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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 63,812 results for: cloning

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Showing: 4061 - 4080 of 63812 results

  1. mCh-KIF5B*-strep

    Plasmid
    #120164
    Purpose
    Expresses a chimera of mCherry (fluorescent tag), a KIF5B-based anterograde motor and streptavidin
    Depositor
    Juan Bonifacino
    Publication
    Guardia et al PLoS Biol. 2019 May 17;17(5):e3000279. d
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    kinesin family member 5B (Kif5b Mouse)
    Tags
    Streptavidin and mCherry
    Expression
    Mammalian
    Mutation
    Truncated KIF5B: 1-555 aa
    Promoter
    CMV
    Available since
    July 30, 2019
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    120164-plasmid-map-sequence-id-247489

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  3. pLL3.7-EF-EYFP-YAP1_5SA-PolyA

    Plasmid
    #112285
    Purpose
    Fluorescently labels human YAP1-2gamma isoform (504 aa) 5SA active mutant - serines 61, 109, 127, 164 and 397 (also known as 381 in other isoforms)
    Depositor
    Erik Sahai
    Publication
    Ege et al Cell Syst. 2018 Jun 27;6(6):692-708.e13.
    Insert
    EYFP-YAP1 (YAP1 Human)
    Use
    Lentiviral
    Mutation
    Human YAP1-2gamma with 5 Serines (61, 109, 127, 1…
    Promoter
    EF-1 alpha
    Available since
    May 13, 2019
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    112285-plasmid-map-sequence-id-227437

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  8. pBacMam2-DiEx-LIC-C-flag_huntingtin_full-length_Q23

    Plasmid
    #111726
    Purpose
    Baculovirus transfer vector for expression of huntingtin protein in insect and mammalian cellscells as well as in mammalian cells
    Depositor
    Cheryl Arrowsmith
    Publication
    Harding et al J Biol Chem. 2019 Apr 26;294(17):6986-70
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    HTT (HTT Human)
    Use
    Baculovirus expression
    Tags
    FLAG
    Expression
    Mammalian
    Mutation
    Q23 (polyQ repeat)
    Promoter
    CMV and P10
    Available since
    June 14, 2018
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    111726-plasmid-map-sequence-id-212896

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  12. pCMV-BE-VQRCas9

    Plasmid
    #101740
    Purpose
    expression of an optimized Cas9 for base-editing in zebrafish
    Depositor
    Shuo Lin
    Publication
    Zhang et al Nat Commun. 2017 Jul 25;8(1):118. doi: 1
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    rAPOBEC1-XTEN-zCas9(VQR)-UGI-NLS
    Use
    CRISPR
    Expression
    Mammalian
    Mutation
    D10A, VQR mutations
    Promoter
    CMV
    Available since
    Jan. 24, 2018
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    101740-plasmid-map-sequence-id-198315

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  13. pJSC114 - Bacterial expression plasmid for SpCas9 eSpCas9(1.1) variant

    Plasmid
    #101215
    Purpose
    Bacterial expression plasmid for SpCas9 eSpCas9(1.1) variant
    Depositor
    Jennifer Doudna, Keith Joung
    Publication
    Chen et al Nature. 2017 Sep 20. doi: 10.1038/nature
    Insert
    SpCas9 variant K848A/K1003A/R1060A
    Tags
    10x His, MBP, and TEV site
    Expression
    Bacterial
    Mutation
    K848A, K1003A and R1060A
    Promoter
    T7
    Available since
    Oct. 4, 2017
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    101215-plasmid-map-sequence-id-194634

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  15. pCRISPRi_Mxi1_yl_NHEJ

    Plasmid
    #91249
    Purpose
    CRISPRi vector for Yarrowia lipolytica, repressing KU70 and KU80 for enhanced HR
    Depositor
    Ian Wheeldon
    Publication
    Schwartz et al Biotechnol Bioeng. 2017 Aug 19. doi: 10.
    Inserts
    Codon optimized dCas9-Mxi1
    KU70 sgRNA expression cassette
    KU80a sgRNA expression cassette
    KU80b sgRNA expression cassette
    Use
    CRISPR and Synthetic Biology
    Expression
    Yeast
    Promoter
    SCR1'-tRNA and UAS1B8-TEF(136)
    Available since
    Aug. 24, 2017
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    91249-plasmid-map-sequence-id-181276

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  16. STARR-seq luciferase validation vector_ORI_CMV

    Plasmid
    #99312
    Purpose
    Luciferase validation vector with CMV enhancer inserted downstream of the reporter gene. The candidate's enhancer activity is reflected by luciferase activity
    Depositor
    Alexander Stark
    Publication
    Muerdter et al Nat Methods. 2017 Dec 11. pii: nmeth.453
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    CMV enhancer
    Use
    Luciferase; Starr-seq luciferase validation vector
    Expression
    Mammalian
    Available since
    Aug. 17, 2017
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    99312-plasmid-map-sequence-id-190933

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  17. pAsCpf1(TATV)(BB) (pY221)

    Plasmid
    #89354
    Purpose
    Expresses humanized AsCpf1 TATV PAM variant and crRNA guide
    Depositor
    Feng Zhang
    Publication
    Gao et al Nat Biotechnol. 2017 Jun 5. doi: 10.1038
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Cpf1 RVR variant
    Use
    CRISPR
    Tags
    NLS and NLS-3xHA
    Expression
    Mammalian
    Mutation
    S542R/K548V/N552R
    Promoter
    CBh
    Available since
    July 12, 2017
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    89354-plasmid-map-sequence-id-182593

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  18. pGRG36-Kn-PA1-GFP

    Plasmid
    #79088
    Purpose
    pGRG36-Kn-PA1-GFP is a Kanamycine resistance plasmid used to integrate a GFP expression cassette into the chromosome of E.coli, described in Yang et al. 2016 (Pending publication Pubmed ID)
    Depositor
    Frank Rosenzweig
    Publication
    Yang et al Appl Environ Microbiol. 2020 Apr 1;86(8)
    Insert
    GFP
    Use
    Bacterial chromosome integration and expression o…
    Expression
    Bacterial
    Promoter
    PA1
    Available since
    July 12, 2017
    Availability
    Academic Institutions and Nonprofits only
    79088-plasmid-map-sequence-id-182383

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Showing: 4061 - 4080 of 63812 results