Skip to main content
Addgene

Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

Information

In Category

Filter By

We narrowed to 17,912 results for: REV

Showing: 9951 - 10000 of 17912 results

  14. pET15b I-SceI (Ctermhis) K223I

    Plasmid
    #195615
    Purpose
    Expresses I-SceI homing endonuclease from the T7 promoter containing an additional methionine-histidine sequence at the amino terminus and a K223I mutation that results in nicking activity
    Depositor
    Frederick Gimble
    Article
    Niu et al J Mol Biol. 2008 Sep 26;382(1):188-202.
    Insert
    I-SceI
    Use
    Tags
    Expression
    Bacterial
    Mutation
    C2694G, G2345C, G2351C, A3365T, A3366T
    Promoter
    T7
    Available since
    Feb. 1, 2023
    Availability
    Academic Institutions and Nonprofits only
    195615-plasmid-map-sequence-id-383680

    Enlarge

  15. pET15b I-SceI (Ctermhis) K122I

    Plasmid
    #195614
    Purpose
    Expresses I-SceI homing endonuclease from the T7 promoter containing an additional methionine-histidine sequence at the amino terminus and a K122I mutation that results in nicking activity
    Depositor
    Frederick Gimble
    Article
    Niu et al J Mol Biol. 2008 Sep 26;382(1):188-202.
    Insert
    I-SceI
    Use
    Tags
    Expression
    Bacterial
    Mutation
    C2458G, A2826T, A2827T
    Promoter
    T7
    Available since
    Feb. 1, 2023
    Availability
    Academic Institutions and Nonprofits only
    195614-plasmid-map-sequence-id-383676

    Enlarge

  17. KAN(G418)-TET-OFF – ubiquitin – LEU – 4MYC

    Plasmid
    #193318
    Purpose
    Doxycycline-induced transcriptional regulation and Ubiquitin - LEU - 4xMYC N-terminal tagging. Ubiquitin is cleaved off, resulting in an unstable protein with an N-terminal leucine.
    Depositor
    Rob Mitra
    Article
    Shively et al ACS Synth Biol. 2023 Jan 17. doi: 10.102
    Insert
    Ubiquitin - Leucine - 4MYC N-terminal tagging
    Use
    Tags
    4MYC
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Feb. 1, 2023
    Availability
    Academic Institutions and Nonprofits only
    193318-plasmid-map-sequence-id-383635

    Enlarge

  18. KAN(G418)-TET-OFF – ubiquitin – LEU – 4FLAG

    Plasmid
    #193317
    Purpose
    Doxycycline-induced transcriptional regulation and Ubiquitin - LEU - 4xFLAG N-terminal tagging. Ubiquitin is cleaved off, resulting in an unstable protein with an N-terminal leucine.
    Depositor
    Rob Mitra
    Article
    Shively et al ACS Synth Biol. 2023 Jan 17. doi: 10.102
    Insert
    Ubiquitin - Leucine - 4FLAG N-terminal tagging
    Use
    Tags
    4FLAG
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Feb. 1, 2023
    Availability
    Academic Institutions and Nonprofits only
    193317-plasmid-map-sequence-id-383630

    Enlarge

  19. pET PI-SceI C-His

    Plasmid
    #195610
    Purpose
    Expresses PI-SceI (VDE) homing endonuclease/intein from the T7 promoter with a carboxyl-terminal histidine tag
    Depositor
    Frederick Gimble
    Article
    He et al J Biol Chem. 1998 Feb 20;273(8):4607-15.
    Insert
    PI-SceI
    Use
    Tags
    Expression
    Bacterial
    Mutation
    T825C, C900G, A901C, A903C, T1131G, T1137C, T1227…
    Promoter
    T7
    Available since
    Jan. 31, 2023
    Availability
    Academic Institutions and Nonprofits only
    195610-plasmid-map-sequence-id-383258

    Enlarge

  37. Linked Free NES (M)

    Plasmid
    #182436
    Purpose
    Yeast integrative plasmid for expressing fusion protein ERG20(F96W-N127W)-AcNES1 (GAL10 promoter). Contains uracil auxotrophic marker (K. lactis URA3).
    Depositor
    Claudia Vickers
    Article
    Cheah et al bioRxiv 2022.11.24.517869
    Insert
    FPPS(M)-NES
    Use
    Synthetic Biology; Metabolic engineering
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    GAL10
    Available since
    Dec. 6, 2022
    Availability
    Academic Institutions and Nonprofits only
    182436-plasmid-map-sequence-id-360120

    Enlarge

  38. Coexpressed Free NES (M)

    Plasmid
    #182437
    Purpose
    Yeast integrative plasmid for expressing ERG20(F96W-N127W) (GAL10 promoter) and AcNES1 (GAL7 promoter). Contains uracil auxotrophic marker (K. lactis URA3).
    Depositor
    Claudia Vickers
    Article
    Cheah et al bioRxiv 2022.11.24.517869
    Inserts
    FPPS(M)
    NES
    Use
    Synthetic Biology; Metabolic engineering
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    GAL10 and GAL7
    Available since
    Dec. 6, 2022
    Availability
    Academic Institutions and Nonprofits only
    182437-plasmid-map-sequence-id-360121

    Enlarge

Showing: 9951 - 10000 of 17912 results