167,825 results

1. phage-cmv-cfp-24xms2

   Plasmid

   #40651

   Depositor

   Robert Singer

   Article

   Wu et al Biophys J. 2012 Jun 20;102(12):2936-44.

   Insert

   CFP-24xMBS
   

   Use

   Lentiviral

   Tags

   CFP

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Oct. 18, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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2. pMK436 (mAID-mCherry-P2A-Hygro)

   Plasmid

   #168231

   Purpose

   C-terminal tagging with mAID-mCherry

   Depositor

   Masato Kanemaki

   Article

   Saito et al Curr Protoc. 2021 Aug;1(8):e219. doi: 10

   Insert

   mAID-mCherry-P2A-Hygro
   

   Use

   CRISPR

   Expression

   Mammalian

   Available Since

   Oct. 21, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pOSCAR

   Plasmid

   #29639

   Depositor

   David_L Andrews, Scott Gold

   Article

   Paz et al Fungal Genet Biol. 2011 Jul;48(7):677-84

   Type

   Empty backbone

   Available Since

   Sept. 19, 2011

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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4. pSYC-437

   Plasmid

   #163829

   Purpose

   Foxj1a:GCaMP6s. Fluorescent reporter for calcium signaling (GCaMP6s) driven by zebrafish foxj1a promoter.

   Depositor

   Seok-Yong Choi

   Article

   Zhang et al Nat Commun. 2020 Apr 20;11(1):1860. doi:

   Inserts

   Foxj1a Promoter
   GCaMP6s
   

   Use

   Multisite gateway destination vector

   Available Since

   Feb. 5, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. LV-RFP

   Plasmid

   #26001

   Depositor

   Elaine Fuchs

   Article

   Beronja et al Nat Med. 2010 Jul . 16(7):821-7.

   Insert

   H2B-RFP (H2BC21 Human, A. victoria)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Available Since

   Dec. 10, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCAIP-hXRCC1pd-eGFP

   Plasmid

   #206033

   Purpose

   Mammalian expression of PAR-deficient hXRCC1pd coupled to eGFP under the control of a CAG promoter

   Depositor

   Primo Schaer

   Article

   Schwarz et al Nat Commun. 2024 Jan 2;15(1):184. doi: 1

   Insert

   hXRCC1
   

   Tags

   eGFP

   Expression

   Mammalian

   Mutation

   S103A,R186A,S184A,S193A,S219A,S220A,S236A,S268A

   Promoter

   CAG

   Available Since

   Jan. 3, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pPB CAG SMARCA4-IRES-EGFP

   Plasmid

   #153948

   Purpose

   piggyBac transposon vector with CAG promoter expressing wild-type SMARCA4 (FLAG-tagged) and EGFP

   Depositor

   Luca Tiberi

   Article

   Ballabio et al Nat Commun. 2020 Jan 29;11(1):583. doi:

   Insert

   SMARCA4 (SMARCA4 Human)
   

   Tags

   FLAG

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   Aug. 26, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAHYB-GFP

   Plasmid

   #85074

   Purpose

   Expression of GFP in Pichia pastoris

   Depositor

   Sheng Yang

   Article

   Yang et al Yeast. 2014 Apr;31(4):115-25.

   Insert

   GFP
   

   Expression

   Yeast

   Promoter

   AOX1

   Available Since

   March 17, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pFB2

   Plasmid

   #195282

   Purpose

   Bicistronic DTR and Puromycin resistance for positive/negative selection

   Depositor

   Scott Lowe

   Article

   Barriga et al Nat Cancer. 2022 Nov;3(11):1367-1385. do

   Insert

   DTR 2A Puro (HBEGF Human, Synthetic)
   

   Expression

   Mammalian

   Promoter

   PGK

   Available Since

   Jan. 26, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pet30b-5M SrtA

    Plasmid

    #51140

    Purpose

    expression plasmid for C-terminal his tagged S. aureus SrtA with enhanced catalytic activity

    Depositor

    Hidde Ploegh

    Article

    Shi et al Proc Natl Acad Sci U S A. 2014 Jul 15;11

    Insert

    S.aureus SrtA 5M
    

    Tags

    6x His

    Expression

    Bacterial

    Mutation

    P94R, D160N, D165A, K190E, K196T

    Promoter

    T7

    Available Since

    Feb. 24, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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11. pORTMAGE-2

    Plasmid

    #72677

    Purpose

    Expresses Lambda Red recombinases and a dominant negative MutL allele all controlled by temperature sensitve cI857 repressor for high precision and efficiency MAGE experiments. Ap resistance marker.

    Depositor

    Csaba Pál

    Article

    Nyerges et al Proc Natl Acad Sci U S A. 2016 Feb 16. p

    Insert

    mutL E32K
    

    Tags

    none

    Expression

    Bacterial

    Mutation

    E32K mutation conferring dominant mutator phenoty…

    Promoter

    pL promoter

    Available Since

    Feb. 18, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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12. pTF-ahpC-rfp

    Plasmid

    #153035

    Purpose

    For CRISPR-Cas12a genome editing in E. coli. Contains a constituitively expressed CRISPR array targeting the ahpC gene in E. coli and donor DNA to insert an rfp translational fusion in the ahpC gene.

    Depositor

    Nigel Scrutton

    Article

    Jervis et al Microb Biotechnol. 2021 Mar 12. doi: 10.

    Insert

    CRISPR array
    

    Expression

    Bacterial

    Available Since

    May 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pHR_PGK

    Plasmid

    #79120

    Purpose

    Lentiviral vector for constitutive expression of genes (PGK promoter)

    Depositor

    Wendell Lim

    Article

    Morsut et al Cell. 2016 Feb 11;164(4):780-91. doi: 10

    Type

    Empty backbone

    Use

    Lentiviral

    Available Since

    July 26, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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14. pSC142

    Plasmid

    #122835

    Purpose

    SUC2 promoter with GreenGate A-B flanking sequences

    Depositor

    Markus Schmid

    Article

    Lee et al PLoS One. 2019 Sep 26;14(9):e0222778. do

    Insert

    SUC2 promoter
    

    Use

    Golden gate compatible cloning vector

    Available Since

    March 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pScPPX2

    Plasmid

    #112877

    Purpose

    Expresses His-tagged yeast exopolyphosphatase for purification from E. coli

    Depositor

    Michael Gray, Ursula Jakob

    Article

    Gray et al Mol Cell. 2014 Mar 6;53(5):689-99. doi:

    Insert

    PPX1 (PPX1 Budding Yeast)
    

    Tags

    6X His tag

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Aug. 23, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. AAV2/9 REP-AAP-ΔCAP

    Plasmid

    #205991

    Purpose

    Rep and AAP protein expression for AAV CREATE libraries

    Depositor

    Viviana Gradinaru

    Article

    Chuapoco et al Nat Nanotechnol. 2023 Jul 10. doi: 10.10

    Insert

    AAV9 Rep and AAP proteins for CREATE libraries
    

    Use

    AAV

    Available Since

    Aug. 18, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. mscv2.2-6mycFlaA

    Plasmid

    #60203

    Purpose

    6xMYC-tagged full-length FlaA from Legionella pneumophila, expressed under the constitutive, moderate-level retroviral LTR promoter for mammalian cell expression; IRES-GFP reporter downstream of FlaA

    Depositor

    Russell Vance

    Article

    Kofoed et al Nature. 2011 Aug 28;477(7366):592-5. doi

    Insert

    FlaA
    

    Use

    Retroviral

    Tags

    6xMYC

    Expression

    Mammalian

    Available Since

    Feb. 11, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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18. pAAV.CMV.PI.EGFP.WPRE.bGH (AAV8)

    Viral Prep

    #105530-AAV8

    Purpose

    Ready-to-use AAV8 particles produced from pAAV.CMV.PI.EGFP.WPRE.bGH (#105530). In addition to the viral particles, you will also receive purified pAAV.CMV.PI.EGFP.WPRE.bGH plasmid DNA. CMV-driven EGFP control. These AAV preparations are suitable purity for injection into animals.

    Depositor

    James M. Wilson

    Promoter

    CMV

    Tags

    EGFP

    Available Since

    June 29, 2018

    Availability

    Academic Institutions and Nonprofits only

19. pCMV-lyso-pHluorin

    Plasmid

    #70113

    Purpose

    Expression of lyso-pHluorin in mammalian cells for optical readout of lysosomal acidification

    Depositor

    Christian Rosenmund

    Article

    Rost et al Nat Neurosci. 2015 Nov 9. doi: 10.1038/n

    Insert

    lyso-pHluorin (Cd63 Mouse, Aequorea victoria)
    

    Tags

    HA-Tag and pHluorin

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Nov. 11, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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20. pSPObooster

    Plasmid

    #216160

    Purpose

    Plasmid expressing the RME1(ins-308A) and MKT1(30G) genes from S. cerevisiae to correct sporulation defect of S288C derived yeast lab strains

    Depositor

    Grant Brown

    Article

    Loll-Krippleber et al Yeast. 2024 Sep 9. doi: 10.1002/yea.3978

    Inserts

    MKT1(30G) (MKT1 Budding Yeast)
    RME1(ins-308A) (RME1 Budding Yeast)
    

    Expression

    Yeast

    Mutation

    MKT1 gene containing an aspartic acid to glycine …

    Promoter

    endogenous promoter

    Available Since

    May 30, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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21. Anep8_Cas9

    Plasmid

    #117169

    Purpose

    Expressed Cas9 in Aspergillus with LIC tags for easy gRNA cloning

    Depositor

    Adrian Tsang

    Article

    Song et al PLoS One. 2018 Aug 24;13(8):e0202868. do

    Type

    Empty backbone

    Use

    CRISPR and Synthetic Biology; Filamentous fungi e…

    Promoter

    Pki

    Available Since

    Jan. 29, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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22. LTN1_pCSdest

    Plasmid

    #53855

    Depositor

    Roger Reeves

    Article

    Edie et al G3 (Bethesda). 2018 Jul 2;8(7):2215-2223

    Insert

    LTN1 (LTN1 Human)
    

    Expression

    Mammalian

    Promoter

    SP6 and CMV

    Available Since

    Sept. 9, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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23. pTlpA-Wasabi

    Plasmid

    #85455

    Purpose

    mWasabi reporter gene gated by wild type TlpA

    Depositor

    Mikhail Shapiro

    Article

    Piraner et al Nat Chem Biol. 2017 Jan;13(1):75-80. doi

    Inserts

    TlpA
    mWasabi
    

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    D341A

    Promoter

    TlpA and TlpA, LacI

    Available Since

    Feb. 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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24. pLenti CMV/TO Hygro empty (w214-1)

    Plasmid

    #17484

    Purpose

    3rd gen lentiviral empty control vector, CMV/TO promoter, Hygro 

    Depositor

    Paul Kaufman, Eric Campeau

    Article

    Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

    Type

    Empty backbone

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    Aug. 12, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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25. pAAV-CA

    Plasmid

    #69616

    Purpose

    To express gene strongly with AAV

    Depositor

    Naoshige Uchida

    Article

    Menegas et al Elife. 2015 Aug 31;4. doi: 10.7554/eLife

    Type

    Empty backbone

    Use

    AAV and Cre/Lox

    Expression

    Mammalian

    Available Since

    Oct. 6, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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26. pCggRO-reporter

    Plasmid

    #124582

    Purpose

    Reporter plasmid with Constitutively expressed mCherry and CggR-regulated YFP (eCitrine)

    Depositor

    Matthias Heinemann

    Article

    Monteiro et al Mol Syst Biol. 2019 Dec;15(12):e9071. do

    Insert

    CggR promoter element
    

    Expression

    Yeast

    Available Since

    June 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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27. GAL2-Tango

    Plasmid

    #66289

    Purpose

    Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation

    Depositor

    Bryan Roth

    Article

    Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-

    Insert

    GAL2 (GALR2 Human)
    

    Tags

    FLAG

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 15, 2015

    Availability

    Academic Institutions and Nonprofits only

28. pLENTI_TetOn_Hu_BIRC5(WT)

    Plasmid

    #136348

    Purpose

    Lentiviral inducible expression (TetOn) of human BIRC5

    Depositor

    Guillermo de Cárcer

    Insert

    BIRC5 (BIRC5 Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    Feb. 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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29. pDEST-EGFP-TARG1

    Plasmid

    #172586

    Purpose

    For transient expression of N-terminal GFP-tagged TARG1

    Depositor

    Karla Feijs

    Article

    Zaja et al Sci Rep. 2020 May 19;10(1):8286. doi: 10

    Insert

    TARG1 (OARD1 Human)
    

    Tags

    GFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    April 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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30. pJH001

    Plasmid

    #67641

    Purpose

    High copy Cas9 expression vector with LEU2 marker for yeast transformation

    Depositor

    John Wyrick

    Article

    Laughery et al Yeast. 2015 Dec;32(12):711-20. doi: 10.1

    Inserts

    Cas9
    Empty
    

    Use

    CRISPR

    Expression

    Yeast

    Available Since

    Aug. 31, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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