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Showing: 951 - 1000 of 9523 results

  3. pEJS1084_pTetR-P2A-BFP/BspMI flexible sgRNA

    Plasmid
    #117687
    Purpose
    U6 driven Spy sgRNA cloning vector where guide sequences are inserted between BspMI sites. This plasmid works with Addgene #108570 for subnuclear proteomic profiling via C-BERST method.
    Depositor
    Erik Sontheimer
    Article
    Gao et al Methods Enzymol. 2019;616:365-383. doi:
    Inserts
    Kanamycin cassette
    TetR-P2A-BFP
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    U6 and hPGK
    Available since
    Dec. 17, 2018
    Availability
    Academic Institutions and Nonprofits only
    117687-plasmid-map-sequence-id-228342

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  11. pBsVchCAST-pyrE

    Plasmid
    #203813
    Purpose
    Expression of CRISPR-associated transposases, shuttle vector for Bacillus subtilis / E. coli
    Depositor
    Sheng Yang
    Article
    Yang et al ACS Synth Biol. 2023 Jul 21;12(7):2198-2
    Insert
    VchTniQ, VchCas5/8, VchCas7, VchCas6, VchTnsABC, CRISPR(pyrE)
    Use
    CRISPR
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Aug. 11, 2023
    Availability
    Academic Institutions and Nonprofits only
    203813-plasmid-map-sequence-id-403827

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  27. pCAGEN_NES_V5_GFP_FTA

    Plasmid
    #235633
    Purpose
    Encodes V5-GFP with an N-terminal nuclear export sequence and a C-terminal FLAG-TEV cleavage site-AviTag. Used to express a control substrate for ALIBi.
    Depositor
    Maria Barna
    Article
    Zhang et al Science. 2025 Mar 7;387(6738):eadn2623.
    Insert
    GFP
    Use
    Tags
    FLAG-TEV cleavage site-AviTag and NES-V5
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    April 2, 2025
    Availability
    Academic Institutions and Nonprofits only
    235633-plasmid-map-sequence-id-467518

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  28. pRB133.2

    Plasmid
    #181950
    Purpose
    aTc-inducible expression of PhoP with C-terminal mNeonGreen fusion. Also contains mCherry under PhoP-controlled promoter PvirK
    Depositor
    Jeffrey Tabor
    Article
    Butcher et al Proc Natl Acad Sci U S A. 2022 Aug 30;11
    Insert
    PhoP-Salmonella enterica subsp. enterica serovar Typhimurium (AX04_RS23485 PhoP-Salmonella enterica subsp. enterica serovar Typhimurium, Synthetic)
    Use
    Tags
    mNeonGreen
    Expression
    Bacterial
    Mutation
    Promoter
    PhoP-mNG-PLtetO-1; mCherry-PvirK; tetR-J23106
    Available since
    Oct. 26, 2022
    Availability
    Academic Institutions and Nonprofits only
    181950-plasmid-map-sequence-id-356579

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  32. KG#797

    Plasmid
    #110916
    Purpose
    Expresses the active zone - enriched protein Sentryn-GFP in a subset of cholinergic ventral nerve cord motor neurons
    Depositor
    Kenneth Miller
    Article
    Edwards et al Genetics. 2018 Nov;210(3):947-968. doi:
    Inserts
    unc-129 promoter
    STRN-1 (Sentryn)-GFP
    unc-54 3' control region with 1 intron just upstream and 1 intron in control region
    Use
    Tags
    GFP (contains 3 artificial introns)
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    March 11, 2021
    Availability
    Academic Institutions and Nonprofits only
    110916-plasmid-map-sequence-id-212580

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  34. pCR4-*mRFP

    Plasmid
    #47118
    Purpose
    Can be used to make an RNA probe for whole-mount in situ hybridization, for in-frame fusion proteins or as a subcloning step for mRFP. Note that the coding sequence for mRFP lacks the start codon.
    Depositor
    Stephen Ekker
    Article
    Clark et al Nat Methods. 2011 Jun;8(6):506-15. Epub
    Insert
    *mRFP
    Use
    Tags
    Expression
    Bacterial
    Mutation
    No start codon
    Promoter
    Lac
    Available since
    Oct. 16, 2013
    Availability
    Academic Institutions and Nonprofits only
    47118-plasmid-map-sequence-id-67205

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  50. pBiex PKC - Peptide 8 GFP RFP

    Plasmid
    #83564
    Purpose
    Expresses a GFP-RFP FRET sensor containing the catalytic domain of PKCalpha and a short peptide substrate derived from the EGF receptor.
    Depositor
    Sivaraj Sivaramakrishnan
    Article
    Sommese et al J Biol Chem. 2016 Aug 23. pii: jbc.M116.
    Insert
    PKC alpha (PRKCA Human)
    Use
    Tags
    10 nm ER/K Helix, FLAG purification tag, TEV Prot…
    Expression
    Bacterial and Insect
    Mutation
    Only catalytic domain of PKC (aa 335-672)
    Promoter
    T7 Promoter
    Available since
    Nov. 16, 2016
    Availability
    Academic Institutions and Nonprofits only
    83564-plasmid-map-sequence-id-166406

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Showing: 951 - 1000 of 9523 results