprofits only
    157026-plasmid-map-sequence-id-303161

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  15. pEZ-Frt-lox-DT

    Plasmid
    #11736
    Depositor
    Klaus Rajewsky
    Insert
    pEZ-Frt-lox-DT
    Use
    Cre/Lox; /frt
    Tags
    Expression
    Mutation
    Promoter
    Available since
    May 17, 2006
    Availability
    Academic Institutions and Nonprofits only
    11736-plasmid-map-sequence-id-7056

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  16. pGLU_103

    Plasmid
    #225785
    Purpose
    This is the promoter region taken from upstream of Tn5 transposase in pBAM1. It can be considered the "default" promoter to use.
    Depositor
    Nili Ostrov
    Insert
    Ptn5_[Tn5]
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    N/A
    Promoter
    Available since
    Sept. 27, 2024
    Availability
    Academic Institutions and Nonprofits only
    225785-plasmid-map-sequence-id-446997

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  17. pRL1058

    Plasmid
    #70686
    Purpose
    Tn5-based plasmid for transposition in Anabaena sp. strain PCC 7120. Bears an oriV, so when excised from a chromosome or megaplasmid it can carry its neighboring sequences when returned to E. coli.
    Depositor
    Peter Wolk
    Article
    Wolk et al Proc Natl Acad Sci U S A. 1991 Jun 15;88
    Insert
    Transposon Tn5-1058
    Use
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Dec. 4, 2015
    Availability
    Academic Institutions and Nonprofits only
    70686-plasmid-map-sequence-id-126440

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  18. pEZ-Frt-lox-DT

    Plasmid
    #11736
    Depositor
    Klaus Rajewsky
    Insert
    pEZ-Frt-lox-DT
    Use
    Cre/Lox; /frt
    Tags
    Expression
    Mutation
    Promoter
    Available since
    May 17, 2006
    Availability
    Academic Institutions and Nonprofits only
    11736-plasmid-map-sequence-id-7056

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  20. pMis1_1B

    Plasmid
    #192079
    Purpose
    A pBBR-1 based vector with IncP group plasmid compatibility for expression in Methylorubrum extorquens using rhamnose dependent induction
    Depositor
    Markus Buchhaupt
    Article
    Poschel et al Microbiologyopen. 2022 Oct;11(5):e1325.
    Inserts
    KanR
    rhaR
    rhaS
    pBBR1 rep
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Dec. 15, 2022
    Availability
    Academic Institutions and Nonprofits only
    192079-plasmid-map-sequence-id-380008

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  29. RP4-7

    Plasmid
    #79811
    Purpose
    Modified broad host range plasmid RP4. Chloramphenicol resistant. Helper plasmid
    Depositor
    Michael Hynes
    Article
    Quandt et al Plasmid. 2004 Jul;52(1):1-12.
    Insert
    Chloramphenicol acetyltransferase
    Use
    Helper plasmid for mobilization
    Tags
    Expression
    Mutation
    Promoter
    Own promoter
    Available since
    Aug. 19, 2016
    Availability
    Academic Institutions and Nonprofits only

  31. RP4-6

    Plasmid
    #80385
    Purpose
    Helper plasmid for mobilisation derived from RP4. Spectinomycin/Streptomycin resistant
    Depositor
    Michael Hynes
    Article
    Quandt et al Plasmid. 2004 Jul;52(1):1-12.
    Insert
    Spectinomycin/Streptomycin
    Use
    Helper bhr plasmid for mobilizing other plasmids …
    Tags
    none
    Expression
    Mutation
    Promoter
    None
    Available since
    Aug. 19, 2016
    Availability
    Academic Institutions and Nonprofits only

  32. RP4-8

    Plasmid
    #79813
    Purpose
    Modified broad host range plasmid RP4. Gentamicin resistant. Helper plasmid for conjugation
    Depositor
    Michael Hynes
    Article
    Quandt et al Plasmid. 2004 Jul;52(1):1-12.
    Insert
    Gentamicin resistance gene
    Use
    Helper bhr plasmid for mobilizing other plasmids …
    Tags
    Expression
    Mutation
    Promoter
    Own promoter
    Available since
    Aug. 19, 2016
    Availability
    Academic Institutions and Nonprofits only

  34. RP4-11

    Plasmid
    #79812
    Purpose
    Modified RP4 plasmid with Km resistance and sacB gene. Easily curable helper plasmid
    Depositor
    Michael Hynes
    Article
    Quandt et al Plasmid. 2004 Jul;52(1):1-12.
    Insert
    Kanamycin cassette and SacB gene (sacB )
    Use
    Helper plasmid for mobilization
    Tags
    Expression
    Mutation
    Promoter
    Km
    Available since
    Aug. 19, 2016
    Availability
    Academic Institutions and Nonprofits only

  35. pRT802

    Plasmid
    #68113
    Purpose
    Integration vector derived from Streptomyces phage phiBT1. Kanamycin resistance gene, oriT, ColE1 origin of replication.
    Depositor
    Maggie Smith
    Article
    Gregory et al J Bacteriol. 2003 Sep;185(17):5320-3.
    Inserts
    aac3(IV)
    aph11
    oriT
    attP
    int
    Use
    Streptomyces integrating vector
    Tags
    Expression
    Mutation
    Promoter
    Available since
    July 9, 2019
    Availability
    Academic Institutions and Nonprofits only
    68113-plasmid-map-sequence-id-249001

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  36. pGLU_131

    Plasmid
    #225824
    Purpose
    Tn libraries Tn5 variant TET backbone
    Depositor
    Nili Ostrov
    Type
    Empty backbone
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    N/A
    Promoter
    Available since
    Nov. 7, 2024
    Availability
    Academic Institutions and Nonprofits only
    225824-plasmid-map-sequence-id-451452

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  37. pGLU_133

    Plasmid
    #225826
    Purpose
    Tn libraries Tn5 variant SPEC backbone
    Depositor
    Nili Ostrov
    Type
    Empty backbone
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    N/A
    Promoter
    Available since
    Oct. 21, 2024
    Availability
    Academic Institutions and Nonprofits only
    225826-plasmid-map-sequence-id-449894

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  38. pGLU_132

    Plasmid
    #225825
    Purpose
    Tn libraries Tn5 variant CAM backbone
    Depositor
    Nili Ostrov
    Type
    Empty backbone
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    N/A
    Promoter
    Available since
    Oct. 21, 2024
    Availability
    Academic Institutions and Nonprofits only
    225825-plasmid-map-sequence-id-449892

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  39. pGLU_129

    Plasmid
    #225822
    Purpose
    Tn libraries Tn5 variant GEN backbone
    Depositor
    Nili Ostrov
    Type
    Empty backbone
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    N/A
    Promoter
    Available since
    Oct. 21, 2024
    Availability
    Academic Institutions and Nonprofits only
    225822-plasmid-map-sequence-id-449891

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  40. pGLU_130

    Plasmid
    #225823
    Purpose
    Tn libraries Tn5 variant ERM backbone
    Depositor
    Nili Ostrov
    Type
    Empty backbone
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    N/A
    Promoter
    Available since
    Oct. 21, 2024
    Availability
    Academic Institutions and Nonprofits only
    225823-plasmid-map-sequence-id-449890

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  41. pGLU_128

    Plasmid
    #225821
    Purpose
    Tn libraries Tn5 variant KAN backbone
    Depositor
    Nili Ostrov
    Type
    Empty backbone
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    N/A
    Promoter
    Available since
    Oct. 21, 2024
    Availability
    Academic Institutions and Nonprofits only
    225821-plasmid-map-sequence-id-449888

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  46. pGLU_116

    Plasmid
    #225796
    Purpose
    RS421 library variant 14271
    Depositor
    Nili Ostrov
    Insert
    14271_[Tn5]
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    N/A
    Promoter
    Available since
    Sept. 27, 2024
    Availability
    Academic Institutions and Nonprofits only
    225796-plasmid-map-sequence-id-447051

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  47. pGLU_109

    Plasmid
    #225790
    Purpose
    RS421 library variant 14073
    Depositor
    Nili Ostrov
    Insert
    14073_[Tn5]
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    N/A
    Promoter
    Available since
    Sept. 27, 2024
    Availability
    Academic Institutions and Nonprofits only
    225790-plasmid-map-sequence-id-447023

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  48. pGLU_108

    Plasmid
    #225789
    Purpose
    RS421 library variant 13166
    Depositor
    Nili Ostrov
    Insert
    13166_[Tn5]
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    N/A
    Promoter
    Available since
    Sept. 27, 2024
    Availability
    Academic Institutions and Nonprofits only
    225789-plasmid-map-sequence-id-447022

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  49. pGLU_107

    Plasmid
    #225788
    Purpose
    RS421 library variant 13168
    Depositor
    Nili Ostrov
    Insert
    13168_[Tn5]
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    N/A
    Promoter
    Available since
    Sept. 27, 2024
    Availability
    Academic Institutions and Nonprofits only
    225788-plasmid-map-sequence-id-446982

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  50. pGLU_118

    Plasmid
    #225798
    Purpose
    RS421 library variant 13550
    Depositor
    Nili Ostrov
    Insert
    13550_[Tn5]
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    N/A
    Promoter
    Available since
    Sept. 26, 2024
    Availability
    Academic Institutions and Nonprofits only
    225798-plasmid-map-sequence-id-446919

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Showing: 51 - 100 of 146 results