We narrowed to 15,978 results for: LEA

1. pGBW-m4046266

   Plasmid

   #145394

   Purpose

   Yeast Expression plasmid for SARS-CoV-2 nsp7

   Depositor

   Benjie Chen, Ginkgo Bioworks

   Insert

   SARS-CoV-2 nsp7 (ORF1ab Severe acute respiratory syndrome coronavirus 2)
   

   Tags

   Cleavable thrombin;3xFLAG

   Expression

   Yeast

   Mutation

   Saccharomyces cerevisiae recode 1

   Promoter

   pTEF1

   Available Since

   April 29, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

2. pGBW-m4046168

   Plasmid

   #145382

   Purpose

   Yeast Expression plasmid for SARS-CoV-2 ORF7b

   Depositor

   Benjie Chen, Ginkgo Bioworks

   Insert

   SARS-CoV-2 ORF7b (ORF7b Severe acute respiratory syndrome coronavirus 2, Budding Yeast)
   

   Tags

   Cleavable thrombin;3xFLAG

   Expression

   Yeast

   Mutation

   wt

   Promoter

   pTEF1

   Available Since

   April 29, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

3. pGBW-m4046544

   Plasmid

   #145408

   Purpose

   Yeast Expression plasmid for SARS-CoV-2 nsp11

   Depositor

   Benjie Chen, Ginkgo Bioworks

   Insert

   SARS-CoV-2 nsp11 (ORF1ab Severe acute respiratory syndrome coronavirus 2, Budding Yeast)
   

   Tags

   Cleavable thrombin;StrepII

   Expression

   Yeast

   Mutation

   wt

   Promoter

   pTEF1

   Available Since

   April 28, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

4. pGBW-m4046358

   Plasmid

   #145396

   Purpose

   Yeast Expression plasmid for SARS-CoV-2 endoRNAse

   Depositor

   Benjie Chen, Ginkgo Bioworks

   Insert

   SARS-CoV-2 endoRNAse (ORF1ab Severe acute respiratory syndrome coronavirus 2, Budding Yeast)
   

   Tags

   Cleavable thrombin;StrepII

   Expression

   Yeast

   Mutation

   wt

   Promoter

   pTEF1

   Available Since

   April 28, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

5. pGBW-m4046208

   Plasmid

   #145557

   Purpose

   Yeast Expression plasmid for SARS-CoV-2 nsp4

   Depositor

   Benjie Chen, Ginkgo Bioworks

   Insert

   SARS-CoV-2 nsp4 (ORF1ab Severe acute respiratory syndrome coronavirus 2, Budding Yeast)
   

   Tags

   Cleavable thrombin;StrepII

   Expression

   Yeast

   Mutation

   wt

   Promoter

   pGAL1

   Available Since

   April 28, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

6. pGBW-m4046347

   Plasmid

   #145546

   Purpose

   Yeast Expression plasmid for SARS-CoV-2 nsp9

   Depositor

   Benjie Chen, Ginkgo Bioworks

   Insert

   SARS-CoV-2 nsp9 (ORF1ab Severe acute respiratory syndrome coronavirus 2, Budding Yeast)
   

   Tags

   Cleavable thrombin;StrepII

   Expression

   Yeast

   Mutation

   wt

   Promoter

   pGAL1

   Available Since

   April 28, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

7. pGBW-m4046525

   Plasmid

   #145523

   Purpose

   Yeast Expression plasmid for SARS-CoV-2 nsp7

   Depositor

   Benjie Chen, Ginkgo Bioworks

   Insert

   SARS-CoV-2 nsp7 (ORF1ab Severe acute respiratory syndrome coronavirus 2, Budding Yeast)
   

   Tags

   Cleavable thrombin;StrepII

   Expression

   Yeast

   Mutation

   wt

   Promoter

   pGAL1

   Available Since

   April 28, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

8. pGBW-m4046290

   Plasmid

   #145522

   Purpose

   Yeast Expression plasmid for SARS-CoV-2 nsp10

   Depositor

   Benjie Chen, Ginkgo Bioworks

   Insert

   SARS-CoV-2 nsp10 (ORF1ab Severe acute respiratory syndrome coronavirus 2, Budding Yeast)
   

   Tags

   Cleavable thrombin;StrepII

   Expression

   Yeast

   Mutation

   wt

   Promoter

   pGAL1

   Available Since

   April 28, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

9. pGBW-m4046466

   Plasmid

   #145466

   Purpose

   Yeast Expression plasmid for SARS-CoV-2 nsp6

   Depositor

   Benjie Chen, Ginkgo Bioworks

   Insert

   SARS-CoV-2 nsp6 (ORF1ab Severe acute respiratory syndrome coronavirus 2, Budding Yeast)
   

   Tags

   Cleavable thrombin;StrepII

   Expression

   Yeast

   Mutation

   wt

   Promoter

   pTEF1

   Available Since

   April 27, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

10. pGBW-m4046218

    Plasmid

    #145498

    Purpose

    Yeast Expression plasmid for SARS-CoV-2 nsp2

    Depositor

    Benjie Chen, Ginkgo Bioworks

    Insert

    SARS-CoV-2 nsp2 (ORF1ab Severe acute respiratory syndrome coronavirus 2, Budding Yeast)
    

    Tags

    Cleavable thrombin;StrepII

    Expression

    Yeast

    Mutation

    wt

    Promoter

    pGAL1

    Available Since

    April 27, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

11. phSOD1-L126X-N’His

    Plasmid

    #117707

    Purpose

    mutation on stability/solubility of SOD1

    Depositor

    Elizabeth Fisher

    Insert

    SOD1 (SOD1 Human)
    

    Tags

    His tag with Thrombin cleavage site

    Expression

    Bacterial

    Mutation

    L126X

    Promoter

    T7

    Available Since

    Nov. 15, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. phSOD1-D101G-N’His

    Plasmid

    #117704

    Purpose

    mutation on stability/solubility of SOD1

    Depositor

    Elizabeth Fisher

    Insert

    SOD1 (SOD1 Human)
    

    Tags

    His tag with Thrombin cleavage site

    Expression

    Bacterial

    Mutation

    D101G

    Promoter

    T7

    Available Since

    Nov. 15, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pGAT

    Plasmid

    #112587

    Purpose

    Used for expression of protein in E. coli as Thrombin cleavable 6His-tag-GST fusions with a blunt end restriction site immediately after thrombin recognition sequence

    Depositor

    Johan Peränen, Marko Hyvönen

    Article

    Peranen et al Anal Biochem. 1996 May 1;236(2):371-3.

    Type

    Empty backbone

    Tags

    6His-GST

    Expression

    Bacterial

    Available Since

    Sept. 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pST50Trc4-HPCDHFR

    Plasmid

    #63993

    Purpose

    Position 4 transfer plasmid for pST44 polycistronic plasmid suite; N-term non-cleavable single tag; contains DHFR gene in BamHI-NgoMIV cassette as positive control

    Depositor

    Song Tan

    Article

    Tan et al Protein Expr Purif. 2005 Apr;40(2):385-9

    Type

    Empty backbone

    Tags

    Protein C (HPC)

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Dec. 18, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pST50Trc3-HPCDHFR

    Plasmid

    #63975

    Purpose

    Position 3 transfer plasmid for pST44 polycistronic plasmid suite; N-term non-cleavable single tag; contains DHFR gene in BamHI-NgoMIV cassette as positive control

    Depositor

    Song Tan

    Article

    Tan et al Protein Expr Purif. 2005 Apr;40(2):385-9

    Type

    Empty backbone

    Tags

    Protein C (HPC)

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Dec. 17, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pST50Trc3-FLAGDHFR

    Plasmid

    #63974

    Purpose

    Position 3 transfer plasmid for pST44 polycistronic plasmid suite; N-term non-cleavable single tag; contains DHFR gene in BamHI-NgoMIV cassette as positive control

    Depositor

    Song Tan

    Article

    Tan et al Protein Expr Purif. 2005 Apr;40(2):385-9

    Type

    Empty backbone

    Tags

    FLAG

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Dec. 17, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pST50Trc4-STRDHFR

    Plasmid

    #63991

    Purpose

    Position 4 transfer plasmid for pST44 polycistronic plasmid suite; N-term non-cleavable single tag; contains DHFR gene in BamHI-NgoMIV cassette as positive control

    Depositor

    Song Tan

    Article

    Tan et al Protein Expr Purif. 2005 Apr;40(2):385-9

    Type

    Empty backbone

    Tags

    Strep II (STR)

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Nov. 10, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pST50Trc2-HPCDHFR

    Plasmid

    #63957

    Purpose

    Position 2 transfer plasmid for pST44 polycistronic plasmid suite; N-term non-cleavable single tag; contains DHFR gene in BamHI-NgoMIV cassette as positive control

    Depositor

    Song Tan

    Article

    Tan et al Protein Expr Purif. 2005 Apr;40(2):385-9

    Type

    Empty backbone

    Tags

    Protein C (HPC)

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Oct. 7, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pST50Trc1-HPCDHFR

    Plasmid

    #63939

    Purpose

    Position 1 transfer plasmid for pST44 polycistronic plasmid suite; N-term non-cleavable single tag; contains DHFR gene in BamHI-NgoMIV cassette as positive control

    Depositor

    Song Tan

    Article

    Tan et al Protein Expr Purif. 2005 Apr;40(2):385-9

    Type

    Empty backbone

    Tags

    Protein C (HPC)

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Aug. 25, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. 3HX4

    Plasmid

    #60137

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Cheryl Arrowsmith

    Insert

    TgCDPK4:MAC03F-A01:C208088
    

    Tags

    6xHis and TEV cleavage site

    Expression

    Bacterial

    Mutation

    See comments

    Promoter

    T7

    Available Since

    Oct. 22, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits

21. pGGD006

    Plasmid

    #48836

    Purpose

    Provides the SV40 nuclear localization signal as GreenGate C-terminal tag module.

    Depositor

    Jan Lohmann

    Article

    Lampropoulos et al PLoS One. 2013 Dec 20;8(12):e83043. doi:

    Insert

    SV40 NLS
    

    Use

    Golden gate compatible cloning vector

    Available Since

    Jan. 7, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. L3MBTL1 (3P8H)

    Plasmid

    #28265

    Depositor

    Cheryl Arrowsmith

    Insert

    L3MBTL1 (L3MBTL1 Human)
    

    Tags

    His tag with TEV cleavage

    Expression

    Bacterial

    Mutation

    contains amino acid residues 200-530.

    Available Since

    April 18, 2012

    Availability

    Industry, Academic Institutions, and Nonprofits

23. pLIC.B2.MPN330

    Plasmid

    #11536

    Depositor

    Sung-Hou Kim

    Article

    Lemesle-Varloot et al J Steroid Biochem Mol Biol. 1992 Mar . 4

    Insert

    FAD synthetase
    

    Tags

    HisTag, non-cleavable

    Expression

    Bacterial

    Available Since

    April 10, 2006

    Availability

    Academic Institutions and Nonprofits only

24. pMT Ptp69D-GFP

    Plasmid

    #126663

    Purpose

    Inducible expression of Ptp69D-GFP in S2 cells

    Depositor

    Ron Vale

    Article

    Williamson et al J Cell Biol. 2018 Nov 5;217(11):3977-399

    Insert

    Ptp69D
    

    Expression

    Insect

    Mutation

    A53S, K105N, and A98T- please see depositor comme…

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pCas9

    Plasmid

    #42876

    Purpose

    Bacterial expression of Cas9 nuclease, tracrRNA and crRNA guide

    Depositor

    Luciano Marraffini

    Article

    Jiang et al Nat Biotechnol. 2013 Jan 29. doi: 10.103

    Insert

    tracr/Cas9
    

    Use

    CRISPR; E.coli

    Expression

    Bacterial

    Available Since

    Feb. 26, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pFUW H2B-mTagBFP2-P2A-PuroR

    Plasmid

    #239552

    Purpose

    Expression and nuclear localization of mTagBFP2

    Depositor

    Joel Blanchard, Tim Ahfeldt

    Article

    et al iScience 28 (2025)

    Insert

    mTagBFP2
    

    Use

    Lentiviral

    Promoter

    UbC

    Available Since

    June 30, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pFUW H2B-mEmerald-P2A-PuroR

    Plasmid

    #239553

    Purpose

    Expression and nuclear localization of mEmerald

    Depositor

    Joel Blanchard, Tim Ahfeldt

    Article

    et al iScience 28 (2025)

    Insert

    mEmerald
    

    Use

    Lentiviral

    Promoter

    UbC

    Available Since

    June 30, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. WT FL USP7

    Plasmid

    #227214

    Purpose

    Expresses human USP7 with a N-terminal cleavable 6His tag in E. coli

    Depositor

    Irina Bezsonova

    Insert

    USP7 (Ubiquitin carboxyl-terminal hydrolase 7) (USP7 Human)
    

    Tags

    6His

    Expression

    Bacterial

    Promoter

    T7-promoter

    Available Since

    Nov. 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. NLS-LgBiT-FRB

    Plasmid

    #183403

    Purpose

    Expresses nuclear-localized LgBiT-FRB

    Depositor

    Alice Ting

    Article

    Erdenee et al ACS Synth Biol. 2022 Mar 18;11(3):1086-1

    Insert

    LgBiT-FRB
    

    Tags

    SV40 NLS

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    May 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pRSETA_crE6

    Plasmid

    #172928

    Purpose

    Crowding FRET sensor with mCerulean3 and mCitrine for E. coli expression. Linker comprises 1 helix and 2 GSG linkers

    Depositor

    Arnold Boersma

    Article

    Liu et al Biophys J. 2017 May 9;112(9):1929-1939.

    Insert

    CrowdingSensorE6
    

    Tags

    His

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Aug. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. V5-BS2-NES (Cytosol)

    Plasmid

    #220341

    Purpose

    Mammalian expression vector BS2 in cytoplasm

    Depositor

    Bryan Dickinson

    Article

    Pani et al Nat Chem. 2024 Apr 9. doi: 10.1038/s4155

    Insert

    BS2
    

    Tags

    NES (Nuclear exclusion sequence) and V5 tag

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    June 26, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

32. pEGFP-C1 EGFP-3XNLS

    Plasmid

    #58468

    Purpose

    Expresses a nuclear-targeted eGFP on a CMV promoter

    Depositor

    Dyche Mullins

    Article

    Belin et al Mol Biol Cell. 2013 Apr;24(7):982-94. do

    Insert

    3X NLS with 3X stop codons
    

    Tags

    EGFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    April 24, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. mAG-GAL3

    Plasmid

    #62734

    Purpose

    Mammalian vesicle leakage reporter

    Depositor

    Niels Geijsen

    Article

    D'Astolfo et al Cell. 2015 Apr 23;161(3):674-690. doi: 1

    Insert

    mAG-Galectin 3
    

    Use

    Lentiviral

    Tags

    mAzami-Green

    Expression

    Mammalian

    Promoter

    EF1-Alpha

    Available Since

    April 24, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pET15-UbE2D1

    Plasmid

    #15782

    Depositor

    Wade Harper

    Article

    Jin et al Nature. 2007 Jun 28. 447(7148):1135-8.

    Insert

    Ubiquitin conjugating enzyme UbE2D1 (UBE2D1 Human)
    

    Tags

    6x His, T7 tag, and Xpress tag (enterokinase clea…

    Expression

    Bacterial

    Available Since

    Sept. 28, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pNLS-iRFP713

    Plasmid

    #45468

    Depositor

    Vladislav Verkhusha

    Article

    Shcherbakova et al Nat Methods. 2013 Jun 16. doi: 10.1038/n

    Insert

    iRFP713
    

    Tags

    nuclear localization signal

    Expression

    Mammalian

    Mutation

    T2A, S13L, A92T, V104T, V114I, E161K, E193K, F198…

    Promoter

    CMV

    Available Since

    June 17, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pMEXC3H

    Plasmid

    #110080

    Purpose

    Analogous to pMINTC3H, but containing oriM for episomal replication in mycobacteria.

    Depositor

    Markus Seeger

    Article

    Arnold et al Sci Rep. 2018 Jun 22;8(1):9539. doi: 10.

    Type

    Empty backbone

    Tags

    3C cleavage site - His10 Tag

    Expression

    Bacterial

    Available Since

    June 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pCMVMagFRET-1

    Plasmid

    #50742

    Purpose

    Expresses MagFRET-1 in the cytosol of mammalian cells. FRET sensor based on Cerulean/Citrine for Mg2+ imaging. Sensor does not respond to normal cytosolic Ca2+ levels.

    Depositor

    Maarten Merkx

    Article

    Lindenburg et al PLoS One. 2013 Dec 2;8(12):e82009. doi:

    Insert

    gene encoding for MagFRET-1
    

    Expression

    Mammalian

    Available Since

    Jan. 16, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pNLS-iRFP670

    Plasmid

    #45466

    Depositor

    Vladislav Verkhusha

    Article

    Shcherbakova et al Nat Methods. 2013 Jun 16. doi: 10.1038/n

    Insert

    iRFP670
    

    Tags

    nuclear localization signal

    Expression

    Mammalian

    Mutation

    T2A, S45T, V116I, D124E, M170L, K180M, D202L, I20…

    Promoter

    CMV

    Available Since

    June 17, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. JDW 1042 (pLenti6.2_CMV_V5_TCF7L1_P2A_H2A_mCherry)

    Plasmid

    #233540

    Purpose

    A CMV driven human TCF7L1 followed by a P2A cleavage peptide and an H2A mCherry fusion protein.

    Depositor

    Joshua Wythe

    Article

    Cantu Gutierrez et al bioRxiv 2021.11.15.468651

    Insert

    TCF7L1 (TCF7L1 Human)
    

    Use

    Lentiviral

    Tags

    V5

    Available Since

    March 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pcDNA4-ZfCry4

    Plasmid

    #136242

    Purpose

    Protein expression of Zebrafish (Zf) cytochrome 4 with a C terminal FLAG tag

    Depositor

    Aziz Sancar

    Article

    Ozturk et al Biochemistry. 2009 Sep 15;48(36):8585-93

    Insert

    ZfCry4
    

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    D196H- Please see depositor comments below

    Available Since

    Jan. 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

41. pJExpress414_GmPOPB

    Plasmid

    #92234

    Purpose

    Express wild type prolyl oligopeptidase from Galerina marginata

    Depositor

    James Naismith

    Article

    Czekster et al Biochemistry. 2017 Apr 18;56(15):2086-20

    Insert

    Prolyl oligopeptidase B from Galerina marginata
    

    Tags

    6x-Histag, TEV cleavage site

    Expression

    Bacterial

    Mutation

    none

    Promoter

    T7 promoter

    Available Since

    Aug. 28, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

42. sox10:mCherry

    Plasmid

    #98695

    Purpose

    I-SceI meganuclease-mediated mCherry transgenesis in zebrafish

    Depositor

    Thomas Look

    Article

    Ki et al Oncogene. 2017 Feb 23;36(8):1058-1068. d

    Insert

    mCherry
    

    Use

    Zebrafish expression

    Available Since

    July 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

43. pBA2219

    Plasmid

    #38152

    Depositor

    David Waugh

    Article

    Austin et al Protein Expr Purif. 2012 Mar;82(1):116-2

    Insert

    DsbC (dsbC E. coli)
    

    Tags

    N-terminal leader peptide (cloning artifact)

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Aug. 21, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

44. GCEPIA1-SNAPER

    Plasmid

    #172659

    Purpose

    Detect the real-time ER calcium store and local calcium microdomains in live cells.

    Depositor

    Pingyong Xu

    Article

    Luo et al Biophys Rep (2019) 5, 31–42

    Insert

    calmodulin
    

    Expression

    Mammalian

    Mutation

    Please see depositor comments

    Available Since

    Aug. 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

45. pcDNA5/FRT/TO_HaloTag7_LamB1

    Plasmid

    #175528

    Purpose

    Expression of HaloTag7 at the nuclear lamina of the mammalian cells

    Depositor

    Kai Johnsson

    Article

    Frei et al Nat Methods. 2021 Dec 16. pii: 10.1038/s

    Insert

    HaloTag7-LamB1
    

    Expression

    Mammalian

    Available Since

    Dec. 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

46. pREXC3GH

    Plasmid

    #47077

    Purpose

    FX cloning Lactococcus lactis expression vector (VBEx) with nisin promoter and C-terminal 3C protease cleavage site, GFP and 10x His tags

    Depositor

    Eric Geertsma, Raimund Dutzler

    Article

    Geertsma et al Biochemistry. 2011 Apr 19;50(15):3272-8.

    Type

    Empty backbone

    Use

    Lactococcus lactis expression

    Tags

    3C protease site, GFP, and His

    Promoter

    NIS (nisin)

    Available Since

    Jan. 15, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

47. pKM493

    Plasmid

    #140191

    Purpose

    ORBIT integrating plasmid for C-terminal tagging with cleavable EGFP.

    Depositor

    Kenan Murphy

    Article

    Murphy et al MBio. 2018 Dec 11;9(6). pii: mBio.01467-

    Insert

    TEV-Flag-Gly4-eGFP
    

    Tags

    Flag + eGFP

    Expression

    Bacterial

    Promoter

    PGroEL

    Available Since

    Jan. 2, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

48. pNLS-iRFP720

    Plasmid

    #45467

    Depositor

    Vladislav Verkhusha

    Article

    Shcherbakova et al Nat Methods. 2013 Jun 16. doi: 10.1038/n

    Insert

    iRFP720
    

    Tags

    nuclear localization signal

    Expression

    Mammalian

    Mutation

    T2A, S13L, A92T, V104T, V114I, E161K, E180S, Y193…

    Promoter

    CMV

    Available Since

    June 17, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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49. LeGO-lnc

    Plasmid

    #80624

    Purpose

    Lentiviral lncRNA Overexpression

    Depositor

    Jan-Henning Klusmann

    Article

    Emmrich et al Mol Cancer. 2014 Jul 15;13:171. doi: 10.

    Insert

    empty control
    

    Use

    Lentiviral

    Promoter

    SFFV

    Available Since

    Sept. 9, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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50. pGWT35S-Citrine-NLS

    Plasmid

    #182583

    Purpose

    Transient expression of Citrine-NLS in plant cell (Nucleus)

    Depositor

    Yutaka Kodama

    Article

    Osaki et al PeerJ. 2017 Sep 7;5:e3779. doi: 10.7717/

    Insert

    NLS
    

    Tags

    Citrine

    Expression

    Plant

    Promoter

    CaMV 35S promoter

    Available Since

    May 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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