We narrowed to 6,627 results for: hwa

1. Glycogene CRISPR Library

   Pooled Library

   #140961

   Purpose

   Targets human genes involved in cellular glycosylation.

   Depositor

   Sriram Neelamegham

   Article

   Zhu et al Glycobiology. 2020 Aug 8. pii: 5889976.

   Species

   Homo sapiens

   Use

   CRISPR and Lentiviral

   Available since

   Sept. 9, 2020

   Availability

   Academic Institutions and Nonprofits only

2. Murine cell surface protein knockout (McspKO) library

   Pooled Library

   #200445

   Purpose

   A CRISPR library targeting 1,157 cell surface genes and 98 control genes with four sgRNAs per gene

   Depositor

   Junjie Chen

   Article

   Wang et al Proc Natl Acad Sci U S A. 2022 Jun 21;11

   Expression

   Mammalian

   Species

   Mus musculus

   Use

   CRISPR, Lentiviral, and Mouse Targeting

   Available since

   Oct. 26, 2023

   Availability

   Academic Institutions and Nonprofits only

3. Mouse sphingolipid metabolism knockout library

   Pooled Library

   #206249

   Purpose

   The sphingolipid metabolism library targets 82 mouse genes in the de novo ceramide and downstream sphingolipid metabolism pathways.

   Depositor

   Kivanc Birsoy

   Article

   Soula et al Nature. 2024 Aug 7. doi: 10.1038/s41586-

   Use

   CRISPR and Lentiviral

   Available since

   Aug. 29, 2024

   Availability

   Academic Institutions and Nonprofits only

4. mTORC1 Focused library

   Pooled Library

   #164084

   Purpose

   Lentiviral CRISPR library targets human mTORC1 signaling pathway

   Depositor

   David Sabatini

   Article

   Condon et al Proc Natl Acad Sci U S A. 2021 Jan 26;11

   Expression

   Mammalian

   Species

   Homo sapiens

   Use

   CRISPR and Lentiviral

   Available since

   Jan. 11, 2021

   Availability

   Academic Institutions and Nonprofits only

5. E. coli MEV20

   Bacterial Strain

   #197113

   Purpose

   E. coli BL21(DE3) derivative for diterpenoid production with genome-integrated yeast MEV pathway, redox enzyme array, and Marionette cluster

   Depositor

   Christopher Voigt

   Article

   Lin et al Nature Catalysis. 6, 1016–1029 (2023). h

   Bacterial Resistance

   None

   Available since

   June 30, 2023

   Availability

   Academic Institutions and Nonprofits only

6. E. coli MEV15

   Bacterial Strain

   #197112

   Purpose

   E. coli BL21(DE3) derivative for sesquiterpenoid production with genome-integrated yeast MEV pathway, redox enzyme array, and Marionette cluster

   Depositor

   Christopher Voigt

   Article

   Lin et al Nature Catalysis. 6, 1016–1029 (2023). h

   Bacterial Resistance

   None

   Available since

   June 30, 2023

   Availability

   Academic Institutions and Nonprofits only

7. CYN1 (E. coli Entner-Doudoroff v1)

   Bacterial Strain

   #71421

   Purpose

   This E. coli MG1655 strain has a genome-integrated genetic module containing a rationally engineered 5-enzyme Entner-Doudoroff pathway (first version). The module also contains a Cm resistance marker.

   Depositor

   Howard Salis

   Article

   Ng et al Metab Eng. 2015 May;29:86-96. doi: 10.10

   Bacterial Resistance

   Chloramphenicol

   Available since

   Feb. 18, 2016

   Availability

   Academic Institutions and Nonprofits only

8. CYN2/150 (E. coli Entner-Doudoroff v11)

   Bacterial Strain

   #71420

   Purpose

   This E. coli MG1655 strain has a genome-integrated genetic module containing a rationally engineered 5-enzyme Entner-Doudoroff pathway with an improved NADPH regeneration rate. Module is Cm resistant

   Depositor

   Howard Salis

   Article

   Ng et al Metab Eng. 2015 May;29:86-96. doi: 10.10

   Bacterial Resistance

   Chloramphenicol

   Available since

   Feb. 18, 2016

   Availability

   Academic Institutions and Nonprofits only

9. 2xFKBP-mCh-KDEL

   Plasmid

   #173010

   Purpose

   FKBP anchor for retaining DHFR-fused cargoes in the ER in the presence of zapalog. Encodes ER retrieval motif (KDEL) at C-terminus of mCherry. Contains IRES following ORF for cloning cargo.

   Depositor

   Matthew Kennedy

   Article

   Bourke et al J Cell Biol. 2021 Sep 6;220(9). pii: 212

   Insert

   2xFKBP-mCh-KDEL
   

   Use

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   Chicken beta actin (CAG)

   Available since

   Aug. 6, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. TfR-mCh-DHFR

    Plasmid

    #173013

    Purpose

    For light regulated release of transferrin receptor (TfR) from the endoplasmic reticulum. Driven by CMV promoter.

    Depositor

    Matthew Kennedy

    Article

    Bourke et al J Cell Biol. 2021 Sep 6;220(9). pii: 212

    Insert

    TfR-mCh-DHFR
    

    Use

    Tags

    DHFR and mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. DHFR-GFP-th-NL1

    Plasmid

    #173007

    Purpose

    For light regulated release of neuroligin 1 from the endoplasmic reticulum. Driven by synapsin promoter. Thrombin site is included between GFP and NL1.

    Depositor

    Matthew Kennedy

    Article

    Bourke et al J Cell Biol. 2021 Sep 6;220(9). pii: 212

    Insert

    DHFR-GFP-NL1
    

    Use

    Tags

    DHFR and EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    human synapsin

    Available since

    Aug. 5, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. DHFR-GFP-th-GluA1

    Plasmid

    #173008

    Purpose

    For light regulated release of GluA1 from the endoplasmic reticulum. Driven by synapsin promoter. Thrombin site is included between GFP and GluA1.

    Depositor

    Matthew Kennedy

    Article

    Bourke et al J Cell Biol. 2021 Sep 6;220(9). pii: 212

    Insert

    DHFR-GFP-th-GluA1
    

    Use

    Lentiviral

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    human synapsin

    Available since

    Aug. 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. DHFR-mNeon-GluA1

    Plasmid

    #173009

    Purpose

    For light regulated release of GluA1 from the endoplasmic reticulum. Driven by synapsin promoter. Intracellular trafficking can be better visualized with mNeon.

    Depositor

    Matthew Kennedy

    Article

    Bourke et al J Cell Biol. 2021 Sep 6;220(9). pii: 212

    Insert

    DHFR-mNeon-GluA1
    

    Use

    Tags

    DHFR and mNeon

    Expression

    Mammalian

    Mutation

    Promoter

    Chicken beta actin (CAG)

    Available since

    Aug. 5, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. TfR-moxGFP-DHFR

    Plasmid

    #173012

    Purpose

    For light regulated release of transferrin receptor (TfR) from the endoplasmic reticulum. Driven by CMV promoter. Fused to oxidation resistant version of GFP (moxGFP).

    Depositor

    Matthew Kennedy

    Article

    Bourke et al J Cell Biol. 2021 Sep 6;220(9). pii: 212

    Insert

    TfR-moxGFP-DHFR
    

    Use

    Tags

    DHFR and moxGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. 2xFKBP-smFP(myc)-KDEL

    Plasmid

    #173021

    Purpose

    FKBP anchor for retaining DHFR-fused cargoes in the ER in the presence of zapalog. Encodes ER retrieval motif (KDEL) at C-terminus of spaghetti monster FP (myc, non-fluorescent). IRES following ORF.

    Depositor

    Matthew Kennedy

    Article

    Bourke et al J Cell Biol. 2021 Sep 6;220(9). pii: 212

    Insert

    2xFKBP-smFP(myc)-KDEL
    

    Use

    Tags

    FKBP and smFP(myc) non-fluorescent

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. MK969

    Plasmid

    #71432

    Purpose

    The pAK plasmid expresses GFP driven by a T7 promoter

    Depositor

    Howard Salis

    Article

    Kushwaha et al Nat Commun. 2015 Jul 17;6:7832. doi: 10.

    Insert

    gfp
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    T7

    Available since

    Dec. 7, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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17. pHR_XPR2_hrGFP

    Plasmid

    #84614

    Purpose

    HR donor to integrate UAS1B8TEF(136)-hrGFP-CYC1t into XPR2 locus

    Depositor

    Ian Wheeldon

    Article

    Schwartz et al ACS Synth Biol. 2016 Dec 19.

    Insert

    hrGFP
    

    Use

    Synthetic Biology

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    UAS1B8-TEF(136)

    Available since

    Nov. 7, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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18. pHR_AXP_hrGFP

    Plasmid

    #84613

    Purpose

    HR donor to integrate UAS1B8TEF(136)-hrGFP-CYC1t into AXP locus

    Depositor

    Ian Wheeldon

    Article

    Schwartz et al ACS Synth Biol. 2016 Dec 19.

    Insert

    hrGFP
    

    Use

    Synthetic Biology

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    UAS1B8-TEF(136)

    Available since

    Nov. 7, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pHR_D17_hrGFP

    Plasmid

    #84616

    Purpose

    HR donor to integrate UAS1B8TEF(136)-hrGFP-CYC1t into D17 locus

    Depositor

    Ian Wheeldon

    Article

    Schwartz et al ACS Synth Biol. 2016 Dec 19.

    Insert

    hrGFP
    

    Use

    Synthetic Biology

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    UAS1B8-TEF(136)

    Available since

    Dec. 22, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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20. pHR_MFE1_hrGFP

    Plasmid

    #84617

    Purpose

    HR donor to integrate UAS1B8TEF(136)-hrGFP-CYC1t into MFE1 locus

    Depositor

    Ian Wheeldon

    Article

    Schwartz et al ACS Synth Biol. 2016 Dec 19.

    Insert

    hrGFP
    

    Use

    Synthetic Biology

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    UAS1B8-TEF(136)

    Available since

    Dec. 22, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge