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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 22,814 results for: Emb;

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Showing: 10001 - 10050 of 22814 results

  1. pCDNA3_altPKA-Cα-mPAGFP

    Plasmid
    #114133
    Purpose
    Alternative spliced variant of the mouse PKA catalytic subunit α C-terminally tagged by monomeric paGFP.
    Depositor
    Haining Zhong
    Article
    Tillo et al Cell Rep. 2017 Apr 18;19(3):617-629. doi
    Insert
    Testis only splice variant of mouse PKA catalytic subunit α that has no myristoylation site, C-terminally tagged by mPAGFP (Prkaca Mouse)
    Expression
    Mammalian
    Available Since
    Oct. 24, 2018
    Availability
    Academic Institutions and Nonprofits only
    114133-plasmid-map-sequence-id-225608

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  9. GPRC5A-DuET

    Plasmid
    #213295
    Purpose
    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.
    Depositor
    Thomas Sakmar
    Article
    Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:
    Insert
    GPRC5A (GPRC5A Human)
    Tags
    1D4 and FLAG
    Expression
    Mammalian
    Promoter
    CMV, T7
    Available Since
    April 23, 2024
    Availability
    Academic Institutions and Nonprofits only
    213295-plasmid-map-sequence-id-430839

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  13. pLenti GW V5 Eco/Dam mse EMD

    Plasmid
    #182675
    Purpose
    Lentiviral eukaryotic expression vector with E.Coli Dam methylation fused to mouse Emerin. Used in DamID experiments to methylate DNA that interacts in the proximity of Emerin.
    Depositor
    Bas van Steensel
    Article
    Amendola et al EMBO Rep. 2015 May;16(5):610-7. doi: 10.
    Insert
    Emerin (Emd Mouse)
    Use
    Lentiviral
    Tags
    E. Coli Dam and V5
    Expression
    Mammalian
    Promoter
    HSP
    Available Since
    April 22, 2022
    Availability
    Academic Institutions and Nonprofits only
    182675-plasmid-map-sequence-id-358621

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  22. pUC19-E6I4Q5

    Plasmid
    #103101
    Purpose
    Cas9 coding gene template with optimized sequence for human codon usage
    Depositor
    Duhee Bang
    Article
    Cho et al Nucleic Acids Res. 2018 May 18;46(9):e55
    Insert
    E6I4Q5(Cas9 coding gene from Enterococcus faecalis TX0012)
    Use
    CRISPR; Cloning vector
    Mutation
    human codon-optimized
    Available Since
    April 20, 2018
    Availability
    Academic Institutions and Nonprofits only
    103101-plasmid-map-sequence-id-201810

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  23. pUC19-F3UXG6

    Plasmid
    #103108
    Purpose
    Cas9 coding gene template with optimized sequence for human codon usage
    Depositor
    Duhee Bang
    Article
    Cho et al Nucleic Acids Res. 2018 May 18;46(9):e55
    Insert
    F3UXG6(Cas9 coding gene from Streptococcus sanguinis SK49)
    Use
    CRISPR; Cloning vector
    Mutation
    human codon-optimized
    Available Since
    April 20, 2018
    Availability
    Academic Institutions and Nonprofits only
    103108-plasmid-map-sequence-id-201817

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  35. KIF1C-FHA

    Plasmid
    #25549
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    KIF1C-FHA (KIF1C Human)
    Tags
    His
    Expression
    Bacterial
    Mutation
    FHA domain only
    Available Since
    Aug. 25, 2010
    Availability
    Industry, Academic Institutions, and Nonprofits

  37. pDEST14-DoubleCatcher-⍺-Lock

    Plasmid
    #216286
    Purpose
    Expresses DoubleCatcher-⍺-Lock in bacterial cells. DoubleCatcher-⍺-Lock is a variant of DoubleCatcher in which the orientation of each SpyCatcher003 moiety is locked via an internal disulfide bond.
    Depositor
    Mark Howarth
    Article
    Driscoll et al Nat Commun. 2024 Mar 16;15(1):2403. doi:
    Insert
    DoubleCatcher ⍺-Lock
    Tags
    His6 and TEV cleavage site
    Expression
    Bacterial
    Promoter
    T7 promoter
    Available Since
    June 26, 2024
    Availability
    Academic Institutions and Nonprofits only
    216286-plasmid-map-sequence-id-433335

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  38. pDEST14-DoubleCatcher-β-Lock

    Plasmid
    #216287
    Purpose
    Expresses DoubleCatcher-β-Lock in bacterial cells. DoubleCatcher-β-Lock is a variant of DoubleCatcher in which the orientation of each SpyCatcher003 moiety is locked via an internal disulfide bond.
    Depositor
    Mark Howarth
    Article
    Driscoll et al Nat Commun. 2024 Mar 16;15(1):2403. doi:
    Insert
    DoubleCatcher β-Lock
    Tags
    His6 and TEV cleavage site
    Expression
    Bacterial
    Promoter
    T7 promoter
    Available Since
    June 26, 2024
    Availability
    Academic Institutions and Nonprofits only
    216287-plasmid-map-sequence-id-433337

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  39. pDEST14-DoubleCatcher-γ-Lock

    Plasmid
    #216288
    Purpose
    Expresses DoubleCatcher-γ-Lock in bacterial cells. DoubleCatcher-γ-Lock is a variant of DoubleCatcher in which the orientation of each SpyCatcher003 moiety is locked via an internal disulfide bond.
    Depositor
    Mark Howarth
    Article
    Driscoll et al Nat Commun. 2024 Mar 16;15(1):2403. doi:
    Insert
    DoubleCatcher γ-Lock
    Tags
    His6 and TEV cleavage site
    Expression
    Bacterial
    Promoter
    T7 promoter
    Available Since
    June 26, 2024
    Availability
    Academic Institutions and Nonprofits only
    216288-plasmid-map-sequence-id-433338

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  40. pDEST14-DoubleCatcher-δ-Lock

    Plasmid
    #216289
    Purpose
    Expresses DoubleCatcher-δ-Lock in bacterial cells. DoubleCatcher-δ-Lock is a variant of DoubleCatcher in which the orientation of each SpyCatcher003 moiety is locked via an internal disulfide bond.
    Depositor
    Mark Howarth
    Article
    Driscoll et al Nat Commun. 2024 Mar 16;15(1):2403. doi:
    Insert
    DoubleCatcher δ-Lock
    Tags
    His6 and TEV cleavage site
    Expression
    Bacterial
    Promoter
    T7 promoter
    Available Since
    June 26, 2024
    Availability
    Academic Institutions and Nonprofits only
    216289-plasmid-map-sequence-id-433340

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  41. pDEST14-DoubleCatcher-ε-Lock

    Plasmid
    #216290
    Purpose
    Expresses DoubleCatcher-ε-Lock in bacterial cells. DoubleCatcher-ε-Lock is a variant of DoubleCatcher in which the orientation of each SpyCatcher003 moiety is locked via an internal disulfide bond.
    Depositor
    Mark Howarth
    Article
    Driscoll et al Nat Commun. 2024 Mar 16;15(1):2403. doi:
    Insert
    DoubleCatcher ε-Lock
    Tags
    His6 and TEV cleavage site
    Expression
    Bacterial
    Promoter
    T7 promoter
    Available Since
    June 26, 2024
    Availability
    Academic Institutions and Nonprofits only
    216290-plasmid-map-sequence-id-433359

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  47. pUPD2 GFP-NT (GB2236)

    Plasmid
    #160590
    Purpose
    GFP for N-terminal fusions
    Depositor
    Diego Orzaez
    Insert
    GFP-NT
    Use
    Synthetic Biology
    Mutation
    BsaI and BsmBI sites removed
    Available Since
    Jan. 14, 2021
    Availability
    Academic Institutions and Nonprofits only
    160590-plasmid-map-sequence-id-322076

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  48. pUPD2 GFP-CT (GB2238)

    Plasmid
    #160592
    Purpose
    GFP for C-terminal fusions.
    Depositor
    Diego Orzaez
    Insert
    GFP-CT
    Use
    Synthetic Biology
    Mutation
    BsaI and BsmBI sites removed
    Available Since
    Jan. 14, 2021
    Availability
    Academic Institutions and Nonprofits only
    160592-plasmid-map-sequence-id-322074

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Showing: 10001 - 10050 of 22814 results