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Showing: 51 - 100 of 133 results

  3. pUBC-Pfv-Sapphire-IRES-H2B-mCherry

    Plasmid
    #203648
    Purpose
    Mammalian lentiviral vector for the expression of the Sapphire-tagged 40nm GEM under the UBC promoter, with a secondary translation initiation site for H2B-mCherry expression.
    Depositor
    Liam Holt
    Article
    Shu T et et al bioRxiv 2022
    Insert
    PfV
    Use
    Lentiviral
    Tags
    Sapphire-IRES-H2B-mCherry
    Expression
    Mutation
    Promoter
    Available since
    July 26, 2023
    Availability
    Academic Institutions and Nonprofits only
    203648-plasmid-map-sequence-id-402478

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  6. pX458-Dual-Guide-Donor-Cas9-H2B-mCherry

    Plasmid
    #175570
    Purpose
    Donor shuttle plasmid to enable ligation of a second U6-GuideRNA casssette into any pX458 family backbone via digestion of both plasmids with XbaI and KpnI.
    Depositor
    Joseph Brzezinski
    Type
    Empty backbone
    Use
    CRISPR
    Tags
    Expression
    Mutation
    XbaI site added upstream of U6 promotor. G to C …
    Promoter
    Available since
    Oct. 7, 2021
    Availability
    Academic Institutions and Nonprofits only
    175570-plasmid-map-sequence-id-344992

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  9. pUBC-SV40NLS-PfV-Sapphire-IRES-H2B-mCherry

    Plasmid
    #203652
    Purpose
    Mammalian lentiviral vector for the expression of the Sapphire-tagged nuclear localizing 40nm nucGEMs under the UBC promoter, with a secondary translation initiation site for H2B-mCherry expression.
    Depositor
    Liam Holt
    Article
    Shu T et et al bioRxiv 2022
    Insert
    PfV
    Use
    Lentiviral
    Tags
    Sapphire-IRES-H2B-mCherry
    Expression
    Mutation
    Promoter
    Available since
    July 26, 2023
    Availability
    Academic Institutions and Nonprofits only
    203652-plasmid-map-sequence-id-402483

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  11. pHR SFFVp MCP-mCherry IRES h2b-diRFP

    Plasmid
    #102351
    Purpose
    Lentiral expression vector bearing the MS2 Coat Protein (MCP) fused to mCherry as well as the nuclear marker histone 2b fused to two copies of irFP. Also bears the hygromycin selection marker.
    Depositor
    Jared Toettcher
    Article
    Wilson et al Mol Cell. 2017 Sep 7;67(5):757-769.e5. d
    Insert
    MS2 Coat Protein
    Use
    Lentiviral
    Tags
    mCherry
    Expression
    Mutation
    Promoter
    SFFV
    Available since
    Jan. 22, 2018
    Availability
    Academic Institutions and Nonprofits only
    102351-plasmid-map-sequence-id-197294

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  15. hEF1α-H2B-mVenus-IRES-mCherry PGK-Puromycin

    Plasmid
    #99278
    Purpose
    Dual fluorescent reporter - constitutive expression of mVenus (515/528) localized in the nucleus, mCherry (587/610) untargeted and visualized in the cytoplasm, and Puromycin resistance
    Depositor
    Jordan Miller
    Article
    Calderon et al Biomater Sci. 2017 Jun 29. doi: 10.1039/
    Inserts
    H2B-mVenus-IRES-mCherry
    Puro
    Use
    Lentiviral
    Tags
    H2B, IRES, PGK, mCherry, and mVenus
    Expression
    Mutation
    Promoter
    EF1 alpha-human and PGK-mouse
    Available since
    Nov. 13, 2017
    Availability
    Academic Institutions and Nonprofits only
    99278-plasmid-map-sequence-id-262659

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  23. pRRL-pEF-H2B-mCherry-T2A-rTetR-Dest-SV40

    Plasmid
    #186968
    Purpose
    Lentiviral destination vector for cloning desired protein as rTetR fusion for chromatin regulation reporter (pEF-H2B-mCherry-T2A-rTetR, with SV40) for expression in mammalian cells.
    Depositor
    Brian Liau
    Article
    Lue et al Nat Chem Biol. 2023 Feb;19(2):176-186. d
    Insert
    pEF-H2B-mCherry-T2A-rTetR-Dest-SV40
    Use
    Lentiviral
    Tags
    rTetR fusion
    Expression
    Mammalian
    Mutation
    Promoter
    EF-1alpha promoter
    Available since
    Oct. 14, 2022
    Availability
    Academic Institutions and Nonprofits only
    186968-plasmid-map-sequence-id-370475

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  24. Ath-HEN1::T2A::mCherry::TY1::H2B-pCFJ350-ttTi5605

    Plasmid
    #108362
    Purpose
    Expression of 2xFlag MYC tagged A.thaliana-HEN1 (Ath-HEN1, codon optimised for C. elegans) and mCherry under the promoter of choice in C. elegans
    Depositor
    Luisa Cochella
    Article
    Alberti et al Nat Methods. 2018 Feb 26. pii: nmeth.461
    Inserts
    Ath-HEN1 (HEN1 Codon optimised for C. elegans, Mustard Weed)
    mCherry
    his-15 (his-15 Nematode)
    unc-54 3' UTR
    Use
    Tags
    2xFlag, MYC, T2A peptide, and TY1 linker
    Expression
    Worm
    Mutation
    Promoter
    A rab-3:Ath-HEN1:unc-54 3' UTR was cloned as…
    Available since
    May 14, 2018
    Availability
    Academic Institutions and Nonprofits only
    108362-plasmid-map-sequence-id-209815

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  27. pRRL-pEF-H2B-mCherry-T2A-rTetR-DNMT3L-SV40

    Plasmid
    #186967
    Purpose
    Lentiviral vector encoding DNMT3A recruiter for methylation reporter (pEF-H2B-mCherry-T2A-rTetR-DNMT3L-SV40) for expression in mammalian cells.
    Depositor
    Brian Liau
    Article
    Lue et al Nat Chem Biol. 2023 Feb;19(2):176-186. d
    Insert
    DNMT3L (DNMT3L Synthetic, Human)
    Use
    Lentiviral
    Tags
    rTetR fusion
    Expression
    Mammalian
    Mutation
    Promoter
    EF-1alpha promoter
    Available since
    Oct. 14, 2022
    Availability
    Academic Institutions and Nonprofits only
    186967-plasmid-map-sequence-id-370462

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  32. pLV-pGK-Nluc-Fluc-NLS-P2A-H2B-mCherry-IRES-Hygro

    Plasmid
    #115684
    Purpose
    NanoLuc-Firefly Luciferase fusion with constitutive nuclear expression for calibration of Nanoluc signal to Firefly Luciferase signal. H2B-mCherry marks chromatin with red fluorescence.
    Depositor
    David Suter
    Article
    Mandic et al Methods. 2017 May 1;120:65-75. doi: 10.1
    Insert
    Nluc-Fluc-NLS-P2A-H2B-mCherry-IRES-Hygro
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Oct. 11, 2018
    Availability
    Academic Institutions and Nonprofits only
    115684-plasmid-map-sequence-id-224735

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  34. pUltra-hot-H2B-mTagBFP2

    Plasmid
    #161792
    Purpose
    3rd generation lentiviral multicistronic vector for mammalian expression of mCherry, H2B-mTagBFP2, and one gene of interest
    Depositor
    Jorge Luis Arias-Arias
    Insert
    H2B-mTagBFP2
    Use
    Lentiviral; Multicistronic
    Tags
    mCherry and mTagBFP2
    Expression
    Mammalian
    Mutation
    I174A in the mTagBFP2 protein according to PLoS O…
    Promoter
    UbC
    Available since
    Nov. 10, 2020
    Availability
    Academic Institutions and Nonprofits only
    161792-plasmid-map-sequence-id-316815

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Showing: 51 - 100 of 133 results