We narrowed to 9,897 results for: Tet-on

1. pES003 (pTet-qacR 2)

   Plasmid

   #69032

   Purpose

   qacR 2 downstream of a Tet promoter

   Depositor

   Richard Murray

   Article

   de Los Santos et al ACS Synth Biol. 2015 Aug 19.

   Insert

   qacR (qacR Synthetic)
   

   Tags

   His6 tag

   Expression

   Bacterial

   Mutation

   E57Q, E58L, W61Y, E90Q, I99Q, M116Q, L119Y, E120Q…

   Promoter

   pTet

   Available Since

   Sept. 25, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLenti-tetON-NKX2-1(delDBD)-EF1a-TagRFP-2A-tet3G

   Plasmid

   #204214

   Purpose

   Doxycycline inducible lentiviral vector for human NKX2-1 (delDBD) overexpression

   Depositor

   Emma Rawlins

   Article

   Lim et al Cell Stem Cell. 2023 Jan 5;30(1):20-37.e

   Insert

   NKX2-1 (NKX2-1 Human)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Mutation

   Deletion of DNA binding domain (delDBD)

   Promoter

   TetON

   Available Since

   Aug. 28, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLenti-tetON-NKX2-1-R162del-EF1a-TagRFP-2A-tet3G

   Plasmid

   #204191

   Purpose

   Doxycycline inducible lentiviral vector for human NKX2-1 (R162del) overexpression

   Depositor

   Emma Rawlins

   Article

   Lim et al Cell Stem Cell. 2023 Jan 5;30(1):20-37.e

   Insert

   NKX2-1 (NKX2-1 Human)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Mutation

   Deletion of R162

   Promoter

   TetON

   Available Since

   Aug. 28, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLenti-tetON-NKX2-1-I207M -EF1a-TagRFP-2A-tet3G

   Plasmid

   #204213

   Purpose

   Doxycycline inducible lentiviral vector for human NKX2-1 (I207M) overexpression

   Depositor

   Emma Rawlins

   Article

   Lim et al Cell Stem Cell. 2023 Jan 5;30(1):20-37.e

   Insert

   NKX2-1 (NKX2-1 Human)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Mutation

   Point mutation on I207M

   Promoter

   TetON

   Available Since

   Aug. 28, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLenti-tetON-NKX2-1-Q175∗-EF1a-TagRFP-2A-tet3G

   Plasmid

   #204211

   Purpose

   Doxycycline inducible lentiviral vector for human NKX2-1 (Q175∗) overexpression

   Depositor

   Emma Rawlins

   Article

   Lim et al Cell Stem Cell. 2023 Jan 5;30(1):20-37.e

   Insert

   NKX2-1 (NKX2-1 Human)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Mutation

   Point mutation on Q175* (*termination)

   Promoter

   TetON

   Available Since

   Aug. 28, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLenti-tetON-NKX2-1-R178∗-EF1a-TagRFP-2A-tet3G

   Plasmid

   #204210

   Purpose

   Doxycycline inducible lentiviral vector for human NKX2-1 (R178∗) overexpression

   Depositor

   Emma Rawlins

   Article

   Lim et al Cell Stem Cell. 2023 Jan 5;30(1):20-37.e

   Insert

   NKX2-1 (NKX2-1 Human)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Mutation

   Point mutation on R178* (*termination)

   Promoter

   TetON

   Available Since

   Aug. 28, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLenti-tetON-NKX2-1-I207F -EF1a-TagRFP-2A-tet3G

   Plasmid

   #204212

   Purpose

   Doxycycline inducible lentiviral vector for human NKX2-1 (I207F) overexpression

   Depositor

   Emma Rawlins

   Article

   Lim et al Cell Stem Cell. 2023 Jan 5;30(1):20-37.e

   Insert

   NK2 homeobox 1 (NKX2-1 Human)
   

   Use

   Lentiviral

   Tags

   Point mutation on I207F

   Expression

   Mammalian

   Promoter

   TetON

   Available Since

   Aug. 28, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pBEST-Pl-tetO1-tetR-linker-deGFP-T500 (109p)

   Plasmid

   #70195

   Purpose

   Post cloned 4-piece from Isothermal/Golden Gate assembly (GGA), using sequences from 2 (PltetO1-UTR1), 3 (tetR+linker from primer extension), 21 (linker from primer extension + deGFP), and 105 (vector

   Depositor

   Richard Murray

   Article

   Sun et al ACS Synth Biol. 2014 Jun 20;3(6):387-97.

   Insert

   tetR-deGFP fusion
   

   Available Since

   May 19, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. pLenti-tetON-KRAB-dCas9-DHFR-EF1a-TagRFP-2A-tet3G

   Plasmid

   #167935

   Purpose

   Inducible CRISPRi expression

   Depositor

   Emma Rawlins

   Article

   Sun et al eLife. 2021 Oct 6;10. pii: 67886. doi: 1

   Insert

   KRAB-dCas9-DHFR
   

   Use

   Lentiviral

   Promoter

   tetON

   Available Since

   June 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pRS416-dCas9-Mxi1 + TetR + pRPR1(TetO)-NotI-gRNA

    Plasmid

    #73796

    Purpose

    pRS416 Ura marked Cen/Ars plasmid with dCas9-Mxi1 under Tef1 promoter, and tet-incucibile RPR1 promoter with NotI cloning site adjacent to gRNA

    Depositor

    Ronald Davis

    Article

    Smith et al Genome Biol. 2016 Mar 8;17(1):45. doi: 1

    Inserts

    dCas9-Mxi1
    Tet Repressor
    Structural gRNA for S pyogenes
    

    Use

    CRISPR

    Tags

    Mxi1 and NLS

    Expression

    Yeast

    Mutation

    D10A, H840A

    Promoter

    pGPM1, pRPR1(TetO), and pTef1

    Available Since

    March 10, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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11. pLenti-tetON-dxCas9(3.7)-VPR-EF1a-TagRFP-2A-tet3G

    Plasmid

    #167937

    Purpose

    Inducible CRISPRa expression

    Depositor

    Emma Rawlins

    Article

    Sun et al eLife. 2021 Oct 6;10. pii: 67886. doi: 1

    Insert

    dxCas9(3.7)-VPR
    

    Use

    Lentiviral

    Promoter

    tetON

    Available Since

    June 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. PB-TET

    Plasmid

    #20909

    Purpose

    piggyBac empty vector with Gateway cassette, tet inducible promoter, and linked reporter (IRES-bgeo)

    Depositor

    Andras Nagy

    Article

    Woltjen et al Nature. 2009 Apr 9;458(7239):766-70. doi

    Insert

    Gateway Destination Vector
    

    Use

    piggybac

    Tags

    IRES-bGeo

    Expression

    Mammalian

    Available Since

    May 5, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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13. pLenti-Tet

    Plasmid

    #138086

    Purpose

    Empty vector with Tet-response promoter for inducible expression of proteins. Can be transduced using Lentiviral particles

    Depositor

    Eva Frickel

    Article

    Fisch et al EMBO J. 2019 Jul 1;38(13):e100926. doi:

    Type

    Empty backbone

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    minimal CMV promoter with heptamerized tet‐operat…

    Available Since

    March 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. tet-pLKO.puro_shGFP

    Plasmid

    #110421

    Purpose

    shGFP control, silence GFP gene, doxycycline inducible, puromycin selection

    Depositor

    Sandra Martha Gomes Dias

    Insert

    GFP
    

    Use

    Lentiviral and RNAi

    Expression

    Mammalian

    Promoter

    H1/TO (RNA PolIII)

    Available Since

    June 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pGDP4 tet(A)

    Plasmid

    #112887

    Purpose

    This plasmid is part of the Minimal Antibiotic Resistance Platform (ARP) Kit (Addgene #1000000143). Low copy-number plasmid that constitutively expresses genes using the Plac promoter.

    Depositor

    Gerard Wright

    Article

    Cox et al Cell Chem Biol. 2017 Jan 19;24(1):98-109

    Insert

    tet(A)
    

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Sequence was codon optimized for E. coli K12 usin…

    Promoter

    Plac

    Available Since

    Dec. 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. tet-pLKO.puro_shLuc

    Plasmid

    #110422

    Purpose

    shLuc control, silence Luc gene, doxycycline inducible, puromycin selection

    Depositor

    Sandra Martha Gomes Dias

    Insert

    Luciferase
    

    Use

    Lentiviral and RNAi

    Expression

    Mammalian

    Promoter

    H1/TO (RNA PolIII)

    Available Since

    June 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. tet-pLKO.neo_shGFP

    Plasmid

    #110470

    Purpose

    Control, silence GFP gene, doxycycline inducible, neomycin selection

    Depositor

    Sandra Martha Gomes Dias

    Article

    Reis et al J Biol Chem. 2019 Jun 14;294(24):9342-93

    Insert

    GFP
    

    Use

    Lentiviral and RNAi

    Expression

    Mammalian

    Promoter

    H1/TO (RNA PolIII)

    Available Since

    Feb. 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. tet-pLKO.puro_shGAC

    Plasmid

    #110423

    Purpose

    shGAC, silence glutaminase GAC isoform, doxycycline inducible, puromycin selection

    Depositor

    Sandra Martha Gomes Dias

    Insert

    GLS glutaminase (GLS Human)
    

    Use

    Lentiviral and RNAi

    Expression

    Mammalian

    Promoter

    H1/TO (RNA PolIII)

    Available Since

    June 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. tet operator

    Plasmid

    #8901

    Depositor

    Lynda Chin

    Article

    Chin et al Nature. 1999 Jul 29. 400(6743):468-72.

    Insert

    tet operator
    

    Available Since

    Jan. 5, 2006

    Availability

    Academic Institutions and Nonprofits only

20. Tet-pLKO-puro-Scrambled

    Plasmid

    #47541

    Purpose

    Tet inducible scrambled shRNA.

    Depositor

    Charles Rudin

    Article

    Rudin et al Nat Genet. 2012 Oct;44(10):1111-6. doi:

    Insert

    Scrambled shRNA
    

    Use

    Lentiviral and RNAi

    Expression

    Mammalian

    Promoter

    H1

    Available Since

    April 28, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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