We narrowed to 16,608 results for: IGH@

1. mTagRFP-T-Golgi-7

   Plasmid

   #58018

   Purpose

   Localization: Golgi Complex, Excitation: 555, Emission: 584

   Depositor

   Michael Davidson

   Article

   Shaner et al Nat Methods. 2008 Jun;5(6):545-51. doi:

   Insert

   Golgi (B4GALT1 Human)
   

   Use

   Tags

   mTagRFP

   Expression

   Mammalian

   Mutation

   aa 1-82 of NM_001497.3

   Promoter

   CMV

   Available since

   July 31, 2015

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

2. tdTomato-Gamma-Tubulin-17

   Plasmid

   #58099

   Purpose

   Localization: Centrosomes, Excitation: 554, Emission: 581

   Depositor

   Michael Davidson

   Article

   Shaner et al Nat Methods. 2008 Jun;5(6):545-51. doi:

   Insert

   Gamma-Tubulin (TUBG1 Human)
   

   Use

   Tags

   tdTomato

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Dec. 19, 2014

   Availability

   Academic Institutions and Nonprofits only

3. ColorfulCell

   Plasmid

   #62449

   Purpose

   MXS_chaining vector with 6 cassettes

   Depositor

   Pierre Neveu

   Article

   Sladitschek et al PLoS One. 2015 Apr 24;10(4):e0124958. do

   Inserts

   CMV::GTS-Citrine-bGHpA
   CMV::Lyn-Ceruleanx3-bGHpA
   CMV::NLS-TagBFPx3-bGHpA
   CMV::MTS-AzamiGreen-bGHpA
   CMV::iRFP670-SKL-bGHpA
   CMV::ETS-mCherry-KDEL-bGHpA
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   May 8, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pcDNA3-mCardinal

   Plasmid

   #51311

   Purpose

   Expresses far-red fluorescent protein mCardinal in mammalian cells

   Depositor

   Michael Lin

   Article

   Chu et al Nat Methods. 2014 Mar 16. doi: 10.1038/n

   Insert

   mCardinal
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   April 8, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pLV-REPORT(PGKCMV)-nucTomato-hygro

   Plasmid

   #208573

   Purpose

   LV REPORT containing minimal promoter driving monomeric nuclear Tomato followed by an PGK/CMV promoter driving nuclear d2eGFP E2A Hygromycin

   Depositor

   David Bersten

   Article

   Allen et al bioRxiv 2023.11.21.568191

   Inserts

   mnucTomato
   d2nucEGFP
   HygroR
   

   Use

   Lentiviral and Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   PGK/CMV and minP

   Available since

   April 25, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. mTagRFP-T-LaminB1-10

   Plasmid

   #58020

   Purpose

   Localization: Nuclear Envelope, Excitation: 555, Emission: 584

   Depositor

   Michael Davidson

   Article

   Shaner et al Nat Methods. 2008 Jun;5(6):545-51. doi:

   Insert

   LaminB1 (LMNB1 Human)
   

   Use

   Tags

   mTagRFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Nov. 4, 2014

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

7. HIP1R-GFP-FKBP

   Plasmid

   #100752

   Purpose

   Expression of HIP1R with a GFP-FKBP tag in mammalian cells.

   Depositor

   Stephen Royle

   Article

   Clarke et al Nat Commun. 2018 Jul 4;9(1):2604. doi: 1

   Insert

   HIP1R (HIP1R Human)
   

   Use

   Tags

   FKBP and GFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Sept. 27, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pLV_PGK_LTB4R

   Plasmid

   #208087

   Purpose

   Mammalian expression of the wild-type human LTB4 receptor LTB4R.

   Depositor

   Sean R Collins

   Article

   Lundgren et al Sci Signal. 2023 Oct 3;16(805):eadd1845.

   Insert

   LTB4R (LTB4R Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   none

   Promoter

   PGK

   Available since

   Nov. 2, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pCOLA-Gent-EM7-Erv1p-DsbC

   Plasmid

   #202485

   Purpose

   Expresses sulfhydryl oxidase (Erv1p) and disulfide bond isomerase (dsbC) constitutively

   Depositor

   Michael Lynch

   Article

   Hennigan et al Metab Eng. 2024 Sep;85:116-130. doi: 10.

   Insert

   sulfhydryl oxidase (Erv1p) and disulfide bond isomerase (dsbC) (ERV1 Budding Yeast, Escherichia coli)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   EM7

   Available since

   Nov. 14, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pCS2-nCas9n-nanos 3’UTR

    Plasmid

    #62542

    Purpose

    expression of a germ-line targeted, codon optimized Cas9 for genome-editing in zebrafish

    Depositor

    Antonio Giraldez

    Article

    Moreno-Mateos et al Nat Methods. 2015 Aug 31. doi: 10.1038/n

    Insert

    nanos 3' UTR (nanos3 Zebrafish)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    3' UTR only

    Promoter

    SP6

    Available since

    July 28, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. gCH27 (crCD55-4)

    Plasmid

    #217343

    Purpose

    crRNA targeting CD55

    Depositor

    Luke Gilbert

    Article

    Hsiung et al Nat Biotechnol. 2024 May 17. doi: 10.103

    Insert

    crCD55-4 (CD55 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    hU6

    Available since

    March 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pLV-REPORT(PGK/CMV)

    Plasmid

    #172330

    Purpose

    LV REPORT containing minimal promoter driving monomeric nuclear Tomato 2a HSVtk 2a Neo followed by an PGK/CMV promoter driving nuclear d2eGFP E2A Hygromycin

    Depositor

    David Bersten

    Article

    Allen et al bioRxiv 2023.11.21.568191

    Inserts

    monomeric nuclear Tomato
    d2eGFP
    HygR
    

    Use

    Lentiviral and Synthetic Biology

    Tags

    3x SV40 NLS

    Expression

    Mammalian

    Mutation

    Promoter

    PGK/CMV

    Available since

    June 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pmScarlet_alphaTubulin_C1

    Plasmid

    #85045

    Purpose

    In vivo visualization of microtubules (can be used for colocalization studies)

    Depositor

    Dorus Gadella

    Article

    Bindels et al Nat Methods. 2016 Nov 21. doi: 10.1038/n

    Insert

    Alpha-Tubulin (TUBA1B Human)
    

    Use

    Tags

    mScarlet

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Dec. 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pAAV-TRE-NpHR-EYFP

    Plasmid

    #171621

    Purpose

    TRE-NpHR-EYFP reporter

    Depositor

    Hyungbae Kwon

    Article

    Hyun et al Nat Commun. 2022 Dec 12;13(1):7692. doi:

    Insert

    TRE-NpHR-EYFP
    

    Use

    AAV and Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    TetO

    Available since

    Oct. 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. mTagBFP2-Farnesyl-5

    Plasmid

    #55295

    Purpose

    Localization: Plasma Membrane, Excitation: 399, Emission: 456

    Depositor

    Michael Davidson

    Article

    Subach et al PLoS One. 2011;6(12):e28674. Epub 2011 D

    Insert

    Farnesyl (HRAS Human)
    

    Use

    Tags

    mTagBFP2

    Expression

    Mammalian

    Mutation

    encodes final 20 aa of NM_001130442.1

    Promoter

    CMV

    Available since

    Oct. 10, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

16. pSB700

    Plasmid

    #64046

    Purpose

    Lentiviral vector for expressing U6 sgRNA and CAGGS Cerulean fluorescent protein

    Depositor

    George Church

    Article

    Chavez et al Nat Methods. 2015 Mar 2. doi: 10.1038/nm

    Type

    Empty backbone

    Use

    CRISPR, Lentiviral, and Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6, CAGGS

    Available since

    May 15, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pLAMP1-miRFP703

    Plasmid

    #79998

    Purpose

    LAMP1 (Lysosome Membrane) labeling with near-infrared fluorescent protein miRFP703

    Depositor

    Vladislav Verkhusha

    Article

    Shcherbakova et al Nat Commun. 2016 Aug 19;7:12405. doi: 10

    Insert

    LAMP1 (Lamp1 Rat)
    

    Use

    Tags

    miRFP703

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 20, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. nLuc-PTHR1

    Plasmid

    #162577

    Purpose

    Expresses N-terminally NanoLuc-Tagged PTHR1 in mammalian cells

    Depositor

    Sam Gellman

    Article

    Yu et al ACS Chem Biol. 2022 Nov 18;17(11):3148-3

    Insert

    Parathyroid Hormone Receptor 1 (PTH1R Human)
    

    Use

    Tags

    Nanoluc

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 30, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pHAGE-CMV-Luc2-IRES-ZsGreen-W

    Plasmid

    #164432

    Purpose

    Lentiviral vector backbone encoding firefly luciferase and ZsGreen

    Depositor

    Alejandro Balazs

    Article

    Garcia-Beltran et al Cell. 2021 Jan 21;184(2):476-488.e11. do

    Inserts

    Luciferase
    ZsGreen
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pRSFDuet-1_VNp-mNeongreen-DARPinOFF7

    Plasmid

    #182415

    Purpose

    Bacterial expression of Vesicle Nucleating peptide-mNeongreen-DARPinOFF7 fusion

    Depositor

    Dan Mulvihill

    Article

    Eastwood et al Cell Rep Methods. 2023 Feb 2;3(2):100396

    Insert

    VNp-mNeongreen-DARPinOFF7
    

    Use

    Tags

    Vesicle Nucleating peptide (VNp) & mNeongreen

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Feb. 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. AAV2-hSyn-G-Flamp1-mut

    Plasmid

    #188570

    Purpose

    control probe of G-Flamp1, insensitive to cAMP

    Depositor

    Jun Chu

    Article

    Wang et al Nat Commun. 2022 Sep 12;13(1):5363. doi:

    Insert

    G-Flamp1-mut
    

    Use

    AAV

    Tags

    His tag

    Expression

    Mutation

    Promoter

    hSyn

    Available since

    Oct. 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. xCas9(3.7)-ABE(7.10)

    Plasmid

    #108382

    Purpose

    Mammalian expression vector for xCas9(3.7)-ABE

    Depositor

    David Liu

    Article

    Hu et al Nature. 2018 Feb 28. pii: nature26155. d

    Insert

    xCas9(3.7)-ABE(7.10)
    

    Use

    Tags

    NLS

    Expression

    Mammalian

    Mutation

    A262T, R324L, S409I, E480K, E543D, M694I, E1219V

    Promoter

    Available since

    March 28, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pGMC00028 (aka pTS0057)

    Plasmid

    #200832

    Purpose

    AAVS1 guide RNA cloned into the lentiSAM puro backbone

    Depositor

    Raj Chari

    Article

    Yang et al Sci Adv. 2024 Feb 16;10(7):eadj2445. doi

    Insert

    AAVS1 guide RNA
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. TagRFP-T-EEA1

    Plasmid

    #42635

    Depositor

    Silvia Corvera

    Article

    Navaroli et al Proc Natl Acad Sci U S A. 2012 Feb 21;10

    Insert

    EEA1 (EEA1 Human)
    

    Use

    Tags

    TagRFP-T

    Expression

    Mammalian

    Mutation

    none

    Promoter

    CMV

    Available since

    March 1, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pGEX-4T-1 3xHA-MEK1-DD

    Plasmid

    #47576

    Purpose

    Bacterial expression plasmid for GST-3xHA-tagged constitutively active MEK1

    Depositor

    Kevin Janes

    Article

    Bose et al Mol Cell Proteomics. 2013 Mar;12(3):797-

    Insert

    MEK1-DD (Map2k1 Mouse)
    

    Use

    Tags

    3xHA and GST

    Expression

    Bacterial

    Mutation

    Ser218 to Asp, Ser221 to Asp

    Promoter

    Available since

    Aug. 28, 2013

    Availability

    Academic Institutions and Nonprofits only

26. pCRISPR-cBEST

    Plasmid

    #125689

    Purpose

    C to T base editor for actinomycetes

    Depositor

    Tilmann Weber

    Article

    Tong et al Proc Natl Acad Sci U S A. 2019 Oct 8;116

    Insert

    streptomyces codon optimized spCas9n (D10A), modified sgRNA casette, streptomyces codon optimized rAPOBEC1
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    ermE*/tipA

    Available since

    Feb. 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pETNKI-strepII-3C-LIC-Amp

    Plasmid

    #108707

    Purpose

    Expression of a StrepII-tagged target protein with 3C protease cleavage site.

    Depositor

    Titia Sixma

    Article

    Luna-Vargas et al J Struct Biol. 2011 Aug;175(2):113-9. do

    Type

    Empty backbone

    Use

    Tags

    StrepII-3C protease cleavage site

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Aug. 1, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. Tol2-elavl3-GCaMP6s

    Plasmid

    #59531

    Purpose

    Expressed GCaMP6-slow, a calcium indicator, in neurons in zebrafish larva. The plasmid is in Tol2 transposon backbone

    Depositor

    Misha Ahrens

    Article

    Vladimirov et al Nat Methods. 2014 Jul 27. doi: 10.1038/n

    Inserts

    elavl3 promoter (elavl3 Zebrafish)
    GCaMP6s
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 12, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pYLsgRNA-OsU6b

    Plasmid

    #66196

    Purpose

    cloning of sgRNAs for expression in plants

    Depositor

    Yao-Guang Liu

    Article

    Ma et al Mol Plant. 2015 Apr 24. pii: S1674-2052(

    Type

    Empty backbone

    Use

    Cloning vector

    Tags

    Expression

    Mutation

    Promoter

    OsU6b

    Available since

    Aug. 10, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pYPQ-dzCas9-Act3.0

    Plasmid

    #158414

    Purpose

    CRISPR-Act3.0 system containing dzCas9-VP64 fusion protein, T2A linked MS2-SunTag fusion protein, and ScFv-sfGFP-2xTAD activator.

    Depositor

    Yiping Qi

    Article

    Pan et al Nat Plants. 2021 Jul;7(7):942-953. doi:

    Insert

    dzCas9-VP64-T2A-MS2-SunTag-rbcS-E9t-ter-ZmUbi-scFv-sfGFP-2xTAD-GB1-NOS-ter
    

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    July 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pcDNA3.3_LIN28A

    Plasmid

    #26819

    Depositor

    Derrick Rossi

    Article

    Warren et al Cell Stem Cell. 2010 Sep 29. ():.

    Insert

    5'UTR-LIN28A-3'UTR (LIN28A Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 17, 2010

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

32. pX330 _CRISPIE donor B8

    Plasmid

    #172849

    Purpose

    DRS-2 sgRNA expression under a U6 promotor and CRISPIE donor B8 (Zhong et al, eLife 2021), mEGFP translational phase (1-1), excised by DRS-1 or DRS-2 sgRNA (with SpCas9).

    Depositor

    Haining Zhong

    Article

    Zhong et al Elife. 2021 Jun 8;10. pii: 64911. doi: 1

    Insert

    DRS-2 sgRNA and CRISPIE designer exon (phase 1-1) encoding mEGFP
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pAAV-CaMKII-WHaloCaMP1a

    Plasmid

    #205310

    Purpose

    Production of AAV particles encoding WHaloCaMP1a from the CaMKII promoter

    Depositor

    Eric Schreiter

    Article

    Farrants et al bioRxiv. 2023 Jul 19:2023.07.18.549527.

    Insert

    WHaloCaMP1a
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 18, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pSR646

    Plasmid

    #65215

    Purpose

    Baculovirus-mediated rAAV production: Expresses bicistronic AAV-2 rep gene and polycistronic AAV-6 cap gene in Sf9 insect cells

    Depositor

    Robert Kotin

    Article

    Smith et al Mol Ther. 2009 Nov;17(11):1888-96. doi:

    Inserts

    modified AAV-2 rep78
    modified AAV-6 cap
    

    Use

    AAV

    Tags

    Expression

    Insect

    Mutation

    Promoter

    P10 and polyhedrin

    Available since

    May 19, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pZCS36 (Phsp16.41::Cas9(dpiRNA)::tbb-2 ‘3UTR)

    Plasmid

    #193048

    Purpose

    Heatshock expressed Cas9

    Depositor

    Patrick Phillips

    Article

    Stevenson et al Elife. 2023 Jul 4;12:RP84831. doi: 10.75

    Insert

    hsp16.41p::Cas9(dpiRNA)::tbb-2 ‘3UTR
    

    Use

    CRISPR

    Tags

    Expression

    Worm

    Mutation

    Promoter

    hsp16.41p

    Available since

    Sept. 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pHR-CMV-TetO2_HA-BirA-ER

    Plasmid

    #113897

    Purpose

    Mammalian (inducible) expression of HA-tagged ER-resident E. coli biotin ligase

    Depositor

    A. Radu Aricescu

    Article

    Elegheert et al Nat Protoc. 2018 Nov 19. pii: 10.1038/s4

    Insert

    E. coli biotin ligase
    

    Use

    Lentiviral

    Tags

    HA

    Expression

    Mutation

    Promoter

    CMV-MIE-TetO2

    Available since

    Aug. 28, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pFlp-Gm

    Plasmid

    #222335

    Purpose

    Expresses Flp and gentamicin to enable excision of antibiotic resistance cassettes; flp-cl-sacB-pL1V-Lv1-gent-pBBR1-ELT3

    Depositor

    Philip Poole

    Article

    Jorrin et al Microbiome. 2024 May 7;12(1):81. doi: 10

    Inserts

    sacB (sacB B. subtilis)
    flp (flp Budding Yeast)
    cI (cI )
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    cl, flp, and sacB

    Available since

    Nov. 7, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pAAV-hSyn-mScarlet

    Plasmid

    #131001

    Purpose

    AAV vector to drive the expression of mScarlet under the control of human Synapsin promoter

    Depositor

    Karl Deisseroth

    Article

    Marshel et al Science. 2019 Aug 9;365(6453). pii: scie

    Has Service

    AAV1 and AAV8

    Insert

    mScarlet
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    hSyn

    Available since

    Sept. 6, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pRC-CMV-Rev1b

    Plasmid

    #164443

    Purpose

    Encodes HIV-1 Rev accessory protein for pseudovirus production

    Depositor

    Alejandro Balazs

    Article

    Garcia-Beltran et al Cell. 2021 Jan 21;184(2):476-488.e11. do

    Insert

    HIV-1 Rev
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pJT121_GalL_nCDA1-VQRBE3

    Plasmid

    #145086

    Purpose

    Expressing base editor nCDA1-VQRBE3 in yeast cells

    Depositor

    Ralph Bock

    Article

    Tan et al Nat Commun. 2020 Jan 31;11(1):629. doi:

    Insert

    nCDA1-VQRBE3
    

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    spCas9(D10A, D1135V, R1335Q, T1337R)

    Promoter

    GalL

    Available since

    July 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

41. pAAV-hsyn-GRAB_DA2h

    Plasmid

    #140554

    Purpose

    Expresses the 2nd generation high-affinity DA sensor GRAB_DA2h in neurons

    Depositor

    Yulong Li

    Article

    Sun et al Nat Methods. 2020 Nov;17(11):1156-1166.

    Has Service

    AAV9

    Insert

    The 2nd generation DA sensor GRAB_DA2h (high affinity)
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    hSyn

    Available since

    July 29, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

42. pCMV-haA3A-CBE-G-NG

    Plasmid

    #220897

    Purpose

    Express haA3A-CBE-G-NG (with SpCas9-NG variant) in mammalian cells

    Depositor

    Dali Li

    Article

    Yang et al Nat Chem Biol. 2024 Mar 29. doi: 10.1038

    Insert

    haA3A-CBE-G-NG
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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43. pCMV-haA3A-CBE-G

    Plasmid

    #220894

    Purpose

    Express haA3A-CBE-G (with SpCas9) in mammalian cells

    Depositor

    Dali Li

    Article

    Yang et al Nat Chem Biol. 2024 Mar 29. doi: 10.1038

    Insert

    haA3A-CBE-G
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

44. pET28a-TS2126 RNA Ligase

    Plasmid

    #119941

    Purpose

    Express TS2126 RNA ligase in E. coli

    Depositor

    Takashi Ito

    Article

    Miura et al Nucleic Acids Res. 2019 May 22. pii: 549

    Insert

    TS2126 RNA ligase
    

    Use

    Tags

    His6

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    May 23, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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45. pMCB619

    Plasmid

    #171011

    Purpose

    CRISPR sgRNA vector (puro-BFP with BstXI/BlpI digest sites).

    Depositor

    Alice Ting

    Article

    Coukos et al eLife. 2021 Aug 20;10. pii: 69142. doi:

    Insert

    U6:sgRNA_Puro-T2A-BFP
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    mU6

    Available since

    Aug. 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

46. PME-1 pcDNA-StrepIII

    Plasmid

    #119292

    Purpose

    Mammalian overexpression of StrepII-tagged full length PPME1

    Depositor

    Jukka Westermarck

    Article

    Pokharel et al Mol Cell Proteomics. 2015 Dec;14(12):327

    Insert

    PPME1 (PPME1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

47. pmScarlet_H2A_C1

    Plasmid

    #85051

    Purpose

    In vivo visualization of the nucleus (can be used for colocalization studies)

    Depositor

    Dorus Gadella

    Article

    Bindels et al Nat Methods. 2016 Nov 21. doi: 10.1038/n

    Insert

    H2A (H2AC16 Human)
    

    Use

    Tags

    mScarlet

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Dec. 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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48. T7-VEE-mCherry

    Plasmid

    #193355

    Purpose

    Self-replicating RNA vector expressing mCherry fluorescent protein + PuroR

    Depositor

    Hans Schöler

    Article

    MacCarthy et al Cell Stem Cell. 2024 Jan 4;31(1):127-147

    Insert

    mCherry
    

    Use

    Venezuelan equine encephalitis (vee) virus rna re…

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    T7

    Available since

    Dec. 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

49. pYLsgRNA-AtU6-1

    Plasmid

    #66202

    Purpose

    cloning of sgRNAs for expression in plants

    Depositor

    Yao-Guang Liu

    Article

    Ma et al Mol Plant. 2015 Apr 24. pii: S1674-2052(

    Type

    Empty backbone

    Use

    Cloning vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 10, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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50. pcDNA3-mNeptune2.5

    Plasmid

    #51310

    Purpose

    Expresses far-red fluorescent protein mNeptune2.5 in mammalian cells

    Depositor

    Michael Lin

    Article

    Chu et al Nat Methods. 2014 Mar 16. doi: 10.1038/n

    Insert

    mNeptune2.5
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 8, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge